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Lupeol Attenuates Palmitate-Induced Hypertrophy in 3T3-L1 Adipocytes
Published 2025-01-01“…Lipid droplet size was assessed and validated by Oil Red O staining. Western blot analysis was performed to measure the expression of adipogenic and inflammatory markers. …”
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MEK1/2 Inhibitor (GDC0623) Promotes Osteogenic Differentiation of Primary Osteoblasts Inhibited by IL-1β through the MEK-Erk1/2 and Jak/Stat3 Pathways
Published 2021-01-01“…Cell proliferation levels were evaluated by the cell counting kit 8 (CCK8), EdU assay, and western blotting [proliferating cell nuclear antigen (PCNA) and Cyclin D1]. …”
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Deoxycholate, an Endogenous Cytotoxin/Genotoxin, Induces the Autophagic Stress-Survival Pathway: Implications for Colon Carcinogenesis
Published 2009-01-01“…The DOC-induced increase in autophagy was documented by an increase in autophagic vacuoles (detected using transmission electron microscopy, increased levels of LC3-I and LC3-II (western blotting), an increase in acidic vesicles (fluorescence spectroscopy of monodansycadaverine and lysotracker red probes), and increased expression of the autophagic protein, beclin-1 (immunohistochemistry/western blotting). …”
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Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacol...
Published 2023-01-01“…Transmission electron microscopy, immunofluorescence, and Western blot analyses were used to evaluate autophagy activity. …”
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DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway
Published 2025-01-01“…Commercially available triglyceride and cholesterol detection kits, as well as Nile Red and Oil red O staining were used to measure lipid metabolism. …”
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L’anticommunisme, l’autre moteur de Jean-Marie Le Pen
Published 2024-12-01“…Without claiming to be exhaustive, here is a look back at 75 years of hate for the red.…”
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Avian Influenza a (H5N1) Infection with Respiratory Failure and Meningoencephalitis in a Canadian Traveller
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The People's Commissariat of Foreign Affairs (NKID) of the USSR in the City of Kuibyshev (1941-1943)
Published 2020-09-01“…Due to the opposition of the Western powers, domestic diplomacy failed to create a collective security system to prevent the aggression of Germany, Italy and Japan. …”
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L’insaisissable religion des Taïnos. Esquisse d’anthropologie historique
Published 2003-06-01“…The elusive religion of the Taïnos: a historical anthropological outline. Having disappeared shortly after the discovery of the New World, the Tainos today are back in vogue, especially where the zemis are concerned, mysterious multiform objects that had already struck the attention of the earliest Western observers. …”
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Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α
Published 2018-01-01“…After stimulating PDLSCs with TNF-α and AZM, we analyzed alkaline phosphatase staining, alkaline phosphatase activity, and alizarin red staining to detect osteogenic differentiation. …”
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High hemolytic activity in Staphylococcus aureus t1081/ST45 due to increased hla protein production and potential RNAIII-independent regulation
Published 2025-02-01“…The expression of the transcriptional regulators mgrA and saeR was also similar between the two groups. Western blotting revealed increased Hla protein in the t1081 isolates. …”
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miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3
Published 2021-01-01“…The ability of BMSC differentiation to osteoblast was tested by alkaline phosphatase (ALP) and alizarin red staining (ARS). The expression of osteogenic genes (Runx2, Bmp2, and OCN) was examined via quantitative RT-PCR and western blot. …”
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Pesticides Are Involved With Population Declines of Amphibians in the California Sierra Nevadas
Published 2001-01-01“…These species include the threatened red-legged frog (Rana aurora), foothill yellow-legged frog (R. boylii), mountain yellow-legged frog (R. muscosa), Cascades frog (Rana cascadae), western toad (Bufo boreas) and Yosemite toad (B. canorus). …”
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Shengxue Busui Decoction activates the PI3K/Akt and VEGF pathways, enhancing vascular function and inhibiting osteocyte apoptosis to combat steroid-induced femoral head necrosis
Published 2025-01-01“…In vitro, Dex-induced osteoblast apoptosis in MC3T3-E1 cells was examined to assess SBD’s effect on osteoblast proliferation, apoptosis, and signaling. Western blotting analyzed Caspase-9, Caspase-3, Bax, Bcl-2, and pathway-related proteins. …”
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Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis
Published 2025-01-01“…We employ a combination of Western blotting and mass spectrometry to analyze proteins in var2 and abc1k1. …”
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Preparation of the New Military Units for the Front (the Experience of the Siberian Military District)
Published 2015-06-01“…Incompleteness of reforms and a number of other reasons, led from the first days of the war to serious losses in the ranks of the Red Army. To make up for the losses it was required to mobilize significant resources, a substantial part of which has been lost in the abandonment of the western Soviet territories. …”
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Stimulation of Alpha1-Adrenergic Receptor Ameliorates Cellular Functions of Multiorgans beyond Vasomotion through PPARδ
Published 2020-01-01“…The expression of various biomarkers involved in ATP production was estimated through Western blotting, reverse transcription polymerase chain reaction, oxygen consumption rate, enzyme-linked immunosorbent assay (ELISA), fluorescence staining, and Oil red O staining in several cell lines (skeletal muscle, cardiac muscle, liver, macrophage, vascular endothelial, and adipose cells). …”
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Bone marrow mesenchymal stem cell-derived extracellular vesicles alleviate diabetes-exacerbated atherosclerosis via AMPK/mTOR pathway-mediated autophagy-related macrophage polariza...
Published 2025-01-01“…RAW264.7 cells were transduced with autophagy marker mRFP-GFP-LC3 lentivirus and analyzed with IF and western blotting. Results Diabetic mice (DA group) had larger aortic plaque areas and lower collagen content than the HFD mice. …”
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