Shaoyao Gancao decoction alleviates paclitaxel-induced cognitive impairment by activating PTEN/PI3K/AKT pathway to inhibit NETs formation by Xu Ying, Su Yue, Hu Yuwen, Li Xiang, Zhou Ziyan, Yuan Ningning, Ji Xiaowei, Jiang Ruoyu, Wang Wenzhu, Zhang Yafeng, Zhai Guojie, Cheng Xiaolan

“…The impact of SGD on inflammatory factors, microglia activation and NETs formation were also studied by ELISA and immunofluorescence. The regulation of SGD on PTEN/PI3K/AKT pathway was detected by Western blot. …”
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β-synuclein in cerebrospinal fluid as a potential biomarker for distinguishing human prion diseases from Alzheimer’s and Parkinson’s disease by Bing Xu, Kang Xiao, Xiaoxi Jia, Rundong Cao, Donglin Liang, Ruhan A, Weiwei Zhang, Chunjie Li, Liping Gao, Cao Chen, Qi Shi, Xiaoping Dong

“…The CSF β-syn levels were measured by a commercial microfluidic ELISA. The Mann–Whitney U test and Kruskal–Wallis H test were employed to analyze two or more sets of continuous variables. …”
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IL-6/GATA2/SERPINE1 pathway is implicated in regulating cellular senescence after acute kidney injury by Hongshuang Su, Xiaoxi Lin, Ayinuer Paredong, Ayinuer Paredong, Congcong Yao, Yan Zhang, Mengke Geng, Yuqian Guan, Lichao Gong, Feng Jiang, Qi Lv, Songtao Shou, Heng Jin

“…Additionally, the levels of IL-6 were detected by employing ELISA kits. RNA sequencing analysis was performed on cells treated with myoglobin and tocilizumab. …”
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Saussurea gossypiphora extracts reduce inflammation by reversing inflammatory cytokine levels in in-vitro and in-vivo by Kalyani Jatoth, Pawan Kumar Anoor, Ramesh Kande, Sandeepta Burgula

“…Effect of MESGD on inflammasome pathway was studied by performing ELISA for IL-1β, TNF-α and confirmed by immunoblotting for NLRP3 and Caspase 1 in THP1 cells. …”
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Inhibition of complement system-related gene ITGB2 attenuates epithelial–mesenchymal transition and inflammation in diabetic nephropathy by Jun Peng, Wenqi Zhao, Lu Zhou, Kun Ding

“…Then the cell viability, inflammatory factors, and the expression of epithelial–mesenchymal transition (EMT) and fibrosis markers were determined by using Cell Counting Kit-8 (CCK-8) assay, enzyme linked immunosorbent assay (ELISA), western blotting assays, respectively. Results In total, 1012 DEGs and 974 DN-related genes were screened, and intersection analysis of the three (DN-related genes, DEGs and CSRGs) yielded 13 intersection genes, which were considered as the DCSRGs. …”
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FBR2 modulates ferroptosis via the SIRT3/p53 pathway to ameliorate pulmonary fibrosis by Yu Cheng, Yang Jiao, Wan Wei, Mengjia Kou, Yaodong Cai, Yang Li, Hao Li, Tonghua Liu

“…Assessments encompassed histopathology, ELISA for cytokine detection, IHC and Western blot for protein expression analysis, and qRT-PCR for gene expression quantification. …”
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Chemopreventative potential of Salsola tuberculatiformis Botch and Compound A in the differential regulation of IL-1Ra:IL-1α in UVB exposed skin keratinocytes by T. Magcwebeba, L. McEvoy, S. Hayward, T. Tait, J.L Quanson, L. Kelbe, WCA Gelderblom, M. Lilly, A.C Swart, P. Swart

“…Anti/pro-inflammatory effects were evaluated by measuring IL-1α and IL-1Ra production using an ELISA assay. Additionally, the study monitored cell viability through ATP production, apoptosis via Caspase-3 activity, and oxidative stress using the DCFDA assay. …”
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Human cytomegalovirus (HCMV) trends in Sri Lanka: insights from a hospital-based seroprevalence analysis by Narapity Pathirannehalage Sunil-Chandra, Madawala Vithanage Madusha Lakmali Jayasundara, Maththe Gama Ralalage Shobha Sanjeewani Gunathilaka, Suduwa Dewage Himal Suminda

“…Methods A total of 820 blood samples obtained from 820 participants of selected low and high-risk populations in Sri Lanka, namely; hospital inpatients (n = 200), antenatal women (n = 80), blood donors (n = 80), cancer patients (n = 200), Sexually Transmitted Disease (STD) clinic attendees (n = 160) and Chronic Kidney Disease (CKD) patients on haemodialysis (n = 100) were tested for IgG antibodies against HCMV by Enzyme Linked Immunosorbent Assay (ELISA). Statistical analysis was performed using IBM SPSS Statistics version 30 (IBM® Corporation, New York, USA). …”
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Analisis Tingkat Kepuasan Pengguna Aplikasi Mytelkomsel Menggunakan Evaluasi Heuristik dan Metode Pieces (Studi Kasus : Mahasiswa Kampus Karawaci Universitas Gunadarma) by Fajar Nugraha, Dewi Agushinta Rahayu, Irwan Bastian

“…Tujuan aplikasi ini untuk memberikan informasi tentang sisa pulsa, kuota internet yang aktif, masa aktif kartu Subscriber Identity Module (SIM), dan masih banyak lagi. …”
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Dietary supplementation of Astragalus polysaccharide or its nanoparticles enhances testicular hemodynamics, echotexture, scrotal circumference, concentration of testosterone, estra... by Eman Fayez, Ali Salama, Mohamed Ahmed Ismail, Fady Sayed Youssef, Zaher Mohamed Rawash, MR Oshba, Haney Samir

“…Serum testosterone (T) and estradiol (E2) concentrations were determined by ELISA kits, while nitric oxide (NO) and total antioxidant capacity (TAC) were measured spectrophotometrically. …”
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Antioxidant and Anti-Inflammatory Potential of Cymbopogon nardus Ethanol Extract on 3T3-L1 Cells by Rohmawaty E, Wiraswati HL, Zahra TA, Amalina SN, Ramadhanti J, Rosdianto AM, Laelalugina A, Nasution GTD, Kamisah Y

“…Enny Rohmawaty,1 Hesti Lina Wiraswati,1 Tamara Aliya Zahra,2 Shabrina Nur Amalina,2 Julia Ramadhanti,1 Aziiz Mardanarian Rosdianto,1 Amila Laelalugina,3 Gita Tiara Dewi Nasution,1 Yusof Kamisah4 1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, 40161, Indonesia; 2Undergraduate Program Medical Doctor, Faculty of Medicine, Universitas Padjadjaran, Bandung, 40161, Indonesia; 3Oncology and Stem Cell Working Group, Universitas Padjadjaran, Bandung, 40161, Indonesia; 4Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, 53000, MalaysiaCorrespondence: Enny Rohmawaty, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jl. …”
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Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils by Yixiang Jiang, Ning Wang, Jingyi Liu, Haoran Ren, Wenkang Jiang, Yanting Lei, Xidan Fu, Miao Hao, Xiujuan Lang, Yumei Liu, Xijun Liu, Rui Li, Hulun Li

“…In vitro, flow cytometry and ELISA measured the effects of hMSC-L and hMSC-M on N2 polarization and inflammatory cytokines expression in primary murine neutrophils. …”
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Effects of coal mine dust on lung function in rats by LIU Yang, LI Meng, LU Liyuan, WANG Ru, YANG He, ZHANG Huifang

“…Lung tissues were collected to measure reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels using corresponding ELISA kits and ATP assay kits, respectively. The relative mRNA expressions of peroxisome proliferators-activated receptor gamma coactivator 1-alpha (PGC-1α) and mitochondrial transcription factor A (TFAM) were detected using real-time fluorescent quantitative polymerase chain reaction assay. …”
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Network Pharmacology and Experimental Validation of the Therapeutic Effect of Baji Capsule on LPS-Induced Osteoporosis by Li Q, Li D, Tian C, Liu X, Wang H, Liu H

“…The therapeutic effect of Baji capsule was evaluated by high-resolution micro-computed tomography (Micro-CT) and tissue section staining, serum inflammatory factor levels were assessed by ELISA, serum oxidative stress-related marker levels were determined by kits, and finally the mechanism was explored by network pharmacology and then verified by immunohistochemistry.Results: Micro-CT results showed that Baji capsule attenuated LPS-induced inflammatory bone loss.Baji capsule also reduced serum inflammatory factor levels and oxygen free radical production. …”
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Emodin protects against severe acute pancreatitis-associated acute lung injury by activating Nrf2/HO-1/GPX4 signal and inhibiting ferroptosis in vivo and in vitro by Gang Shen, Haiyun Wen, Huijuan Li, Xuetao Zhang, Bowen Lan, Xuanchi Dong, Peng Ge, Yalan Luo, Hailong Chen

“…Enzyme-linked immunosorbent assays (ELISA) were used to assess the levels of pro-inflammatory cytokines, Fe2+, and associated oxidative stress indicators in the serum and cell supernatant. …”
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Therapeutic efficacy and mechanism of artesunate for mouse model of polycystic ovary syndrome by WANG Xueling, WANG Xueling, ZHONG Peiling, ZHONG Peiling, ZHAO Zhipeng

“…After modeling, AS of 15 and 30 mg/kg was intraperitoneally injected into the mice of the AS15 and AS30 groups, respectively, and 200 mg/kg Met was given to those of the Met group by gavage, once per day, for 6 weeks. ELISA was used to detect serum testosterone (T), fasting insulin (FINS), luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and the LH/FSH ratio was calculated. …”
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Behavioral representations within the endogenous dual attentional pathways during audiovisual integration processing by Zhongtian Guan, Mingli Yan, Miao He, Yubo Liu, Zhixi Zhang, Chunlin Li

“…Linear integrated speed–accuracy score (LISAS) and balanced integration score (BIS) showed different speed-accuracy balance in our task.ConclusionOur findings emphasize the synergy of functions and the dynamic synergy of cognitive processes in dorsal and ventral attentional pathways, which contribute to the management of cognitive flexibility and efficiency.…”
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Dual Role of α-MSH in Colitis Progression: Mediating Neutrophil Differentiation via Bone Marrow by Liao X, Liu H, Li Y, Zhang W, Dai Q, Wei H, Zhou J, Xie X, Zhou H

“…This study aims to investigate the alterations in the HPA and its potential roles during IBD development.Methods: We utilized a dextran sodium sulfate (DSS)-induced colitis model in mice and employed immunofluorescence, real-time quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), among other techniques, to evaluate the impact of colitis on the HPA. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Enzyme-linked immunosorbent assay (ELISA), Reverse transcription - quantitative polymerase chain reaction (RT-qPCR), Western blotting (WB), immunohistochemistry, and immunofluorescence were used to detect NLRP3 inflammasome activation in vivo. …”
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Association of Serum Irisin With Severity and Prognosis in Patients With Coronavirus Disease 2019: A Prospective Cohort Study by Zhang X, Wang W, Li Y, Liu X, Zhang L, Cheng Z, Liu M, Wang Y, Fu L, Yang J

“…The level of serum irisin was determined by ELISA. The associations of serum irisin with COVID-19 severity and prognosis were evaluated through linear and logistic regression models on the basis of a prospective cohort study.Results: Serum irisin levels were lower in severe patients than in mild patients. …”
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