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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression
Published 2019-01-01“…The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). …”
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METTL3-Mediated m6A Modification Regulates the Osteogenic Differentiation through LncRNA CUTALP in Periodontal Mesenchymal Stem Cells of Periodontitis Patients
Published 2024-01-01“…We carried out a series of experiments, including methylated RNA immunoprecipitation-PCR, quantitative real-time polymerase chain reaction, and western blotting. The expressions of alkaline phosphatase (ALP), Runx2, Col1, Runx2 protein level, ALP staining, and Alizarin red staining were used to demonstrate the degree of osteogenic differentiation. …”
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Prevalence, risk factors, and prognostic implications of intraoperative bleeding during CF-LVAD implant
Published 2025-02-01Get full text
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Prevalence, incidence and determinants of QuantiFERONTM positivity in South African schoolchildren
Published 2024-05-01Get full text
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Primary results from the Japanese Heart Failure and Sudden Cardiac Death Prevention Trial (HINODE)
Published 2022-06-01Get full text
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Relieving Sore Throat Formula Exerts a Therapeutic Effect on Pharyngitis through Immunoregulation and NF-κB Pathway
Published 2020-01-01“…Alteration of NF-κB pathway was determined by Western blotting, immunofluorescence, and PCR. The in vivo experiments demonstrated that the RSTF could significantly relieve the symptoms of pharyngitis. …”
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Remote Sensing of Sun-Induced Fluorescence in a Deep Lake: Disentangling Quenching Mechanisms Improves Relationship With Chlorophyll-a Concentration Estimates
Published 2025-01-01“…We assembled a unique dataset collected between 2018 and 2022 by an autonomous profiler in Lake Geneva (Western Europe). We used NPQ-influenced chl-a estimates from the fluorometer and NPQ-corrected chl-a estimates to distinguish between PQ and NPQ cases. …”
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FAM134B-mediated endoplasmic reticulum autophagy protects against cisplatin-induced spiral ganglion neuron damage
Published 2025-01-01“…The expression of FAM134B, proteins associated with ER stress, autophagy and apoptosis was measured by western blot. The reactive oxygen specie (ROS) levels were evaluated by MitoSOX Red and 2′,7′-Dchlorodihydrofluorescein diacetate (DCFH-DA) probe. …”
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Deep Searches for Radio Pulsations and Bursts from Four Magnetar and a Magnetar-like Pulsar with FAST
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Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease
Published 2025-01-01“…Preliminary validation through in vitro and in vivo experiments, including observation of changes in mitochondrial morphology and dynamics using probes such as Mitotracker Red, and evaluation of the effect of losartan on key events of mitochondrial homeostasis perturbation using electron microscopy, laser confocal microscopy, immunofluorescence, and Western blotting. …”
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Cancer cells avoid ferroptosis induced by immune cells via fatty acid binding proteins
Published 2025-02-01“…Methods included mass spectrometry-based lipidomics, targeted lipidomics, Oil Red O staining, Seahorse analysis, quantitative PCR, immunohistochemistry, PPARγ transcription factor assays, ChIP-seq, untargeted lipidomic analysis, ROS assay, ex vivo co-culture of CD8+ T cells with cancer cells, ATAC-seq, RNA-seq, Western blotting, co-immunoprecipitation assay, flow cytometry and Imaging Mass Cytometry. …”
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