Anti‐seasonal flooding drives substantial alterations in riparian plant diversity and niche characteristics in a unique hydro‐fluctuation zone by Ye Liu, Xiaodie Duan, Xiaoling Li, Wenxiong Yi, Gong Chen, Jin Yang, Danli Deng, Xiaojuan Guo, Zhengjian Yang, Guiyun Huang, Meixiang Hu, Chen Ye

“…The results showed anti‐seasonal flooding precipitated the gradual disappearance of the original diverse niches, resulting in the reduction of plant species richness and functional diversity and more obvious competition among plant species with similar resource requirements. …”
Get full text

Comprehensive N-glycosylation profiling of recombinant spike S1 protein from the wild-type SARS-CoV-2 and its variants by Yao Peng, Yao Peng, Yao Peng, Tian-Tian Tong, Qiu-Yu Deng, Lee-Fong Yau, Jia-Qi Qiu, Qing Zhao, Jia-Qi Wu, Zhi-Qiang Xin, Man-Ci Guan, Yue Li, Zhi-Hong Jiang, Hu-Dan Pan, Hu-Dan Pan, Liang Liu, Liang Liu, Liang Liu, Jing-Rong Wang, Jing-Rong Wang

“…UHPLC-TOF MS analysis revealed unoccupied N-glycosylation sites in S1-Gamma (N657), S1-Delta (N61), and S1-Lambda (N17, N61, N657), with N17 and N61 showing low glycan occupancy (0%-3.4%), suggesting these sites may lack glycan shield protection.DiscussionThis study provides a comprehensive N-glycosylation profile of the S1 protein across different variants, offering an essential structural basis for future vaccine development and research on viral functions.…”
Get full text

Experiences of discrimination are associated with microbiome and transcriptome alterations in the gut by Tien S. Dong, Tien S. Dong, Tien S. Dong, Tien S. Dong, Tien S. Dong, Simer Shera, Kirstin Peters, Gilbert C. Gee, Gilbert C. Gee, Hiram Beltrán-Sánchez, Hiram Beltrán-Sánchez, May C. Wang, Lisa A. Kilpatrick, Lisa A. Kilpatrick, Lisa A. Kilpatrick, Lisa A. Kilpatrick, Xiaobei Zhang, Xiaobei Zhang, Xiaobei Zhang, Xiaobei Zhang, Xiaobei Zhang, Jennifer S. Labus, Jennifer S. Labus, Jennifer S. Labus, Jennifer S. Labus, Jennifer S. Labus, Allison Vaughan, Allison Vaughan, Allison Vaughan, Allison Vaughan, Arpana Church, Arpana Church, Arpana Church, Arpana Church, Arpana Church

“…Our random forest classifier demonstrated the capability to accurately differentiate individuals with high and low discrimination in our training cohort (AUC = 0.91).ConclusionThese findings illuminate the substantial impact of discrimination on gut health, encompassing microbiome composition, gene expression, and functional pathways. These findings suggest that discrimination is associated with internal biological changes that can be associated with negative health outcomes, opening research to examine novel pathways that can be used to mitigate the negative health effects of discrimination.…”
Get full text

Targeting IL-33 reprograms the tumor microenvironment and potentiates antitumor response to anti-PD-L1 immunotherapy by Yu Bai, Tao Wu, Jun Lin, Shuwen Xu, Xian Zeng, Xuyao Zhang, Jiajun Fan, Dianwen Ju, Mengyang Li, Yanyang Nan, Xiaozhi Hu, Kaicheng Zhou, An Zhu, Zihan Dou, Zhonglian Cao, Xumeng Zhang, Yuanzhen Zhang, Xuebin Wang

“…Background The main challenge against patients with cancer to derive benefits from immune checkpoint inhibitors targeting PD-1/PD-L1 appears to be the immunosuppressive tumor microenvironment (TME), in which IL-33/ST2 signal fulfills critical functions. However, whether IL-33 limits the therapeutic efficacy of anti-PD-L1 remains uncertain.Methods Molecular mechanisms of IL-33/ST2 signal on anti-PD-L1 treatment lewis lung carcinoma tumor model were assessed by RNA-seq, ELISA, WB and immunofluorescence (IF). …”
Get full text

Sucrose synthase gene family in common bean during pod filling subjected to moisture restriction by Norma Cecilia Morales-Elias, Eleazar Martínez-Barajas, Lilia Angélica Bernal-Gracida, Monserrat Vázquez-Sánchez, Iris Grisel Galván-Escobedo, Jose Salud Rodriguez-Zavala, Amelia López-Herrera, Cecilia Beatriz Peña-Valdivia, Antonio García-Esteva, Carlos Alberto Cruz-Cruz, María Teresa González-Arnao, José Cruz Jiménez-Galindo, Daniel Padilla-Chacón

“…The results provide a systematic overview of the PvSUS gene family in P. vulgaris, offering a framework for further research and the potential functional application of PvSUS genes.…”
Get full text

Myristic acid beneficially modulates intervertebral disc degeneration by preventing endplate osteochondral remodeling and vertebral osteoporosis in naturally aged mice by Yan Gong, Yuzhuo Zhang, Xingda Chen, Zelin Zhou, Weicheng Qin, Yanchi Gan, Jiahui He, Jizhi Ma, Guifeng Chen, Qi Shang, Kai Tang, Honglin Chen, Yu Liu, De Liang, De Liang, Gengyang Shen, Xiaobing Jiang, Zhaojun Cheng, Zhaojun Cheng, Zhaojun Cheng

“…However, the precise mechanism by which MA functions in relation to IDD remains unclear.MethodsIn vivo, a naturally aged animal model was used. …”
Get full text

Circulating innate lymphoid cells are dysregulated in patients with prostate cancer by Daniela Claudia Maresca, Evelina La Civita, Benedetta Romano, Maria Rosaria Ambrosio, Fabio Somma, Tania Wyss, Bernardo Rocco, Valentina Rubino, Luigi Cari, Philippe Krebs, Antonio Rodriguez-Calero, Matteo Ferro, Sara Trabanelli, Camilla Jandus, Felice Crocetto, Angela Ianaro, Daniela Terracciano, Giuseppe Ercolano

“…This study investigated the phenotypic and functional profiles of ILC subsets in the peripheral blood mononuclear cells (PBMCs) of patients with PCa stratified by Gleason score. …”
Get full text

Nationwide implementation of the international multidisciplinary best-practice for locally advanced pancreatic cancer (PREOPANC-4): study protocol by T. F. Stoop, L. W. F. Seelen, F. R. van ’t Land, A. C. van der Hout, J. C. M. Scheepens, M. Ali, A. M. Stiggelbout, B. M. van der Kolk, B. A. Bonsing, D. J. Lips, D. J. A. de Groot, E. van Veldhuisen, E. D. Kerver, E. R. Manusama, F. Daams, G. Kazemier, G. A. Cirkel, G. van Tienhoven, G. A. Patijn, H. N. Lelieveld-Rier, I. H. de Hingh, I. E. G. van Hellemond, J. H. Wijsman, J. I. Erdmann, J. S. D. Mieog, J. de Vos-Geelen, J. W. B. de Groot, K. R. D. Lutchman, L. J. Mekenkamp, L. W. Kranenburg, L. P. M. Beuk, M. W. Nijkamp, M. den Dulk, M. B. Polée, M. Y. V. Homs, M. L. Wumkes, M. W. J. Stommel, O. R. Busch, R. F. de Wilde, R. T. Theijse, S. A. C. Luelmo, S. Festen, T. L. Bollen, U. P. Neumann, V. E. de Meijer, W. A. Draaisma, B. Groot Koerkamp, I. Q. Molenaar, C. L. Wolfgang, M. Del Chiaro, M. G. H. Katz, T. Hackert, J. A. C. Rietjens, J. W. Wilmink, H. C. van Santvoort, C. H. J. van Eijck, M. G. Besselink, for the Dutch Pancreatic Cancer Group

“…Secondary outcomes include quality of life, functioning, side effects, and patients’ healthcare satisfaction in all included patients. …”
Get full text

A Quaternary Sedimentary Ancient DNA (<i>sed</i>aDNA) Record of Fungal–Terrestrial Ecosystem Dynamics in a Tropical Biodiversity Hotspot (Lake Towuti, Sulawesi, Indonesia) by Md Akhtar-E Ekram, Cornelia Wuchter, Satria Bijaksana, Kliti Grice, James Russell, Janelle Stevenson, Hendrik Vogel, Marco J. L. Coolen

“…However, time-series experiments should be supplemented with geobiological archives to capture the long-term effects of environmental changes on fungi–soil–plant interactions, particularly in undersampled, floristically diverse tropical forests. …”
Get full text

PLOD1 promotes the malignancy of hepatocellular carcinoma by facilitating the NF-κB/IL-6/STAT3-dependent TCA cycle by Chengfei Zhang, Yangchun Zhou, Minghua Hu, Yue Pan, Xin Chen, Qi Sun, Zhijie Ma, Cheng Wang, Yong Zha, Feng Zhu, Hongping Xia

“…Herein, we aimed to investigate the underlying biological functions and mechanisms of PLOD1 in HCC. Methods: The expression levels of PLOD1 in HCC were measured by qPCR, Western blot, and immunohistochemistry. …”
Get full text

EFFICIENCY OF DYNAMIC ELECTRONEUROSTIMULATION IN KNEE OSTEOARTHRITIS: RESULTS OF A MULTICENTER RANDOMIZED STUDY by O. M. Lesnyak, E. Yu. Kadochnikova, L. I. Alekseeva, I. G. Dydykina, O. B. Ershova, E. M. Zaitseva, T. A. Korotkova, T. A. Popova, M. L. Sukhareva, E. A. Taskina, V. N. Khodyrev, E. P. Sharapova, A. G. Solodovnikov, A. A. Vlasov

“…Dynamic electroneurostimulation (DENS) is a type of percutaneous electroneurostimulation with a differentiation approach to choosing exposure areas and to optimizing electrocutaneous therapeutic action on the reflexogenic areas and acupuncture points for analgesia and for the treatment of functional disorders.Objective: to study the clinical efficiency and safety of therapy using a DiaDENS-PC apparatus in the treatment of knee osteoarthritis (OA).Subjects and methods. …”
Get full text

Single-cell and spatial atlas of glioblastoma heterogeneity: characterizing the PCLAF+ subtype and YEATS4’s oncogenic role by Siqi Ma, Yuwei Sun, Shaowei Zheng, Yilong Fu, Liangyu Wang, Dun Liu, Henan Jiao, Xuqiang Zhu, Xueyuan Li, Dongming Yan, Di Chen, Zi Ye

“…This subtype promoted therapeutic resistance through unique metabolic reprogramming, MDK-LRP1-mediated microenvironmental interactions, and immunosuppressive properties. YEATS4 knockdown effectively suppressed malignant tumor behaviors, highlighting its therapeutic potential. …”
Get full text

A generative whole-brain segmentation model for positron emission tomography images by Wenbo Li, Zhenxing Huang, Hongyan Tang, Yaping Wu, Yunlong Gao, Jing Qin, Jianmin Yuan, Yang Yang, Yan Zhang, Na Zhang, Hairong Zheng, Dong Liang, Meiyun Wang, Zhanli Hu

“…Moreover, a custom cross-attention module based on a cross-attention mechanism was constructed to effectively fuse the functional information and structural information. …”
Get full text

Cholesteryl Ester Species but Not Serum Proprotein Convertase Subtilisin/Kexin Type 9 Levels Decline in Male Patients with Active Inflammatory Bowel Disease by Angelika Hettenbach, Tanja Elger, Muriel Huss, Gerhard Liebisch, Marcus Höring, Johanna Loibl, Arne Kandulski, Martina Müller, Hauke Christian Tews, Christa Buechler

“…It can be hypothesized that blocking PCSK9 may further reduce serum cholesterol levels, which may have adverse effects in male patients with active IBD.…”
Get full text

<i>Salmonella</i> Typhi-Exposed Placentae: Chorionic Villi Histomorphology and Neonatal Birthweight by Patience B. Tetteh-Quarcoo, Joana Twasam, Kevin Kofi Adutwum-Ofosu, John Ahenkorah, Bismarck Afedo Hottor, Nicholas T. K. D. Dayie, Peter Ofori Appiah, Emmanuel Afutu, Fleischer C. N. Kotey, Emilia Asuquo Udofia, Nii Koney-Kwaku Koney, Benjamin Arko-Boham, Eric S. Donkor

“…Aim: This study utilised stereology to assess histomorphological and functional alterations in term placentae of <i>Salmonella</i> Typhi-exposed placentae, compared to unexposed controls. …”
Get full text

Arabic validation and cross-cultural adaptation of climate anxiety scale by Shymaa Mamdouh Mohamed Abdu, Assem Gebreal, Safar Abadi Alsaleem, Moath S. Aljohani, Suzan Abdel-Rahman, Mohamed Fakhry Hussein, Nada Ibrahim, Iffat Elbarazi, Samah Hussein, Omar Shamma, Ahmed Elsayed said Noureldin, Ramy Mohamed Ghazy

“…Background: Climate change is an enduring global phenomenon that describes a long-lasting effect of change in weather and temperature of the earth. …”
Get full text

Toward generalizable prediction of antibody thermostability using machine learning on sequence and structure features by Ameya Harmalkar, Roshan Rao, Yuxuan Richard Xie, Jonas Honer, Wibke Deisting, Jonas Anlahr, Anja Hoenig, Julia Czwikla, Eva Sienz-Widmann, Doris Rau, Austin J. Rice, Timothy P. Riley, Danqing Li, Hannah B. Catterall, Christine E. Tinberg, Jeffrey J. Gray, Kathy Y. Wei

“…In this work, we show two machine learning approaches – one with pre-trained language models (PTLM) capturing functional effects of sequence variation, and second, a supervised convolutional neural network (CNN) trained with Rosetta energetic features – to better classify thermostable scFv variants from sequence. …”
Get full text

Validation of the PsAID-12 Russian questionnaire in patients with psoriatic arthritis by L. D. Vorobyeva, E. Yu. Loginova, Yu. L. Korsakova, E. E. Gubar, P. O. Tremaskina, T. V. Korotaeva

“…To assess the sensitivity of the questionnaire, its changes were analyzed depending on the achieved effect on therapy after 12 months: group I of patients in whom MDA was achieved – 50 patients; group II – 43 patients REM/LDA; group III – 79 patients with no effect on therapy. …”
Get full text

Delivery of miR-26a-5p by Subcutaneous Adipose Tissue-Derived Extracellular Vesicles Alleviates Acute Lung Injury in Mice Through CHUK/NF-&amp;kappa;B Pathway by Xie Y, Ran L, Yue C, Wang C, Chen F, Su Y, Qin Y, Zhang Q, Liu J, Du N, Zhang L, Jiang Y, Liu G

“…These findings suggest that miR-26a-5p plays a crucial role in the anti-inflammatory effects of SAT-EVs by suppressing CHUK and modulating the NF-κB pathway.Conclusion: SAT-EVs significantly attenuated LPS-induced ARDS, potentially through the CHUK/NF-κB pathway mediated by miR-26a-5p, thereby exerting protective effects against inflammatory lung injury. …”
Get full text

Magnetically sensitive nanocomposites for targeted antitumor therapy with application of gemcitabine by N. M. Korniichuk, S. P. Turanska, A. L. Petranovska, M. V. Abramov, P. P. Gorbyk, N. Yu. Luk'yanova, N. V. Kusyak, V. F. Chekhun

“…To study the direct cytotoxic/cytostatic effect of a series of experimental samples of MF based on PS, Fe3O4 NP, GEM, HER2 AB, as well as MF components in mono- or complex use, onto MDA-MB-231 cells in vitro, IC50 index was determined. …”
Get full text