Decoding chromosomal instability insights in CRC by integrating omics and patient-derived organoids by Federica Papaccio, Manuel Cabeza-Segura, Blanca García-Micó, Francisco Gimeno-Valiente, Sheila Zúñiga-Trejos, Valentina Gambardella, María Fernanda Gutiérrez‐Bravo, Carolina Martinez‐Ciarpaglini, Pilar Rentero‐Garrido, Tania Fleitas, Susana Roselló, Juan Antonio Carbonell-Asins, Marisol Huerta, David Moro-Valdezate, Desamparados Roda, Noelia Tarazona, Manuel M. Sánchez del Pino, Andrés Cervantes, Josefa Castillo

“…From a clinical perspective, a better knowledge of these tumour’s biology will help to guide therapeutic strategies more effectively. Methods We used high-density chromosomal microarray analysis to evaluate CIN level of patient-derived organoids (PDOs) and their original mCRC tissues. …”
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Variations in salivary microbiome and metabolites are associated with immunotherapy efficacy in patients with advanced NSCLC by DanHui Huang, YueHua Chen, Cui Li, Shuang Yang, LiShan Lin, XiaoNan Zhang, XiaoFang Su, LaiYu Liu, Haijin Zhao, Tingyue Luo, Shaoxi Cai, QianNan Ren, Hangming Dong

“…Besides, in vitro experiments and tumor tissue microarray, including 70 NSCLC patients, were utilized to further explore the potential mechanism linking the oral microbiome and immunotherapy efficacy. …”
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LAPTM4B enhances the stemness of CD133+ liver cancer stem-like cells via WNT/β-catenin signaling by Jiahong Wang, Jianping Liao, Ye Cheng, Meirong Chen, Aimin Huang

“…Therefore, we investigated the effects of LAPTM4B on the stemness regulation of cluster of differentiation 133 (CD133)+ liver cancer stem-like cells (CSLCs). …”
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Aldo-keto reductase family 1 member B10 prevents esophageal squamous cell carcinoma from reactive carbonyl species-induced cell death and promotes its progression by Shau-Hsuan Li, Wan-Ting Huang, Yen-Hao Chen, Hung-I Lu, Chien-Ming Lo, Hsin-Ting Tsai, Chang-Han Chen

“…Furthermore, the identified signaling pathway and its biological effects were investigated in ESCC cell lines in vitro and 4-nitroquinoline 1-oxide (4-NQO)-induced-ESCC murine model in vivo. …”
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Midline 1 associated with Fas signaling enhances murine antigen-induced arthritis by Nina Lukač, Nina Lukač, Darja Flegar, Darja Flegar, Sara Priselac, Tomislav Kelava, Tomislav Kelava, Alan Šućur, Alan Šućur, Maša Filipović, Maša Filipović, Dino Šisl, Dino Šisl, Martina Fadljević, Igor Radanović, Vedran Katavić, Vedran Katavić, Nives Zimmermann, Danka Grčević, Danka Grčević, Nataša Kovačić, Nataša Kovačić

“…Transcriptome of sorted CD11b+Gr-1+ cells was analyzed by microarray, and effects of potential molecular driver Midline-1 (Mid-1) were analyzed in vitro and using Mid1 −/− mice.ResultsAmeliorated antigen-induced arthritis in Fas −/− mice is characterized by the lack of synovial accumulation of myeloid CD11b+Gr-1+ cells. …”
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Cellular interactions and Ion channel signatures in atrial fibrillation remodeling: insights from single-cell analysis and machine learning by Bin He, Yan Cheng, Juan Wang, Ya Zhan, YanQun Liu

“…Ion channel-related genes were extracted from microarray datasets and analyzed for differential expression and functional relevance to AF pathology. …”
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Metabolic‐Immune Suppression Mediated by the SIRT1‐CX3CL1 Axis Induces Functional Enhancement of Regulatory T Cells in Colorectal Carcinoma by Ruiyang Zi, Xiang Zhao, Limei Liu, Yijie Wang, Rui Zhang, Zhiheng Bian, Haoran Jiang, Taorui Liu, Yixin Sun, Han Peng, Xuesong Wang, Fanghao Lu, Chao Zhang, Fan Zhang, Qing Qin, Houjie Liang, Jianjun Li, Zhihao Wei, Yan Dong

“…Multiplex immunofluorescence (mIHC) analysis of a CRC tissue microarray confirmed the promoting effect of CX3CL1 on Treg infiltration. …”
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Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis by Chang Liu, Lei Shi, Zijun Meng, Manlin Zhang, Zhiqi Zhang, Yunzhe Li, Kaiwen Du, Muyao Yang, Lin Qiu, Jing Feng, Yuchen He, Jiayun Liu, Hua Zhang, Hongbin Zhang, Tingyuan Lang, Zhuo Yang

“…Combined tandem mass tag- and tissue microarray-based proteomic studies revealed YWHAB down-regulation in post-neoadjuvant chemotherapy OCPM tissues, which was confirmed in no-neoadjuvant-chemotherapy-response tissues, isolated OCPM stem cells, and induced cisplatin-resistant cells. …”
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Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5’tRFAla/DVL/β-catenin pathway inhibition by Zhipeng Zhang, Yanmei Wu, Wenqiang Liang, Zhifang Liao, Hongbo Liao, Xingxing Xing, Wenxin Yi, Zixuan Liu, Yicheng Li, Mengya Shi, Dongling Lin, Ting Gu, Biao Wu, Mingzhi Zou, Huilai Miao, Xin Wu

“…Abstract Although tsRNA has been demonstrated to modulate various physiological processes analogous to miRNA, the potential regulatory functions and mechanisms of tsRNAs related to the pharmacological effects of small molecule drugs remain unclear. Herein, it is shown that eurycomalactone (ELT), a natural product, can reversibly switch hepatocellular carcinoma (HCC) PLC/PRF/5 and HUH7 cells into a quiescent state. …”
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Interferon-stimulated circHOMER1 attenuates antiviral innate immunity by Ao Zhang, Ranran Yao, Shijin Geng, Haiying Wang, Rong-Chun Tang, Hengxiang Yu, Rui Xu, Hong-Yan Chen, Yunxuan Zhou, Lan Zhang, Jun Zhang

“…Notably, the concordant immunosuppressive effects of circHOMER1 were observed in mouse infection models. …”
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Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson’s disease progression by targeting DCLK1 by Li Liu, Xiangting Liu, Runze Zhang, Xiao Sun, Keping Zhang, Beibei Zheng, Ruoxi Yang, Kaiyue Yang, Guohua Song, Zhaoqiang Zhang

“…Levels of miRNA in exos were analyzed by gene expression profile microarray. Levels of miR-150-5p in exos and substantia nigra pars compacta (SNpc) were further confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
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E2F2(E2F transcription factor 2) as a potential therapeutic target in meibomian gland carcinoma: evidence from functional and epigenetic studies by Wei Wang, Hetong Wang, Xun Liu, Fei Xu, Qin Tang, Chuanli Zhang, Jiaqi Lin, Limin Zhu, Tingting Lin

“…Tissue samples from 3 normal meibomian glands (MG) and 36 MGC patients were used to construct a tissue microarray. Functional assays were performed by modifying E2F2 expression, including CCK8, wound healing, Transwell, and analysis of epithelial-mesenchymal transition (EMT)-related markers. …”
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Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy by Li-Min Lei, Fu-Xing-Zi Li, Xiao Lin, Feng Xu, Su-Kang Shan, Bei Guo, Ming-Hui Zheng, Ke-Xin Tang, Yi Wang, Qiu-Shuang Xu, Wen-Lu Ouyang, Jia-Yue Duan, Yun-Yun Wu, Ye-Chi Cao, Zhi-Ang Zhou, Si-Yang He, Yan-Lin Wu, Xi Chen, Zheng-Jun Lin, Yi Pan, Ling-Qing Yuan, Zhi-Hong Li

“…This study identifies that CT-EXO mediates CT-induced osteoporotic effects through miR-25-3p by inhibiting autophagy via targeting SATB2, presenting a novel mechanism underlying the effect of cold temperature on bone mass.…”
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GDF11 protects against mitochondrial-dysfunction-dependent NLRP3 inflammasome activation to attenuate osteoarthritis by Pengfei Zhang, Haoxin Zhai, Shuai Zhang, Xiaojie Ma, Ao Gong, Zhaoning Xu, Wei Zhao, Hui Song, Shufeng Li, Tengfei Zheng, Zhendong Ying, Lei Cheng, Yunpeng Zhao, Lei Zhang

“…In an in vivo surgically induced mouse model, intraarticular administration of recombinant human GDF11 alleviated OA pathogenesis, whereas GDF11 conditional knockout reversed this effect. Additionally, findings from the NLRP3-knockout DMM mouse model revealed that GDF11 exerted its protective effect by inhibiting NLRP3. …”
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Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling by Jiachen Dong, Jiachen Dong, Jiachen Dong, Yue Liao, Yue Liao, Yue Liao, Mengjun Sun, Mengjun Sun, Mengjun Sun, Huiwen Chen, Huiwen Chen, Huiwen Chen, Kecong Zhou, Kecong Zhou, Kecong Zhou, Huanyu Zhang, Huanyu Zhang, Huanyu Zhang, Wei Zhou, Wei Zhou, Wei Zhou, Zhongchen Song, Zhongchen Song, Zhongchen Song

“…The effects of gingipains on bone marrow mesenchymal stem cells (BMSCs) and RAW264.7 macrophages were assessed using cell proliferation assays, qPCR, western blot, microarray analysis, and dual-luciferase reporter assays. …”
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MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
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Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation by Jiahui Dong, Ruiping Du, Yani Zhang, Bo Hong, Gengyun Sun

“…Cell apoptosis and cell cycle were detected by flow cytometry. The effect of CAV1 on EGFR degradation was verified by cycloheximide inhibition assay. …”
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Transcriptomic profiling of cytomegalovirus infection in cardiac transplantation: proof-of-concept for a new strategy in tissue markers application by Ilaria Barison, Diego Perazzolo, Chiara Castellani, Alessia Giarraputo, Elisabetta Rossi, Elisabetta Rossi, Luca Vedovelli, Sonia Anna Minuzzo, Chiara Tessari, Nicola Pradegan, Giuseppe Toscano, Francesco Tona, Cristina Basso, Gino Gerosa, Susanna Mandruzzato, Susanna Mandruzzato, Davide Abate, Dario Gregori, Annalisa Angelini, Marny Fedrigo

“…Based on the bioinformatic analysis, we explored the regulatory effects of these miRNAs on the mRNA pathways independently identified in the same samples. …”
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FGFR3-TACC3 fusion gene promotes glioblastoma malignant progression through the activation of STAT3 signaling pathway by Yiming Li, Yiming Li, Jianshen Liang, Jianshen Liang, Jianshen Liang, Xiude Ren, Xiude Ren, Jiahe Guo, Jiahe Guo, Jiahe Guo, Xisen Wang, Xisen Wang, Xisen Wang, Xuya Wang, Xuya Wang, Xuya Wang, Shengping Yu, Shengping Yu, Tao Li, Tao Li, Xuejun Yang, Xuejun Yang, Xuejun Yang

“…A series of phenotypic experiments, including CCK-8, EdU, colony-formation assay, wound healing assay, and transwell assay were conducted to detect the effects of F3-T3 on proliferation, invasion, and migration of glioma cells. …”
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Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling by Nikole J. Byrne, Christoph Koentges, Elisabeth Khan, Katharina Pfeil, Robert Sandulescu, Sayan Bakshi, Carolin Költgen, Ivan Vosko, Johannes Gollmer, Thomas Rathner, Günter Roth, Michael M. Hoffmann, Katja E. Odening, Hauke Horstmann, Luke A. Potter, Christoph Bode, Dennis Wolf, Harald Sourij, Senka Ljubojevic-Holzer, Markus Wallner, Peter P. Rainer, Simon Sedej, Daniel Scherr, Dirk von Lewinski, Adam R. Wende, Andreas Zirlik, Heiko Bugger

“…To current study was designed to explore mechanisms underlying adverse effects of increased SIRT4 levels in the heart following pressure overload. …”
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