A practical protocol for correlative confocal fluorescence and transmission electron microscopy characterization of extracellular vesicles by Juan M. Martínez-Andrade, Daniel Salgado-Bautista, Kendra Ramirez-Acosta, Ruben Darío Cadena-Nava, Meritxell Riquelme

“…This approach provides a reliable, versatile tool for studying nanoscale biological structures, with broad applications in cellular biology, nanomedicine, and related research fields.…”
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Non-walled spherical Acinetobacter baumannii is an important type of persister upon β-lactam antibiotic treatment by Jin Zou, Si-Hoi Kou, Ruiqiang Xie, Michael S. VanNieuwenhze, Jiuxin Qu, Bo Peng, Jun Zheng

“…Maintaining a dormant state to prevent antibiotics from taking effect is believed to be the fundamental mechanistic basis, and persisters normally maintain an intact cellular structure. Here we examined the morphologies of persisters in Acinetobacter baumannii survived from the treatment by three major classes of antibiotics (i.e. β-lactam, aminoglycoside, and fluoroquinolone) with microcopy and found that a fraction of enlarged spherical bacteria constitutes a major sub-population of bacterial survivors from β-lactam antibiotic treatment, whereas survivors from the treatment of aminoglycoside and fluoroquinolone were less changed morphologically. …”
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The disordered p53 transactivation domain is the target of FOXO4 and the senolytic compound FOXO4-DRI by Benjamin Bourgeois, Emil Spreitzer, Daniel Platero-Rochart, Margret Paar, Qishun Zhou, Sinem Usluer, Peter L. J. de Keizer, Boudewijn M. T. Burgering, Pedro A. Sánchez-Murcia, Tobias Madl

“…Here, we solve the solution NMR structural models of the p53 transactivation domain in complex with the FOXO4 forkhead domain and in complex with FOXO4-DRI. …”
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Matrix-induced nuclear remodeling and mechano-therapeutics by Jung-Hwan Lee, Yeo Gyun Yun, Hae-Won Kim

“…Summary: The extracellular matrix (ECM) provides structural support and mechanical cues that profoundly influence cellular behavior via nuclear mechanotransduction. …”
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Arthroscopic Hip Labral Reconstruction With Fresh Meniscal Allograft by Julia J. Beyer, M.D., Jarod A. Richards, M.D., David R. Woodard, M.D., James L. Cook, D.V.M., Ph.D., Brett D. Crist, M.D., Steven F. DeFroda, M.D., M. Eng.

“…Meniscal allograft has been considered an option for labral reconstruction due to its structural and functional similarities to the native labrum. …”
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Kinetic regulation of kinesin’s two motor domains coordinates its stepping along microtubules by Yamato Niitani, Kohei Matsuzaki, Erik Jonsson, Ronald D Vale, Michio Tomishige

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Influence of Different Cationic Polymer-Based Micelles on the Corneal Behavior and Anti-Cataract Effect of Diosmetin by Jing Zhang, Min Zha, Anping Wan, Satya Siva Kishan Yalamarty, Nina Filipczak, Xiang Li

“…C-M-T displayed the highest fluorescence signals, with a cellular internalization 3.2 times greater than that of the solution group. …”
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In Entamoeba histolytica, a BspA family protein is required for chemotaxis toward tumour necrosis factor by Anne Silvestre, Aurélie Plaze, Patricia Berthon, Roman Thibeaux, Nancy Guillen, Elisabeth Labruyère

“…Proteome screening with the TNFR1 sequence revealed a BspA family protein in E. histolytica that carries a TNFR signature domain and six leucine-rich repeats (named here as "cell surface protein", CSP, in view of its cellular location). Cell surface protein shares structural homologies with Toll-Like receptors, colocalizes with TNF and is internalized in TNF-containing vesicles. …”
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Selected Trace Elements and Their Impact on Redox Homeostasis in Eye Health by Joanna Wróblewska, Jarosław Nuszkiewicz, Marcin Wróblewski, Weronika Wróblewska, Alina Woźniak

“…Oxidative stress plays a crucial role in the pathogenesis of various ocular degenerative diseases, leading to structural and functional changes in eye tissues. This imbalance between reactive oxygen species (ROS) and antioxidants significantly contributes to conditions such as age-related macular degeneration, diabetic retinopathy, cataracts, and glaucoma. …”
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Effects of High Pressure on In Vitro Bioavailability of Curcumin Loaded in Whey Protein Isolate/Carrageenan Composite Emulsion Gel: In Vitro Digestion Coupled with Cell Culture Mod... by Jiayue Zhao, Xinmeng Zhang, Yanan Huang, Yan Tan, Shuang Ren, Fang Yuan

“…Scanning electron microscopy images revealed that higher pressures induced the formation of a more uniform and dense network structure in the gel. While the gel network structures were well-preserved after gastric digestion, they were disrupted into smaller particles following intestinal digestion, with particle size increasing with higher applied pressures. …”
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Insights into glucose-derived carbon dot synthesis via Maillard reaction: from reaction mechanism to biomedical applications by Soohyun Cho, Hyemin Kim, Dongwook Song, Jungchan Jung, Sehyeon Park, Hyunda Jo, Sejeong Seo, Chaewon Han, Soye Park, Woosung Kwon, Hyunho Han

“…The optical properties and electronic band structures of G-CDs were analyzed using transient absorption and time-resolved photoluminescence spectroscopy. …”
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Ductular reaction or hepatic reparative complex: immunohistochemical features in liver cirrhosis in patients with chronic hepatitis by V. A. Tumanskiy, S. V. Fen’

“…The ductular reaction in liver cirrhosis in patients with chronic non-alcohol, alcoholic and viral hepatitis is the process of activation and evolution of a complex liver repair complex aimed at replenishing the deficit of hepatocytes and biliary structures. The reparative process, activating by liver cirrhosis on the background of the disturbed portal-lobular intercellular matrix and the progressive deficit of hepatocytes, does not provide the reconstruction of full-fledged liver structures, it is discordant towards the creation of new, functionally low-value hepatocellular pseudolobes and the accumulation of biliary terminals in fields of portal-septal fibrosis.…”
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The Emerging Role of Water Loss in Dog Aging by Gabriella Guelfi, Camilla Capaccia, Vicente Francisco Ratto, Antonello Bufalari, Leonardo Leonardi, Luca Mechelli, Simone Cenci, Margherita Maranesi

“…Aging involves progressive physiological changes, including the dysregulation of water homeostasis, essential for cellular function, neuronal signaling, and musculoskeletal integrity. …”
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Spatial phenotyping of human bronchial airways in obstructive lung disease by Latifa Khalfaoui, Raymond M. Moore, Jose. C. Villasboas, Kaitlyn R. Whitaker, Brenna C. Novotny, Michael A. Thompson, Christina M. Pabelick, Y. S. Prakash

“…Abstract Chronic respiratory diseases such as asthma and COPD involve interactions between multiple resident and immune cell types within bronchial airways, resulting in structural and functional changes. Thus cellular heterogeneity, arrangements and associated neighborhoods as well as interactions between cells and matrices represent intriguing yet challenging areas of study. …”
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Evolutionary systems biology of amino acid biosynthetic cost in yeast. by Michael D Barton, Daniela Delneri, Stephen G Oliver, Magnus Rattray, Casey M Bergman

“…Biosynthetic cost does, however, appear to affect rates of amino acid evolution in Saccharomyces, suggesting that expensive amino acids may only be used when they have specific structural or functional roles in protein sequences. …”
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Multispecies biofilm architecture determines bacterial exposure to phages. by James B Winans, Benjamin R Wucher, Carey D Nadell

“…The reasons underlying this variability are not well understood, and how phage-host encounters change within multispecies biofilms remains mostly unexplored at the cellular spatial scale. Here, we study how the cellular scale architecture of model 2-species biofilms impacts cell-cell and cell-phage interactions controlling larger scale population and community dynamics. …”
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Nanomolecular silencing of TSC22D4 mRNA via a DNAsome-siRNA for enhancing insulin sensitization in hepatocytes by Ameneh Mohammadi, Pedram Ebrahimnejad, Saeid Abediankenari, Zahra Kashi, Pooria Gill

“…Materials and Methods: The DNAsome was constructed using Y-DNA building blocks derived from three distinct DNA oligonucleotides. Its structural characteristics were analyzed through atomic force microscopy (AFM). …”
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Progress of estrogen receptor and spliceosome in endometrial carcinoma by Ziqi Hong, Ting Mo, Peiquan Zhou, Jian Chen, Xin Li

“…In addition, various post-translational modifications (PTMs) further affect ER-α cellular localization and ligand affinity, resulting in a change in the cellular function. …”
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Multiple regions of E6AP (UBE3A) contribute to interaction with papillomavirus E6 proteins and the activation of ubiquitin ligase activity. by Camille M Drews, Nicole Brimer, Scott B Vande Pol

“…How E6AP recognizes its cellular targets and how its ubiquitin ligase activity is triggered remain poorly understood, and HPV E6 proteins are models for these processes. …”
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Identifying Hub Genes and Pathways in Pancreatic Ductal Adenocarcinoma (PAAD): A comprehensive in silico study by Elham Karimi, Niloufar Sadat Kalaki, Seyed Mohammad Akrami

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