Cas12h is a crRNA-guided DNA nickase that can be utilized for precise gene editing by Wenwen Xiang, Xiaofeng Lin, Yunqian Yang, Linglong Huang, Ying Chen, Jiyun Chen, Liang Liu

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Conserved Molecular Mechanisms Underlying Homeostasis of the Golgi Complex by Cathal Wilson, Antonella Ragnini-Wilson

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Mast Cells, Cytokines and Asthma by Anthony E Redington, Peter H Howarth

“…This review details the current evidence that airway mast cells synthesize, store and release cytokines relevant to allergic inflammation and considers their potential involvement not only in the cellular influx within the airways but also in the fibrotic structural changes which are evident in chronic disease.…”
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In-silico and in-vitro morphometric analysis of intestinal organoids. by Sandra Montes-Olivas, Danny Legge, Abbie Lund, Alexander G Fletcher, Ann C Williams, Lucia Marucci, Martin Homer

“…Organoids offer a powerful model to study cellular self-organisation, the growth of specific tissue morphologies in-vitro, and to assess potential medical therapies. …”
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ECM-dependent regulation of septin 7 in focal adhesions promotes mechanosensing and functional response in fibroblasts by Wesley Sturgess, Swathi Packirisamy, Rodina Geneidy, Pontus Nordenfelt, Vinay Swaminathan

“…Using total internal reflection microscopy, we found that ECM-mediated integrin activation regulates spatially distinct Sept-7 structures in FAs. In perinuclear regions, ECM binding stabilized Sept-7 bundles at the back of FAs, while in the core of peripheral FAs high integrin activation promoted elongation of Sept-7 structures. …”
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Synergistically enhanced cryogenic strength and ductility in 304L stainless steel made by directed energy deposition additive manufacturing by Yen-Ting Chang, Andrew Hattoon Enriquez, Yuheng Nie, Didun Oladeji, Marie A. Charpagne

“…We leverage the rapid cooling rates and thermal cycling inherent to laser directed energy deposition (DED) additive manufacturing (AM), to synthesize 304L stainless steel with bimodal grain size distribution and intragranular cellular dislocation structures. We find that regardless of the printing strategy, DED AM 304L exhibits synergistically enhanced strength and ductility from room temperature down to cryogenic temperature (77 K); that surpasses the mechanical performance of 304L synthesized via conventional processes. …”
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X-ray micro-laminography of customized frequency acoustic panels by Lidija Korat bensa, Uroš Bohinc, Rok Rudolf, Rožle Repič

“…  Late progress in additive manufacturing has implemented the fabrication of customized porous and cellular structures at various levels. Structures with highly complex geometries and interconnected smaller pores or holes are impossible to produce by traditional fabrication methods, such as casting and extrusion. …”
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Biomimetic design and integrated biofabrication of an in-vitro three-dimensional multi-scale multilayer cortical model by Ling Wang, Luge Bai, Sen Wang, Jiajia Zhou, Yingjie Liu, Chenrui Zhang, Siqi Yao, Jiankang He, Chaozong Liu, Dichen Li

“…This study proposes a multi-scale design of a brain-like model with a biomimetic cortical structure, which includes the macroscopic structural features of six layers of different cellular components, as well as micrometer-scale continuous fiber structures running through all layers vertically. …”
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Improving experimental reproducibility through comprehensive research protocols by Ivana Novak

“…With this “In the Limelight: Research Protocols” special issue, FEBS Open Bio aims to highlight the critical importance of publishing reproducible and detailed scientific protocols that can be broadly adopted by laboratories working in molecular and cellular life sciences. This collection includes four protocols focused on sample preparation for structural analysis of macromolecules using X‐ray crystallography, covering both in vitro and in vivo approaches. …”
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Soluble αβ-tubulins reversibly sequester TTC5 to regulate tubulin mRNA decay by Alina Batiuk, Markus Höpfler, Ana C. Almeida, Deryn Teoh En-Jie, Oscar Vadas, Evangelia Vartholomaiou, Ramanujan S. Hegde, Zhewang Lin, Ivana Gasic

“…Here, we investigate how cells regulate TTC5 activity. Biochemical and structural proteomic approaches reveal that under normal conditions, soluble αβ-tubulins bind to and sequester TTC5, preventing it from engaging nascent tubulins at translating ribosomes. …”
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A nanobody that binds to the backside of the ubiquitin conjugating enzyme Ube2G2 differentially affects interactions with its partner E3 Ligases by Pavana Suresh, Charlotte Wijne, Zhen-Yu J. Sun, Nanette Becht, Ishani Sahay, Novalia Pishesha, Hidde Ploegh

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The Prevalence of STIV c92-Like Proteins in Acidic Thermal Environments by Jamie C. Snyder, Benjamin Bolduc, Mary M. Bateson, Mark J. Young

“…The STIV viral protein c92 is responsible for the formation of these lysis structures. We searched for c92-like proteins in viral sequences present in multiple viral and cellular metagenomic libraries from Yellowstone National Park acidic hot spring environments. …”
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Sequence signatures and mRNA concentration can explain two‐thirds of protein abundance variation in a human cell line by Christine Vogel, Raquel de Sousa Abreu, Daijin Ko, Shu‐Yun Le, Bruce A Shapiro, Suzanne C Burns, Devraj Sandhu, Daniel R Boutz, Edward M Marcotte, Luiz O Penalva

“…Abstract Transcription, mRNA decay, translation and protein degradation are essential processes during eukaryotic gene expression, but their relative global contributions to steady‐state protein concentrations in multi‐cellular eukaryotes are largely unknown. Using measurements of absolute protein and mRNA abundances in cellular lysate from the human Daoy medulloblastoma cell line, we quantitatively evaluate the impact of mRNA concentration and sequence features implicated in translation and protein degradation on protein expression. …”
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Photoswitchable phospholipids for the optical control of membrane processes, protein function, and drug delivery by Stefanie D. Pritzl, Johannes Morstein, Nikolaj A. Pritzl, Jan Lipfert, Theobald Lohmüller, Dirk H. Trauner

“…Abstract Recent insights into the function and composition of cell membranes have transformed our understanding from primarily viewing these structures as passive barriers to recognizing them as dynamic entities actively involved in many cellular functions. …”
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Out of balance: R-loops in human disease. by Matthias Groh, Natalia Gromak

“…R-loops are cellular structures composed of an RNA/DNA hybrid, which is formed when the RNA hybridises to a complementary DNA strand and a displaced single-stranded DNA. …”
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The autophagy protein ATG14 safeguards against unscheduled pyroptosis activation to enable embryo transport during early pregnancy by Pooja Popli, Arin K Oestreich, Vineet K Maurya, Marina N Rowen, Yong Zhang, Michael J Holtzman, Ramya Masand, John P Lydon, Shizuo Akira, Kelle Moley, Ramakrishna Kommagani

“…However, our understanding of the biological processes that preserve the oviductal cellular structure and functional integrity is limited. …”
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Microenvironments of tuberculous granuloma: advances and opportunities for therapy by Gesa Krueger, Gesa Krueger, Shah Faisal, Anca Dorhoi, Anca Dorhoi

“…These multicellular structures exhibit varying degrees of cellular complexity, are dynamic, and show considerable diversity within and between hosts. …”
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Serum-free endothelial cell culture medium for vascular smooth muscle cells sheet formation by Jing Yang, Xuheng Sun, Hongjing Jiang, Jiandong Li, Jierong Liang, Zhanyi Lin

“…Methods This study systematically compared cell sheets generated under SFM and 10% FBS culture conditions in terms of structure, cellular phenotype, and functional properties. …”
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Coiled-coil-domain-mediated TRIM E3 protein condensation modulates enzymatic activity and functional specificity by Qi Chen, Peiguo Yang

“…Summary: Tripartite motif (TRIM) proteins are E3 ligases with modular structures involved in diverse cellular functions. Whether biomolecular condensation broadly regulates TRIM proteins remains unclear. …”
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Coupled effects of morphology/length scale of the microstructure and microsegregation on the corrosion behaviour of a single-phase Zn-1wt%Cu alloy by Romulo S. Miranda, Talita Vida, Henrique S. Ligeiro, Noé Cheung, Fábio R. Simões, Amauri Garcia, Crystopher Brito

“…The results showed that coarser cellular structures demonstrated higher corrosion resistance, evidenced by greater charge transfer resistances and lower current densities. …”
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