Expression and Prognostic Significance of PDIA3 in Cervical Cancer by Jing Zhang, Hui Li, Huling Li, Dandan Lin, Xiaoyan Wang, Kai Wang

“…Immunohistochemistry was used to detect the PDIA3 expression in cervical cancer tissue microarray, including 111 cancer tissue samples and 24 adjacent cancer tissue samples, and the relationship between PDIA3 protein expression and clinical characteristics of patients with cervical cancer was analyzed. …”
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Disulfidptosis-related gene in acute myocardial infarction and its diagnostic value and functions based on bioinformatics analysis and machine learning by Liwen Chen, Jinru Wei, Jinru Wei, Guoxiong Deng, Guoxiong Deng, Guien Xu

“…This study aims to identify disulfidptosis-related genes in AMI and assess their diagnostic potential using bioinformatics and machine learning.MethodsThe microarray datasets GSE60993 and GSE61144, associated with Acute Myocardial Infarction (AMI), were obtained from the Gene Expression Omnibus (GEO) database. …”
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Modulation of homologous recombination gene activity in breast tumor cells in an <i>in vitro</i> model by M. M. Tsyganov, A. A. Frolova, E. A. Kravtsova, I. A. Tsydenova, M. K. Ibragimova

“…To assess the presence of chromosomal aberrations, microarray analysis was performed on DNA chips.Results. …”
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Long read whole genome sequencing-based discovery of structural variants and their role in aetiology of non-syndromic autism spectrum disorder in India by Jhanvi Shah, Debasrija Mondal, Deepika Jain, Priti Mhatre, Ketan Patel, Anand Iyer, Manoj Pandya, Bhargavi Menghani, Gayatri Dave, Jayesh Sheth, Frenny Sheth, Shweta Ramdas, Harsh Sheth

“…Short read sequencing (SRS) and chromosomal microarray (CMA) are unable to resolve these SVs due to their inherent technological limitations. …”
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A first large study of whole-exome sequencing (WES) in 489 patients with suspected rare genetic disorders at a tertiary centre in Malaysia by Lip Hen Moey, Go Hun Seo, Boon Eu Cheah, Wee Teik Keng, Hane Lee, Gaik Siew Ch’ng

“…Whole exome sequencing (WES) is a comprehensive test that can detect not only many of the same large deletions and duplications as CMA, but also detect other genetic variants that are detected by gene panels. …”
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Resistome and Phylogenomics of <i>Escherichia coli</i> Strains Obtained from Diverse Sources in Jimma, Ethiopia by Mulatu Gashaw, Esayas Kebede Gudina, Guenter Froeschl, Ralph Matar, Solomon Ali, Liegl Gabriele, Amelie Hohensee, Thomas Seeholzer, Arne Kroidl, Andreas Wieser

“…Isolates exhibiting phenotypic resistance to beta-lactam antibiotics were further analyzed with a DNA microarray to confirm the presence of resistance-encoding genes. …”
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Circular RNA APP contributes to Alzheimer’s disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis by Deng-Pan Wu, Yan-Su Wei, Li-Xiang Hou, Yu-Xuan Du, Qiu-Qing Yan, Ling-Ling Liu, Yuan-Dan Zhao, Ru-Yu Yan, Chao Yu, Zhen-Guo Zhong, Jin-Lan Huang

“…RT-PCR assay was conducted to detect the expression of circAPP, miR-1906 and CLIC1. …”
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CD226+ B cells in primary Sjögren’s syndrome: a key player in clinical manifestations and disease pathogenesis by Ping Zhao, Ping Zhao, Saizhe Song, Song Zhang, Cheng Peng, Wei Cheng, Xin Chang, Xin Chang, Changhao Xie, Zhongli Hu, Cuiping Liu

“…Multicolor immunofluorescence staining was applied to detect the co-expression of B cells and CD226 in the salivary gland of pSS patients.Microarray analysis was conducted to analyze the transcriptomic profiles of sorted CD226+ CD19+ B cells and CD226- CD19+ B cells.ResultsCD226 expression in the peripheral blood of pSS patients was significantly increased on T cells, CD19+ B cells and CD14+ monocytes, but significantly decreased on CD56+ NK cells.We identified a distinct CD226+CD19+ B cell subset that exhibited pathogenic features in pSS. …”
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Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway by Yang Sheng Wu, Zhe Ming Li, Yi Tao Chen, Shi Jie Dai, Xiao Jie Zhou, Yuan Xiao Yang, Jian Shu Lou, Li Ting Ji, Yu Ting Bao, Ling Xuan, Lu Ning Lin, Chang Yu Li

“…Microarray analysis of liver tissues was performed, and differentially expressed gene (DEG) levels were examined by quantitative real-time PCR (qPCR) and Western blot. …”
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Disruption of bioenergetics enhances the radio-sensitivity of patient-derived glioblastoma tumorspheres by Seo Jin Kim, Junseong Park, Jin-kyoung Shim, Ran Joo Choi, Ju Hyung Moon, Eui Hyun Kim, Wan-Yee Teo, Jong Hee Chang, Seok-Gu Kang

“…Methods: Gene expression profiles of GBM patient-derived TSs were obtained through microarray and RNA-seq. In vitro treatment efficacy was assessed using clonogenic assay, 3D invasion assay, neurosphere formation assay, and flow cytometry. …”
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Autoantibodies Targeting Vinculin Reveal Novel Insight into the Mechanisms of Autoimmune Podocytopathies by Hanyan Meng, Dongjie Wang, Chen Zheng, Chao Zhou, Xinrui Mao, Jinglan Gu, Xiaohui Qiao, Fei Liu, Jingjing Wang, Haidong Fu, Jianhua Mao, Qing Ye

“…Methods: Serum anti-vinculin autoantibody levels detected by protein microarray and clinical data were compared among INS patients (n = 147), healthy individuals (n = 84), and patients with other kidney or immune diseases (n = 100 of each disease). …”
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Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy by Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du

“…Methods The expression of CD155 was analyzed across various cancer types using data from The Cancer Genome Atlas (TCGA) and validated by tissue microarray analysis. The impact of CD155 on T cell mediated cytotoxicity was analyzed using CD155 over-expression or knockout tumor cells. …”
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Study on the mechanisms and functions of lncRNAs in regulating carboplatin resistance in ovarian cancer cells by HONG Yi, CHEN Cuilan, YIN Fuqiang, LIU Xia

“…Methods Differential expressed lncRNAs were screened using expression profiling microarrays in two pairs of carboplatin-resistant and sensitive OC cells (HeyA8-CBP/HeyA8 and SKOV3-CBP/SKOV3). …”
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Long Noncoding RNA Expression Profiles of Periodontal Ligament Stem Cells from the Periodontitis Microenvironment in Response to Static Mechanical Strain by Jia Liu, Yan Zhao, Qiannan Niu, Ni Qiu, Shuangyun Liu, Chunrong Li, Cuixia Li, Pei Miao, Libo Yan, Qiang Li, Zuolin Jin

“…First, we subjected PDLSCs obtained from healthy periodontal tissues (HPDLSCs) and PPDLSCs to static mechanical strain (SMS) with 12% elongation at 0.1 Hz frequency using an FX-4000T system and screened overall lncRNA profiles in both cell types by microarray. Among lncRNAs with a fold change FC>20.0, 27 lncRNAs were upregulated in strained HPDLSCs, and 16 lncRNAs (9 upregulated and 7 downregulated) were detected in strained PPDLSCs. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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In vivo RNAi screen and validation reveals Ngp, Hba-a1, and S100a8 as novel inhibitory targets on T lymphocytes in liver cancer by Inga Hochnadel, Lisa Hoenicke, Nataliia Petriv, Huizhen Suo, Lothar Groebe, Chantal Olijnik, Nina Bondarenko, Nina Bondarenko, Juan C. Alfonso, Michael Jarek, Ruibing Shi, Andreas Jeron, Andreas Jeron, Kai Timrott, Tatjana Hirsch, Nils Jedicke, Dunja Bruder, Dunja Bruder, Frank Klawonn, Frank Klawonn, Frank Klawonn, Ralf Lichtinghagen, Robert Geffers, Henrike Lenzen, Henrike Lenzen, Michael P. Manns, Tetyana Yevsa

“…Therefore, in this study, we aimed to define novel ICI molecules arising on T cells during aggressive HCC development.MethodsUsing autochthonous HCC models, we performed microarray analyses followed by in vivo RNA interference screen and identified several new ICI molecules on CD4 and CD8 T lymphocytes in HCC-bearing mice. …”
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Molecular profiling analysis for colorectal cancer patients with Pi-Xu or Shi-Re syndrome by Xinbing Sui, Yong Guo, Wei Ni, Haiyan Jin, Haoming Lin, Tian Xie

“…The protein expression was determined by Immunohistochemistry (IHC) staining and mRNA profiling was detected by expression microarray. Furthermore, mRNA fold change was evaluated by qRT-PCR. …”
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Etiology and outcomes of fetal renal abnormalities in Southern China: a single-tertiary-center study by Meiying Cai, Yashi Gao, Huili Xue, Xianguo Fu, Hua Cao, Liangpu Xu, Na Lin, Hailong Huang

“…We retrospectively analyzed data from 1,019 cases for which chromosomal microarray analysis (CMA) was performed; 58 CMA-negative fetuses were selected for whole-exome sequencing (WES). …”
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Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5’tRFAla/DVL/β-catenin pathway inhibition by Zhipeng Zhang, Yanmei Wu, Wenqiang Liang, Zhifang Liao, Hongbo Liao, Xingxing Xing, Wenxin Yi, Zixuan Liu, Yicheng Li, Mengya Shi, Dongling Lin, Ting Gu, Biao Wu, Mingzhi Zou, Huilai Miao, Xin Wu

“…Subsequently, two new tsRNAs, namely 5’tRFAla and 5’tiRNAAla, which match well with the mRNAs of two pivotal upstream regulators (DVL2 and DVL3) of β-catenin based on bioinformatics analyses, were detected to be significantly decreased in ELT-treated PLC/PRF/5 cells using Arraystar small RNA microarray analyses. …”
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MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
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