Genomic insights into the population structure, antimicrobial resistance, and virulence of Brachyspira hyodysenteriae from diverse geographical regions by Maria Hakimi, Fangshu Ye, Anugrah Saxena, Xiao Hu, Huigang Shen, Paarthiphan Elankumaran, Chloe C. Stinman, Nubia Macedo, Orhan Sahin, Eric R. Burrough, Ganwu Li

“…The pan-genome exhibited a high proportion of hypothetical genes, necessitating further functional characterization. Antimicrobial resistance (AMR) screening detected the tva(A) and lnu(C) genes associated with tiamulin and lincomycin resistance, respectively, in specific lineages and STs. …”
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Evaluation of Simulated Weightlessness Model of Hindlimb Unloading Miniature Pigs and Their Tissue Damage by TU Yingxin, JI Yilan, WANG Fei, YANG Dongming, WANG Dongdong, SUN Zhixin, DAI Yuexin, WANG Yanji, KAN Guanghan, WU Bin, ZHAO Deming, YANG Lifeng

“…Additionally, western blotting was employed to detect the expression levels of skeletal muscle atrophy-related proteins, including muscle-specific RING finger protein 1 (MuRf-1) and muscle atrophy F-box (MAFbx, as known as Atrogin-1), while immunohistochemistry was used to detect the expression of glial fibrillary acidic protein (GFAP), an indicator of astrocyte activation in the brain, reflecting the pathophysiological functional changes across systems. …”
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Differential involvement of neurotransmitter pathways in AD, bvFTD and MCI: Whole-brain MRI analysis by Ricardo Félix Morais, José Maria Sousa, Cemal Koba, Leon Andres, Tiago Jesus, Inês Baldeiras, Tiago Gil Oliveira, Isabel Santana

“…In MCI, early serotonergic dysregulation involving SERT occurs before detectable atrophy.…”
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Transcriptome Analysis Reveals the Pivotal Genes and Regulation Pathways Under Cold Stress and Identifies <i>SbERF027</i>, an AP2/ERF Gene That Confers Cold Tolerance in Sorghum by Qijin Lou, Peifeng Wang, Miao Yu, Zhigan Xie, Chen Xu, Shengyu Chen, Hao Yu, Rui Zhang, Guangling Tian, Di Hao, Xianshi Ke, Shuai Yu, Jiajia Zhou, Yao Zhao, Chao Ye, Jiyuan Guo, Haiyan Zhang, Mo Chen, Xingbei Liu

“…Additionally, the AP2/ERF family gene <i>SbERF027</i>, a novel regulator of cold tolerance, was functionally identified through a comprehensive analysis. …”
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Integrating parental genomes to reduce reference bias and identify intramuscular fat genes in Qinchuan Black pigs by Guangquan Lv, Peiyu Yang, Ao Guo, Minghao Cao, Dong Li, Zhe Liu, Mingyu Wang, Jingchun Sun, Rongrong Ding, Taiyong Yu

“…The uniformity of variant distribution and genome functional annotation remained consistent before and after integration, while the ratio of non-reference/non-reference genotypes was also improved. …”
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Identification of the Cinnamyl Alcohol Dehydrogenase Gene Family in <i>Brassica</i> U-Triangle Species and Its Potential Roles in Response to Abiotic Stress and Regulation of Seed... by Yiwei Liu, Ziwuyun Weng, Yuanyuan Liu, Mengjiao Tian, Yaping Yang, Nian Pan, Mengzhen Zhang, Huiyan Zhao, Hai Du, Nengwen Yin, Cunmin Qu, Huafang Wan

“…Cinnamyl alcohol dehydrogenase (CAD) is essential for lignin precursor synthesis and responses to various abiotic stresses in plants. However, the functions of CAD in <i>Brassica</i> species, especially in <i>Brassica napus</i>, remain poorly characterized. …”
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Ecotoxicological implications of agricultural ecosystem sedimentary arsenic exposure in benthic organisms: A case study on apple snail by Xiaoyang Bi, Binran Wang, Jingyi Guo, Junhao Qin, Huashou Li, Dongqin Li, Guikui Chen, Rongliang Qiu

“…Arsenic (As), a metalloid widely distributed in the environment, remains underexplored regarding its ecotoxicological effects on benthic organisms, especially those exposed to agricultural ecosystem sedimentary As. …”
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CD226+ B cells in primary Sjögren’s syndrome: a key player in clinical manifestations and disease pathogenesis by Ping Zhao, Ping Zhao, Saizhe Song, Song Zhang, Cheng Peng, Wei Cheng, Xin Chang, Xin Chang, Changhao Xie, Zhongli Hu, Cuiping Liu

“…This study aims to elucidate the functional contribution of CD226+ B cells to pSS development and their clinical relevance.MethodsThe percentages of CD226 on T cells, B cells, CD56+ NK cells and CD14+ monocytes in the peripheral blood(PB) of pSS patients and healthy controls (HCs) were detected by flow cytometry.Multicolor flow cytometry was employed to examine the distribution of CD226 in B cell subsets of pSS patients, as well as the expression levels of co-stimulatory molecules, activation and proliferation markers, immunoglobulins, and pro-inflammatory cytokines on both CD226+ B cells and CD226- B cells. …”
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Differential Expression Characteristics of Two Isoforms <i>nr5a2f</i> and <i>nr5a2m</i> in Gonadal Differentiation of Chinese Giant Salamanders, <i>Andrias davidianus</i> by Dan Hu, Guanglve Li, Guohua Zou, Jiaqing Xu, Wenyin Luo, Qiaomu Hu

“…In this study, the full length of <i>Nr5a2f</i> and <i>Nr5a2m</i> were obtained by <i>Nr5a2</i> variable splicing from <i>Andrias davidianus</i>, and the tissue distribution was detected. We identified <i>Nr5a2f</i> of 2455 bp and <i>Nr5a2m</i> of 2150 bp length, encoding 479 and 325 amino, respectively. …”
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CD81-guided heterologous EVs present heterogeneous interactions with breast cancer cells by Elena Gurrieri, Giulia Carradori, Michela Roccuzzo, Michael Pancher, Daniele Peroni, Romina Belli, Caterina Trevisan, Michela Notarangelo, Wen-Qiu Huang, Agata S. A. Carreira, Alessandro Quattrone, Guido Jenster, Timo L. M. Ten Hagen, Vito Giuseppe D’Agostino

“…Interestingly, the fusion protein-corresponding transcripts present as full-length mRNAs in recombinant EVs could reach orthotopic breast tumors in JIMT-1-xenografted mice, improving our sensitivity in detecting penetrant cargoes in tissue biopsies. Conclusions This study highlights the quantitative aspects underlying the creation of a platform for secreted heterologous EVs and shows the limits of single receptor-ligand interactions behind EV-cell engagement mechanisms, which now become the pivotal step to predict functional tropism and design new generations of EV-based nanovehicles.…”
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Dietary supplementation with L-carnitine elevates intracellular carnitine levels and affects gene expression of SLC25A20 and COX4I1, as well as non-mitochondrial respiration of bov... by Leonie Seemann, Susanne Kersten, Susanne Bühler, Fabian Billenkamp, Ulrich Meyer, Christian Visscher, Korinna Huber, Erika Most, Klaus Eder, Sven Dänicke, Jana Frahm

“…The same applied for PBMC mitochondrial functionality and metabolic activity as assessed by ex vivo approaches, whereas non-mitochondrial respiration rate was significantly affected by L-carnitine supplementation over time. …”
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Oral administration of antimicrobial peptide MPX can effectively regulate LPS-mediated testicular oxidative stress and blood‒testis barrier damage by Chunling Zhu, Chengshui Liao, Yilin Bai, Rui Yang, Boyang Zhang, Xueqin Zhao, Wei Zhang, Xiaojing Xia, Huihui Zhang, Huarun Sun, Weiyu Luo, Jianhe Hu, Yuliang Wen, Yueyu Bai, Lei Wang, Ke Ding, Xueming Zhang

“…Abstract Oxidative stress and disruption of blood‒testis barrier permeability are considered key factors in the pathogenesis of testicular inflammation, degeneration, and functional impairment, which play crucial roles in male infertility. …”
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Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.) by Muslim Qadir, Muslim Qadir, Muslim Qadir, Xinyi Lin, Farhan Nabi, Kishore Kumar Ashok, Xue-Rong Zhou, Qingbin Sun, Peiman Shi, Xinfa Wang, Jiaqin Shi, Hanzhong Wang

“…Linkage mapping identified ten QTLs that were distributed on eight chromosomes and explained 7.0-15.9% of the phenotypic variance. …”
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Drone mapping links reindeer browsing during an herbivory pulse to divergent vegetation community responses by Marcus P Spiegel, Jeffrey T Kerby, Dorothée Ehrich, Alexander Volkovitskiy, Alexandra Terekhina, Violetta Filippova, Kirill Shklyar, Natalia Sokolova, Aleksandr A Sokolov, Marc Macias-Fauria

“…Large herbivores regulate ecosystem structure and functioning across Earth’s biomes, but vegetation community responses to herbivory depend on complex interactions involving the timing and intensity of herbivory pressure and other, often abiotic, controls on vegetation. …”
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Design of a monitoring program to advance nightjar conservation along the Atlantic Flyway by Marie I. Tosa, Anthony J. Roberts, Amy K. Tegeler, Patrick K. Devers, Logan A. M. Parker, Pamela D. Hunt, Bryan D. Watts, Min Huang, Andrew C. Vitz, Sara H. Schweitzer, Samantha Robinson, Matthew D. Palumbo, Ryan Rebozo

“…We found all tested sample sizes (i.e., number of routes) within the Atlantic Flyway were sufficient to correctly classify increasing and declining population trends, but none could detect a slight trend. Nearly 70% of randomly distributed routes within the flyway had existing nightjar survey routes within 20 miles and >90% had existing BBS routes within 20 miles. …”
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Psychometric properties and meaningful change thresholds for the QOL-E instrument in patients with myelodysplastic neoplasms by Esther Natalie Oliva, Shien Guo, Jennifer Lord-Bessen, Aylin Yucel, Roberto Latagliata, Massimo Breccia, Giuseppe A. Palumbo, Grazia Sanpaolo, Marta Riva, Valeria Santini, Uwe Platzbecker, Guillermo Garcia-Manero, Pierre Fenaux, Christopher G. Pelligra

“…It contains 29 items with the first two items assessing the patient’s general well-being and the 27 remaining items grouped into six domain scores: physical well-being (QOL-FIS), functional well-being (QOL-FUN), social/family well-being (QOL-SOC), sexual well-being (QOL-SEX), fatigue (QOL-FAT), and MDS-specific disturbances (QOL-MDSS). …”
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Fluorescence lifetime imaging microscopy approach reveals quantitative signatures for hepatocellular carcinoma diagnosis by Lingyun Li, Lingyun Li, Aoshan Wang, Xiongqing Liu, Ganlu Wang, Shanjie Xu, Changjiang Li, Wenzhong Wu, Xiaoying Zhang, Zhendan He, Huiling Qiu, Xiao Peng, Wei Yan, Junle Qu

“…We employed FLIM to compare the fluorescence lifetime distributions between the cancerous regions and the corresponding peritumoral regions. …”
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EGFR-mediated crosstalk between vascular endothelial cells and hepatocytes promotes Piezo1-dependent liver regeneration by Yuelei Hu, Guifang Du, Chao Li, Rui Wang, Juan Liu, Yunfang Wang, Jiahong Dong

“…Hepatocyte proliferation is essential for recovering liver function after injury. In liver surgery, the mechanical stimulation induced by hemodynamic changes triggers vascular endothelial cells (VECs) to secrete large amounts of cytokines that enhance hepatocyte proliferation and play a pivotal role in liver regeneration (LR). …”
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Molecular Cloning and Expression Characteristics of otx2 and eya1 During the Early Development of Seriola aureovittata by Yan JIANG, Xin LIU, Yongjiang XU, Aijun CUI, Bin WANG, Xinfu LIU, Xuezhou LIU, Zhiyong XUE

“…Yellowtail kingfish (Seriola aureovittata) is a pelagic migratory fish species with a global distribution. In recent years, interest in the aquaculture of this species has increased worldwide because of its high flesh quality and fast growth. …”
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Novel insecticide resistance mutations associated with variable PBO synergy in Anopheles gambiae s.l. from the Democratic Republic of Congo by Bethanie Pelloquin, Fiacre Agossa, Holly Acford-Palmer, Tiffany Clark, Sheila B. Ogoma, Miriam Williams, Narcisse Basosila, Francis Watsenga, Emery Metelo, Richard M. Oxborough, Yibayiri Osee Sanogo, Ferdinand Ntoya, Emma Collins, Sophie Moss, Charles McLoughlin, Monica Campos, Jody Phelan, Mark Rowland, Thomas Walker, Noboru Minakawa, Emile Zola Manzambi, Eric Mukomena, Susana Campino, Louisa A. Messenger

“…Nationwide geographical trends in insecticide resistance mutation distribution, prevalence and selection were evident. …”
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