KLF5 silencing restrains proliferation, invasion, migration and angiogenesis of gallbladder carcinoma cells by transcriptional regulation of PDGFA by Xiaowei Lu, Kui Hu, Dandan Zhang, Xuefeng Yin, Jifeng Nie, Kai Zhao

“…Angiogenesis was assessed by ELISA, tube formation assay and western blot. The interaction between KLF5 and PDGFA was confirmed by ChIP assay, as well as luciferase reporter assay. …”
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Staphylococcus aureus induces mitophagy via the HDAC11/IL10 pathway to sustain intracellular survival by Yaji Yang, Haotian Zhou, Feilong Li, Yanhao Zhang, Jianye Yang, Yidong Shen, Ning Hu, Quanming Zou, Leilei Qin, Hao Zeng, Wei Huang

“…Methods This study employed confocal microscopy, flow cytometry, ELISA, and siRNA technology to assess the survival capacity of S. aureus within macrophages and the impact of inflammatory cytokines on its persistence. …”
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Activin levels correlate with lymphocytic infiltration in epithelial ovarian cancer by Elizabeth T. Evans, Emily F. Page, Alex Seok Choi, Zainab Shonibare, Andrea G. Kahn, Rebecca C. Arend, Karthikeyan Mythreye

“…Immunohistochemical analysis was performed to evaluate activin A and T‐cell markers CD8 and FoxP3 at the protein level. ELISA to activin‐A was used to assess levels in the ascites of advanced EOC patients. …”
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Ginkgetin delays the progression of osteoarthritis by inhibiting the NF-κB and MAPK signaling pathways by Liang Zhu, Yanchi Bi, Ting Liang, Po Zhang, Xiao Xiao, Tengbo Yu

“…GK's anti-inflammatory and antioxidative effects were comprehensively assessed via MTT assay, western blot, cell immunofluorescence, ELISA, and transcriptome sequencing. Potential underlying mechanisms were also explored. …”
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Bradykinin B1 receptor signaling triggers complement activation on endothelial cells by Ingrid Lopatko Fagerström, Alexandra Gerogianni, Markus Wendler, Ida Arvidsson, Ashmita Tontanahal, Ann-Charlotte Kristoffersson, Fatimunnisa Qadri, Michael Bader, Michael Bader, Michael Bader, Michael Bader, Michael Bader, Diana Karpman

“…Complement factors C3a, factor Ba and C5b-9 were evaluated by ELISA or immunoblotting. B1/B2 receptor double knock-out and wild-type mice were injected with lipopolysaccharide from E. coli B5:O55, to induce KKS activation.ResultsSupernatants from PGECs incubated with serum exposed to kaolin or FXIIa exhibited higher levels of Ba and C5b-9, which were significantly reduced in the presence of the B1R antagonist. …”
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Development and evaluation of a novel E7 multi-epitopic vaccine for human papillomavirus type 16: design, expression, purification, and immunological characterization by Bahareh Bahmani, Zahra Amini-bayat, Mohammad Mehdi Ranjbar, Masoud Hassanzadeh Makoui, Amir-Hassan Zarnani

“…Polyclonal antibodies were generated, and their reactivity with immunizing antigen and native protein in E7 expressing cells (TC-1) was assessed by ELISA and immunofluorescent staining, respectively. …”
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Clofarabine induces tumor cell apoptosis, GSDME-related pyroptosis, and CD8+ T-cell antitumor activity via the non-canonical P53/STING pathway by Jing Chen, Jie Wu, Xiang Chen, Qian Tao, Nian Liu, Cong Peng, Can Lu, Qiuqiu Li

“…The molecular mechanisms of Clo-induced tumor cell death were analyzed using western blotting, ELISA, reverse transcription-PCR, immunofluorescence, co-immunoprecipitation (CO-IP), short hairpin RNA, co-culture, chromatin immunoprecipitation, and flow cytometry. …”
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Diagnostics and treatment of Clostridium difficile-associated disease: Guidelines of the Russian gastroenterological association by V. T. Ivashkin, N. D. Yushchuk, I. V. Mayev, Tatyana L Lapina, Ye. A. Poluektova, O. S. Shifrin, A. S. Tertychny, A. S. Trukhmanov, A. A. Sheptulin, Ye. K. Baranskaya, O. S. Lyashenko, K. V. Ivashkin

“…At the present time several laboratory methods for diagnosis of Clostridial infection exist: fecal ELISA test for toxins A and B, PCR, test for C. difficile glutamate dehydrogenase. …”
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MsCL-P1: A multifunctional C-type lectin from largemouth bass (Micropterus salmoides) with broad microbe binding and agglutination activities by Siyi She, Ying Ye, Fengyao Guo, Shun Yang, Mengmeng Huang, Hui Fei

“…The recombinant CRD of MsCL-P1 (rCRD) demonstrated binding activity to various pathogen-associated molecular patterns (PAMPs), including lipopolysaccharide (LPS), peptidoglycan (PGN), and glucan (GLU), as determined by ELISA. Furthermore, the rCRD showed significant binding activity towards multiple microbes, including Gram-positive bacteria, Gram-negative bacteria, and fungi. …”
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Intravenous versus subcutaneous tocilizumab in Takayasu arteritis: multicentre retrospective study by Patrice Cacoub, Arsène Mekinian, David Saadoun, Pavel I Novikov, Savino Sciascia, Corrado Campochiaro, Olivier Fain, Ilya Smitienko, Nicolas Schleinitz, Patrick Jégo, Francesco Muratore, Carlo Salvarani, Elena Galli, Sabine Berthier, Marc Lambert, François Maurier, Isabelle Kone-Paut, Sergey Moiseev, Masataka Kuwana, Alexandre Belot, Martin Michaud, Francis Gaches, Achille Aouba, Xavier Puechal, Karim Sacre, Tiphaine Goulenok, Alessandro Tomelleri, Thomas Sené, Elena Marina Baldissera, Luigi Boiardi, Abid Awisat, Ygal Benhamou, Vahan Mukuchyan, Mathieu Vautier, Azeddine Dellal, Lucie Biard, Julie Seguier, José Hernández-Rodríguez, Olivier Espitia, Sebastien Humbert, Guillaume Denis, Nolan Hassold, Dagna Lorenzo, Helene Munoz Pons, Jean Baptiste Gaultier, Le Mouel Edwige, Antoinette Perlat, Bertrand Lioger, Jonathan Broner, Virginie Dufrost, Faten Frikha, Alexandra Audemard-Verger, Pascal Woaye-Hune, Pierre Zeminsky, Moya Alvarado, Matheus Vieira, Alberto Lo Gullo

“…Objectives In this large multicentre study, we compared the effectiveness and safety of tocilizumab intravenous versus subcutaneous (SC) in 109 Takayasu arteritis (TAK) patients.Methods We conducted a retrospective multicentre study in referral centres from France, Italy, Spain, Armenia, Israel, Japan, Tunisia and Russia regarding biological-targeted therapies in TAK, since January 2017 to September 2019.Results A total of 109 TAK patients received at least 3 months tocilizumab therapy and were included in this study. …”
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Selecting the Best Music for the Moment in a Music and Imagery Session by Petra Jerling, Carmen Angulo Sánchez-Prieto, Isabel Solana Rubio

“… Music and Imagery (MI) is a receptive music therapy method within the Continuum Model of Guided Imagery and Music developed by Lisa Summer. In receptive music therapy, clients listen to music for therapeutic purposes. …”
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Estimating Willingness to Pay for New Mandarin Cultivars: A Revealed Preference Approach by Xiang Bi, Lisa House, Zhifeng Gao, Frederick Gmitter

“…This 6-page fact sheet was written by Xiang Bi, Lisa House, Frederick Gmitter, and Zhifeng Gao, and published by the UF Department of Food and Resource Economics, September 2014. …”
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Estimating Willingness to Pay for New Mandarin Cultivars: A Revealed Preference Approach by Xiang Bi, Lisa House, Zhifeng Gao, Frederick Gmitter

“…This 6-page fact sheet was written by Xiang Bi, Lisa House, Frederick Gmitter, and Zhifeng Gao, and published by the UF Department of Food and Resource Economics, September 2014. …”
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Developing a risk score using liquid biopsy biomarkers for selecting Immunotherapy responders and stratifying disease progression risk in metastatic melanoma patients by Amalia Azzariti, Simona De Summa, Tommaso M. Marvulli, Ivana De Risi, Giuseppe De Palma, Roberta Di Fonte, Rossella Fasano, Simona Serratì, Sabino Strippoli, Letizia Porcelli, Michele Guida

“…Methods Soluble forms of PD1, PD-L1, LAG-3, CTLA-4, CD4, CD73, and CD74 were quantified using ELISA assay in plasma of a cohort of 110 MM patients, at baseline, to investigate possible correlations with clinical outcomes. …”
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Revealing viral hepatitis epidemiology in the Democratic Republic of Congo: insights from yellow fever surveillance reanalysis by Patrick Mukadi-Kakoni, Yannick Munyeku-Bazitama, Gracia Kashitu-Mujinga, Marguerite Manwana-Pemba, Niclette Zenga-Bibi, Patient Okitale-Talunda, Christelle Mbelu-Kabongo, Fleurette Domai-Mbuyakala, Elisabeth Pukuta-Simbu, Pierre Mutantu-Nsele, Yoshinao Kubo, Sheila Makiala-Mandanda, Steve Ahuka-Mundeke, Koya Ariyoshi, Jean-Jacques Muyembe-Tamfum

“…Samples from individuals testing negative for yellow fever were tested for IgM antibodies against HAV, HBc, HCV, and HEV and HBs antigen using validated ELISA kits. Acute HBV infection was defined by both HBc IgM and HBs antigen positivity. …”
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Immunogenicity of Two Fractional Inactivated Poliovirus Vaccine Doses vs Single Intramuscular Inactivated Poliovirus Vaccine Dose in Infants of Low- and Middle-income Countries: A... by Kunal Kumar, Sangita Yadav, Raghvendra Singh, Archana Thakur

“…The study measured the levels of antibodies using human poliovirus (PV) antibody immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) kits and identified seroprotection as the presence of antibody >1:8 dilution. …”
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The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind,... by Mehran Noori, Zainab Shateri, Siavash Babajafari, Mohammad Hadi Eskandari, Karim Parastouei, Mohammad Ghasemi, Hoseein Afshari, Mohammad Samadi

“…Oxidative stress parameters were assessed using the standard calorimetric method, and inflammatory parameters were measured via the enzyme-linked immunosorbent assay method (ELISA). The differences between the two groups were compared using the independent samples T-test. …”
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Identification of novel pQTL‐SNPs associated with lung adenocarcinoma risk: A multi‐stage study by Yutong Wu, Huiwen Xu, Liping Mao, Rongrong Zhao, Junfeng Chu, Lili Huang, Wendi Zhang, Yiran Liu, Qiong Chen, Xiaobo Tao, Siqi Li, Shenxuan Zhou, Anhui Ning, Zhenyu Li, Tian Tian, Lei Zhang, Jiahua Cui, Guangyu Tian, Minjie Chu

“…Enzyme linked immunosorbent assay (ELISA) is used to determine the levels of candidate protein. …”
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The diagnostic performance evaluation of the SD BIOLINE HIV/syphilis Duo rapid test in southern Ethiopia: a cross-sectional study by Techalew Shimelis, Endale Tadesse

“…Where the result of the STAT-PAK is discordant with KHB, Unigold HIV is used as a tiebreaker to determine the result. We also used ELISA to resolve discordant HIV results. Syphilis serostatus was determined using the Treponema pallidum haemagglutination assay (TPHA).Results The respective sensitivity, specificity, positive predictive value and negative predictive value of the SD BIOLINE HIV/syphilis Duo test were 100, 99.5, 99.5 and 100% for HIV and 97.6, 96, 95.4 and 98% for syphilis testing, respectively. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…Enzyme-linked immunosorbent assays (ELISA) assessed the levels of oxidative stress, antioxidants, and apoptosis-related proteins in the kidney tissue. …”
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