Single-cell and spatial-resolved profiling reveals cancer-associated fibroblast heterogeneity in colorectal cancer metabolic subtypes by Youpeng Wang, Xingfeng Qiu, Qinghai Li, Jiale Qin, Lvlan Ye, Xiang Zhang, Xingxiang Huang, Xiangqiong Wen, Ziyang Wang, Weiling He, Yuqin Di, Qi Zhou

“…Further exploration of CRC subtypes and interactions among tumor-specific clusters will facilitate the development of personalized treatment strategies. Methods Single-cell RNA sequencing and bulk RNA sequencing datasets were integrated to determine CRC metabolic subtypes by hierarchical clustering. …”
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Heterogeneous transfer learning model for improving the classification performance of fNIRS signals in motor imagery among cross-subject stroke patients by Jin Feng, YunDe Li, ZiJun Huang, Yehang Chen, SenLiang Lu, RongLiang Hu, QingHui Hu, YuYao Chen, XiMiao Wang, Yong Fan, Jing He

“…This study proposes a Cross-Subject Heterogeneous Transfer Learning Model (CHTLM) to enhance the generalization of MI-fNIRS signal classification in stroke patients.MethodsCHTLM leverages labeled electroencephalogram (EEG) data from healthy individuals as the source domain. …”
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Enhancing fibroblast–epithelial cell communications: Serpine2 as a key molecule in Fusobacterium nucleatum–promoted colon cancer by Xueke Li, Xueke Li, Simin Luo, Yifang Jiang, Qiong Ma, Fengming You, Fengming You, Qixuan Kuang, Xi Fu, Chuan Zheng

“…Three machine learning algorithms were employed to identify characteristic genes. Clinical tissue samples and external datasets, along with in vitro co-culture experiments, were utilized to validate these findings.ResultsFollowing Fn intervention, there was an observed increase in the fibroblast iso-cellular ratio and interaction levels. …”
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Gram-positive probiotics improves acetaminophen-induced hepatotoxicity by inhibiting leucine and Hippo-YAP pathway by Wenkang Gao, Gang Wang, Hang Yuan, Yue Chen, Jiake Che, Zilu Cheng, Liuying Chen, Li Zhang, Yuanqing Zhu, Xin Liu, Ao Liu, Quancheng Yang, Peng Cao, Wei Qian, Weiyan Huang, Bernd Schnabl, Ling Yang, Huikuan Chu

“…We aimed to investigate a probiotic mixture comprising Bifidobacterium Longum, Streptococcus thermophilus, and Lactobacillus delbrueckii subspecies bulgaricus (BSL) in mitigating acetaminophen induced liver injury (AILI), and to elucidate the underlying mechanisms. Methods Gut bacterial communities were analyzed in fecal samples from patients with DILI and healthy controls. …”
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Safety and efficacy of oral edible bird’s nest supplementation: Anti-inflammatory and immunomodulatory benefits for Arabian race stallions during exercise by Khalid Obaid AL-Khaldi, Khalid Hamed Al-Ruzaiqi, Abdul Salam Babji, Seng Joe Lim, Mohammed Babatunde Sadiq, Nurhusien Yimer

“…Key objectives included assessing EBN’s impact on hepatic and renal function, hematological profiles, and sialic acid (SA) levels. Materials and Methods: Two experiments were conducted on 18 Arabian stallions. …”
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Connectivity guided intermittent theta burst stimulation versus repetitive transcranial magnetic stimulation in moderately severe treatment resistant depression by Richard Morriss, Lucy Webster, Luke Ingram, Mohamed Abdelghani, Adriana Anton, Shaun Barber, Peter Bates, Paul Briley, Clement Boutry, Cassandra Brookes, Edward Cox, Beth Hall, Marilyn James, Matthew Keane, Micheal Kurkar, Sudheer Lankappa, Peter Liddle, Hamish McAllister-Williams, Hyerin Oh, Alexander O’Neill-Kerr, Jehill Parikh, Stefan Pszczolkowski, Ana Suazo Di Paola, Louise Thomson, Yvette Walters, Dorothee Auer

“…Statistical analysis Primary analysis of the primary outcome used the intention-to-treat (ITT) population with the multiple imputation technique being implemented to deal with missing data in instances where participants were missing any of the HDRS-17 scores. Qualitative methods and analysis A purposive sample was collected of participants and non-participants to explore facilitators and barriers to participation in the trial, and the acceptability of the trial treatments. …”
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Effect of Substrate Temperature on Bead Track Geometry of 316L in Directed Energy Deposition: Investigation and Regression Modeling by Deviprasad Chalicheemalapalli Jayasankar, Stefan Gnaase, Dennis Lehnert, Artur Walter, Robin Rohling, Thomas Tröster

“…A specialized experimental setup, integrated within a DED machine, facilitates the controlled thermal conditioning of sample sheets. Using Design of Experiments (DoE) methods, individual bead marks are generated and analyzed to assess geometric characteristics. …”
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Identification of Anoikis‐Related Genes in Gastric Cancer: Bioinformatics and Experimental Validation by Chao Song, Wenbo Liu, Xiaoyu Wang, Xin Liu, Zhiran Yang, Yingying Wang, Qun Zhao, Yong Li, Mingming Zhang, Bibo Tan

“…ABSTRACT Introduction Distant metastasis is the main reason for the poor prognosis of gastric cancer, and anoikis refers to the cell death caused when cells detach from the extracellular matrix or adhere in incorrect locations, playing an important role in the distant metastasis of gastric cancer. Methods Download the TCGA‐STAD dataset and the anoikis gene set, and filter out the differentially expressed anoikis genes. …”
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A Pilot Study: Maternal Undernutrition Programs Energy Metabolism and Alters Metabolic Profile and Morphological Characteristics of Skeletal Muscle in Postnatal Beef Cattle by Daichi Nishino, Taketo Haginouchi, Takeshi Shimogiri, Susumu Muroya, Kenji Kawabata, Saki Urasoko, Ichiro Oshima, Shinobu Yasuo, Takafumi Gotoh

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Integrated bioinformatics and functional studies identify CDK9 as a potential prognostic biomarker and therapeutic target in AML by Zhibin Xie, Yang Xia, Zhongyu Li, Mengmeng Zhang, Yuanyuan Tan, Yuqing Han, Meng Wang, Pingping Zhang, Jiajia Li

“…Results Bioinformatics analysis revealed significantly elevated CDK9 expression in AML samples, which correlated with poor patient prognosis. …”
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Epoxy composites modified with coal tar by Vladimir T. Erofeyev, Andrey D. Bogatov, Irina E. Kondakova, Viktor I. Ivlev, Vyacheslav A. Yudin, Viktor V. Afonin

“…It has been revealed that nanoscale particles are one of the potential modifiers of the microstructure of composites. Materials and methods. As part of the experiment, composites were studied, in which ED-16 epoxy resin modified with coal tar was used as the main binder. …”
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Problematic Online Dating: Systematic Review of Definitions, Correlates, and Study Designs by Marina F Thomas, Sylvia Dörfler, Gloria Mittmann, Verena Steiner-Hofbauer

“…ObjectiveThis systematic literature review synthesized quantitative research on problematized online dating behaviors with a specific focus on (1) definitions and measurement of problematic dating app use, (2) the examined adverse correlates, and (3) study designs. MethodsFrom 16 databases, we identified 263 reports related to problematic online dating. …”
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Histidine metabolism drives liver cancer progression via immune microenvironment modulation through metabolic reprogramming by Pengcheng Liu, Fuxin Huang, Peixu Lin, Jiayao Liu, Pincheng Zhou, Jie Wang, Huanhuan Sun, Fan Xing, Haiqing Ma

“…In hepatocellular carcinoma (HCC), dysregulated histidine metabolism may promote tumor growth and immune evasion, although the specific mechanisms remain poorly understood. Methods Using single-cell RNA sequencing, the expression patterns of histidine metabolism–related genes were evaluated across different cell types in HCC samples. …”
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Mitochondria-targeted antioxidant mitoquinone mitigates vitrification-induced damage in mouse ovarian tissue by maintaining mitochondrial homeostasis via the p38 MAPK pathway by Tianqi Du, Han Su, Dan Cao, Qingxia Meng, Ming Zhang, Zhenxing Liu, Hong Li

“…This study aims to investigate the protective effects and potential mechanisms of Mitoquinone (MitoQ), a mitochondria-targeted derivative of the antioxidant ubiquinone, during the vitrification of ovarian tissue in mice. Methods KGN cells were treated with various concentrations (0.1, 1, 10, and 50 μM) of MitoQ to determine the optimal concentration. …”
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Immunological effects of CD19.CAR-T cell therapy in systemic sclerosis: an extended case study by Maren Claus, Merle Freitag, Meike Ewald, Lea Rodon, Franca Deicher, Carsten Watzl, Philipp Kolb, Hanns-Martin Lorenz, Michael Schmitt, Wolfgang Merkt

“…Here we investigated effects of CAR-T cells on the innate immune system which is an important contributor to pathology in SSc. Methods Longitudinal analysis of peripheral blood mononuclear cells from an Scl70 + SSc patient treated with CAR-T cells sampled over 18 months by 29-color spectral flow cytometry, in vitro experiments using sera from patient cohorts. …”
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3-Hydroxybutyrate, a metabolite in sustaining neuronal cell vitality: a mendelian randomization and in vitro experimentation by Xiaoling Hu, Yu Lin, Kaiwen Huang, HuiLin Xu, Changmei Huang Fu, Jiayin Ou, Xiude Fan, Zhe Li, Jiansong Fang, Shuhuan Fang

“…However, intermediary metabolites mediating these associations remain elusive. Methods Employing a two-sample and a mediation Mendelian randomization (MR) analysis of the IEU OpenGWAS database, involving 383,476 participants from a genome-wide association study (GWAS) and an exome-wide association study (ExWAS), we identified intermediary metabolites linking mtDNA-CN and Tau.Meanwhile, the effects of mediating metabolites on HT22 cell viability and its mitochondrial morphology were also assessed in conjunction with in vitro experiments. …”
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THE INTENSITY OF BIOFILM FORMATION ON TITANIUM AND ZIRCONIA ABUTMENTS DURING TREATMENT WITH IMPLANT-SUPPORTED PROSTHESES by S.-R. R. Ghot, M.M. Ugrin, O.M. Fal, A.Ya. Baryliak, M.A. Panas

“…We studied the bacterial colony formation on these samples. Experiment showed different adhesion properties of the bacteria depending on the surface character of the experimental material. …”
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CXCL12/CXCR4 axis governs Treg spatial dominance over CD8+ T cells via IL-2 sequestration: a dual therapeutic target in prostate cancer by Junyi Li, Long Zhang, Ruoyang Liu, Changwen Xu, HuiHui Tang, Yunfei Zou, Qingfei Cao, Weichao Huang

“…The mechanisms underlying immune suppression and evasion within the tumor microenvironment (TME) of PCa remain inadequately understood.MethodsA comprehensive analysis of the immune environment in PCa was conducted using combined single-cell and spatial transcriptomic approaches, encompassing samples from healthy tissue, adjacent normal tissue, and localized tumors. …”
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Multi-omics analysis unveils a four-gene prognostic signature in esophageal squamous carcinoma and the therapeutic potential of PKP1 by Xiuzhi Zhang, Zhi Wang, Yutong Zhao, Hua Ye, Tiandong Li, Han Wang, Guiying Sun, Feifei Liang, Liping Dai, Peng Wang, Xiaoli Liu

“…Effective classification for patient stratification and identifying reliable markers for prognosis prediction and treatment choice are crucial. Methods Integration of single-cell RNA-sequencing (RNA-seq) and bulk RNA-seq analyses were used to characterize ESCC. …”
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Integrated Bioinformatics and Experimental Validation Reveal Macrophage Polarization-Related Biomarkers for Osteoarthritis Diagnosis by He Q, Liu L, Hu X, Lin L, Song Z, Xia Y, Lin Q, Wei J, Li S

“…Additionally, various analyses, including immune infiltration, gene set enrichment analysis, competing endogenous RNA (ceRNA) construction, and drug prediction, were conducted. Finally, clinical samples were clinically validated through RT-qPCR (OA: Control = 10: 5) and IHC (6: 5) experiments.Results: Three hub genes (MYC, SIK1, and NFIL3) were identified, and the diagnostic model constructed using them demonstrated good diagnostic efficacy in both internal and external datasets (internal AUC = 0.965, external AUC = 0.847). …”
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