Luciferase complementation based-detection of G-protein-coupled receptor activity by Hideaki Yano, Ning Sheng Cai, Jonathan A Javitch, Sergi Ferré

“…Methods to detect activities of G protein-coupled receptors (GPCRs) have particular relevance for drug screening. …”
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Urine complement analysis implies complement activation is involved in membranous nephropathy by Yingxue Xu, Yingxue Xu, Yi Li, Yong Zhang, Guisen Li, Guisen Li

“…BackgroundThe onset and progression of membranous nephropathy (MN) have been associated with complement activation, yet the overall characteristics of this activation in the kidneys remain unclear. …”
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Activity-based Detection of HIF stabilizers: a Future-proof Complementing Method in Doping Control? by L.K. Janssens, L. De Wilde, P. Van Eenoo, C.P. Stove

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Angiotensinogen and C3 compete for renin-induced complement activation by Ann-Charlotte Kristoffersson, Albin Sköld, Charlotte Welinder, Markus Wendler, Gabriella Kalliokoski, Zivile Bekassy, Diana Karpman

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A Cell-Based Assay to Measure the Activity of the Complement Convertases by Małgorzata Stasiłojć, Grzegorz Stasiłojć, Alicja Kuźniewska, Santiago Rodriguez de Córdoba, Marcin Okrój

“…Aberrant regulation of the convertases is often implicated in the development of rare complement-related diseases. However, analyzing convertase activity poses a significant challenge due to their labile nature and intricate interactions with serum proteins. …”
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Catastrophic antiphospholipid syndrome accompanied by complement regulatory gene mutation by Serim Pul, Betül Sözeri, Cemile Pehlivanoğlu, Deniz Filinte, Özde Nisa Türkkan, Mehtap Sak, Neslihan Çiçek, Serçin Güven, Ece Demirci Bodur, İbrahim Gökçe, Harika Alpay

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Complement system is activated in acute inflammatory response to environmental particulates in the lungs by Kerui Zhang, Akiko Honda, Tomoya Sagawa, Natsuko Miyasaka, Binyang Qiu, Raga Ishikawa, Tomoaki Okuda, Takayuki Kameda, Kaori Sadakane, Takamichi Ichinose, Hirohisa Takano

“…Short-term exposure (typically ranging from a few hours to 7 days) to particulate matter (PM) elicits acute inflammatory responses with significant activation of complement component C5. However, the relationship between PM-induced complement system activation, acute inflammation, and the contributing factors remains unclear. …”
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Africanized honeybee venom (Apis mellifera) promotes human complement activation split products storm by Felipe Silva de França, Felipe Silva de França, Ricardo de Oliveira Orsi, Dayanne Carla Fernandes, Dayanne Carla Fernandes, Thyago Bispo Leonel, Thyago Bispo Leonel, Denise V. Tambourgi, Denise V. Tambourgi

“…IntroductionComplement activation split products are signatures of many immunopathological disorders. …”
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Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System by Nadezhda Shilova, Alexey Nokel, Alexander Lipatnikov, Nailya Khasbiullina, Yuri Knirel, Ludmila Baidakova, Alexander Tuzikov, Sergei Khaidukov, Polina Obukhova, Stephen Henry, Batozhab Shoibonov, Emin Salimov, Robert Rieben, Nicolai Bovin

“…</b> It was found that about 30% of anti-glycan antibodies in human serum detected by the PGA did not activate the complement. …”
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Distinction of early complement classical and lectin pathway activation via quantification of C1s/C1-INH and MASP-1/C1-INH complexes using novel ELISAs by Lisa Hurler, Erik J. M. Toonen, Erika Kajdácsi, Bregje van Bree, Ricardo J. M. G. E. Brandwijk, Wieke de Bruin, Paul A. Lyons, Paul A. Lyons, Laura Bergamaschi, Laura Bergamaschi, Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration, György Sinkovits, László Cervenak, Reinhard Würzner, Zoltán Prohászka, Zoltán Prohászka, Stephen Baker, John R. Bradley, Patrick F. Chinnery, Daniel J. Cooper, Gordon Dougan, Ian G. Goodfellow, Ravindra K. Gupta, Nathalie Kingston, Paul J. Lehner, Paul A. Lyons, Nicholas J. Matheson, Caroline Saunders, Kenneth G. C. Smith, Charlotte Summers, James Thaventhiran, M. Estee Torok, Mark R. Toshner, Michael P. Weekes, Gisele Alvio, Sharon Baker, Areti Bermperi, Karen Brookes, Ashlea Bucke, Jo Calder, Laura Canna, Cherry Crucusio, Isabel Cruz, Rnalie de Jesus, Katie Dempsey, Giovanni Di Stephano, Jason Domingo, Anne Elmer, Julie Harris, Sarah Hewitt, Heather Jones, Sherly Jose, Jane Kennet, Yvonne King, Jenny Kourampa, Emily Li, Caroline McMahon, Anne Meadows, Vivien Mendoza, Criona O&#x2019;Brien, Charmain Ocaya, Ciro Pascuale, Marlyn Perales, Jane Price, Rebecca Rastall, Carla Ribeiro, Jane Rowlands, Valentina Ruffolo, Hugo Tordesillas, Phoebe Vargas, Bensi Vergese, Laura Watson, Jieniean Worsley, Julie-Ann Zerrudo, Laura Bergamaschi, Ariana Betancourt, Georgie Bower, Ben Bullman, Chiara Cossetti, Aloka De Sa, Benjamin J. Dunore, Maddie Epping, Stuart Fawke, Stefan Gr&#xe4;f, Richard Grenfell, Andrew Hinch, Josh Hodgson, Christopher Huang, Oisin Huhn, Kelvin Hunter, Isobel Jarvis, Emma Jones, Ma&#x161;a Josipovi&#x107;, Ekaterina Legchenko, Daniel Lewis, Joe Marsden, Jennifer Martin, Federica Mescia, Francesca Nice, Ciara O&#x2019;Donnell, Ommar Omarjee, Marianne Perera, Linda Pointon, Nicole Pond, Nathan Richoz, Nika Romashova, Natalia Savoinykh, Rahul Sharma, Joy Shih, Mateusz Strezlecki, Rachel Sutcliffe, Tobias Tilly, Zhen Tong, Carmen Treacy, Lori Turner, Jennifer Wood, Marta Wylot, John Allison, Heather Biggs, Helen Butcher, Daniela Caputo, Debbie Clapham-Riley, Eleanor Dewhurst, Christian Fernandez, Anita Furlong, Barbara Graves, Jennifer Gray, Tasmin Ivers, Emma Le Gresley, Rachel Linger, Mary Kasanicki, Sarah Meloy, Francesca Muldoon, Nigel Ovington, Sofia Papadia, Christopher J. Penkett, Isabel Phelan, Venkatesh Ranganath, Jennifer Sambrook, Katherine Schon, Hannah Stark, Kathleen E. Stirrups, Paul Townsend, Julie von Ziegenweidt, Jennifer Webster, Ali Asaripour, Lucy Mwaura, Caroline Patterson, Gary Polwarth, Katherine Bunclark, Michael Mackay, Alice Michael, Sabrina Rossi, Mayurun Selvan, Sarah Spencer, Cissy Yong, Petra Polgarova

“…Measurement of the complexes reveals information about the involvement of the respective pathways in complement-mediated diseases. Two sandwich ELISAs detecting C1s/C1-INH and MASP-1/C1-INH complex were developed and tested thoroughly, and it was investigated whether C1s/C1-INH and MASP-1/C1-INH complexes could serve as markers for either early CP or LP activation. …”
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Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies by Yang Yang, Ying Zhang, Yuan Li, Xinjin Zhou, Kazuho Honda, Dedong Kang, Muxi Wang, Jing-Hua Yang, Zongping Xia, Yuan Wei, Lu Liu, Ruimin Hu, Takashi Takaki, Guolan Xing

“…Abstract Increasing evidence points toward an essential role for complement activation in the pathogenesis of diabetic kidney disease (DKD). …”
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Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation by Jesper Karlsson, Lina Wirestam, Hanna Duàn, Suhana Ahmad, Daniel Appelgren, Helena Enocsson, Jonas Wetterö, Christopher Sjöwall

“…Furthermore, we show that patients with anticardiolipin IgG and/or anti-β2GPI IgG antibodies have a higher number of positive CRP epitopes, and some CRP autoantibody-specificities associate with antiphospholipid antibodies, disease activity, and classical complement activation. In addition, one identified motif was selected, synthesized, and used for studying neutrophil function. …”
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A novel antibody against human properdin inhibits the alternative complement system and specifically detects properdin from blood samples. by Diana Pauly, Benedikt M Nagel, Jörg Reinders, Tobias Killian, Matthias Wulf, Susanne Ackermann, Boris Ehrenstein, Peter F Zipfel, Christine Skerka, Bernhard H F Weber

“…Here, we generated a novel blocking monoclonal antibody (mAb) against properdin and devised a new diagnostic assay for this important complement regulator. Mouse mAb 1340 specifically detected native properdin from human samples with high avidity. …”
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SARS-CoV-2 Protein Deposition Enhances Renal Complement Activation and Aggravates Kidney Injury in Membranous Nephropathy After COVID-19 by Guoqin Wang, Lei Yang, Xiaoyi Xu, Weiyi Guo, Lijun Sun, Yanyan Wang, Wenrong Cheng, Nan Ye, Lingqiang Kong, Xiaoyi Zhao, Hong Cheng

“…The clinicopathological characteristics and complement system activation of MN after COVID-19 are unclear. …”
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Multi-centered clinical validation demonstrating superior precision in lupus diagnosis: T cell autoantibodies and TC4d outperform conventional lupus erythematosus biomarkers by Vasileios Kyttaris, Daniel J. Wallace, Arezou Khosroshahi, Andrew Concoff, Nicole Wilson, Chau Ching Liu, Susan Manzi, Joseph Ahearn, Sepehr Taghavi, Touba Warsi, Stanley Park, Christine Schleif, Brittany D. Partain, Tyler O’Malley

“…Semi-quantitative flow cytometry analysis included TIgG and TIgM autoantibodies, cell-bound complement activation products (CB-CAPs), TC4d, erythrocyte-bound C4d (EC4d) and B lymphocyte-bound C4d (BC4d). …”
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DYSFUNCTION OF COMPLEMENT SYSTEM IN FAMILIAL MEDITERRANEAN FEVER by G. M. Mkrtchyan, L. P. Hovhannisyan, A. A. Ayvazyan, E. Y. Nazaretyan, A. S. Boyajyan

“…In present study, we examined functional activities of the alternative and the classical complement cascades, and some relationships between alterations in the functional activities of these cascades in FMF. …”
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Analysis of human factor H-related gene and protein expressed in rheumatoid arthritis synovium identifies a novel mechanism promoting dysregulated complement pathway activation by Nirmal K. Banda, Larry W. Moreland, Kevin D. Deane, Dmitri Simberg, Robert I. Scheinman, Rachel M. Frank, Jennifer A. Seifert, Accelerating Medicines Partnership (AMP) RA/SLE Network, Rachel Lau, Costantino Pitzalis, Myles J. Lewis, V. Michael Holers

“…Abstract Factor H (FH) is a negative regulator of the alternative pathway (AP) of complement however, five human factor H-related (FHR) proteins, can also function ex vivo as positive regulators. …”
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A distinct variant of the SzM protein of Streptococcus equi subsp. zooepidemicus recruits C1q independent of IgG binding and inhibits activation of the classical complement pathway by René Bergmann, Wieland Schroedl, Uwe Müller, Christoph Georg Baums

“…Addition of human plasma to immobilized rSzM_C2 and immobilized aggregated IgG led to binding of C1q, but only the latter activated the complement system, as shown by the detection of C4 deposition. …”
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Dimerization of ABCG2 analysed by bimolecular fluorescence complementation. by Ameena J Haider, Deborah Briggs, Tim J Self, Hannah L Chilvers, Nicholas D Holliday, Ian D Kerr

“…Point mutations in ABCG2 which may affect dimer formation were examined for alterations in the magnitude of fluorescence complementation signal. Bimolecular fluorescence complementation (BiFC) demonstrated specific ABCG2 dimer formation, but no changes in dimer formation, resulting from single amino acid substitutions, were detected by BiFC analysis.…”
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Improving detectability of illegal fishing activities across supply chains by Rodrigo Oyanedel, Stefan Gelcich, E. J. Milner-Gulland, Chris Wilcox

“…Abstract Improving detectability (i.e., enforcers’ capacity to detect illegal fishing activities) is vital for fisheries management, food security, and livelihoods. …”
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