Luciferase complementation based-detection of G-protein-coupled receptor activity by Hideaki Yano, Ning Sheng Cai, Jonathan A Javitch, Sergi Ferré

“…Methods to detect activities of G protein-coupled receptors (GPCRs) have particular relevance for drug screening. …”
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Urine complement analysis implies complement activation is involved in membranous nephropathy by Yingxue Xu, Yingxue Xu, Yi Li, Yong Zhang, Guisen Li, Guisen Li

“…BackgroundThe onset and progression of membranous nephropathy (MN) have been associated with complement activation, yet the overall characteristics of this activation in the kidneys remain unclear. …”
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Activity-based Detection of HIF stabilizers: a Future-proof Complementing Method in Doping Control? by L.K. Janssens, L. De Wilde, P. Van Eenoo, C.P. Stove

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Angiotensinogen and C3 compete for renin-induced complement activation by Ann-Charlotte Kristoffersson, Albin Sköld, Charlotte Welinder, Markus Wendler, Gabriella Kalliokoski, Zivile Bekassy, Diana Karpman

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Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases by Karolin Wellner, Josefine Gnauck, Dorian Bernier, Stephan H. Bernhart, Heike Betat, Mario Mörl

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Zinc supplementation inhibits complement activation in age-related macular degeneration. by Dzenita Smailhodzic, Freekje van Asten, Anna M Blom, Frida C Mohlin, Anneke I den Hollander, Johannes P H van de Ven, Ramon A C van Huet, Joannes M M Groenewoud, Yuan Tian, Tos T J M Berendschot, Yara T E Lechanteur, Sascha Fauser, Chris de Bruijn, Mohamed R Daha, Gert Jan van der Wilt, Carel B Hoyng, B Jeroen Klevering

“…Furthermore, we investigated the effect of zinc on complement activation in vitro. AMD patients with high levels of complement catabolism at baseline exhibited a steeper decline in serum complement activation (p<0.001) during the three month zinc supplementation period compared to patients with low complement levels. …”
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A Cell-Based Assay to Measure the Activity of the Complement Convertases by Małgorzata Stasiłojć, Grzegorz Stasiłojć, Alicja Kuźniewska, Santiago Rodriguez de Córdoba, Marcin Okrój

“…Aberrant regulation of the convertases is often implicated in the development of rare complement-related diseases. However, analyzing convertase activity poses a significant challenge due to their labile nature and intricate interactions with serum proteins. …”
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Association between circulatory complement activation and hypertensive renal damage: a case–control study by Zhongli Wang, Tingting Zhang, Xinyu Wang, Jianlong Zhai, Lili He, Sai Ma, Qingjuan Zuo, Guorui Zhang, Yifang Guo

“…Objective The aim of this study was to investigate the potential importance of complement system activation, with particular emphasis on the complement alternative pathway (AP), in the pathogenesis of hypertensive renal damage.Methods Serum complement C3, complement Factor H (CFH) and AP activation were assessed in 66 participants with established essential hypertension with renal damage (RD). …”
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Catastrophic antiphospholipid syndrome accompanied by complement regulatory gene mutation by Serim Pul, Betül Sözeri, Cemile Pehlivanoğlu, Deniz Filinte, Özde Nisa Türkkan, Mehtap Sak, Neslihan Çiçek, Serçin Güven, Ece Demirci Bodur, İbrahim Gökçe, Harika Alpay

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Complement system is activated in acute inflammatory response to environmental particulates in the lungs by Kerui Zhang, Akiko Honda, Tomoya Sagawa, Natsuko Miyasaka, Binyang Qiu, Raga Ishikawa, Tomoaki Okuda, Takayuki Kameda, Kaori Sadakane, Takamichi Ichinose, Hirohisa Takano

“…Short-term exposure (typically ranging from a few hours to 7 days) to particulate matter (PM) elicits acute inflammatory responses with significant activation of complement component C5. However, the relationship between PM-induced complement system activation, acute inflammation, and the contributing factors remains unclear. …”
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Africanized honeybee venom (Apis mellifera) promotes human complement activation split products storm by Felipe Silva de França, Felipe Silva de França, Ricardo de Oliveira Orsi, Dayanne Carla Fernandes, Dayanne Carla Fernandes, Thyago Bispo Leonel, Thyago Bispo Leonel, Denise V. Tambourgi, Denise V. Tambourgi

“…IntroductionComplement activation split products are signatures of many immunopathological disorders. …”
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Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System by Nadezhda Shilova, Alexey Nokel, Alexander Lipatnikov, Nailya Khasbiullina, Yuri Knirel, Ludmila Baidakova, Alexander Tuzikov, Sergei Khaidukov, Polina Obukhova, Stephen Henry, Batozhab Shoibonov, Emin Salimov, Robert Rieben, Nicolai Bovin

“…</b> It was found that about 30% of anti-glycan antibodies in human serum detected by the PGA did not activate the complement. …”
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Unraveling the CDK9/PP2A/ERK Network in Transcriptional Pause Release and Complement Activation in KRAS‐mutant Cancers by Yafang Wang, Lansong Xu, Lijun Ling, Mingyue Yao, Shangxuan Shi, Chengcheng Yu, Yingnian Li, Jie Shen, Hualiang Jiang, Chengying Xie

“…Mechanistically, CDK9 inhibition leads to compensatory activation of ERK‐MYC signaling, accompanied by the recovery of proto‐oncogenes, upregulation of immediate early genes (IEGs), stimulation of the complement C1r‐C3‐C3a cascade, and induction of tumor immunosuppression. …”
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Systematic Review of the Link Between Oxford MEST-C Classification and Complement Activation in IgA Nephropathy by Gabriel Ștefan, Eric Alamartine, Christophe Mariat, Nicolas Maillard

“…Methods: We adhered to the Preferred Reporting Items for Systematic Review and Meta-analysis guidelines and conducted a systematic review, utilizing databases like MEDLINE (PubMed), Embase, Scopus, and Cochrane from January 2016 (year of updated MEST-C classification) to January 2023. We specifically selected studies that employed established methods to evaluate complement activation and the MEST-C classification. …”
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Prediction of activity of low-molecular inhibitors of the classic complement pathway using computational screening approach by D. M. Karlinsky, A. P. Kaplun, M. E. Popov

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Distinction of early complement classical and lectin pathway activation via quantification of C1s/C1-INH and MASP-1/C1-INH complexes using novel ELISAs by Lisa Hurler, Erik J. M. Toonen, Erika Kajdácsi, Bregje van Bree, Ricardo J. M. G. E. Brandwijk, Wieke de Bruin, Paul A. Lyons, Paul A. Lyons, Laura Bergamaschi, Laura Bergamaschi, Cambridge Institute of Therapeutic Immunology and Infectious Disease-National Institute of Health Research (CITIID-NIHR) COVID BioResource Collaboration, György Sinkovits, László Cervenak, Reinhard Würzner, Zoltán Prohászka, Zoltán Prohászka, Stephen Baker, John R. Bradley, Patrick F. Chinnery, Daniel J. Cooper, Gordon Dougan, Ian G. Goodfellow, Ravindra K. Gupta, Nathalie Kingston, Paul J. Lehner, Paul A. Lyons, Nicholas J. Matheson, Caroline Saunders, Kenneth G. C. Smith, Charlotte Summers, James Thaventhiran, M. Estee Torok, Mark R. Toshner, Michael P. Weekes, Gisele Alvio, Sharon Baker, Areti Bermperi, Karen Brookes, Ashlea Bucke, Jo Calder, Laura Canna, Cherry Crucusio, Isabel Cruz, Rnalie de Jesus, Katie Dempsey, Giovanni Di Stephano, Jason Domingo, Anne Elmer, Julie Harris, Sarah Hewitt, Heather Jones, Sherly Jose, Jane Kennet, Yvonne King, Jenny Kourampa, Emily Li, Caroline McMahon, Anne Meadows, Vivien Mendoza, Criona O&#x2019;Brien, Charmain Ocaya, Ciro Pascuale, Marlyn Perales, Jane Price, Rebecca Rastall, Carla Ribeiro, Jane Rowlands, Valentina Ruffolo, Hugo Tordesillas, Phoebe Vargas, Bensi Vergese, Laura Watson, Jieniean Worsley, Julie-Ann Zerrudo, Laura Bergamaschi, Ariana Betancourt, Georgie Bower, Ben Bullman, Chiara Cossetti, Aloka De Sa, Benjamin J. Dunore, Maddie Epping, Stuart Fawke, Stefan Gr&#xe4;f, Richard Grenfell, Andrew Hinch, Josh Hodgson, Christopher Huang, Oisin Huhn, Kelvin Hunter, Isobel Jarvis, Emma Jones, Ma&#x161;a Josipovi&#x107;, Ekaterina Legchenko, Daniel Lewis, Joe Marsden, Jennifer Martin, Federica Mescia, Francesca Nice, Ciara O&#x2019;Donnell, Ommar Omarjee, Marianne Perera, Linda Pointon, Nicole Pond, Nathan Richoz, Nika Romashova, Natalia Savoinykh, Rahul Sharma, Joy Shih, Mateusz Strezlecki, Rachel Sutcliffe, Tobias Tilly, Zhen Tong, Carmen Treacy, Lori Turner, Jennifer Wood, Marta Wylot, John Allison, Heather Biggs, Helen Butcher, Daniela Caputo, Debbie Clapham-Riley, Eleanor Dewhurst, Christian Fernandez, Anita Furlong, Barbara Graves, Jennifer Gray, Tasmin Ivers, Emma Le Gresley, Rachel Linger, Mary Kasanicki, Sarah Meloy, Francesca Muldoon, Nigel Ovington, Sofia Papadia, Christopher J. Penkett, Isabel Phelan, Venkatesh Ranganath, Jennifer Sambrook, Katherine Schon, Hannah Stark, Kathleen E. Stirrups, Paul Townsend, Julie von Ziegenweidt, Jennifer Webster, Ali Asaripour, Lucy Mwaura, Caroline Patterson, Gary Polwarth, Katherine Bunclark, Michael Mackay, Alice Michael, Sabrina Rossi, Mayurun Selvan, Sarah Spencer, Cissy Yong, Petra Polgarova

“…Measurement of the complexes reveals information about the involvement of the respective pathways in complement-mediated diseases. Two sandwich ELISAs detecting C1s/C1-INH and MASP-1/C1-INH complex were developed and tested thoroughly, and it was investigated whether C1s/C1-INH and MASP-1/C1-INH complexes could serve as markers for either early CP or LP activation. …”
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Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies by Yang Yang, Ying Zhang, Yuan Li, Xinjin Zhou, Kazuho Honda, Dedong Kang, Muxi Wang, Jing-Hua Yang, Zongping Xia, Yuan Wei, Lu Liu, Ruimin Hu, Takashi Takaki, Guolan Xing

“…Abstract Increasing evidence points toward an essential role for complement activation in the pathogenesis of diabetic kidney disease (DKD). …”
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Complement and Coagulation Cascade Activation Regulates the Early Inflammatory Mechanism of Resistance of Suckling Lambs Against <i>Haemonchus contortus</i> by José Gabriel G. Lins, Alessandro F. T. Amarante

“…Our data identified potential markers for resistance trait selection in sheep, such as C3 (complement C3), F3 (tissue factor), F5 (coagulation factor V), CFB (complement factor B), and CFI (complement factor I). …”
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C3d-targeted complement inhibitors to correct complement dysregulation in aHUS patients by Valeria Guaschino, Donata Santarsiero, Sara Gastoldi, Joshua M. Thurman, V. Michael Holers, Shelia M. Violette, Fei Liu, Kelly C. Fahnoe, Chiara Guarinoni, Ariela Benigni, Giuseppe Remuzzi, Marina Noris, Sistiana Aiello

“…Atypical hemolytic uremic syndrome (aHUS) is a rare and severe thrombotic microangiopathy caused by genetic or acquired abnormalities leading to activation of the complement alternative pathway on cell surfaces. …”
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Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation by Jesper Karlsson, Lina Wirestam, Hanna Duàn, Suhana Ahmad, Daniel Appelgren, Helena Enocsson, Jonas Wetterö, Christopher Sjöwall

“…Furthermore, we show that patients with anticardiolipin IgG and/or anti-β2GPI IgG antibodies have a higher number of positive CRP epitopes, and some CRP autoantibody-specificities associate with antiphospholipid antibodies, disease activity, and classical complement activation. In addition, one identified motif was selected, synthesized, and used for studying neutrophil function. …”
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