Optimized Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) Production by Moderately Haloalkaliphilic Bacterium Halomonas alkalicola Ext by Martin N. Muigano, Sylvester E. Anami, Justus M. Onguso, Godfrey O. Mauti

“…Sudan Black B and Nile Red A staining showed that bacterium had distinct ability for accumulation of PHAs. …”
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B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts by Qiao Kang, Wenhao Wang, Shengtao Wu, Ganggang Hu

“…Western blotting for protein expression detection together with real-time quantitative polymerase chain reaction for mRNA expression measurement were carried out on those related to hBMSC proliferation and differentiation. …”
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Investigation of the osteogenic effects of ICA and ICSII on rat bone marrow mesenchymal stem cells by Zhangshun Yao, Weixiang Huang, Yan Yang, Leiyan Zou, Yunpeng Zhang, Jing Zhang, Guangming Luo

“…Subsequently, we compared the vascularization and osteogenic capacity of the two groups through alizarin red staining assay, Western Blot, and RT-PCR. Subsequently, we assessed the phosphorylated and non-phosphorylated expression of JNK, ERK1/2, P38, and AKT at different time intervals. …”
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Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia by Yanhua Qin, Weiming Hu, Yang Yang, Zhiying Hu, Weiyun Li, Marong Fang

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Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways by Xiyao Pang, Ying Zhuang, Zehan Li, Shuanglin Jing, Qin Cai, Fengge Zhang, Changao Xue, Jinhua Yu

“…Alkaline phosphatase (ALP) activity, alizarin red staining, Western blot, and RT-PCR were carried out to detect the odonto/osteogenic differentiation of PTH-treated SCAPs as well as the participation of the MAPK signaling pathway. …”
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Treatment of Radiation Bone Injury with Transplanted hUCB-MSCs via Wnt/β-Catenin by Yufeng Zhang, Huaxin Deng, Zhiqiang Yang, Zhe Chen, Sheng Zhang, Yufan Zhu, Min Yang, Houcheng Zhong, Fuling Zhou, Yuanlong Xie, Lin Cai

“…The differentiation of osteoblasts was measured by ALP and Alizarin red staining and transmission electron microscopy. …”
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Enamel Matrix Derivative Enhances the Odontoblastic Differentiation of Dental Pulp Stem Cells via Activating MAPK Signaling Pathways by Beidi Zhang, Min Xiao, Xiaogang Cheng, Yu Bai, Hanze Chen, Qing Yu, Lihong Qiu

“…In vitro phase: cell proliferation was assessed by a cell counting kit-8 (CCK-8) assay; then, alkaline phosphatase (ALP) activity and staining, alizarin red staining, real-time RT-PCR, and western blot analysis were conducted to determine the odontoblastic potential and involvement of MAPK signaling pathways. …”
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Bisphenol S Induces Lipid Metabolism Disorders in HepG2 and SK-Hep-1 Cells via Oxidative Stress by Kai-Xing Lin, Zi-Yao Wu, Mei-Lin Qin, Huai-Cai Zeng

“…Increased lipid droplets were observed using morphological Oil Red O staining. Our study demonstrates that BPS may cause lipid accumulation by increasing oxidative stress and perturbing cellular lipid metabolism.…”
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RETRACTED ARTICLE: Emodin enhances osteogenesis and inhibits adipogenesis by Feng Yang, Pu-wei Yuan, Yang-Quan Hao, Zheng-Mao Lu

“…In vitro, we analyze the effect of emodin—at concentrations between 0.1 μM and 10 μM-on the processes of inducing osteogenesis and inhibiting adipogenesis in BMSCs by ALP, Oil red O staining, real time RT-PCR and western blot. …”
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Cebpb Regulates Skeletal Stem Cell Osteogenic Differentiation and Fracture Healing via the WNT/β-Catenin Pathway by Jiansong Wang, Chensong Yang, Fanyu Kong, Zhi Zhang, Shengchao Ji, Guixin Sun

“…The expression of Cebpb, osteogenesis-related genes (Runx2, Sp7, and Bglap2), and markers for Notch, Hedgehog, MAPK, BMP2/SMAD, and WNT/β-catenin signaling pathways (Hes1, Gli1, p-Erk1/2, p-Smad1/5/9, and β-catenin) were detected in SSCs with qPCR or western blot, respectively. Alkaline phosphatase assay and alizarin red S staining were used to illustrate the osteogenic differentiation ability of SSCs in vitro. …”
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Sorghum yield prediction based on remote sensing and machine learning in conflict affected South Sudan by John Karongo, Joseph Ivivi Mwaniki, John Ndiritu, Victor Mokaya

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Dias-Logan syndrome with a p.Leu360Profs*212 heterozygous pathogenic variant of in a Chinese patient: A case report by Yizhuo Shu, Xiaoling Chen, Zhuoqun Wei, Chunyue Chen

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Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice by Liang Dong, Lang Li

“…Pulmonary fibrosis was evaluated by Picrosirius red staining, immunohistochemistry for α-SMA and TGF-β1, and hydroxyproline assay and RT-qPCR for Col1α1 and Col3α1. …”
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Inhibition of miR-652-3p Regulates Lipid Metabolism and Inflammatory Cytokine Secretion of Macrophages to Alleviate Atherosclerosis by Improving TP53 Expression by Haiyun Liu, Changpeng Zuo, Lijuan Cao, Naiquan Yang, Tingbo Jiang

“…The protein levels of ABCA1, ABCG1, PPARα, CRT1, ADRP, and ALBP were detected by western blot assay. Based on the TargetScan database, the TP53 3′UTR region had complementary bases with miR-652-3p, which was also verified by dual-luciferase reporter gene assay. …”
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Lysophosphatidic Acid Induced Apoptosis, DNA Damage, and Oxidative Stress in Spinal Cord Neurons by Upregulating LPA4/LPA6 Receptors by Yifan Yang, Jing Xu, Qingxin Su, Yiran Wu, Qizheng Li, Zongren Ma, Tao Ding

“…After clinical detection of LPA secretion, spinal cord neurons were treated with LPA (18 : 1); cell viability was analyzed by MTT assay, and LDH leakage was detected by LDH kit; cell apoptosis was detected by flow cytometry; ROS production was measured by DCFDA staining and MitoSOX Red Staining; the activation of the Gα12/Gα13 signaling pathway was detected by serum response factor response element (SRF-RE) luciferase reporter gene; the relationship among LPA, LPA4/6, and ROCK was examined by western blotting. …”
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Morphological and phylogenetic features of the Crimean population of <i>Juniperus deltoids</i> R.P. Adams by A. O. Lantushenko, O. O. Korenkova, A. A. Syrovets, Ya. V. Meger, P. A. Korenkov, O. M. Shevchuk

“…A similar phenomenon is characteristic of the Western Mediterranean populations of J. oxycedrus. …”
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LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation by Litao Liang, Wenbo Jia, Jinyi Wang, Yanzhi Feng, Deming Zhu, Wenhu Zhao, Chao Xu, Xiangyu Ling, Qingpeng Lv, Xiaoming Ai, Lianbao Kong, Wenzhou Ding

“…Lipid metabolism plays a crucial role in the metabolic reprogramming observed in HCC, although the underlying mechanisms are still being elucidated. Nile red staining and lipid assays showed that LRP11 knockdown significantly reduces lipid accumulation in HCC cells, with a concurrent decrease in key lipid metabolism markers such as FSAN, ACLY and ACSL4, as demonstrated by Western blotting. …”
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A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue by Debora Lo Furno, Adriana Carol Eleonora Graziano, Rosanna Avola, Rosario Giuffrida, Vincenzo Perciavalle, Francesco Bonina, Giuliana Mannino, Venera Cardile

“…To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed. …”
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PPARγ Antagonizes Hypoxia-Induced Activation of Hepatic Stellate Cell through Cross Mediating PI3K/AKT and cGMP/PKG Signaling by Qinghui Zhang, Shihao Xiang, Qingqian Liu, Tao Gu, Yongliang Yao, Xiaojie Lu

“…Liver sections were stained with HE and Sirius red staining 8 weeks later. HSCs were exposed to hypoxic environment in the presence or absence of rosiglitazone, and expression of PPARγ and two fibrosis markers, α-SMA and desmin, were measured using western blot and immunofluorescence staining. …”
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Spectroscopic (FT-IR and FT-Raman) and quantum chemical study on monomer and dimer of benznidazole from DFT and molecular docking approaches by Tirth Raj Paneru, Manoj Kumar Chaudhary, Poonam Tandon, Bhawani Datt Joshi, Beatriz Pinheiro Bezerra, Alejandro Pedro Ayala

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