In vitro methodologies to evaluate nanocarriers for cancer treatment: where are we? by Giovanna Cassone Salata, Camila Megumi Hirokawa, Giovanna Barros de Melo, Isabella Draszesski Malagó, Jessica Ribeiro Nunes, Julia Sapienza Passos, Regina Gomes Daré, Luciana B. Lopes

“…Abstract Cancer treatment continues to face significant challenges, including lack of selectivity, systemic toxicity, and the adaptive resistance mechanisms of cancer cells, which limit the effectiveness of conventional therapies. Nanomedicine offers a promising solution, using nanocarriers for targeted drug delivery, improved bioavailability, and reduced off-target effects due to properties such as the enhanced permeability and retention (EPR) effect and advanced surface modifications. …”
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GPX3 promotes cisplatin resistance in TNBC by manipulating ROS-TGFB1-ZEB2 by Qingyi Hu, Qianzhi Chen, Wen Yang, Anwen Ren, Jie Tan, Tao Huang

“…The correlation between the expression level of GPX3, TGFB1, and ZEB2 was analyzed using breast cancer microarrays and the TCGA database. The effect of GPX3 on platinum sensitivity in TNBC was studied using a mouse xenograft model. …”
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Phosphoribosyl transferase domain containing 1: A prognostic biomarker in testicular germ cell tumors by Peisheng Huang, Yihao Chen, Yongcheng Shi, Chuanfan Zhong, Huawei Lin, Xiaoxue Yu, Kai Chen, Zhuoya Huang, Le Zhang, Shumin Fang, Jianming Lu, Jiahong Chen

“…Immunohistochemistry on tissue microarrays validated PRTFDC1’s predictive value in clinical samples. …”
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Targeting Mesothelin Enhances Personalized Neoantigen Vaccine Induced Antitumor Immune Response in Orthotopic Pancreatic Cancer Mouse Models by Zhixiong Cai, Zhenli Li, Wenting Zhong, Fangzhou Lin, Xiuqing Dong, Honghao Ye, Yutong Guo, Geng Chen, Xiaoling Yu, Haijun Yu, Ruijing Tang, Xiaolong Liu

“…Abstract The immunosuppressive microenvironment in pancreatic cancer, characterized by low tumor‐specific T cells and excessive fibrosis, limits the effectiveness of immunotherapy. Here, three datasets and multi‐immunofluorescence staining of tissue microarrays in pancreatic cancer indicate that mesothelin (MSLN) expression negatively correlates with cytotoxic T cells in tumor. …”
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Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1 by Yijian Deng, Liying Zeng, Huaxi Liu, Anna Zuo, Jie Zhou, Ying Yang, Yanting You, Xinghong Zhou, Baizhao Peng, Hanqi Lu, Shuai Ji, Ming Wang, Yigui Lai, Hiu Yee Kwan, Xiaomin Sun, Qi Wang, Xiaoshan Zhao

“…Finally, the inhibitory effects of silibinin on ferroptosis were reversed by knocking down FTH1 in vitro. …”
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Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1 by Guoqing Su, Jinghang Qian, Yi Wang, Yu Zhu, Yanyan Chen, Jingru Shen, Jiayuan Jiang, Yuepeng Cao, Nannan Wang, Xing Huang, Chengshuai Si, Xu Zhang, Peng Shao, Yongxia Ye, Yang Wang, Jun Bao, Liu Yang

“…Analysis of CRC tissue microarrays and TCGA-CRC patient data indicated that LINC00183 is often overexpressed in CRC, in association with advanced tumor stage and poor survival. …”
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Applicability of epigenetic age models to next-generation methylation arrays by Leonardo D. Garma, Miguel Quintela-Fandino

“…As new methylation microarrays are developed and older models discontinued, existing epigenetic clocks might become obsolete. …”
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NONO regulates m5C modification and alternative splicing of PTEN mRNAs to drive gastric cancer progression by Gaichao Zhao, Ruochen liu, Lingjun Ge, Dan Qi, Qishu Wu, Zini Lin, Houji Song, Liping Zhong, Hongjuan Cui

“…Abstract Background The effect of m5C modification on oncogene mRNAs has been well studied, while little is known about its influence on mRNAs of tumor suppressor genes (TSGs). …”
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Alliin, a Garlic (Allium sativum) Compound, Prevents LPS-Induced Inflammation in 3T3-L1 Adipocytes by Saray Quintero-Fabián, Daniel Ortuño-Sahagún, Manuel Vázquez-Carrera, Rocío Ivette López-Roa

“…However its potential anti-inflammatory effect is unknown. We examined the effects of alliin in lipopolysaccharide- (LPS-) stimulated 3T3-L1 adipocytes by RT-PCR, Western blot, and microarrays analysis of 22,000 genes. …”
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Genetic Dissection of Learning and Memory in Mice by Yann S. Mineur, Wim E. Crusio, Frans Sluyter

“…Next, we briefly review more recently developed techniques, such as microarrays and RNA interference. In addition, we focus on gene-environment interactions and endophenotypes. …”
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Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish. by Gustavo Gomez, Jae-Hyung Lee, Matthew B Veldman, Jing Lu, Xinshu Xiao, Shuo Lin

“…Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. …”
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Dual-targeting and steric hindrance resolution in HER2 IHC: a novel approach to improve diagnostic sensitivity by Li Luo, Xi Zhang, Linqiong Chen, Zhuohan Chen, Yuchen Wang, Kaihao Huang, Xiaoyun Lin, Hongxiang Zhu, Wangqi Du

“…Additionally, significantly higher HER2 scores were observed in most BC tissues on tissue microarrays (TMAs) compared to those diagnosed using the traditional method. …”
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p53 activation by knockdown technologies. by Mara E Robu, Jon D Larson, Aidas Nasevicius, Soraya Beiraghi, Charles Brenner, Steven A Farber, Stephen C Ekker

“…Interestingly, quantitative reverse-transcriptase PCR, microarrays and whole-mount in situ hybridization assays show that MO off-targeting effects are accompanied by diagnostic transcription of an N-terminal truncated p53 isoform that uses a recently recognized internal p53 promoter. …”
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Electrochemical Strategies for MicroRNA Quantification Leveraging Amplification and Nanomaterials: A Review by Alexander Hunt, Gymama Slaughter

“…However, conventional detection methods, such as Northern blotting, RT-PCR, and microarrays, are limited by low sensitivity, lengthy protocols, and limited specificity. …”
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Inhibition of PDK modulates radiotherapy resistance in gastric cancer by Qi Zhang, Wanjia Qiao, Xiaoyu Liu, Jun Lu, Farooq Benish, Shuping Li, Xiaojun Liu

“…Abstract To explore the effect of sodium dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, on the progression of gastric cancer and resistance to radiotherapy, we analyzed histopathological microarrays from 60 gastric cancer and paracancerous tissues to determine PDK expression and its prognostic significance. 5-Ethynyl-2′-deoxyuridine (EdU) incorporation assay and Transwell migration assay were used to investigate the effects of PDK inhibition on gastric cancer cell proliferation and migration. …”
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The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment by Ågot Aakra, Heidi Vebø, Ulf Indahl, Lars Snipen, Øystein Gjerstad, Merete Lunde, Ingolf F. Nes

“…Efflux of drug out of the cells may be one mechanism used by V583 to overcome the effect of chloramphenicol.…”
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Comprehensive analysis of single-cell and bulk RNA sequencing data unveils antigen-presenting and processing fibroblasts and establishes a predictive model in gastric cancer by Chenggang Zhang, Fangqi Chen, Jie Li, Yixuan He, Juan Sun, Zicheng Zheng, Guanmo Liu, Yihua Wang, Weiming Kang, Xin Ye

“…The predictive model based on APPFRGs effectively predicts the prognosis and tumor immune status of GC.…”
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Adverse predictive value of ASPM on lung adenocarcinoma overall survival depended on chemotherapy status by Yan Zheng, Baichen Sun, Zhiling Qu

“…This study investigates the expression and prognostic value of ASPM, focusing on its role in chemotherapy outcomes.Methods We analyzed the prognostic relevance of ASPM using bioinformatics, immunohistochemical staining of LUAD tissue microarrays, and proteomics data. Further, in vitro experiments were conducted to evaluate the effects of ASPM overexpression on cell proliferation and sensitivity to cisplatin.Results Bioinformatics analysis revealed that ASPM’s prognostic significance differed between transcriptomic and proteomic datasets. …”
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MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis by Yi Xie, Congyu Xue, Shuai Guo, Lei Yang

“…This research focused on the effects of miR-520a on the development of non-small-cell lung cancer (NSCLC) and the molecules involved. …”
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Endoplasmic reticulum protein ERp46 in renal cell carcinoma. by Wilhelmina C M Duivenvoorden, Athanasios Paschos, Sarah N Hopmans, Richard C Austin, Jehonathan H Pinthus

“…The objectives of this study were to investigate ERp46 in RCC, its expression, its effects on RCC growth in a mouse model and whether it interacts with AdipoR1. …”
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