Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG by Martina Bracco, Tuomas P. Mutanen, Domenica Veniero, Gregor Thut, Edwin M. Robertson

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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

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Protocol for live imaging of axonal transport in iPSC-derived iNeurons by Dan Dou, Erika L.F. Holzbaur, C. Alexander Boecker

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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

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Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

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Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time by Amy Llewellyn, Rachel Barrow-McGee, Julia Stevenson, Jasmine Gore, Kalnisha Naidoo

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4D light sheet imaging, computational reconstruction, and cell tracking in mouse embryos by Martin H. Dominguez, Jonathon M. Muncie-Vasic, Benoit G. Bruneau

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Protocol for the quantitative detection of mtDNA in the supernatants of activated human naive CD8+ T cells by Linlin Li, Jun Jin

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Protocol to detect neutral lipids with BODIPY staining in myeloid-derived suppressor cells in mouse mammary tumors by Ukjin Kim, Rumela Chakrabarti

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Protocol for in vivo chromatin immunoprecipitation on purified chromatin isolated from mouse liver nuclei by Lei Li, May G. Akl, Scott B. Widenmaier

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Protocol for generating a pancreatic cancer organoid associated with heterogeneous tumor microenvironment by Kenta Takeuchi, Shunsuke Tabe, Yuya Yamamoto, Kenta Takahashi, Megumi Matsuo, Yasuharu Ueno, Masayuki Ohtsuka, Soichiro Morinaga, Yohei Miyagi, Tomoyuki Yamaguchi, Naoki Tanimizu, Hideki Taniguchi

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Protocol for the three-dimensional analysis of rodent skeletal muscle by Smrithi Karthikeyan, Yoko Asakura, Mayank Verma, Atsushi Asakura

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Protocol to boost the robustness and accuracy of spatial transcriptomics algorithms using ensemble techniques by Jiazhang Cai, Shushan Wu, Huimin Cheng, Wenxuan Zhong, Guo-Cheng Yuan, Ping Ma

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Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

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Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq by Shintaro Funasaki, Yuri Miyamura, Shunsuke Kamei, Akhinur Rahman, Masaya Yamazaki, Shingo Usuki, Keiichiro Yasunaga, Yorifumi Satou, Hiroto Ohguchi, Takashi Minami

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Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells by Sherry Li Zheng, Jonas L. Fowler, Julie Y. Chen, Christopher Li, Elaine Lin, Alana T. Nguyen, Angela Chen, George Q. Daley, Lay Teng Ang, Kyle M. Loh

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Protocol for recombinant rabies virus titration by quantitative PCR by He Zhang, Xueping Gao, Xiangyu Ge, Xiao Wang, Minghui Song, Xia Zhang, Lei Jin

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A 2D cell segmentation protocol for monitoring multiple STAT signaling pathways by fluorescence microscopy by Gergő Szanda, Éva Wisniewski, László Barna, Gábor Turu, Ken Mackie

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Protocol for multiplexed RNAscope-based imaging of mRNAs in whole-mount adult Drosophila brains by Meilin Wu, Vanessa Lambatan, Peng Guo, William J. Joiner

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