Precarity and preparedness during the SARS‐CoV‐2 pandemic: A qualitative service evaluation of maternity healthcare professionals by Kaat De Backer, Jeremy M. Brown, Abigail Easter, Nina Khazaezadeh, Daghni Rajasingam, Jane Sandall, Laura A. Magee, Sergio A. Silverio

“…Material and methods A qualitative interview service evaluation was undertaken at a single maternity service in an NHS Trust, South London. …”
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Ex vivo myolysis with dual wavelengths diode laser system: macroscopic and histopathological examination by Maurizio Nicola D’Alterio, Francesco Scicchitano, Daniela Fanni, Gavino Faa, Antonio Simone Laganà, Marco Noventa, Felice Sorrentino, Luigi Nappi, Stefano Angioni

“…All the myomas were used for the experiment after a total hysterectomy and their excision from the fresh uteri. …”
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Association between cancer-associated fibroblasts and prognosis of neoadjuvant chemoradiotherapy in esophageal squamous cell carcinoma: a bioinformatics analysis based on single-ce... by Zhao Huang, Zhuangzhuang Cong, Jing Luo, Bingmei Qiu, Kang Wang, Chuan Gao, Yang Xu, Nan Yang, Zhiqiang Zou, Liwen Hu, Yi Shen

“…Marker genes of myofibroblastic CAFs (myCAFs) were employed to establish a prognostic model and verify its application in other datasets. Other experiments were conducted on clinical samples to explore potential ESCC risk-related genes. …”
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Influencing patterns of low-temperature nitrogen on the pore structure and oxidation characteristics of lignite by Shuliang XIE, Gang WANG, Qingdong QU, Hao LIU, Lulu SUN

“…This tends to affect the prediction and forecast of the secondary oxidation of residual coals in goaves after low-temperature nitrogen injection ends. MethodsUsing experiments with an ultra-depth 3D digital microscope and a confocal laser scanning microscope (CLSM), nitrogen adsorption experiments, and temperature-programmed heating experiments, this study examined the surface morphologies, pore structures, and oxidation characteristic parameters of coals following sample processing using low-temperature nitrogen. …”
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Augmenter of Liver Regeneration Crotonylation Assists in Mitochondria-ER Contact to Alleviate Hepatic SteatosisSummary by Xiao-lin Wang, Jia-hao He, Ping Xie, Yuan Wu, Ling-yue Dong, Wei An

“…However, the connection between dysregulated ALR crotonylation and MASLD pathogenesis remains largely unknown. Methods: Steatotic liver samples from human and Western diet (WD)-fed mice were employed for detecting Kcr levels. …”
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Transcription Analysis of the THBS2 Gene through Regulation by Potential Noncoding Diagnostic Biomarkers and Oncogenes of Gastric Cancer in the ECM-Receptor Interaction Signaling P... by Ali Barani, Kamyar Beikverdi, Benyamin Mashhadi, Naeimeh Parsapour, Mohammad Rezaei, Pegah Javid, Mansoureh Azadeh

“…Validation of expression and coexpression analyses was performed using a qRT-PCR experiment. Results. Based on bioinformatics analyses, THBS2 (FC: 7.14, FDR<0.0001) has a significantly high expression in GC samples. lncRNAs BAIAP2-AS1, TSIX, and LINC01215 have RNA interaction with THBS2. …”
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Anti-Inflammatory Effects of SGLT1 Synthetic Ligand in In Vitro and In Vivo Models of Lung Diseases by Cristiano Rumio, Giuseppina Dusio, Diego Cardani, Barbara La Ferla, Giuseppe D’Orazio

“…In this study, we report the anti-inflammatory effects observed in the lung upon engagement of the transporter, and upon the use of glucose and BLF501, a synthetic SGLT1 ligand, for the treatment of animal models of lung inflammation, including a model of allergic asthma. <b>Methods.</b> In vitro experiments were carried out on human pneumocytes stimulated with LPS from <i>Pseudomonas aeruginosa</i> and co-treated with glucose or BLF501, and the production of IL-8 was determined. …”
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NAT10 promotes gastric cancer progression by enhancing the N4-acetylcytidine modification of TNC mRNA by Yu Chen, Jinzhou Wang, Jiuhua Xu, Ruilong Kou, Bin Lan, Zhiwei Qin

“…This study aimed to explore the potential mechanism by which NAT10 regulated GC progression. Methods Clinical samples were used to study the correlation between NAT10 expression and poor prognosis in patients with GC by univariate analysis and multivariate analysis. …”
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Rbms3 Upregulation in Mesenchymal Stem Cells Impairs Fracture Healing in Type 2 Diabetes Mellitus by Liu C, Yu Y, Tian L, Liu Y, Zhang D, Yu A

“…Further validation and exploration were carried out through in vitro experiments (cell transduction, flow cytometry, ALP staining, ARS, qPCR, and Western blotting) and in vivo experiments (micro-CT, histological staining, and immunohistochemistry).Results: Our study identified differentially expressed genes from fracture healing and non-union cases in human samples, suggesting abnormal immune infiltration and disrupted biological processes. …”
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Gastrin-releasing peptide receptor: a promising new biomarker to identify cervical precursor lesions and cancer by Martina Lichtenfels, Rafaella Almeida Lima Nunes, Rossana Veronica Mendoza López, Camila Alves da Silva, Luiz Carlos Zeferino, Vanesca de Souza Lino, Adhemar Longatto-Filho, Louise De Brot, Silvia Helena Rabelo-Santos, Daniela Baumann Cornelio, Enrique Boccardo, Caroline Brunetto de Farias, Lara Termini

“…Generic primers (GP5+/GP6+) were used to identify HPV infection in tissue samples. Experiments involving cell lines were analyzed through non-parametric tests (Kruskal Wallis), and Fisher's Exact Test for human tissues samples. …”
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Spatial Transcriptomic Signatures of Early Acute T Cell–mediated Rejection in Kidney Transplants by Minji Kang, BS, Haeyoon Kwon, Jeongin Song, BS, Yunyoung Jang, BS, Seung Hee Yang, PhD, Seung-Min Cha, BS, Ji Hwan Moon, PhD, Yong Chul Kim, MD, PhD, Hyun Je Kim, MD, PhD

“…Despite an emphasis on suppressing T cell–mediated immune responses, successful control over TCMR is not always achieved, suggesting the potential involvement of factors beyond T cells. Methods. Biopsy samples from suspicious (borderline) for acute TCMR (borderline TCMR) and non-TCMR patients were obtained 9 d postsurgery, and spatial transcriptomics profiling was conducted using the GeoMx Digital Spatial Profiler platform. …”
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Molecular techniques for the assessment of Cr (VI) reduction by Bacillus thuringiensis by Deisy L. Guerrero Ceballos, Eduardo Ibargüen Mondragón, Pablo Fernández Izquierdo, Jhonatan Pinta Melo, Edith Mariela Burbano Rosero

“…Samples of 20 mL were taken every 12 h from each treatment to track Cr (VI) reduction levels and to confirm microorganism identity via molecular methods involving denaturing gradient gel electrophoresis (DGGE), restriction enzyme digestion profiles (RFLP), and bioinformatic analysis. …”
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Inhibition of id-1 reduces osteosarcoma growth and metastasis through mediation of snail by Rongbing Shu, Zhuanyi Yu, Jianmin Wu, Qiuxin Cheng, Zhihao Peng, Huaqiang Zhou, Min Zhao

“…This study aims to investigate the role of the Id-1 gene in OS invasion and metastasis, and its relationship with the Snail gene. Methods This study included tissue samples from 12 non-metastatic osteosarcomas and 9 metastatic osteosarcoma patients to examine the expression of Id-1 and Snail using RT-qPCR and analyze their correlation. …”
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Precision computerised cognitive behavioural therapy (cCBT) intervention for adolescents with depression (SPARX-UK): protocol for the process evaluation of a pilot randomised contr... by Charlotte Lucy Hall, Chris Hollis, Kareem Khan, Peter Fonagy, Kirsty Sprange, Cathy Creswell, Yi Yang, Vibhore Prasad, Kapil Sayal, Nitish Jawahar, Ellen Townsend, Dorothee Auer, Praveetha Patalay, Aja Murray, Yvonne Kelly, Boliang Guo, Christopher R Tench, Paul Stallard, Louise Arseneault, Sieun Lee, Sally Merry, Charlotte Hall, Jo Gregory, Rory O’Connor, Emma Nielsen, Edmund Sonuga-Barke, Hannah Wright, Petr Slovak, Emily Lloyd, Josimar De Alcantara Mendes, Carolyn Ten Holter, Marina Jirotka, Aaron Kandola, Sonia Livingstone, Kasia Kostryka-Allchorne, Jake Bourgaize, Mariya Stoilova, Marianne Etherson, Chris Greenhalgh, Jim Warren, Vajisha Wanniarachchi, Kevin Glover, Mathijs Lucassen, Karolina Stasiak, Camilla Babbage, Adam Parker, Joanna Lockwood, Elvira Perez Vallejos, Rebecca Woodcock, Sarah Doherty, Lucy-Paige Willingham, Camilla May Babbage, Matthew Jeffery, Peiyao Tang, Holly Griffiths, Lily Roberts, A. Jess Williams, Lucy Hitcham

“…This protocol paper describes the rationale, aims and methodology of the SPARX-UK trial process evaluation.Methods and analysis The process evaluation will use a mixed-methods design following the UK Medical Research Council’s 2015 guidelines, comprising quantitative and qualitative data collection. …”
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The Influence of Mixing Orders on the Microstructure of Artificially Prepared Sand-Clay Mixtures by Kexin Yin, Anne-Laure Fauchille, Eugenia Di Filippo, Khaoula Othmani, Samuel Branchu, Giulio Sciarra, Panagiotis Kotronis

“…The following methods were used to validate this statement: (1) optical observation, (2) X-ray tomography, (3) scanning electron microscopy, and (4) Mercury intrusion porosimetry. …”
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Characteristics of interpenetrating hydrogels based on fibrin and high molecular weight polyvinyl alcohol by V. G. Matveeva, M. A. Rezvova, T. V. Glushkova, E. A. Senokosova, M. Yu. Khanova, E. O. Krivkina, E. A. Torgunakova, L. V. Antonova

“…Aim. In an in vitro experiment, to study the properties of the IPN hydrogel fibrin/high-molecular polyvinyl alcohol with an increased number of cryocycles and fibrinogen concentrations to assess the prospects for its use in the creation of small-diameter vascular prostheses.Materials and Methods. …”
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Volumetric atlas of the rat inner ear from microCT and iDISCO+ cleared temporal bones by Daniele Cossellu, Elisa Vivado, Laura Batti, Ivana Gantar, Roberto Pizzala, Paola Perin

“…In the present work, we segmented and annotated 34 structures in the rat inner ear, yielding a detailed volumetric inner ear atlas which can be integrated with the Waxholm rat brain atlas. Methods We performed iodine-enhanced microCT and iDISCO+-based clearing and fluorescence lightsheet microscopy imaging on a sample of rat temporal bones. …”
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Review of machine learning applications for predicting the quality of biomass briquettes for sustainable and low-carbon energy solutions by Constance Nakato Nakimuli, Fred Kaggwa, Johan De Greef, David Kilama Okot, Julien Blondeau, Simon Kawuma

“…This review discusses how Machine Learning has been applied to predict the quality of biomass briquettes produced from agricultural and municipal solid organic waste, which are crucial for advancing green and low-carbon energy solutions. Traditional methods of assessment of briquette quality involve destructive laboratory experiments, do not favor sample reuse, are time-consuming, and labor-intensive, posing barriers to efficient production. …”
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Comprehensive analysis of disulfidptosis-related genes and the immune microenvironment in heart failure by Linna Zhao, Juanjuan Zhang, Juanjuan Zhang, Qiuhang Song, Cheng Dai, Yiping Qin, Aiying Li, Aiying Li

“…The effect of these DRGs on the characteristics of the immune microenvironment was further explored, such as infiltrating immune cells, immune response gene sets, and HLAs genes.ResultsAll four DRGs were dysregulated in HF samples. The predictive model based on these four DRGs effectively differentiated between HF patients and healthy individuals, which was validated in the experiment. …”
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Cyclin F Expression in Clear Cell Renal Cell Carcinoma and Its Effect on Biological Behavior of Renal Carcinoma Cell Lines by Min SU, Yan WANG, Jie HUA, Tianyun WANG, Shengnan XU, Xiang KUI

“…ObjectiveTo investigate the expression of Cyclin F in clear cell renal cell carcinoma (ccRCC), its clinicopathological characteristics, and its effect on the biological behavior of renal cancer cell lines MethodsRT-qPCR and Western blot were used to detect the mRNA and protein expression of Cyclin F in fresh ccRCC specimens. …”
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