CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…However, a suitable antibody for HNF1A is currently unavailable. The present study is aimed at establishing subtyping in PDAC organoids using an alternative marker. …”
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Emergency medicine pharmacists’ interventions in the tertiary hospitals’ emergency departments in Malaysia by Hock Peng Koh, Haliza Ab Jalal, Yee Ping Koo, Mary Siew Yng Chen, Manimegahlai Selvaratanam, Anaanthan Bhuvanendran Pillai, Wai Kit Wong, Xin Nee Guee, Sherene Su Ann Tan, Shirlyn Tan, Lih Jiuan Teh, Rosalind Guen Lin Sia

“…In Malaysia, data on interventions done by EM pharmacists in the EDs was unavailable. This study aimed to assess the type of interventions done by EM pharmacists in the ED of tertiary public hospitals in Malaysia.Methods: This cross-sectional, multicenter study involved EM pharmacists from 14 tertiary hospitals in Malaysia. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…Gene editing has not been established in LMB due to the unavailability of one-cell fertilized eggs. In this study, three types of pigment cells, melanophores, iridophores and xanthophores were identified in LMB and melanophores were found first appeared in the iris of eyes in larvae 1.5 days after hatching. …”
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A Network Pharmacology-Based Strategy for Unveiling the Mechanisms of Tripterygium Wilfordii Hook F against Diabetic Kidney Disease by Yuyang Wang, Tongtong Liu, Fang Ma, Xiaoguang Lu, Huimin Mao, Weie Zhou, Liping Yang, Ping Li, Yongli Zhan

“…However, a holistic network pharmacology strategy to investigate the active components and therapeutic mechanism underlying DKD is still unavailable. Methods. We collected TwHF ingredients and their targets by traditional Chinese Medicine databases (TCMSP). …”
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Analysis of mutations in CDC27, CTBP2, HYDIN and KMT5A genes in carotid paragangliomas by E. N. Lukyanova, A. V. Snezhkina, D. V. Kalinin, A. V. Pokrovsky, A. L. Golovyuk, O. A. Stepanov, E. A. Pudova, G. S. Razmakhaev, M. V. Orlova, A. P. Polyakov, M. V. Kiseleva, A. D. Kaprin, A. V. Kudryavtseva

“…Paired histologically normal tissues or blood were unavailable, so potentially germline mutations were excluded from the analysis with strong filtering conditions using 1000 Genomes Project and ExAC databases. …”
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Pneumonia among Under-Five Children in Northwest Ethiopia: Prevalence and Predictors—A Community-Based Cross-Sectional Study by Zewudu Andualem, Tsegaye Adane, Abiye Tigabu, Walelign Worku Yallew, Sintayehu Daba Wami, Henok Dagne, Jember Azanaw, Gebisa Guyasa, Zelalem Nigussie Azene, Mastewal Endalew

“…However, evidence dealing with the problem is still unavailable. The current study is aimed at determining the magnitude and risk factors of childhood pneumonia in Gondar City. …”
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Comparable outcomes after busulfan- or treosulfan-based conditioning for allo-HSCT in children with ALL: results of FORUM by Krzysztof Kalwak, Laura M. Moser, Ulrike Pötschger, Peter Bader, Katharina Kleinschmidt, Roland Meisel, Jean-Hugues Dalle, Akif Yesilipek, Adriana Balduzzi, Gergely Krivan, Evgenios Goussetis, Raquel Staciuk, Petr Sedlacek, Herbert Pichler, Peter Svec, Melissa Gabriel, Tayfun Güngör, Ernest Bilic, Jochen Buechner, Marleen Renard, Kim Vettenranta, Marianne Ifversen, Cristina Diaz-de-Heredia, Jerry Stein, Jacek Toporski, Marc Bierings, Christina Peters, Marc Ansari, Franco Locatelli

“…Abstract: The superiority of total body irradiation (TBI)–based vs chemotherapy conditioning for allogeneic hematopoietic stem cell transplantation (allo-HSCT) in children with acute lymphoblastic leukemia (ALL) has been established in the international, prospective phase-3 FORUM study, randomizing 417 patients aged 4-18 years in complete remission (CR), who received allo-HSCT from HLA-matched sibling or unrelated donors. Because of the unavailability of TBI in some regions and to accommodate individual contraindications, this study reports the prespecified comparison of outcomes of patients receiving busulfan (BU)– or treosulfan (TREO)–based regimens from 2013 to 2018. …”
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Arbovirus vectors of epidemiological concern in the Americas: A scoping review of entomological studies on Zika, dengue and chikungunya virus vectors. by Reilly Jones, Manisha A Kulkarni, Thomas M V Davidson, RADAM-LAC Research Team, Benoit Talbot

“…<h4>Results</h4>A total of 96 studies were included in the scoping review after initial screening and subsequent exclusion of out-of-scope studies, secondary data publications, and studies unavailable in English language.<h4>Key findings</h4>We observed a steady increase in number of publications, from 2013 to 2018, with half of all studies published from January 2017 to March 2018. …”
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Early assessment of IL8 and PD1+ Treg predicts response and guides treatment monitoring in cemiplimab-treated cutaneous squamous cell carcinoma by Teresa Troiani, Fabiana Napolitano, Luigi Formisano, Roberto Bianco, Daniela Russo, Daniela Esposito, Giuseppe Ercolano, Angela Ianaro, Alberto Servetto, Daniela Claudia Maresca, Marcella Scala, Annarita Amato, Stefania Belli, Claudia Maria Ascione, Angela Vallefuoco, Giovanna Attanasio, Fabio Somma, Silvia Varricchio, Massimo Mascolo, Claudia Costa, Alessia Villani, Massimiliano Scalvenzi, Gianfranco Orlandino

“…Purpose Anti-programmed cell death 1 (PD1) is the first-choice treatment in patients with advanced cutaneous squamous cell carcinoma (cSCC), when curative options are unavailable. However, reliable biomarkers for patient selection are still lacking.Experimental design In this translational study, clinical annotations, tissue and liquid biopsies were acquired to investigate the association between sustained objective responses and transcriptional profiles, immune cell dynamics in tumor tissue and peripheral blood samples, as well as circulating cytokine levels.Results First, we investigated the baseline characteristics of the immune landscape of cSCC biopsies. …”
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COVID-19 status and utilisation of essential maternal and child healthcare services during the pandemic in Ahmedabad, India by Sandul Yasobant, K Shruti Lekha, Ravina Tadvi, Bhavin Solanki, Walter Bruchhausen, Deepak Saxena

“…Social and health system determinants for disrupting healthcare services during the pandemic were fear of infection and unavailability of the health workforce. Health planners and policymakers are encouraged to take into consideration of these findings while building resilient health care for managing future pandemics.…”
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Developing a Machine Learning–Based Automated Patient Engagement Estimator for Telehealth: Algorithm Development and Validation Study by Pooja Guhan, Naman Awasthi, Kathryn McDonald, Kristin Bussell, Gloria Reeves, Dinesh Manocha, Aniket Bera

“…Hence, the typical patient engagement behaviors are now different, and health care provider training on telehealth patient engagement is unavailable or quite limited. Therefore, we explore the application of machine learning for estimating patient engagement. …”
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Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy by Matthias Katschnig, Boris Maroh, Natascha Andraschek, Sandra Schlögl, Ulrike Zefferer, Elisabeth Bock, Gerd Leitinger, Christa Trattnig, Maria Kaufmann, Werner Balika, Clemens Holzer, Ute Schäfer, Silke Patz

“…Whilst the significance of substrate topography as a regulator of cell function is well established, a systematic analysis of the principles underlying this is still unavailable. Here we evaluate the hypothesis that surface energy plays a decisive role in substrate-mediated modulation of cell phenotype by evaluation of cell behaviour on synthetic microstructures exhibiting pronounced differences in surface energy. …”
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Development of the Big Ten Academic Alliance Collaborative for Women in Medicine and Biomedical Science: “We Built the Airplane While Flying It” by Maya S Iyer, Aubrey Moe, Susan Massick, Jessica Davis, Megan Ballinger, Kristy Townsend

“…Despite accumulating evidence that career development programming for women+ is critical for professional advancement and well-being, accessibility of these programs is generally limited to small cohorts, only offered to specific disciplines, or otherwise entirely unavailable. Opportunities for additional, targeted career development activities are imperative in developing and retaining women+ faculty. …”
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Evaluation of the impact of the state of emergency during the COVID-19 pandemic on childhood immunizations in Benguela Province, Angola by Tadatsugu Imamura, Keiji Mochida, Balogun Olukunmi, Lino Tchicondingosse, Pedro Sapalalo, Ketha Rubuz Francisco, Ai Aoki, Akira Ishiguro, Kenji Takehara

“…The most common reason for missing immunization was vaccine unavailability at health units (19.9%, 684/3,440). Less than 10% of missed immunizations were due to the SoE, which occurred mostly in infants born in rural municipalities in February 2020 (9.8%, 52/532). …”
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Using the optimal seed germination temperature approach to determine the potential distribution of Inga jinicuil in Mexico under climate change scenarios by Salvador Sampayo-Maldonado, Daniel Cabrera-Santos, Patricia Dávila-Aranda, Norma Isela Rodríguez-Arévalo, Alma Orozco-Segovia, Maraeva Gianella, Elizabeth Bell, Michael Way, Robert H. Manson, Gabina S. Quintas, Cesar M. Flores-Ortíz, Tiziana Ulian

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Qualitative Insights on Barriers to Receiving a Second Dose of Measles-Containing Vaccine (MCV2), Oromia Region of Ethiopia by Kalkidan Solomon, Brooke N. Aksnes, Abyot Bekele Woyessa, Chala Gari Sadi, Almea M. Matanock, Monica P. Shah, Paulos Samuel, Bekana Tolera, Birhanu Kenate, Abebe Bekele, Tesfaye Deti, Getachew Wako, Amsalu Shiferaw, Yohannes Lakew Tefera, Melkamu Ayalew Kokebie, Tatek Bogale Anbessie, Habtamu Teklie Wubie, Aaron Wallace, Ciara E. Sugerman, Mirgissa Kaba

“…Barriers among caregivers included lack of knowledge and awareness about MCV2 and the vaccination schedule, competing priorities, long wait times at health facilities, vaccine unavailability, negative interactions with HCWs, and transportation challenges. …”
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Assessment of Asthma Control and Quality of Life among Asthmatic Patients Attending Armed Forces Referral and Teaching Hospital, Addis Ababa, Ethiopia by Tesfalidet Gebremeskel Zeru, Ephrem Engidawork, Alemseged Beyene Berha

“…However, poor patient-physician communication, unavailability of appropriate medications, and lack of long-term goals have made asthma control difficult in developing countries. …”
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Novel image registration algorithm for scene-matching navigation by Hongrui YANG, Qiju ZHU, Peixian CAO, Hao GU, Dongdong ZHAO

“…In scenarios where satellite navigation is unavailable, image matching–based visual navigation technology becomes essential owing to its simple device structure and high accuracy in passive positioning. …”
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Healthcare practitioners as accomplices: a qualitative study of gender affirmation in a context of ambiguous regulation in Indonesia by Benjamin Hegarty, Alegra Wolter, Amalia Puri Handayani, Kevin Marian, Jamee Newland, Dede Oetomo, Ignatius Praptoraharjo, Angela Kelly-Hanku

“…Given that all participants expressed a desire for gender affirmation, but such services were widely unavailable, accomplices played a crucial role in supporting trans people to access healthcare. …”
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Attention to Monkeypox: An Interpretable Monkeypox Detection Technique Using Attention Mechanism by Avi Deb Raha, Mrityunjoy Gain, Rameswar Debnath, Apurba Adhikary, Yu Qiao, Md. Mehedi Hassan, Anupam Kumar Bairagi, Sheikh Mohammed Shariful Islam

“…This helps better distinguish monkeypox from other similar skin diseases, particularly in its early stages or when a detailed medical examination is unavailable. To ensure transparency and interpretability, we incorporated Gradient-weighted Class Activation Mapping (Grad-CAM) and Local Interpretable Model-Agnostic Explanations (LIME) to provide clear insights into the model&#x2019;s diagnostic reasoning. …”
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