Distribution of Genetic Determinants Associated with CRISPR-Cas Systems and Resistance to Antibiotics in the Genomes of Archaea and Bacteria by Laura Antequera-Zambrano, Ángel Parra-Sánchez, Lenin González-Paz, Eduardo Fernandez, Gema Martinez-Navarrete

“…Through bioinformatic analyses of CRISPR arrays, <i>cas</i> gene architectures, direct repeats (DRs), and thermodynamic properties, we observed that Archaea exhibit a higher number and greater complexity of CRISPR loci, with more diverse cas gene subtypes exclusively of Class 1. …”
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An immunomics approach to schistosome antigen discovery: antibody signatures of naturally resistant and chronically infected individuals from endemic areas. by Soraya Gaze, Patrick Driguez, Mark S Pearson, Tiago Mendes, Denise L Doolan, Angela Trieu, Donald P McManus, Geoffrey N Gobert, Maria Victoria Periago, Rodrigo Correa Oliveira, Fernanda C Cardoso, Guilherme Oliveira, Guilherme Oliveira, Rie Nakajima, Al Jasinskas, Chris Hung, Li Liang, Jozelyn Pablo, Jeffrey M Bethony, Philip L Felgner, Alex Loukas

“…Using well-established Brazilian cohorts of putatively resistant (PR) and chronically infected (CI) individuals stratified by the intensity of their S. mansoni infection, we probed arrays for IgG subclass and IgE responses to these antigens to detect antibody signatures that were reflective of protective vs. non-protective immune responses. …”
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Suppression of Inflammation by Si Miao San in Experimental Rheumatoid Arthritis Through Modulation of the AKT/ROS/Autophagy Axis by Zhang H, Jiang H, Wei Q, Xu J, Zhao Z, Sun Y, Lu Q

“…The expression of NE, MPO, PAD4, LC3B, CitH3, p-AKT and p-PI3K and the production of ROS were detected using IHC, WB and IF analyses. Cytokines in the sera of the mice were detected using cytometric bead arrays. …”
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Cutibacterium acnes strains associated with bone prosthesis infections cannot evade the host immune system by Léa Thoraval, Min Tang-Fichaux, Christine Guillaume, Jennifer Varin-Simon, Claire Dumortier, Johan Sergheraert, Fabien Lamret, Mélanie Bonhomme, Frédéric Laurent, Jérôme Josse, Sophie C. Gangloff, Céline Mongaret, Fany Reffuveille, Frédéric Velard

“…We investigated the ability of these C. acnes strains to trigger in vitro human primary neutrophils (PMN) response through inflammatory mediators measurements (antibody arrays, ELISA, RT-qPCR, zymography) and activation status assessment (flow cytometry), and to induce in vivo PMN recruitment from the bloodstream in mice air-pouch model. …”
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Exonic and Intronic <i>WNT10A</i> Variants Isolated from Korean Children with Non-Syndromic Tooth Agenesis by Yeonjin Ju, Joo Yeon Lee, Woochang Hwang, Jonghyun Shin, Hyung-Sik Kim, Junho K. Hur, Eungyung Lee

“…<b>Results</b>: Mutations were detected exclusively in the patient samples: 629C>G and 1100C>T in exon 1, 1977T>C in intron 1, 10256C>T and 10382G>A in exon 3, and 15953G>A in intron 4. …”
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Emergence of concurrently transmissible mcr-9 and carbapenemase genes in bloodborne colistin-resistant Enterobacter cloacae complex isolated from ICU patients in Kolkata, India by Gourab Halder, Bhaskar Narayan Chaudhury, Priyanka Denny, Mandira Chakraborty, Subhranshu Mandal, Shanta Dutta

“…Diverse phylogenetic clones along with novel sequence types were detected. This study highlights the necessity for intense monitoring of mcr-9 in conjunction with the existing epidemic clones of CR-ECC strains harboring diverse arrays of transmissible AMR genes.…”
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Silicon On-Chip One-Dimensional Photonic Crystal Nanobeam Bandgap Filter Integrated With Nanobeam Cavity for Accurate Refractive Index Sensing by Daquan Yang, Chuan Wang, Yuefeng Ji

“…Thus, the method presented here is promising to build highly parallel integrated sensor arrays for lab-on-a-chip applications and accurate detections.…”
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Large genomic rearrangements of BRCA1 and BRCA2 among patients referred for genetic analysis in Galicia (NW Spain): delimitation and mechanism of three novel BRCA1 rearrangements. by Laura Fachal, Ana Blanco, Marta Santamariña, Angel Carracedo, Ana Vega

“…All the BRCA1 LGRs arose from unequal homologous recombination events involving Alu elements. We also detected, by sequencing, one BRCA2 LGR, the Portuguese founder mutation c.156_157insAluYa5. …”
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Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew. by Lukas Kunz, Jigisha Jigisha, Fabrizio Menardo, Alexandros G Sotiropoulos, Helen Zbinden, Shenghao Zou, Dingzhong Tang, Ralph Hückelhoven, Beat Keller, Marion C Müller

“…We show that AvrPm60 is encoded by three tandemly arrayed, nearly identical effector genes that trigger an immune response upon co-expression with Pm60 and its alleles Pm60a and Pm60b. …”
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Analysis of Proteins and Piwi-Interacting RNA Cargo of Extracellular Vesicles (EVs) Isolated from Human Nose Organoids and Nasopharyngeal Secretions of Children with RSV Infections by Tiziana Corsello, Nicholas Dillman, Yingxin Zhao, Teodora Ivanciuc, Tianshuang Liu, Antonella Casola, Roberto P. Garofalo

“…A total of 490 piRNAs were detected by NGS sequencing of small RNA libraries obtained from NPS-EVs, which has not been reported prior to this study. …”
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The NANOGrav 15 yr Data Set: Looking for Signs of Discreteness in the Gravitational-wave Background by Gabriella Agazie, Akash Anumarlapudi, Anne M. Archibald, Zaven Arzoumanian, Jeremy George Baier, Paul T. Baker, Bence Bécsy, Laura Blecha, Adam Brazier, Paul R. Brook, Lucas Brown, Sarah Burke-Spolaor, J. Andrew Casey-Clyde, Maria Charisi, Shami Chatterjee, Tyler Cohen, James M. Cordes, Neil J. Cornish, Fronefield Crawford, H. Thankful Cromartie, Kathryn Crowter, Megan E. DeCesar, Paul B. Demorest, Heling Deng, Timothy Dolch, Elizabeth C. Ferrara, William Fiore, Emmanuel Fonseca, Gabriel E. Freedman, Nate Garver-Daniels, Peter A. Gentile, Joseph Glaser, Deborah C. Good, Kayhan Gültekin, Jeffrey S. Hazboun, Ross J. Jennings, Aaron D. Johnson, Megan L. Jones, Andrew R. Kaiser, David L. Kaplan, Luke Zoltan Kelley, Matthew Kerr, Joey S. Key, Nima Laal, Michael T. Lam, William G. Lamb, Bjorn Larsen, T. Joseph W. Lazio, Natalia Lewandowska, Tingting Liu, Duncan R. Lorimer, Jing Luo, Ryan S. Lynch, Chung-Pei Ma, Dustin R. Madison, Alexander McEwen, James W. McKee, Maura A. McLaughlin, Natasha McMann, Bradley W. Meyers, Patrick M. Meyers, Chiara M. F. Mingarelli, Andrea Mitridate, Priyamvada Natarajan, Cherry Ng, David J. Nice, Stella Koch Ocker, Ken D. Olum, Timothy T. Pennucci, Benetge B. P. Perera, Nihan S. Pol, Henri A. Radovan, Scott M. Ransom, Paul S. Ray, Joseph D. Romano, Jessie C. Runnoe, Shashwat C. Sardesai, Ann Schmiedekamp, Carl Schmiedekamp, Kai Schmitz, Brent J. Shapiro-Albert, Xavier Siemens, Joseph Simon, Magdalena S. Siwek, Sophia V. Sosa Fiscella, Ingrid H. Stairs, Daniel R. Stinebring, Kevin Stovall, Abhimanyu Susobhanan, Joseph K. Swiggum, Stephen R. Taylor, Jacob E. Turner, Caner Unal, Michele Vallisneri, Sarah J. Vigeland, Haley M. Wahl, London Willson, Caitlin A. Witt, David Wright, Olivia Young

“…Here we investigate how signs of the discrete nature of this GWB can manifest in pulsar timing arrays (PTAs) through excursions from, and breaks in, the expected ${f}_{\mathrm{GW}}^{-2/3}$ power law of the GWB strain spectrum. …”
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The Association Between the Atherogenic Index of Plasma and Cognitive Function: Evidence From the NHANES 2011–2014 by Pingping Huang, Gaocan Ren, Yifei Wang, Yicheng Liu, Hongwei Zhang, Shuangqing Fu, Zhibo Zhang, Lijun Guo, Xiaochang Ma

“…The relationship between AIP and cognitive function was examined using several multivariate regression models with adjustment for arrays of potential confound factors. Fitted smoothed curves and threshold effect analysis were used to characterize possible nonlinear relationships. …”
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Genome-wide association for abdominal subcutaneous and visceral adipose reveals a novel locus for visceral fat in women. by Caroline S Fox, Yongmei Liu, Charles C White, Mary Feitosa, Albert V Smith, Nancy Heard-Costa, Kurt Lohman, GIANT Consortium, MAGIC Consortium, GLGC Consortium, Andrew D Johnson, Meredith C Foster, Danielle M Greenawalt, Paula Griffin, Jinghong Ding, Anne B Newman, Fran Tylavsky, Iva Miljkovic, Stephen B Kritchevsky, Lenore Launer, Melissa Garcia, Gudny Eiriksdottir, J Jeffrey Carr, Vilmunder Gudnason, Tamara B Harris, L Adrienne Cupples, Ingrid B Borecki

“…More refined phenotypes for body composition and fat distribution can detect new loci not previously uncovered in large-scale GWAS of anthropometric traits.…”
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Methylome analysis and epigenetic changes associated with menarcheal age. by Christiana A Demetriou, Jia Chen, Silvia Polidoro, Karin van Veldhoven, Cyrille Cuenin, Gianluca Campanella, Kevin Brennan, Françoise Clavel-Chapelon, Laure Dossus, Marina Kvaskoff, Dagmar Drogan, Heiner Boeing, Rudolf Kaaks, Angela Risch, Dimitrios Trichopoulos, Pagona Lagiou, Giovanna Masala, Sabina Sieri, Rosario Tumino, Salvatore Panico, J Ramón Quirós, María-José Sánchez Perez, Pilar Amiano, José María Huerta Castaño, Eva Ardanaz, Charlotte Onland-Moret, Petra Peeters, Kay-Tee Khaw, Nick Wareham, Timothy J Key, Ruth C Travis, Isabelle Romieu, Valentina Gallo, Marc Gunter, Zdenko Herceg, Kyriacos Kyriacou, Elio Riboli, James M Flanagan, Paolo Vineis

“…This study suggests a link between age at menarche and genome wide DNA methylation, and the difference in results between the two arrays suggests that repetitive element methylation has a role in the association. …”
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Testicular large B‐cell lymphoma is genetically similar to PCNSL and distinct from nodal DLBCL by Alfredo Rivas‐Delgado, Cristina López, Guillem Clot, Ferran Nadeu, Marta Grau, Gerard Frigola, Jan Bosch‐Schips, Josefine Radke, Naveed Ishaque, Miguel Alcoceba, Gustavo Tapia, Luis Luizaga, Carmen Barcena, Nicholas Kelleher, Neus Villamor, Tycho Baumann, Ana Muntañola, Juan M. Sancho‐Cia, Alejandro M. García‐Sancho, Eva Gonzalez‐Barca, Estella Matutes, Jordi A. Brito, Kennosuke Karube, Itziar Salaverria, Anna Enjuanes, Stefan Wiemann, Frank L. Heppner, Reiner Siebert, Fina Climent, Elías Campo, Eva Giné, Armando López‐Guillermo, Silvia Beà

“…BCL6 rearrangements were detected in 36% of cases, and no concomitant BCL2 and MYC rearrangements were found. …”
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Integrated cytogenetic and genomic profiling of the MDS-L cell line by Julia Mestre, Lorea Chaparro-González, Isabel Granada, Mar Mallo, Emili Cid, Estefania Mancini, Oriol Calvete, Ruth M. Risueño, Daniel T. Starczynowski, Francesc Solé

“…A comprehensive approach was employed combining conventional G banding, multicolor FISH (M-FISH), SNP arrays with the novel Optical Genome Mapping (OGM) technology. …”
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Murine leukemia virus p12 tethers the capsid-containing pre-integration complex to chromatin by binding directly to host nucleosomes in mitosis. by Madushi Wanaguru, David J Barry, Donald J Benton, Nicola J O'Reilly, Kate N Bishop

“…Direct binding of MLV p12_M63I peptides to histones was confirmed by biolayer-interferometry (BLI) assays using highly-avid recombinant poly-nucleosomal arrays. Excitingly, using this method, we also observed binding between MLV p12_WT and nucleosomes. …”
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Functional Verification of Extended Kalman Filter on FPGA for Railway Wheelset Parameters Estimation by Khakoo Mal, Tayab Din Memon, Imtiaz Hussain Kalwar, Allah Rakhio Junejo, Majid Hussain Memon, Tarique Rafique Memon, Sufyan Ali Memon, Bhawani Shankar Chowdhry

“…The fast-processing speed and hardware flexibility of field programmable gate arrays (FPGAs) make them ideal for the implementation of nonlinear condition monitoring systems. …”
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Possibilities and reserves of using electronic medical records data in health information systems on the example of assessing the causes of visits to outpatient clinics and death i... by I. V. Samorodskaya, R. N. Shepel, I. V. Klyuchnikov, M. M. Lukyanov, S. Yu. Martsevich, E. P. Kakorina, O. M. Drapkina

“…Hypertension, cerebrovascular diseases, acute cerebrovascular accident, cancer and COVID-19 were most frequently detected among patients in group 3, while diabetes was most frequently found in participants in group 2.Conclusion. …”
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