Multiplex PCR and Next Generation Sequencing for the Non-Invasive Detection of Bladder Cancer. by Douglas G Ward, Laura Baxter, Naheema S Gordon, Sascha Ott, Richard S Savage, Andrew D Beggs, Jonathan D James, Jennifer Lickiss, Shaun Green, Yvonne Wallis, Wenbin Wei, Nicholas D James, Maurice P Zeegers, K K Cheng, Glenn M Mathews, Prashant Patel, Michael Griffiths, Richard T Bryan

“…<h4>Results</h4>Despite the small quantities of DNA extracted from some urine cell pellets, 96% of the samples yielded mean read depths >500. …”
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Complete chloroplast genomes of 25 mulberry plants: insight into genome characteristics, comparative analysis and phylogenetic relationships by Jinhong Yang, Wei Jiang, Jiayue Huang, Yunwu Peng, Fang Yang, Weiqing Kong

“…Results The cp. genome sequences of 25 Morus plants were determined to be 159,008–159,346 bp in length, which included a pair of inverted repeats of 25,654, 25,678 or 25,690 bp that separated a large single copy region of 87,912–88,142 bp and a small single copy region of 19,740–19,853 bp. Annotation showed that they contained a total of 129 genes: 84 protein-coding, 37 tRNA and eight rRNA genes. …”
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Parasite-derived microRNAs in host serum as novel biomarkers of helminth infection. by Anna M Hoy, Rachel J Lundie, Alasdair Ivens, Juan F Quintana, Norman Nausch, Thorsten Forster, Frances Jones, Narcis B Kabatereine, David W Dunne, Francisca Mutapi, Andrew S Macdonald, Amy H Buck

“…<h4>Background</h4>MicroRNAs (miRNAs) are a class of short non-coding RNA that play important roles in disease processes in animals and are present in a highly stable cell-free form in body fluids. …”
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Novel pathogenic variant in a mild case of type B molybdenum cofactor deficiency: case report and literature review by Morgan Kinsinger, Jelena Ivanisevic, Divakar S. Mithal

“…The MOCS2 gene is bicistronic, encoding the small (MOCS2A) and large (MOCS2B) subunits with an overlapping coding region. …”
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Optimization of bike-sharing repositioning operations: A reactive real-time approach by Enrique Jiménez-Meroño, Francesc Soriguera

“…The proposed method is conceptually simple, less dependent on demand predictions, easily implementable in any coding language and applicable to large systems at a low computational cost. …”
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SEMA3A, a gene involved in axonal pathfinding, is mutated in patients with Kallmann syndrome. by Naresh Kumar Hanchate, Paolo Giacobini, Pierre Lhuillier, Jyoti Parkash, Cécile Espy, Corinne Fouveaut, Chrystel Leroy, Stéphanie Baron, Céline Campagne, Charlotte Vanacker, Francis Collier, Corinne Cruaud, Vincent Meyer, Alfons García-Piñero, Didier Dewailly, Christine Cortet-Rudelli, Ksenija Gersak, Chantal Metz, Gérard Chabrier, Michel Pugeat, Jacques Young, Jean-Pierre Hardelin, Vincent Prevot, Catherine Dodé

“…We thus screened 386 KS patients for the presence of mutations in SEMA3A (by Sanger sequencing of all 17 coding exons and flanking splice sites) and identified nonsynonymous mutations in 24 patients, specifically, a frameshifting small deletion (D538fsX31) and seven different missense mutations (R66W, N153S, I400V, V435I, T688A, R730Q, R733H). …”
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Comparative efficacy of seven psychotherapeutic interventions for patients with depression: a network meta-analysis. by Jürgen Barth, Thomas Munder, Heike Gerger, Eveline Nüesch, Sven Trelle, Hansjörg Znoj, Peter Jüni, Pim Cuijpers

“…We identified 198 studies, including 15,118 adult patients with depression, and coded moderator variables. Each of the seven psychotherapeutic interventions was superior to a waitlist control condition with moderate to large effects (range d = -0.62 to d = -0.92). …”
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Rapid evolution of virulence and drug resistance in the emerging zoonotic pathogen Streptococcus suis. by Matthew T G Holden, Heidi Hauser, Mandy Sanders, Thi Hoa Ngo, Inna Cherevach, Ann Cronin, Ian Goodhead, Karen Mungall, Michael A Quail, Claire Price, Ester Rabbinowitsch, Sarah Sharp, Nicholas J Croucher, Tran Bich Chieu, Nguyen Thi Hoang Mai, To Song Diep, Nguyen Tran Chinh, Michael Kehoe, James A Leigh, Philip N Ward, Christopher G Dowson, Adrian M Whatmore, Neil Chanter, Pernille Iversen, Marcelo Gottschalk, Josh D Slater, Hilde E Smith, Brian G Spratt, Jianguo Xu, Changyun Ye, Stephen Bentley, Barclay G Barrell, Constance Schultsz, Duncan J Maskell, Julian Parkhill

“…Carried in these regions are coding sequences associated with drug resistance. …”
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Hyper CLS-Data-Based Robotic Interface and Its Application to Intelligent Peg-in-Hole Task Robot Incorporating a CNN Model for Defect Detection by Fusaomi Nagata, Ryoma Abe, Shingo Sakata, Keigo Watanabe, Maki K. Habib

“…The effectiveness and validity of the proposed system are demonstrated through a cooperative pick and place task using three small-sized industrial robot MG400s and a peg-in-hole task while checking undesirable defects in workpieces with a CNN model without using any programmable logic controller (PLC). …”
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Depressive symptoms in adults in rural and urban regions of Canada: a cross-sectional analysis of the Canadian Longitudinal Study on Aging by Verena Menec, Philip D St John, Robert Tate, Nancy E Newall, Denise Cloutier, Megan O'Connell

“…Data were analysed and results were obtained in 2020.Participants 21 241 adults aged 45–85.Measures Rurality was grouped as urban (n=11 772); peri-urban (n=2637); mixed (n=2125; postal codes with both rural and urban areas); and rural (n=4707). …”
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A Patient-Oriented Implementation Strategy for a Perioperative mHealth Intervention: Feasibility Cohort Study by Daan Toben, Astrid de Wind, Eva van der Meij, Judith A F Huirne, Johannes R Anema

“…Descriptive analysis and open coding were used to analyze the data. …”
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A phoenix in the greenhouse: characterization and phylogenomics of complete chloroplast genomes sheds light on the putatively extinct-in-the-wild Solanum ensifolium (Solanaceae) by Matthew R. Graham, Noorpreet Kaur, Cynthia S. Jones, Kurt Lamour, Bryan A. Connolly

“…Results The S. ensifolium chloroplast genome (155,295 bp) exhibits a typical quadripartite structure and encodes 151 genes, including 95 protein-coding genes involved in photosynthesis, transcription, translation, and other essential cellular functions. …”
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Effect of Teacher-Pupil Ratio on Academic Performance of Pupils in Selected Primary Schools in Kabale Municipality. by Turyahabwe, Frank

“…Data collected was edited, coded, entered into the computer, and analyzed with Excel, a Microsoft statistical package, the results were presented in tables. …”
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Credit Policy and Loan Recovery Among SACCOs In Kabale Municipality: A Case Study of Lyamujungu SACCO Kabale Municipality. by Atuheire, Dickson

“…Data collected was edited, coded, entered into the computer, and analyzed with Excel, a Microsoft statistical package, the results were presented in tables. …”
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Exploring the Role of Social Workers in Addressing Health Disparities Among Maginalised Population in Kabale District. by Asasira, Precious

“…Data collected was edited, coded, entered into the computer, and analyzed with Excel, a Microsoft statistical package, the results were presented in tables. …”
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Potential confounding mutations in Keio knockout strains: implications for research accuracy by Oishi Sen, Xianghui Liu, Staffan Kjelleberg, Scott A. Rice, Thomas Seviour

“…The current study provides a general overview of potential single nucleotide polymorphisms (SNPs), insertion–deletion of bases (≤50 nucleotides, INDELs) in the genome of a set of 21 knock-out mutants of the Keio collection in comparison to the parent strain. A small number of SNPs and INDELs were predicted in the coding and intergenic regions of the knock-out mutants in comparison to the parental strain through sequencing and bioinformatic analysis. …”
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The Pleiotropic Effect of ANRIL in Glaucoma and Cardiovascular Disease by Luke O’Brien, Daire J. Hurley, Michael O’Leary, Liam Bourke, Colm O’Brien

“…Targeting ANRIL therapeutically poses challenges due to its non-coding nature, but emerging RNA-based therapies, including antisense oligonucleotides and small-molecule modulators, hold promise. …”
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Expression regulation network in papillae of sea cucumbers: Whole-transcriptome and DNA methylation datasets by Wenhong Mai, Jingxian Sun, Bingyu Ning, Tanjun Zhao, Haochen Yang, Liyuan Ren, Zengdong Wang, Cao Cheng, Ange Zou, Yaoyao Zhan, Yaqing Chang

“…A total of 3,188 ceRNA networks were also identified including 3,081 long non-coding RNAs (lncRNA)/microRNAs (miRNA)/mRNA networks and 107 circular RNA (circRNA)/miRNA/mRNA networks. …”
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Morphological characteristics and systematic position of Allium guanxianense, an endemic species from Sichuan, China by Yuan Wang, Deng-Feng Xie, Bo-Ni Song, Lian Chen, Yu-Lin Xiao, Xing-Jin He

“…The cp genome spans 152,480 bp and comprises a large single copy region (82,305 bp, 53.98% length ratio), a small single copy region (18,353 bp, 12.04%), and two inverted repeat sequences (25,911 bp, 16.99%). …”
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Evidence to Support the Collaboration of SP1, MYC, and HIF1A and Their Association with microRNAs by Jong Ho Chun, Kotohiko Kimura, Monika Rajput, Ming-Hua Hsu, Yu-Chuan Liang, Akanksha Ramadas Shanbhag, Pei-Ju Chiang, Tiffany L. B. Jackson, Ru Chih C. Huang

“…In LN229 and U87MG glioblastoma cells, either tetra-methyl-O-nordihydroguaiaretic acid (M₄N) or tetra-acetyl-O-nordihydroguaiaretic acid (A₄N) suppressed SP1 and only a few stem cell-related proteins and induced only a small amount of cell death; in contrast, the combination treatment of M₄N with A₄N greatly suppressed the expression of SP1, MYC, and HIF1A, as well as all of the stem cell-related proteins examined, and greatly induced cell death. …”
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