The Construction of a Standard Karyotype of Intermediate Wheatgrass and Its Potential Progenitor Species by Lin Wang, Shuang Liang, Fei Qi, Yinguang Bao, Richard R.-C. Wang, Xingfeng Li

“…FISH karyotype was established using pSc119.2-1, (GAA)₁₀, AFA-3, AFA-4, pAs1-1, pAs1-3, pAs1-4, and pAs1-6 as a combined multiplex oligonucleotide probe. …”
Get full text

The haemagglutinin gene of bovine-origin H5N1 influenza viruses currently retains receptor-binding and pH-fusion characteristics of avian host phenotype by Jiayun Yang, Mehnaz Qureshi, Reddy Kolli, Thomas P. Peacock, Jean-Remy Sadeyen, Toby Carter, Samuel Richardson, Rebecca Daines, Wendy S. Barclay, Ian H. Brown, Munir Iqbal

“…Clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus (HPAIV) has caused a panzootic affecting all continents except Australia, expanding its host range to several mammalian species. …”
Get full text

Geochemical characteristics of hot spring fluids in the Xunwu-Shicheng fault zone of southern Jiangxi by Fuqiang XIAO, Yongjun ZOU, Shuanglong ZHANG, Xing QI, Weidong XIAO

“…The results show that the hydrochemical types of hot springs in the Xunwu-Shicheng fault zone are mainly HCO3•SO4—Na•Ca and HCO3—Na. The hot springs are formed by the heating of precipitation infiltrating deep thermal storage structures along fault zones. …”
Get full text

Enhanced Natamycin production in Streptomyces gilvosporeus through phosphate tolerance screening and transcriptome-based analysis of high-yielding mechanisms by Liang Wang, Wen Xiao, Ting Qiu, Hongjian Zhang, Jianhua Zhang, Xusheng Chen

“…Due to the complexity of cellular metabolism, evolutionary engineering is required for developing strains with enhanced natamycin biosynthetic capacity. …”
Get full text

Quantitative analysis of the flight calls and trills of Evening Grosbeaks (Coccothraustes vespertinus) by William M. Kirsch, Caleb T. Centanni, Song Han Ngo, Matthew A. Young, Thomas P. Hahn, W. Douglas Robinson

“…Flight calls of types 1, 2, 3, and 4 were readily distinguishable from each other but trills were less distinctive. …”
Get full text

South Asia as a Reservoir for the Global Spread of Ciprofloxacin-Resistant Shigella sonnei: A Cross-Sectional Study. by Hao Chung The, Maia A Rabaa, Duy Pham Thanh, Niall De Lappe, Martin Cormican, Mary Valcanis, Benjamin P Howden, Sonam Wangchuk, Ladaporn Bodhidatta, Carl J Mason, To Nguyen Thi Nguyen, Duong Vu Thuy, Corinne N Thompson, Nguyen Phu Huong Lan, Phat Voong Vinh, Tuyen Ha Thanh, Paul Turner, Poda Sar, Guy Thwaites, Nicholas R Thomson, Kathryn E Holt, Stephen Baker

“…We reconstructed the recent evolutionary history of these organisms and combined these data with their geographical location of isolation. …”
Get full text

Genome-wide association studies unveil major genetic loci driving insecticide resistance in Anopheles funestus in four eco-geographical settings across Cameroon by Mahamat Gadji, Jonas A. Kengne-Ouafo, Magellan Tchouakui, Murielle J. Wondji, Leon M. J. Mugenzi, Jack Hearn, Onana Boyomo, Charles S. Wondji

“…Abstract Background Insecticide resistance is jeopardising malaria control efforts in Africa. Deciphering the evolutionary dynamics of mosquito populations country-wide is essential for designing effective and sustainable national and subnational tailored strategies to accelerate malaria elimination efforts. …”
Get full text

Comparative genomic characterization of Francisella tularensis strains belonging to low and high virulence subspecies. by Mia D Champion, Qiandong Zeng, Eli B Nix, Francis E Nano, Paul Keim, Chinnappa D Kodira, Mark Borowsky, Sarah Young, Michael Koehrsen, Reinhard Engels, Matthew Pearson, Clint Howarth, Lisa Larson, Jared White, Lucia Alvarado, Mats Forsman, Scott W Bearden, Anders Sjöstedt, Richard Titball, Stephen L Michell, Bruce Birren, James Galagan

“…We have also identified genes exhibiting sequence similarity to effectors of the Type III (T3SS) and components of the Type IV secretion systems (T4SS). …”
Get full text

Exploring phonon mediated superconductivity of $$\hbox {LiB}_{{2}} \hbox {N}_{{2}}$$ and $$\hbox {LiC}_{{2}} \hbox {N}_{{2}}$$ under high pressure insight from first-principles cal... by Prutthipong Tsuppayakorn-aek, Thiti Bovornratanaraks, Komsilp Kotmool

“…In this study, we examine $$\hbox {LiB}_{{2}} \hbox {N}_{{2}}$$ and $$\hbox {LiC}_{{2}} \hbox {N}_{{2}}$$ , materials identified through an evolutionary algorithm, which exhibit thermodynamic stability up to at least 100 GPa. …”
Get full text

Comorbidades do transtorno de déficit de atenção e hiperatividade em crianças escolares Attention-deficit hyperactivity disorder comorbidity in a school sample of children by Marianne de Aguiar Possa, Lucas Spanemberg, Ana Guardiola

“…Onze das quinze crianças (73,3%) sem comorbidades eram do tipo desatentas ou hiperativas (p<0,05). …”
Get full text

Updating the Relationship Between the Threshold Value of Average Nucleotide Identity and Digital DNA–DNA Hybridization for Reliable Taxonomy of <i>Corynebacterium</i> by Haitham Elbir

“…</i> The strains 335C, 1103A, 2571A, and 2298A were classified as novel <i>Corynebacterium</i> based on the ANI value (77.12, 94.01%, 94.26%, and 94.03%) and dDDH value (21.3%, 54.1%, 54.9%, and 51.3%), respectively. Genes for menaquinone biosynthesis and the saturation of chains were detected in uterine strains and their closely related type strains. …”
Get full text

Screening and comparison of in vitro-induced resistant and clinically resistant Stenotrophomonas maltophilia strains for eravacycline resistance-related genes by Jie Wu, Jiarong Song, Junchang Cui

“…This work provides the evidence for CNV as a core resistance driver and highlights evolutionary divergence under clinical pressure, informing future antibiotic development and resistance surveillance strategies.…”
Get full text

Leveraging AI to explore structural contexts of post-translational modifications in drug binding by Kirill E. Medvedev, R. Dustin Schaeffer, Nick V. Grishin

“…Scientific contribution: Using recent AI-based approaches for protein structure prediction (AlphaFold3, RoseTTAFold All-Atom, Chai-1), we generated 14,178 models of PTM-modified human proteins with docked ligands. …”
Get full text

Evolution of temporomandibular joint reconstruction: from autologous tissue transplantation to alloplastic joint replacement by Hanghang Liu, Liwei Huang, Shibo Liu, Linyi Liu, Bolun Li, Zizhuo Zheng, Yao Liu, Xian Liu, En Luo

“…It delves into the differences of each surgical technique and outlines the implications of recent technological advances, such as 3D printing, which hold the promise of enhancing surgical precision and patient outcomes. …”
Get full text

Exclusive breastfeeding is associated with the gut microbiome maturation in infants according to delivery mode by Nathalia F. Naspolini, Paulo A. Schüroff, Pedro A. R. Vanzele, Davi Pereira-Santos, Tamires Amabili Valim, Kevin S. Bonham, André Fujita, Maria Rita Passos-Bueno, Patricia C. B. Beltrão-Braga, André C. P. L. F. Carvalho, Vanja Klepac-Ceraj, Guilherme V. Polanczyk, Alline C. Campos, Carla R. Taddei

Get full text

Chromosome-level genome assembly and annotation of largemouth bronze gudgeon (Coreius guichenoti) by Xuemei Li, Xingbing Wu, Yongjiu Zhu, Xiaoli Li, Zihao Meng, Nian Wei, Miao Xiang, Deguo Yang, Tingbing Zhu

“…Moreover, a total of 22,506 protein-coding genes were predicted with average intron length of 2293 bp. Evolutionary analysis and divergence time prediction revealed that C. guichenoti was closely related to C. heterodon and they phylogenetically diverged from common ancestor ~20.7 million years ago (Mya), following the separation of Cyprinidae at 28.3 Mya. …”
Get full text

Discovery of CRISPR-Cas12a clades using a large language model by Yuanyuan Feng, Junchao Shi, Zhanwei Li, Yongqian Li, Jiaxi Yang, Shisheng Huang, Jinfang Zheng, Wei Han, Yunbo Qiao, Jun Zhang, Qi Liu, Yao Yang, Chunyi Hu, Lina Wu, Xiaokang Zhang, Jin Tang, Xingxu Huang, Peixiang Ma

“…In this work, we employ an evolutionary scale language model (ESM) to learn the information beyond sequences. …”
Get full text

A nearly gapless, highly contiguous reference genome for a doubled haploid line of Populus ussuriensis, enabling advanced genomic studies by Wenxuan Liu, Caixia Liu, Song Chen, Meng Wang, Xinyu Wang, Yue Yu, Ronald R. Sederoff, Hairong Wei, Xiangling You, Guanzheng Qu, Su Chen

“…This T2T P. ussuriensis reference genome will not only enhance our understanding of genome structure, and functions within the poplar genus but also provides valuable resources for poplar genomic and evolutionary studies.…”
Get full text

Transcriptomic Analysis of Venom Secretion in <i>Achelura yunnanensis</i>: Lipid Metabolism, Redox Reactions, and Structural Adaptations by Ping Liu, Hui-Qin Zhu, Si-Ming Wang, Yu-Qian Wang, Zhen-Yuan Ruan, Lu Qiao, Xing-Xing Wu, Qing-Hua Yan, Ya-Ping Lu, Bing Bai, Wei-Feng Ding

“…These findings reveal a multi-component mechanism in LDET that supports venom secretion through metabolic and structural adaptations, with lipid metabolism genes constituting 18.3% of total differentially expressed genes, highlighting evolutionary trade-offs in insect defense. …”
Get full text

Investigation of Avian Reovirus Evolution and Cross-Species Transmission in Turkey Hosts by Segment-Based Temporal Analysis by Cheng-Shun Hsueh, Michael Zeller, Amro Hashish, Olufemi Fasina, Pablo Piñeyro, Ganwu Li, Jianqiang Zhang, Mohamed El-Gazzar, Yuko Sato

“…Despite its ubiquity, the evolutionary history of ARV cross-species transmission among chickens, turkeys, and wild birds remains poorly understood, hindering effective control and surveillance. …”
Get full text