Quantifying Intracellular Distributions of HaloTag-Labeled Proteins With SDS-PAGE and Epifluorescence Microscopy by Julia Shangguan, Ronald Rock

“…Counting protein molecules helps reveal the organization of components within cellular structures and the stoichiometries of protein complexes. …”
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Molecular Mechanisms of Alzheimer’s Disease Induced by Amyloid-β and Tau Phosphorylation Along with RhoA Activity: Perspective of RhoA/Rho-Associated Protein Kinase Inhibitors for... by Eun Hee Ahn, Jae-Bong Park

“…Neurons, with their complex processes and synapse connections, rely on cytoskeletal dynamics for structural support. Other brain cells, such as astrocytes, microglia, and oligodendrocytes, also depend on specific cytoskeletal structures to maintain their unique cellular architectures. …”
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GPR180 is a new member of the Golgi-dynamics domain seven-transmembrane helix protein family by Sarah-Ana Mitrovic, Chamalee Demalgiriya-Gamage, Lisa-Maria Winter, Tobias Kiechle, Rebecca Ebenhoch, Heike Neubauer, Birgit Stierstorfer, Lee Frego, Christian Wolfrum, Sophia Reindl, Herbert Nar

“…Using cellular imaging we show the localization of GPR180 in intracellular vesicular structures implying its exposure to acidic pH environments. …”
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OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images. by William F Dean, Tomasz J Nawara, Rose M Albert, Alexa L Mattheyses

“…Fluorescence Polarization Microscopy (FPM) is a powerful technique that goes beyond traditional imaging methods by allowing researchers to measure not only the localization of proteins within cells, but also their orientation or alignment within complexes or cellular structures. FPM can be easily integrated into standard widefield microscopes with the addition of a polarization modulator. …”
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Noninvasive method of monitoring the functional state of living cells by T. V. Parkhomenko, O. V. Galibin, V. V. Tomson

“…Usage of membrane PSP is of great importance for assessing both the viability and functional integrity of the cells and their structural components (mitochondria, nuclei, cytoplasmic membranes, ion channels). …”
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Externally Triggered Activation of Nanostructure-Masked Cell-Penetrating Peptides by Gayong Shim

“…Transmission electron microscopy revealed that the synthesized DNA nanoflower structures were approximately 150–250 nm in size. Structural and functional analyses confirmed that the system remained inert under physiological conditions and was rapidly activated by plasma treatment. …”
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An open-source tool for analysis and automatic identification of dendritic spines using machine learning. by Michael S Smirnov, Tavita R Garrett, Ryohei Yasuda

“…Synaptic plasticity, the cellular basis for learning and memory, is mediated by a complex biochemical network of signaling proteins. …”
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Bistability and oscillations in the Huang-Ferrell model of MAPK signaling. by Liang Qiao, Robert B Nachbar, Ioannis G Kevrekidis, Stanislav Y Shvartsman

“…Physicochemical models of signaling pathways are characterized by high levels of structural and parametric uncertainty, reflecting both incomplete knowledge about signal transduction and the intrinsic variability of cellular processes. …”
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The Chikungunya Virus nsP3 Macro Domain Inhibits Activation of the NF-κB Pathway by Grace C. Roberts, Nicola J. Stonehouse, Mark Harris

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Proteomic Characterization of Extracellular Vesicles from Human Neural Precursor Cells: A Promising Advanced Therapy for Neurodegenerative Diseases by Stricker PEF, de Oliveira NB, Mogharbel BF, Irioda AC, da Rosa NN, Lührs L, Saçaki CS, Munhoz da Rocha I, Alves LR, Poubel SB, Cardoso da Silva J, Carvalho PC, Fischer JSG, de Carvalho KAT

“…NTA and TEM of the EVs exhibited characteristic size, shape, and structural integrity consistent with the criteria established by the International Society for Extracellular Vesicles (ISEV). …”
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Oxidative Stress: A Possible Trigger for Pelvic Organ Prolapse by Radu Dragos Marcu, Dan Liviu Dorel Mischianu, Lucian Iorga, Camelia Cristina Diaconu, Mihaela Surcel, Adriana Narcisa Munteanu, Carolina Constantin, Gheorghita Isvoranu, Ovidiu Gabriel Bratu

“…The purpose of this paper is to underline the importance of oxidative stress in the pathogenesis of pelvic organ prolapse, as well as to highlight the main oxidative stress molecular changes that appear at the level of the pelvic-supportive structures. Sustained mechanical stress is proven to be a key factor in the appearance of pelvic organ prolapse, correlating with increased levels of free radicals production and mitochondrial-induced fibroblasts apoptosis, the rate of cellular apoptosis depending on the intensity of the mechanical stress, and the period of time the mechanical stress is applied. …”
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Overexpression of the microtubule-binding protein CLIP-170 induces a +TIP network superstructure consistent with a biomolecular condensate. by Yueh-Fu O Wu, Annamarie T Bryant, Nora T Nelson, Alexander G Madey, Gail F Fernandes, Holly V Goodson

“…Proper regulation of microtubule (MT) dynamics is critical for cellular processes including cell division and intracellular transport. …”
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Berry Fruit Extracts as Topical Cosmeceuticals for Skin Health Applications: A Systematic Review by Filipe Silveira Azevedo, Allan Rodrigues Pires, Mary Ann Lila, Giuseppe Valacchi, Roberta Targino Hoskin, Mariaurea Matias Sarandy, Rômulo Dias Novaes, Reggiani Vilela Goncalves

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Vaccinia virus proteins A52 and B14 Share a Bcl-2-like fold but have evolved to inhibit NF-kappaB rather than apoptosis. by Stephen C Graham, Mohammad W Bahar, Samantha Cooray, Ron A-J Chen, Daniel M Whalen, Nicola G A Abrescia, David Alderton, Raymond J Owens, David I Stuart, Geoffrey L Smith, Jonathan M Grimes

“…Structure-based phylogenetic analysis of 32 cellular and viral Bcl-2-like protein structures reveals that A52 and B14 are more closely related to each other and to VACV N1 and myxoma virus M11 than they are to other viral or cellular Bcl-2-like proteins. …”
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The Smc5-Smc6 complex is required to remove chromosome junctions in meiosis. by Sarah Farmer, Pedro A San-Segundo, Luís Aragón

“…Meiosis, a specialized cell division with a single cycle of DNA replication round and two consecutive rounds of nuclear segregation, allows for the exchange of genetic material between parental chromosomes and the formation of haploid gametes. The structural maintenance of chromosome (SMC) proteins aid manipulation of chromosome structures inside cells. …”
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The involvement of oxidative stress in non-Hodgkin’s lymphomas; a review of the literature by Ramona Ingrid Corbeanu, Amelia Maria Găman

“…Oxidative stress exerts noxious effects on the cell structures by inducing structural changes in membranes, lipids, proteins or DNA. …”
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Examining Form and Function of Dendritic Spines by Kevin F. H. Lee, Cary Soares, Jean-Claude Béïque

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Atomic-level investigation of KCNJ2 mutations associated with ventricular arrhythmic syndrome phenotypes by Saba Munawar, Corey L. Anderson, Louise Reilly, Ryan Woltz, Yusra Sajid Kiani, Nipavan Chiamvimonvat, Lee L. Eckhardt

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Comprehensive profiling of the catalytic conformations of human Guanylate kinase by Lei Wang, Zihuan Li, Yumi Xuan, Jingkun Qin, Shuju Li, Fumei Zhong, Yuexiao Song, Kanglong Yang, Mengqi Lv, Fudong Li, Zhang Jiahai, Yueyin Pan, Shouhong Guang, Yuzheng Zhao, Yunyu Shi, Xing Liu, Yingying Du, Jia Gao, Ke Ruan

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Ion Channel Regulation in Caveolae and Its Pathological Implications by Jianyi Huo, Liangzhu Mo, Xiaojing Lv, Yun Du, Huaqian Yang

“…Caveolae are distinctive, flask-shaped structures within the cell membrane that play critical roles in cellular signal transduction, ion homeostasis, and mechanosensation. …”
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