An Inducible Expression System of the Calcium-Activated Potassium Channel 4 to Study the Differential Impact on Embryonic Stem Cells by Stefan Liebau, Michael Tischendorf, Daniel Ansorge, Leonhard Linta, Marianne Stockmann, Clair Weidgang, Michelina Iacovino, Tobias Boeckers, Götz von Wichert, Michael Kyba, Alexander Kleger

“…The family of calcium-activated potassium channels consists of four members with varying biological functions and conductances. Besides membrane potential modulation, SK channels have been found to be involved in cardiac pacemaker cell development from ES cells and morphological shaping of neural stem cells. …”
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Female-germline specific protein Sakura interacts with Otu and is crucial for germline stem cell renewal and differentiation and oogenesis by Azali Azlan, Li Zhu, Ryuya Fukunaga

“…During oogenesis, self-renewal and differentiation of germline stem cells (GSCs) must be tightly regulated. …”
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FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell by Niu Wang, Weidong Zhang, Zhenyu Zhong, Xiongbo Zhou, Xinran Shi, Xin Wang

“…Hair follicle stem cell (HFSC), capable of self-renewal and differentiation in hair follicle, represents an emerging stem cell model for regenerative medicine. …”
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Simultaneous depletion of RB, RBL1 and RBL2 affects endoderm differentiation of human embryonic stem cells. by Shota Nakanoh, Juned Kadiwala, Laetitia Pinte, Carola Maria Morell, An-Sofie Lenaerts, Ludovic Vallier

“…In contrast, we observed that subsequent differentiation into foregut endoderm was impaired without the expression of RB, RBL1 and RBL2. …”
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A Two-Stage Process for Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells into Neuronal-like Cells by Zaffar Equbal, Prakash N. Baligar, Madhulika Srivastava, Asok Mukhopadhyay

“…Here, we have described a two-stage culture process to differentiate Wharton Jelly mesenchymal stem cells (WJ-MSCs) into neuronal-like cells in the presence of various cues involved in neurogenesis. …”
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Analysis of transcriptome and chromatin accessibility changes during the differentiation of human embryonic stem cells into neural progenitor cells by LI Linying, CAI Xiaodong, TONG Ran, YANG Chen, WANG Zhiming, HE Xiaoyu, MA Ziyue, ZHANG Feng, LI Lingjie, ZHOU Junmei

“…Objective·To investigate the changes in transcriptome and chromatin accessibility during the differentiation of human embryonic stem cells (hESCs) into neural progenitor cells (NPCs) using in vitro differentiation models and high-throughput multi-omics sequencing technologies.Methods·hESCs were first induced to differentiate into NPCs in vitro using the embryoid body formation method, and cells at both stages were collected. …”
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Exploring NamiRNA networks and time-series gene expression in osteogenic differentiation of adipose-derived stem cells by Xin Jin, Yi Lu, Zhihong Fan

“…Background Adipose-derived stem cells (ADSCs) are a type of stem cell found in adipose tissue with the capacity to differentiate into multiple lineages, including osteoblasts. …”
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Inhibition of Protein Farnesylation Arrests Adipogenesis and Affects PPARγ Expression and Activation in Differentiating Mesenchymal Stem Cells by Daniel Rivas, Rahima Akter, Gustavo Duque

“…Protein farnesylation is required for the activation of multiple proteins involved in cell differentiation and function. In white adipose tissue protein, farnesylation has shown to be essential for the successful differentiation of preadipocytes into adipocytes. …”
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The Optimization of a Protocol for the Directed Differentiation of Induced Pluripotent Stem Cells into Liver Progenitor Cells and the Delivery of Transgenes by Irina Panchuk, Valeriia Kovalskaia, Natalia Balinova, Oxana Ryzhkova, Svetlana Smirnikhina

“…The aim of this study was to optimize a protocol for the directed differentiation of induced pluripotent stem cells into liver progenitor cells, achieving the high-level expression of specific markers. …”
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EVL Promotes Osteo-/Odontogenic Differentiation of Dental Pulp Stem Cells via Activating JNK Signaling Pathway by Kangrui Zeng, Qiongyi Kang, Yutong Li, Weiping Li, Qing Cheng, Wenwei Xia

“…The function of EVL in osteo-/odontogenic differentiation and involvement of MAPK signaling pathways were evaluated by alkaline phosphatase (ALP) staining and activity, alizarin red staining (ARS), and qRT-PCR and western blot analyses. …”
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Deciphering the Transcriptional Metabolic Profile of Adipose-Derived Stem Cells During Osteogenic Differentiation and Epigenetic Drug Treatment by Giulia Gerini, Alice Traversa, Fabrizio Cece, Matteo Cassandri, Paola Pontecorvi, Simona Camero, Giulia Nannini, Enrico Romano, Francesco Marampon, Mary Anna Venneri, Simona Ceccarelli, Antonio Angeloni, Amedeo Amedei, Cinzia Marchese, Francesca Megiorni

“…Adipose-derived mesenchymal stem cells (ASCs) are commonly employed in clinical treatment for various diseases due to their ability to differentiate into multi-lineage and anti-inflammatory/immunomodulatory properties. …”
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Differentiation of Stem Cells from Human Exfoliated Deciduous Teeth into Retinal Photoreceptor-Like Cells and Their Sustainability In Vivo by Xiaoxia Li, Jing Xie, Yue Zhai, Tengjiaozi Fang, Nanquan Rao, Shuang Hu, Liping Yang, Yuming Zhao, Yixiang Wang, Lihong Ge

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Gain of 20q11.21 in human pluripotent stem cells enhances differentiation to retinal pigment epithelium by Loriana Vitillo, Fabiha Anjum, Zoe Hewitt, Owen Laing, Nidaa A. Ababneh, Duncan Baker, Ivana Barbaric, Peter J. Coffey

“…We then phenotyped the purified RPE cells for functionality, purity and tumorigenicity potential. Results We observed that 20q11.21 clones exhibited an enhanced differentiation capacity, developing pigmented foci at a higher rate and yield compared to normal clones. …”
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LINC00673 binds with leptin to regulate osteogenic function in periodontal ligament stem cells by Yang Yang, Peng Yang, Yuxing Zhang, Zhaoyan Wu, Xuxia Wang, Jun Zhang

“…Results As predicted, miR-188-3p interacts with LINC00673 directly, and miR-188-3p overexpression increases osteogenic differentiation. However, overexpression of LINC00673 reversed this effect, suggesting that LINC00673 functions as a competing endogenous RNA for miR-188-3p. …”
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Modulation of intracellular kinase signaling to improve TIL stemness and function for adoptive cell therapy by Hao Feng, Ling Qiu, Zixiao Shi, Yao Sheng, Peipei Zhao, Di Zhou, Fei Li, Hailin Yu, Yanan You, Hui Wang, Ming Li, Shurong Zhu, Yan Du, Jun Cui, Jingwei Sun, Yarong Liu, Hua Jiang, Xin Wu

“…This result was further validated by RNA‐seq analysis indicating that AKT1/2 ablation primarily regulates T cell differentiation and function‐related programs. Conclusion Modulation of PI3K‐AKT signaling represents a promising strategy to enhance TIL stemness and cytotoxicity and improve the clinical outcome of current TIL‐based therapy to treat solid tumors.…”
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Transcriptional and Genomic Targets of Neural Stem Cells for Functional Recovery after Hemorrhagic Stroke by Le Zhang, Wenjing Tao, Hua Feng, Yujie Chen

“…However, due to the complexity of NSCs and their microenvironment, current strategies cannot satisfactorily enhance functional recovery after hemorrhagic stroke. It is well known that transcriptional and genomic pathways play important roles in ensuring the normal functions of NSCs, including proliferation, migration, differentiation, and neural reconnection. …”
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Multi-function of adipose-derived stem cells on gut disorder: from bench to bedside by Jiatong Zhao, Shuzi Xin, Xiaohui Liu, Weikai Shi, Boya Wang, Hongli Wang, Yi Wang, Rongxuan Hua, Yize Li, Lei Gao, Xin Lu, Jingdong Xu

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Isolation, Culture, and Functional Characterization of Human Embryonic Stem Cells: Current Trends and Challenges by Firdos Alam Khan, Dana Almohazey, Munthar Alomari, Sarah Ameen Almofty

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Heart Failure Impairs Bone Marrow Hematopoietic Stem Cell Function and Responses to Injury by Tina B. Marvasti, Faisal J. Alibhai, Grace J. Yang, Shu‐Hong Li, Jun Wu, Terrence Yau, Ren‐Ke Li

“…Background Heart failure (HF) is a clinical syndrome associated with a progressive decline in myocardial function and low‐grade systemic inflammation. Chronic inflammation can have lasting effects on the bone marrow (BM) stem cell pool by impacting cell renewal and lineage differentiation. …”
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Rapid neurogenesis through transcriptional activation in human stem cells by Volker Busskamp, Nathan E Lewis, Patrick Guye, Alex HM Ng, Seth L Shipman, Susan M Byrne, Neville E Sanjana, Jernej Murn, Yinqing Li, Shangzhong Li, Michael Stadler, Ron Weiss, George M Church

“…The high purity enabled mRNA and microRNA expression profiling during neurogenesis, thus revealing the genetic programs involved in the rapid transition from stem cell to neuron. The resulting cells exhibited transcriptional, morphological and functional signatures of differentiated neurons, with greatest transcriptional similarity to prenatal human brain samples. …”
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