Cellular fate of a plant virus immunotherapy candidate by Anthony O. Omole, Jessica Fernanda Affonso de Oliveira, Lucas Sutorus, Sweta Karan, Zhongchao Zhao, Barry W. Neun, Edward Cedrone, Jeffrey D. Clogston, Jie Xu, Michael Sierk, Qingrong Chen, Daoud Meerzaman, Marina A. Dobrovolskaia, Nicole F. Steinmetz

“…Abstract Cowpea mosaic virus (CPMV) is a plant virus that is currently being developed for intratumoral immunotherapy. CPMV relieves the immune system from tumor-induced immunosuppression; reprograms the tumor microenvironment to an activated state whereby the treated and distant tumors are recognized and eradicated. …”
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Injectable extracellular vesicle hydrogels with tunable viscoelasticity for depot vaccine by Rimsha Bhatta, Joonsu Han, Yusheng Liu, Yang Bo, Yueji Wang, Daniel Nguyen, Qian Chen, Hua Wang

“…The tumor EV hydrogel also shows synergy with anti-PD-1 checkpoint blockade for tumor treatment, and is able to reprogram the tumor microenvironment. As a proof-of-concept, we also demonstrate that EV hydrogels can induce enhanced antibody responses than solution-form EVs over an extended time. …”
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EPIGENETIC REGULATION BY CURCUMIN IN OVARIAN CANCER: A FOCUS ON miRNA NETWORKS, HISTONE MODIFICATIONS AND DNA METHYLATION by Özlem Timirci Kahraman, Deryanaz Billur, Esin Bayralı Ülker

“…Curcumin selectively reprograms miRNA networks, restoring tumour-suppressive miRNAs while downregulating oncogenic miRNAs, thereby mitigating epithelial-mesenchymal transition and chemoresistance. …”
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Protein Engineering Strategies to Expand CRISPR-Cas9 Applications by Lucas F. Ribeiro, Liliane F. C. Ribeiro, Matheus Q. Barreto, Richard J. Ward

“…The development of precise and modulated methods for customized manipulation of DNA is an important objective for the study and engineering of biological processes and is essential for the optimization of gene therapy, metabolic flux, and synthetic gene networks. …”
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Exogenous citric acid-mediated modification of metabolism, transport and signal transduction of non-structural carbohydrates in rice under hexavalent chromium stress by Yi Kang, Yu-Juan Lin, Cheng-Zhi Li, Hao Zhan, Xiao-Zhang Yu

“…Abstract Background Hexavalent chromium [Cr(VI)] is a highly toxic heavy metal that adversely affects plant growth and development. Non-structural carbohydrates (NSCs) serve as dynamic metabolic buffers under environmental stress, balancing growth and detoxification demands. …”
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Mechanical force receptor Piezo1 regulates TH9 cell differentiation by Qiuli Yang, Yejin Cao, Likun Wang, Yingjie Dong, Longhao Zhao, Zi Geng, Yujing Bi, Guangwei Liu

“…Notably, Piezo1 deficiency inhibited TH9 cell differentiation and contributed to tumor development. Mechanistically, Piezo1 deficiency inhibits TH9 cell differentiation mainly through the SIRT3-succinate dehydrogenase A (SDHA)-oxidative phosphorylation (OXPHOS) pathway. …”
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The Destructive Cycle in Bronchopulmonary Dysplasia: The Rationale for Systems Pharmacology Therapeutics by Mia Teng, Tzong-Jin Wu, Kirkwood A. Pritchard, Billy W. Day, Stephen Naylor, Ru-Jeng Teng

“…We highlight how these intersecting pathways offer not just therapeutic targets but also opportunities for interventions that reprogram system-wide responses. Accordingly, we explore the potential of systems pharmacology therapeutics (SPTs) to address the multifactorial nature of BPD. …”
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EM Dipeptide Enhances Milk Protein Secretion: Evidence from Integrated Metabolomic and Transcriptomic Analysis by Yuqing Liu, Yuhao Yan, Runjun Yang, Xiaohui Li, Chuang Zhai, Xuan Wu, Xibi Fang, Boqun Liu

“…<b>Background/Objectives:</b> Breast milk provides essential nutrition and immune protection to support infant growth and development. However, insufficient breast milk remains a serious issue, and bioactive peptides represent a potential strategy to promote lactation. …”
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Modeling Aberrant Angiogenesis in Arteriovenous Malformations Using Endothelial Cells and Organoids for Pharmacological Treatment by Eun Jung Oh, Hyun Mi Kim, Suin Kwak, Ho Yun Chung

“…Following analysis of these findings, skin samples from 10 AVM patients were reprogrammed into induced pluripotent stem cells (iPSCs) to generate AVM blood vessel organoids. …”
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Closing the loop: establishing an autonomous test-learn cycle to optimize induction of bacterial systems using a robotic platform by Jan Benedict Spannenkrebs, Aron Eiermann, Thomas Zoll, Silke Hackenschmidt, Johannes Kabisch, Johannes Kabisch, Johannes Kabisch

“…To move towards fully automated DBTL cycles, we developed a software framework to enable a robotic platform to autonomously adjust test parameters. …”
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Transcriptional profiling of pediatric cholestatic livers identifies three distinct macrophage populations. by Sarah A Taylor, Shang-Yang Chen, Gaurav Gadhvi, Liang Feng, Kyle D Gromer, Hiam Abdala-Valencia, Kiwon Nam, Salina T Dominguez, Anna B Montgomery, Paul A Reyfman, Lorena Ostilla, Joshua B Wechsler, Carla M Cuda, Richard M Green, Harris Perlman, Deborah R Winter

“…Taken together, these findings may allow for future development of targeted therapeutic strategies to reprogram macrophages to an immune regulatory phenotype and reduce cholestatic liver injury.…”
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Cyclooxygenase-2/prostaglandin E2 inhibition remodulated photodynamic therapy-associated immunosuppression for enhanced cancer immunotherapy by Tao Xu, Kehan Liu, Shuqi Mi, Yao Yao, Mengyao Zhang, Shujuan Xue, Feng Zhi, Sally-Ann Cryan, Dawei Ding

“…To this end, biocompatible albumin nanoassemblies co-delivering IR780 and diclofenac are herein developed for enhanced therapy against triple-negative breast cancer. …”
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L-serine promotes pro-carcinogenic effects of colibactin-producing E. coli by Amandine Devaux, Romain Villéger, Gwenaëlle Roche, Binta Diémé, Michael Rodrigues, Charline Juban, Marie Lagrée, Nicolas Venisse, Cyril Jousse, Nicolas Barnich, Mathilde Bonnet

“…Here, we report that CoPEC reprograms the metabolism of colonic epithelial cells in a colibactin-dependent manner, leading to a Warburg-like effect, altered redox homeostasis, and disrupted amino acid metabolism. …”
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Effective therapeutic targeting of tumor lineage plasticity in neuroendocrine prostate cancer by BRD4 inhibitors by Xiong Zhang, Yatian Yang, Hongye Zou, Yang Yang, Xingling Zheng, Eva Corey, Amina Zoubeidi, Nicolas Mitsiades, Ai-Ming Yu, Yuanpei Li, Hong-Wu Chen

“…Interestingly, BRD4 genome occupancy is reprogrammed by anti-AR drugs from facilitating AR function in CRPC cells to activating the LP programs and is facilitated by pioneer factor FOXA1. …”
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The Critical Role of Adipocytes in Leukemia by Romane Higos, Kevin Saitoski, Mathieu Hautefeuille, Geneviève Marcelin, Karine Clément, Nadine Varin-Blank, Christophe Breton, Simon Lecoutre, Mélanie Lambert

“…The bone marrow microenvironment is a dynamic and complex niche that plays a central role in the development, progression, and therapeutic resistance of leukemia. …”
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Single-cell Analysis Highlights Anti-apoptotic Subpopulation Promoting Malignant Progression and Predicting Prognosis in Bladder Cancer by Linhuan Chen, Yangyang Hao, Tianzhang Zhai, Fan Yang, Shuqiu Chen, Xue Lin, Jian Li

“…A 5-gene risk model was developed to predict patient prognosis, which was significantly associated with immune checkpoint gene expression, suggesting potential implications for immunotherapy. …”
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BET Protein Inhibition Relieves MDSC-Mediated Immune Suppression in Chronic Lymphocytic Leukemia by Erin M. Drengler, Audrey L. Smith, Sydney A. Skupa, Elizabeth Schmitz, Eslam Mohamed, Dalia El-Gamal

“…MDSCs are immature myeloid cells that are hijacked during development and further reprogrammed by the tumor microenvironment (TME) to harbor immune-suppressive properties and inhibit T-cell functions. …”
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The distinct landscape of tumor immune microenvironment in homologous recombination deficient cancers by Qiuyang Xu, Yuanjia Wen, Taiyuan Huang, Huayi Li, Xingzhe Liu, Shen-nan Shi, Wenjian Gong, Gordon B. Mills, Ding Ma, Qinglei Gao, Yong Fang

“…This intricate network reprograms cancer biology in multiple aspects and serves as a potential target for cancer treatment. …”
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ACSL1 Aggravates Thromboinflammation by LPC/LPA Metabolic Axis in Hyperlipidemia Associated Myocardial Ischemia‐Reperfusion Injury by Shuai Jiang, Xueguang Lin, Bo Chen, Gang Chen, Kristine J.S. Kwan, Jing Liu, Qi Sun, Jie Wang, Yijie Lu, Jindong Tong, Ying Deng, Bo Yu, Jingdong Tang

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Nanoparticle delivery of VEGF-B mRNA promotes T cell infiltration within tumor and triggers robust antitumor immunity by Geqiang Zhang, Jun Tu, Yu Zhang, Jianli He, Guoyuan Peng, Qiuju Fan, Yirong Zhang, Mingming Zhang, Hongsheng Tan, Yingjie Xu, Jinke Cheng

“…The advancement of mRNA-based cancer immunotherapies has gained significant momentum, particularly after the success of mRNA vaccines during the COVID-19 pandemic and the recognition of mRNA vaccine development with the 2023 Nobel Prize. mRNA encoding cytokines, antibodies, and chimeric antigen receptor T cells has demonstrated substantial therapeutic potential in both preclinical models and clinical trials. …”
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