Decoding bacterial methylomes in four public health-relevant microbial species: nanopore sequencing enables reproducible analysis of DNA modifications by Valentina Galeone, Johanna Dabernig-Heinz, Mara Lohde, Christian Brandt, Christian Kohler, Gabriel E. Wagner, Martin Hölzer

“…Recent advancements in real-time Nanopore sequencing and basecalling algorithms have enabled the direct detection of modified bases from raw signal data, eliminating the need for bisulfite treatment of DNA. …”
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Exploring the approaches towards support of academic entrepreneurship: evidence from an emerging market by Marek Zinecker, Jan Pěnčík, Alena Kocmanová, Tomáš Meluzín, Adam P. Balcerzak, Michał Bernard Pietrzak

“…First, the study provides a useful methodological concept of data collection as the multi-level approach was applied for studying the phenomenon of entrepreneurial university. …”
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Exploring nurse and patient perspectives on WeChat-based prenatal education in Chinese public hospitals: a qualitative inquiry by Yushi He, Guangmei Fan, Guangrui Fan, Dandan Liu

“…Seventeen obstetric nurses and eight pregnant women participated in semi-structured interviews between March and August 2024. Data were analyzed using thematic analysis to identify commonalities and divergences between nurse-driven and patient-driven experiences. …”
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The effects of alcohol on recovery from musculoskeletal trauma and injuries by Shahrzad Bazargan-Hejazi, Vincent Arriola, Daniel Arriola, Deyu Pan, Kaveh Dehghan, Emad Alamoutifard, Elby Washington

“…These findings also highlight the potential economic implications of alcohol-related musculoskeletal injuries. …”
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Association between sarcopenic obesity and dementia in the Chinese elderly using different definitions of obesity: evidence from the CHARLS by Lang Peng, Lang Peng, Lang Peng, Qingwei Xiang, Qingwei Xiang, Qingwei Xiang, Ge Jia, Ge Jia, Ge Jia, Renyi Yin

“…When stratified by age and gender, the association between sarcopenic obesity and probable dementia remained significant.Conclusion and implicationsThe findings from this cross-sectional study suggest that both possible sarcopenia and sarcopenic obesity are significantly associated with an increased risk of probable dementia among older adults residing in the community in China. …”
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Risk Factors Associated with Hyporesponsiveness to Erythropoietin in Chronic Kidney Disease Patients on Hemodialysis Who Present Anemia: A Multicenter Case-Control Study by Carlos Perez Tulcanaza, André Benítez-Baldassari, Andrea Banegas-Sarmiento, Jose Daniel Sanchez

“…Methods: This multicenter case–control study analyzed data from 784 hemodialysis patients receiving erythropoietin therapy across six dialysis centers in Ecuador between January and December 2019. …”
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Understanding the Complexity of the Food System: Differences and Commonalities between Two Optimization Models by Samantha N. Heerschop, Renee P. M. Cardinaals, Sander Biesbroek, Argyris Kanellopoulos, Johanna M. Geleijnse, Pieter van ‘t Veer, Hannah H. E. van Zanten

“…This has resulted in models that are unique but have a similar overarching aim: ‘to create a sustainable food system by understanding the implications of food system choices’. However, the results of these models may differ. …”
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Usability testing of an individualized decision aid for total knee arthroplasty by Jeffrey A. Johnson, Ademola Joshua Itiola, Shakib Rahman, Christopher Smith, Allison Soprovich, Lisa A. Wozniak, Deborah A. Marshall

“…Eligible adults diagnosed with knee OA completed the decision aid online and subsequently filled out demographics and survey forms, including the Preparation for Decision Making Scale (PDMS), System Usability Scale (SUS), and Acceptability Scale. Data were analyzed using descriptive statistics and content analysis of open-ended responses. …”
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Multi-omics exploration of the factors influencing feather coverage in laying hens by Xin Li, Xiaohui Shen, Xiaoliang Wang, Yao Yan, Wei Liu, Kai Zhan, Daqian He, Changsuo Yang, Huaxiang Yan, Junfeng Yao

“…ObjectiveThis study aimed to elucidate the molecular and microbial mechanisms underlying feather coverage in laying hens, with implications for understanding epithelial homeostasis and potential translational applications in tissue regeneration. …”
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High protein intake causes gene-length-dependent transcriptional decline, shortens lifespan and accelerates ageing in progeroid DNA repair-deficient mice by Ivar van Galen, Maria B. Birkisdóttir, Rutger A. Ozinga, Renata M. C. Brandt, Sander Barnhoorn, Sandra Imholz, Conny T. van Oostrom, Ricfrid W. G. N. van der Marel, Kimberly Smit, Yvonne M. A. Rijksen, Erwin Reiling, Harry van Steeg, Jan H. J. Hoeijmakers, Martijn E. T. Dollé, Wilbert P. Vermeij

“…Finally, GLTD was also identified by reanalysis of publicly available data of wild-type mice, rats and humans on high-protein diets, suggesting that increased dietary protein enhances GLTD and accelerates systemic ageing. …”
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Pangolin scales as adaptations for innate immunity against pathogens by Xuechen Tian, Li Chen, Jinfeng Zhou, Enbo Wang, Mu Wang, Nicholas Jakubovics, Jing Li, Kunping Song, King Tong Lau, Klaus-Peter Koepfli, Siyuan Zhang, Geok Yuan Annie Tan, Yixin Yang, Siew Woh Choo

“…Using a multi-omics analysis approach, we report a novel functional explanation for how mammalian scales can provide host–pathogen defense. Results Our data suggest that pangolin scales have a sophisticated structure that could potentially trap pathogens. …”
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Embedding research into the organisational structure of smaller rural hospitals: building research culture and capacity and understanding perceived rural health workforce benefit by Laura Alston, Dai Pu, Sarah Wood, Michael Field, Hannah Beks, Olivia A. King, Nikita Wheaton, Catherine E. Huggins, Anna Wong Shee, Kevin Mc Namara, Vincent L. Versace

“…It also explored the association between job satisfaction and research activity among hospital staff. Data analysis included descriptive statistics and linear regression. …”
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Methuosis key gene ARF6 as a diagnostic, prognostic and immunotherapeutic marker for prostate cancer: based on a comprehensive pan-cancer multi-omics analysis by Yongjin Yang, Liangliang Qing, Chengyu You, Qingchao Li, Wenbo Xu, Zhilong Dong

“…Further experimental validation is needed to confirm these observations and to explore the therapeutic implications of targeting ARF6 in cancer treatment.…”
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Assessment of Stress Levels and Coping Strategies Among Students at a Dental School in Southern California by Dima Ibnian, Axel Barba, Sara Mojtahedi, Debra Friesen, Udochukwu Oyoyo, So Ran Kwon

“…The DES questionnaire rates 24 academic, clinical, social, and personal stressors on a five-point Likert scale (1 = not stressful to 5 = highly stressful), yielding total scores from 24 to 120. Quantitative data were analyzed using descriptive statistics, t-tests, and correlation analyses. …”
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Cambrian-Devonian paleogeographic evolution of the western and central segments of North Africa by Yilin Li, Zhiqiang Feng, Naixi Zheng, Lei Li, Zongfeng Li, Hancheng Ji, Zhidong Bao

“…Integrating seismic, well log, and core data, we identify key depositional patterns and their implications for hydrocarbon exploration. …”
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Enhancing Genomic Prediction Accuracy with a Single-Step Genomic Best Linear Unbiased Prediction Model Integrating Genome-Wide Association Study Results by Zhixu Pang, Wannian Wang, Pu Huang, Hongzhi Zhang, Siying Zhang, Pengkun Yang, Liying Qiao, Jianhua Liu, Yangyang Pan, Kaijie Yang, Wenzhong Liu

“…The single-step GBLUP (ssGBLUP) model, which integrates pedigree, phenotypic, and genomic data, has improved genomic prediction. However, ssGBLUP assumes that all markers contribute equally to genetic variance, which can limit its predictive accuracy, especially for traits controlled by major genes. …”
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Life stage and seasonal habitat use of the porbeagle Lamna nasus in the Northwest Atlantic Ocean by Brooke N. Anderson, Neil Hammerschlag, Steven Saul, Jon F. Dodd, Heather D. Bowlby, Yun Kang, Austin J. Gallagher, James A. Sulikowski

“…Research incorporating movement data across seasons and life stages, including reproductive states, is valuable for informing spatial management, yet is absent for most species. …”
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Disrupted Circadian Rhythms as a Novel Driver of Sarcopenia: Mechanisms, Evidence, and Future Directions by Alina Deniza Ciubean, Theodor Popa, Viorela Mihaela Ciortea, Gabriela Dogaru, Laszlo Irsay

“…We explore molecular mechanisms, epidemiological data, experimental studies, and emerging therapeutic strategies. …”
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Using photovoice to engage underserved children with neurodevelopmental disorders and their caregivers in health research: a mixed methods systematic review by Miriam Gonzalez, Paul Y. Yoo, Samantha E. Noyek, Brooke MacLeod, Michelle Kee, Michelle Phoenix, Samantha K. Micsinszki, Marion Knutson, Christine J. Neilson, Roberta L. Woodgate

“…We used a convergent integrated synthesis approach as well as qualitative content analysis to synthesize data from included studies.ResultsThe majority of children and caregivers in selected studies were white. …”
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THE PROBLEM OF INTERDEPARTMENTAL INTERACTION OF PUBLIC AUTHORITIES DURING CONTROL (SUPERVISORY) EVENTS by Maria S. Demina, Alexander V. Bogdashin

“…The research materials used are regulatory legal acts regulating state and municipal control (supervision), data on the functioning of digital platforms (EPER, EPNM, SMEV), as well as the practice of using digital technologies in control and supervisory activities. …”
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