Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite by Mahsa Mirzayi, Balal Khalilzadeh, Sattar Akbari Nakhjavani, Rahim Mohammad-Rezaei

“…The results of the studies projected antibody (Ab) CA-19-9/AgNPs-MXene electrochemical immunosensor as a potential and affordable alternate of conventional methods like ELISA. The desirable LOD was recorded as 1 nU/mL. …”
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Differences in HIV infection trends in two regions of Cameroon with a longstanding HIV epidemic: insights from 2012 and 2022 by Yannick F. Ngoume, Urmes C. Teagho, Brice Eselacha, Oumarou H. Goni, Dell-Dylan Kenfack, Mérimé Tchakoute, Georges Nguefack-Tsague, Marcel Tongo, Marcel Tongo

“…In this regard, we determined the HIV prevalence in remote communities of the East and South administrative regions of Cameroon where the four cross-species SIV transmissions that gave rise to the four HIV-1 groups likely happened.MethodsWe did this in two different periods: 2012/2013 (Period 1; 4,435 participants enrolled in the East and 2,347 in the South) and 2021/2022 (Period 2; 2,203 participant enrolled in the East and 2,347 in the South) using HIV1/2 rapid assays and standard ELISAs and according to the WHO testing strategy.ResultsDuring Period 1, the HIV seroprevalence in the East was 6.9%. …”
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A Study on the Role of LPA and ATX in Breast Cancer in Iraq by Anfal Akeel Taha, Hind Salman Jassim, Wafaa Fadhil Hamad

“…Blood samples were collected, and biochemical parameters, including LPA and ATX levels, were measured using the ELISA technique. Statistical analyses were performed with SPSS version 24, and receiver operating characteristic (ROC) analysis was used to evaluate diagnostic capabilities. …”
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Study on clinically manifested reproductive health problems of dairy cows managed under intensive and semi-intensive production systems in Wondo Genet district, Southern Ethiopia by Amene Fekadu, Yifat Denbarga, Tesfaye Belay, Desie Sheferaw, Fufa Abunna, Kebede Amenu, Rahmeto Abebe, Mesele Abera, Berhanu Mekibib

“…This study also demonstrated that none of the serum samples collected from Wondo Genet and other towns (namely Hawassa, Wolaita Sodo and Arsi Negelle) and tested using Indirect Multi-species ELISA was positive for bovine brucellosis. Further studies, preferably laboratory based, should be conducted to identify the underlined causes of these problems in different parts of the country. …”
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Possible erythropoietin pharmacotherapeutic targets on painful diabetic neuropathy in rats by SAMUEL SUAREZ-MENDEZ, DEYSI Y. BERMÚDEZ-OCAÑA

“…To determine the action mechanisms of erythropoietin, levels of NF-κB in serum were measured with ELISA and was used L-NAME (Nω-nitro-L-arginine methyl ester hydrochloride, non-selective nitric oxide synthase inhibitor; 0.1-1 mg/kg, i.p.), glibenclamide (ATP-sensitive K+ channels blocker; 1-10 mg/kg, i.p.), methiothepin (non-selective 5-HT receptor antagonist; 0.01-0.1 mg/kg, i.p.) and naloxone (non-selective opioid receptor antagonist; 1 mg/kg). …”
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Challenges in HIV Diagnosis Algorithm: Experience of the Confirmation Laboratory by Özgür Appak, Derya Özarslan, Arzu Nazlı, Ayca Arzu Sayiner

“…An important factor was that clinicians did not order HIV-1-RNA-PCR from ELISA reactive and Geenius test negative patients. …”
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Clinical and Morphological Manifestations of Gastritis and Serum Cytokine Levels in Schoolchildren with Familial History of Gastric Cancer by T. V. Polivanova, E. V. Kasparov, V. A. Vshivkov

“…Serum cytokine levels (IL-2, IL-4, IL-8, IL-18, IL-1β, IFN-α, TNM-α) were obtained in enzyme immunoassays (ELISA).Results. Schoolchildren with gastritis and familial history of gastric cancer revealed a higher 59.8% rate of dyspeptic complaints vs. 40.8% in negative history (p = 0.001), as well as complaints of weekly heartburn in 14.2 and 8.3% cas-es (p = 0.019), respectively. …”
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Neuroprotective Potentials Of Extracts From Moringa Oleifera And Musa Sapientum Against Cadmium Chloride-Induced Neurotoxicity In Rats Cerebri by Adelaja Akinlolu

“…Tissue concentrations of catalase, superoxide dismutase, cyclo-oxygenase-2 and cytochrome P450 in rats’ cerebri were determined using ELISA. Statistical analyses (p ≤ 0.05) of data were conducted using the Mann-Whitney U Test. …”
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Peri Cholecystectomy Bile Analysis for Fasciola hepatica Eggs in Sulaimani Teaching Hospital by Seerwan Hama Shareef, Taher Abdullah Hawramy, Osman jamal nasrulla, Karwn Hama amin, Dara Ahmed Mohammed, Raz Seerwan Hama

“…Diagnosis is made on clinical background with imaging studies or laboratory (like stool and examinations biliary aspirate, intradermal test or ELISA to detect antibody). It must be taken into consideration as newly emerging infestation in our country. …”
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Prevalence of Japanese encephalitis in pigs in Mainland China during 2000–2024: a systemic review and meta-analysis by Xue-Tong Liu, Li-Dong Jiang, Yu-Ting Lin, Ran Zhao, Qi Wang, Shu-Ying Zhang, Shu-Ying Zhang, Shu-Ying Zhang, Emad Beshir Ata, Xin Liu, Yuan Wang, Zi-Xuan Liu, Cui Xu, Ying Xiao, Yi-Fan Wang, Xue Leng, Qing-Long Gong, Rui Du, Rui Du, Rui Du

“…Serum samples showed the highest prevalence 38.1% (95% CI: 27.9–48.9), and ELISA testing had a higher detection rate 38.2% (95% CI: 24.5–52.9). …”
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Limited transmission of avian influenza viruses, avulaviruses, coronaviruses and Chlamydia sp. at the interface between wild birds and a free-range duck farm by Chloé Le Gall-Ladevèze, Benjamin Vollot, Julien Hirschinger, Laëtitia Lèbre, Rachid Aaziz, Karine Laroucau, Jean-Luc Guérin, Mathilde Paul, Julien Cappelle, Guillaume Le Loc’h

“…The presence of the four pathogens was tested by q(RT-)PCR, and the immunity of wild birds to AIV and Newcastle disease virus (NDV) was tested by ELISA. Wild birds were found to shed AIV and Chlamydia only, with a low prevalence (< 3%). …”
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Integrated analysis of proteomics and metabolomics in infantile epileptic spasms syndrome by Jun Chen, Xiaoqian Wang, Xueyi Rao, Huan Luo, Yajun Shen, Jing Gan

“…The study then expanded the sample size to 10 per group and validated the identified proteins through ELISA analysis. A total of 24 differential metabolites (12 upregulated and 12 downregulated) and 79 differential proteins (18 upregulated and 61 downregulated) were identified. …”
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EFFECTS OF GREEN TEA EXTRACT ON GRAAFIAN FOLLICLES AND SERUM17b-ESTRADIOL IN MONOSODIUM GLUTAMATE-EXPOSED RATS by Syami Yulianti et al

“…Graafian follicles were counted using light microscope while serum 17b-estradiol level was measured using ELISA kit and the data was statistically analyzed using one-way ANOVA. …”
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Integrated Management of Spot Blotch Disease of Wheat (Triticum aestivum L.) Caused by Bipolaris sorokiniana (Sacc.) Shoem by Deepak Kumar, Ramji Singh, Durga Prasad, Ajay Tomar

“…Seven micronutrients-calcium chloride, zinc sulphate+lime, ferrous sulphate+lime, boron, sulfur, molybdenum, and manganese sulphate+lime-four botanicals-leaf extracts of tulsi, neem, mentha, and garlic clove extract-four bioagents-Trichoderma harzianum SV-7, T. harzianum SV-28, T. harzianum IRRI-1, Pseudomonas fluorescens-and six fungitoxicants-azoxystrobin, difenoconazole, propiconazole, tebuconazole, azoxystrobin+tebuconazole, and carbendazim+mancozeb-were assessed against spot blotch of wheat in artificially inoculated field conditions. …”
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Exploration of the anti-inflammatory potential of Polygonum bistorta L.: protection against LPS-induced acute lung injury in rats via NF-ĸβ pathway inhibition by Sajida Parveen, Kashif ur Rehman Khan, Shahid Muhammad Iqbal, Hanan Y. Aati, Areej M. Al-taweel, Liaqat Hussain, Musaddique Hussain, Musaddique Hussain

“…Plant extract markedly restored LPS-induced hypoxemia, pulmonary edema, histopathological alterations, and leukocyte infiltration in the lung. ELISA testing on BALF found that the plant extract efficiently reinstated superoxide dismutase, total anti-oxidant capacity, malondialdehyde, and total oxidative stress. qRT-PCR indicated a decline in the endotoxin-induced overproduction of pro-inflammatory markers, oxidative stress, transcription factor, and downregulated antioxidant potential in extract-treated groups. …”
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Does the Serum Vaspin Level Have a Role in the Diagnosis of Primary Ovarian Insufficiency? by Fatma Nur Koyun, Nafiye Yilmaz, Duygu Tugrul Ersak

“…Vaspin levels in serum samples were determined using the Enyzme-Linked ImmunoSorbent Assay (ELISA) method. RESULTS: The mean age of the patients with POI was 31.9±5.4 years, while it was 31.2±3.8 years in the control group. …”
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Toll-like receptor upregulation in liver and peripheral blood mononuclear cells of patients with amoebic liver abscess by Sandhya Khunger, Abhishek Mewara, Upninder Kaur, Ajay Duseja, Pallab Ray, Naveen Kalra, Navneet Sharma, Rakesh Sehgal

“…Serum cytokines level was quantified by flow cytometry. In-house ELISA for the analysis of IgG and its subtypes in the serum samples was performed. …”
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Salivary Levels of Interleukin-17 in Iranian Patients With Systemic Sclerosis by Ali Tadayon, Javad Moayedi, Mohamad Ali Nazarinia, Mahyar Malekzadeh, Sara Kazemi, Maryam Mardani

“…The salivary levels of IL-17 in all samples were measured using a commercially available enzyme–linked immunosorbent assay (ELISA) kit. The mean salivary levels of IL-17 in patients with SSc were significantly higher than the control group (199.6±38.9 pg/mL vs. 112.7±39.4 pg/mL, P<0.0001). …”
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Food addiction and associated factors in newly diagnosed patients with schizophrenia: a cross-sectional comparison with siblings and healthy controls by Feten Fekih-Romdhane, Youssef Boukadida, Abir Hakiri, Wided Homri, Majda Cheour, Souheil Hallit

“…Methods A cross-sectional study was conducted in Razi Hospital, Tunis, Tunisia, from January to June 2024. A total of 112 newly diagnosed, clinically stabilized patients with first-episode schizophrenia, 77 of their unaffected siblings and 78 healthy controls were included. …”
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Ruta alimentaria en circuitos y producción de traspatio en Donato Guerra, México by Celia Guzmán Hernández, Fabiola Soto Albarrán, Roberto Mendoza Vilchis, Andrés López Ojeda, Ricardo Hernández López

“…Objetivo: Diseñar una ruta alimentaria en circuitos considerando la actividad pecuaria de traspatio, sus recursos naturales y culturales en Donato Guerra, México para su aprovechamiento turístico.Metodología: Diagnóstico turístico que consistió en la aplicación de un cuestionario a 250 visitantes a través de un muestreo no probabilístico; 70 entrevistas con pobladores rurales; se hizo un inventario de sus patrimonios incluyendo un reconocimiento de infraestructura, servicios turísticos; y se empleó el método observacional para aspectos de medicina veterinaria y zootecnia (MVZ), se aplicó con el fin de conocer el manejo de las unidades de producción pecuaria.Resultados: La producción de traspatio se relaciona con los recursos agropecuarios locales y las recetas tradicionales que derivan de sus patrimonios culturales y naturales, lo cual constituye una fuente de consumo para los turistas, potencializándose a través del diseño de una ruta alimentaria con cinco circuitos, donde sus pobladores estén dispuestos a incorporar al turismo en sus actividades, y como una manera de conservar su entorno.Limitaciones: Los acuerdos entre todos los actores posponen acciones. …”
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