Correlative Imaging and super resolution microscopy studies reveal complexities in determining live-dead state of bacteria by Jiaqi Luo, Rasmita Raval

“…This study investigates the live-dead state of cells in early-stage Staphylococcus aureus biofilms using correlative Fluorescence Microscopy (FM), Scanning Electron Microscopy (SEM) and super resolution Structural Illumination Microscopy (SIM). Correlative imaging data obtained at the single-cell level show that the physiological states of individual bacterial cells indicated by the SYTO 9 – propidium iodine counterstain in FM does not correlate directly with the detailed cell morphology observed by SEM. …”
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scFv intrabody targeting wildtype TDP-43 presents protective effects in a cellular model of TDP-43 proteinopathy. by Yara Al Ojaimi, Rudolf Hergesheimer, Anna A Chami, Hugo Alarcan, Johanna Augros, Audrey Dangoumau, Shanez Haouari, Jérôme Bourgeais, Antoine Lefevre, Samira Osman, Patrick Emond, Patrick Vourc'h, Christian R Andres, Philippe Corcia, Olivier Herault, Pierre Martineau, Débora Lanznaster, Hélène Blasco

“…We generated a 3D structure of full length wt TDP-43 in silico, and used it for epitope mapping. …”
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Sub-cellular level resolution of common genetic variation in the photoreceptor layer identifies continuum between rare disease and common variation. by Hannah Currant, Tomas W Fitzgerald, Praveen J Patel, Anthony P Khawaja, UK Biobank Eye and Vision Consortium, Andrew R Webster, Omar A Mahroo, Ewan Birney

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scGraphformer: unveiling cellular heterogeneity and interactions in scRNA-seq data using a scalable graph transformer network by Xingyu Fan, Jiacheng Liu, Yaodong Yang, Chunbin Gu, Yuqiang Han, Bian Wu, Yirong Jiang, Guangyong Chen, Pheng-Ann Heng

“…Through an iterative refinement process, scGraphformer constructs a dense graph structure that captures the full spectrum of cellular interactions. …”
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Development of pea protein-derived 3D foam scaffolds cross-linked with heat and tannic acid for cellular agriculture applications by Woo-Ju Kim, Yoonbin Kim, Begum Koysuren, Nitin Nitin

“…Plant protein-based, 3D-structured scaffolds that mimic the mechanical properties of conventional meat products while supporting cytocompatibility and cellular growth are essential for developing alternative meat products. …”
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How the chromatin landscape influences nuclear morphology by Sourabh Sengupta, Haritha Prabha, Daniel L. Levy

“…In this review we discuss how the chromatin landscape regulates nuclear morphology in both normal and diseased cellular states. Additionally, we highlight emerging technologies that promise to bridge critical gaps in our understanding of nuclear morphology, including new approaches to probe nuclear structure and the use of synthetic cells.…”
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Plug-and-display nanoparticle immunization of the core epitope domain induces potent neutralizing antibody and cellular immune responses against PEDV by Minghui Li, Yilan Chen, Siqiao Wang, Xueke Sun, Yongkun Du, Siyuan Liu, Ruiqi Li, Zejie Chang, Peiyang Ding, Gaiping Zhang

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Layered Growth of 3D Snowflake Subject to Membrane Effect and More than One Nucleation Center by Means of Cellular Automata by César Renán Acosta, Irma Martín, Gabriela Rivadeneyra

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New molecular mechanisms to explain the neuroprotective effects of insulin-like growth factor II in a cellular model of Parkinson’s disease by Silvana-Yanina Romero-Zerbo, Nadia Valverde, Silvia Claros, Pablo Zamorano-Gonzalez, Federica Boraldi, Francesco-Demetrio Lofaro, Estrella Lara, Jose Pavia, Maria Garcia-Fernandez, Belen Gago, Elisa Martin-Montañez

“…Some of these proteins were found to be involved in mitochondrial structure and function, and cell cycle regulation. …”
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Control of the Properties of the Voronoi Tessellation Technique and Biomimetic Patterns: A Review by Ana Karilú Arvizu Alonso, Eddie Nahúm Armendáriz Mireles, Carlos Adrián Calles Arriaga, Enrique Rocha Rangel

“…The cellular behavior of Voronoi tessellation has generated interest due to its applicability in various fields and its notable structural properties. …”
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Analysis of genetic diversity of Zymoseptoria tritici populations in central and south-eastern Ethiopia by Ayantu Tucho, Ayantu Tucho, Tilahun Mekonnen, Farideh Ghadamgahi, Samrat Ghosh, Diriba Muleta, Kassahun Tesfaye, Kassahun Tesfaye, Eu Shang Wang, Tesfaye Alemu, Ramesh Raju Vetukuri

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Effect of Boundary Conditions and Angles on the Compressive Performance of the Corner Element by Bing Xu, Lang Wang, Qin Liu, Rui Wang, Bo Xu, Bing Kong, Dan Zhang

“…The corner element is the most basic constitutive element of multi-cellular thin-walled structures; however, scenarios for using relevant theories are limited. …”
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Wood Particles of Various Sizes from Primary Wood Processing as Filler in Rigid Polyurethane Foams by Joanna Walkiewicz, Dorota Dukarska, Radosław Mirski

“…In each case, a decrease in brittleness was observed, which could be attributed to changes in cellular structure. Despite the fact, that some decrease was observed, the obtained results are satisfactory.…”
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Advances in Molecular Function and Recombinant Expression of Human Collagen by Wenli Sun, Mohamad Hesam Shahrajabian, Kun Ma, Shubin Wang

“…These proteins play critical roles in maintaining mechanical characteristics, tissue organization, and structural integrity. Collagens regulate cellular processes such as proliferation, migration, and differentiation through interactions with cell surface receptors. …”
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Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET by Yanyan Xue, Yi Sun, Yichun Xia, Xiuming Liu, Hua Dai

“…Riboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg²⁺ and their ligands playing pivotal roles in this process. The dynamic structural mechanism by which the S-adenosyl-L-methionine (SAM) responsive SAM-VI riboswitch (riboSAM) regulates the downstream SAM synthase gene translation remains unclear. …”
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Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (<i>Stagonosporopsis cucurbitacearum</i>) of Melon Plant Introduction (PI) 420145 by Rui Zhang, Jing Chen, Yongbing Zhang, Xiumei Lu, Chunyan Cheng, Ji Li, Qunfeng Lou, Yuhui Wang, Chuntao Qian

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Molecular Determinants of Protein Pathogenicity at the Single‐Aggregate Level by Agnieszka Urbanek, Emma F. Garland, Emily E. Prescott, Marianne C. King, Anna Olerinyova, Hollie E. Wareing, Nia Georgieva, Ellie L. Bradshaw, Svetomir B. Tzokov, Alexander Knight, Alexander I. Tartakovskii, Tarja Malm, J Robin Highley, Suman De

“…Abstract Determining the structure‐function relationships of protein aggregates is a fundamental challenge in biology. …”
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Expose flexible conformations for intrinsically disordered protein by Jiaan Yang, Wenxin Ji, Wen Xiang Cheng, Gang Wu, Si Tong Sheng, Peng Zhang, Jun Lin, Xiaojia Chen, Qiong Shi

“…Three proteins, human cellular tumor antigen P53, human alpha-synuclein, and human protamine-2, are taken as samples for demonstration of how to obtain their folding conformation structures for intrinsically disordered proteins. …”
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Extensive folding variability between homologous chromosomes in mammalian cells by Ibai Irastorza-Azcarate, Alexander Kukalev, Rieke Kempfer, Christoph J Thieme, Guido Mastrobuoni, Julia Markowski, Gesa Loof, Thomas M Sparks, Emily Brookes, Kedar Nath Natarajan, Stephan Sauer, Amanda G Fisher, Mario Nicodemi, Bing Ren, Roland F Schwarz, Stefan Kempa, Ana Pombo

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Integration of T cell repertoire, CyTOF, genotyping and symptomatology data reveals subphenotypic variability in COVID-19 patients by Fernando Marín-Benesiu, Lucia Chica-Redecillas, Sergio Cuenca-López, Carmen Entrala-Bernal, Sara Martín-Esteban, Maria Jesús Alvarez-Cubero, Luis Javier Martínez-González

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