Heterologous Expression of the Nitrogen-Fixing Gene Cluster from <i>Paenibacillus polymyxa</i> in <i>Bacillus subtilis</i> by Xiuling Wang, Shiqing Gao, Jun Fu, Ruijuan Li

“…However, optimizing nitrogenase activity in native biological nitrogen-fixing bacteria has been hindered by the complexities of genetic manipulation. Heterologous expression has served as a foundational strategy for engineering next-generation nitrogen-fixing microbial agents. …”
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Recovery in personality disorders: the development and preliminary testing of a novel natural language processing model to identify recovery in mental health electronic records by Giouliana Kadra-Scalzo, Jaya Chaturvedi, Oliver Dale, Richard D. Hayes, Lifang Li, Shaza Mahmood, Jonathan Monk-Cunliffe, Angus Roberts, Paul Moran

“…Yet, investigating non-symptomatic recovery in the field of personality disorders has been particularly challenging due to complexities in capturing the occurrence of recovery. …”
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U-Net-based VGG19 model for improved facial expression recognition by Xiaohu ZHAO, Jingyi ZHANG, Mingzhi JIAO, Lixun XIE, Lanfei WANG, Weiqing SUN, Di ZHANG

“…The use of feature cropping and stitching techniques allows the model to efficiently integrate multi-scale features, thereby enhancing the robustness and effectiveness of facial expression recognition tasks. …”
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Identification and Functional Testing of ERCC2 Mutations in a Multi-national Cohort of Patients with Familial Breast- and Ovarian Cancer. by Andreas Rump, Anna Benet-Pages, Steffen Schubert, Jan Dominik Kuhlmann, Ramūnas Janavičius, Eva Macháčková, Lenka Foretová, Zdenek Kleibl, Filip Lhota, Petra Zemankova, Elitza Betcheva-Krajcir, Luisa Mackenroth, Karl Hackmann, Janin Lehmann, Anke Nissen, Nataliya DiDonato, Romy Opitz, Holger Thiele, Karin Kast, Pauline Wimberger, Elke Holinski-Feder, Steffen Emmert, Evelin Schröck, Barbara Klink

“…Ten out of eleven mutations that were tested exemplarily in cell-based functional assays exert diminished excision repair efficiency and/or decreased transcriptional activation capability. …”
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Comprehensive characterization and environmental implications of industrial and hazardous incineration ashes: insights into chemistry, mineralogy, elements’ fractionation and leach... by Monika Kasina, Anna Telk, Małgorzata Wendorff-Belon

“…The complexity of mineral composition and bonds within the sample indicates the necessity for a thorough environmental assessment to understand the potential impacts on the ecosystem. …”
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Tools for forecasting regional economic growth using big data and business intelligence technologies by Afanasev Kirill, Kalinin Aleksandr

“…The significance of the study is due to the increasing complexity of regional economic growth forecasting in the context of digital transformation and the limitations of traditional analysis methods. …”
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Discriminating and classifying odontocete echolocation clicks in the Hawaiian Islands using machine learning methods. by Morgan A Ziegenhorn, Kaitlin E Frasier, John A Hildebrand, Erin M Oleson, Robin W Baird, Sean M Wiggins, Simone Baumann-Pickering

“…However, due to the size of this dataset and the complexity of species-level acoustic classification, multi-year, multi-species analyses had not yet been completed. …”
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USING THE ANYLOGIC ENVIRONMENT FOR MODELING AND ANALYSIS OF THE INFORMATION SECURITY AUDIT PROCESS by Kamilla M. Khuranova, Igor D. Kologorov, Sergey A. Reznichenko, Leonid N. Kessarinskiy

“…The relevance of the study is due to the increasing complexity of cyber threats and the need to optimize the resources spent on ensuring the security of information systems. …”
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Implementing Customized Electronic Quality Checklists to Reduce Errors and Improve Workflow in Radiotherapy by Zaheeda Mulla, Victor J. Joseph John, Rania Hashem, Hatim Z. Almarzouki, Abdullah Almohamad, Mohammad Attar, Hane Muamenah, Hanadi F. Habibullah, Hussam Hijazi

“…PURPOSEAdvancements in radiation oncology have improved treatment precision and effectiveness but increased procedural complexity and error risk because of higher patient volumes. …”
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Novel Epigenetics Control (EpC) Nanocarrier for Cancer Therapy Through Dual-Targeting Approach to DNA Methyltransferase and Ten-Eleven Translocation Enzymes by Risa Mitsuhashi, Kiyoshi Sato, Hiroyoshi Kawakami

“…The formation of core–shell nanoparticles and complexation with pDNA were investigated and optimized by dynamic light scattering, zeta potential measurement, and agarose gel electrophoresis. …”
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Improving the Principles of Identifying Critical Requirements for the Assembly of High-Precision Products by A. V. Nazaryev, P. Yu. Bochkarev

“…The application of this technique makes it possible to increase the reliability of the source information obtained during the implementation of an enlarged block of design procedures, as well as the validity and efficiency of identifying rational manufacturing technologies at subsequent stages of the system implementation, while providing the specified quality, accuracy of products, and reducing the complexity and cost of their manufacture.…”
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Truncating the spliceosomal ‘rope protein’ Prp45 results in Htz1 dependent phenotypes by Kateřina Abrhámová, Martina Groušlová, Anna Valentová, Xinxin Hao, Beidong Liu, Martin Převorovský, Ondřej Gahura, František Půta, Per Sunnerhagen, Petr Folk

“…The interaction of prp45(1–169) and htz1Δ was detectable even in cells with SRB2 intron deleted (srb2Δi). …”
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Infusing Harvest Plus lines into commercial cultivars: Impact on grain yield and micronutrient content in wheat (Triticum aestivum L.) by Ashish Sheera, Tuhina Dey, Nashra Aftab, Jogendra Singh, Vijayata Singh, Rebekah Nisha, Bikram Singh

“…The findings highlight the complexity of genetic interactions and emphasize the need for advanced breeding strategies to develop wheat varieties that improve yield and micronutrient efficiency, addressing global nutritional challenges.…”
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GNNs and ensemble models enhance the prediction of new sRNA-mRNA interactions in unseen conditions by Shani Cohen, Lior Rokach, Isana Veksler-Lublinsky

“…Despite advancements in high-throughput (HT) experimental methods, they remain technically challenging and are limited to detecting sRNA-target interactions under specific environmental conditions. …”
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Deciphering cell cycle organization of Toxoplasma endodyogeny by Mrinalini Batra, Clem Marsilia, Danya Awshah, Lauren M. Hawkins, Chengqi Wang, Dale Chaput, Daria A. Naumova, Elena S. Suvorova

“…The ToxoFUCCIS probe efficiently detected G2/M and Spindle Assembly Checkpoints, as well as the SB505124-induced TgMAPK1-dependent block. …”
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