Integrative metabolomics and transcriptomics analysis of hippocampus reveals taurine metabolism and sphingolipid metabolism dysregulation associated with sleep deprivation-induced... by Ting Chen, Junke Jia, Chenyi Gao, Qi Zhong, Lijuan Tang, Xiaokai Sui, Shuang Li, Chang Chen, Zongze Zhang

“…Our results prompt severe metabolic disturbances occur in the hippocampus with sleep deprivation in mice, which can provide a basis for the mechanism research.…”
Get full text

De Novo Transcriptome Assembly of Rice Bean (<i>Vigna umbellata</i>) and Characterization of WRKY Transcription Factors Response to Aluminum Stress by Gunasekaran Ariharasutharsan, Manoharan Akilan, Manickam Dhasarathan, Manivel Amaravel, Sankaran Divya, Mariyappan Deivamani, Manickam Sudha, Muthaiyan Pandiyan, Adhimoolam Karthikeyan, Natesan Senthil

“…In our study, we performed a de-novo transcriptome assembly of rice bean, characterized the WRKY transcription factors, and studied their response to aluminum stress. …”
Get full text

Comparison Between Worker and Soldier Transcriptomes of Termite <i>Neotermes binovatus</i> Reveals Caste Specialization of Host–Flagellate Symbiotic System by Yu-Hao Huang, Miao Wang, Xiu-Ping Chang, Yun-Ling Ke, Zhi-Qiang Li

“…In this study, we sequenced high-depth transcriptomes from the workers and soldiers of a lower termite, <i>Neotermes binovatus</i> (Kalotermitidae), to investigate the differentially expressed termite transcripts, flagellate transcript abundance, and co-expression patterns of the host–flagellate transcript pairs in both castes. …”
Get full text

Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of <i>p</i>-Coumaric Acid on <i>Morchella importuna</i> by Qi Yin, Wenchang Zhang, Yingli Cai, Xiaofei Shi, Fuqiang Yu, Jianzhuang Guo, Xinhua He, Peixin He, Wei Liu

“…Therefore, we used physiological and comparative transcriptomic analyses to reveal its toxicity mechanism. …”
Get full text

Integrated Multi-Omics of the Longissimus Dorsal Muscle Transcriptomics and Metabolomics Reveals Intramuscular Fat Accumulation Mechanism with Diet Energy Differences in Yaks by Jingying Deng, Pengjia Bao, Ning Li, Siyuan Kong, Tong Wang, Minghao Zhang, Qinran Yu, Xinyu Cao, Jianlei Jia, Ping Yan

“…This study reveals the energy-dependent epigenetic regulatory mechanism of IMF deposition in plateau ruminants, which provides molecular targets for optimizing yak nutritional strategies and quality meat production, while having important theoretical and practical value for the sustainable development of livestock husbandry on the Tibetan Plateau.…”
Get full text

Integrated analysis of microbiome and host transcriptome revealed correlations between tissue microbiota and tumor progression in early-stage papillary thyroid carcinoma by Xiuwen Tong, Xiuwen Tong, Xipei Chen, Chen Shen, Jiahao Pan, Xinyu Wang, Xinyun Xu, Sheng Liu

“…However, the microbial distribution in the papillary thyroid carcinoma (PTC) tissue and its relationship with PTC are unclear.MethodsWe performed 16S rRNA amplicon sequencing and RNA-Seq to characterize the tissue microbiome and transcriptome between the tumor and paracancerous tissue, respectively. …”
Get full text

Comparative transcriptome analysis reveals abscisic acid-induced bHLH transcription factors involved in saikosaponin biosynthesis in Bupleurum chinense DC by Han Wang, Shanqun Hu, Tong Li, Xuejie Qu, Jiaqi Zhang, Baoshun Wang, Yixuan Sun, Rui Cao, Yutong Yan, Ze Song, Xia’nan Zhang, Rong Luo, Yuru Tong, Changli Liu

“…In this study, 20 differentially expressed BcbHLH genes were identified by transcriptomic profiling of ABA-induced hairy roots, with four MYC-family candidates (BcbHLH1-BcbHLH4) demonstrating ABA-responsive regulatory potential. …”
Get full text

Integrated analysis of single-cell RNA sequencing and bulk transcriptome data identifies a pyroptosis-associated diagnostic model for Parkinson’s disease by Lin Wang, Yidan Qin, Jia Song, Jing Xu, Wei Quan, Hang Su, Huibin Zeng, Jian Zhang, Jia Li, Jiajun Chen

“…This study established a pyroptosis-related diagnostic model for PD through the analyses of scRNA-seq combined with bulk transcriptome data, which improved the understanding of the role of PDEGs in PD and provided new insights into the diagnostic strategies for this neurodegenerative disease.…”
Get full text

Transcriptomic analysis of two Chinese wheat landraces with contrasting Fusarium head blight resistance reveals miRNA-mediated defense mechanisms by Lijuan Wu, Lijuan Wu, Junqiang Wang, Shian Shen, Zaijun Yang, Xinkun Hu

“…These insights into miRNA-mediated regulatory mechanisms provide a molecular basis for breeding FHB-resistant wheat varieties and highlight miRNA-mRNA interactions as promising targets for enhancing disease resilience.…”
Get full text

RNA-Seq Reveals Transcriptome Changes Following Zika Virus Infection in Fetal Brains in <i>c-Flip</i> Knockdown Mice by Ting Xie, Qiqi Chen, Nina Li, Shengze Zhang, Lin Zhu, Shaohui Bai, Haolu Zha, Weijian Tian, Chuming Luo, Nan Wu, Xuan Zou, Shisong Fang, Yuelong Shu, Jianhui Yuan, Ying Jiang, Huanle Luo

“…Furthermore, the impact of the c-FLIP deficiency on the transcriptome during a Zika virus (ZIKV) infection, which induces apoptosis and inflammation in the central nervous system (CNS), has not yet been elucidated. …”
Get full text

Axillary adipose tissue–derived lymphatic endothelial cells exhibit distinct transcriptomic signatures reflecting lymphatic invasion status in breast cancer by Asumi Iesato, Jun Suzuka, Kazutaka Otsuji, Tomo Osako, Nami Yamashita, Yuka Inoue, Tetsuyo Maeda, Natsue Uehiro, Kazuyo Yoshida, Yoko Takahashi, Kohei Kumegawa, Sumito Saeki, Liying Yang, Ai Tsuchiya, Kana Sakiyama, Miwa Tanaka, Takehiko Sakai, Shinji Ohno, Tetsuo Noda, Takayuki Ueno, Reo Maruyama

Get full text

Unveiling the Therapeutic Potential of Baicalin in Intervertebral Disc Degeneration: Integrative Bulk and Single-Cell Transcriptome Analysis with Experimental Validation of PANopto... by Wang X, Gao S, Zhou D, Cai W, Lv J, Wei Z, Song C, Shan X, Liu Z

“…Moreover, baicalin alleviated IL-1β-induced cell death in NPCs and improved histological features in the rat IVDD model.Conclusion: Our findings reveal a critical role of PANoptosis in IVDD progression and demonstrate that baicalin alleviates disc degeneration by inhibiting PANoptosis. This study provides novel insights into PANoptosis as a promising therapeutic target for IVDD.Keywords: intervertebral disc degeneration, Baicalin, PANoptosis, bioinformatics, single-cell…”
Get full text

Metabolome and transcriptome profiling reveals light-induced anthocyanin biosynthesis and anthocyanin-related key transcription factors in Yam (Dioscorea Alata L.) by Peipei Zhang, Shiqiang Xu, Long Zhang, Xulong Li, Jie Qi, Lin Weng, Shike Cai, Jihua Wang

“…Conclusions The results of this study provide useful insights into the regulation of light on anthocyanin accumulation in yam, and will be helpful for yam breeding and cultivation practices.…”
Get full text

Single-cell and spatial transcriptome analyses reveal MAZ(+) NPC-like clusters as key role contributing to glioma recurrence and drug resistance by Qiang Gu, Kan Wang, Tingming Lu, Yifei Xiao, Yaqing Wu, Hang Zhou, Kaijia Zhou

“…Advances in single-cell and spatial transcriptomics technologies provide feasible resolution for studying the cellular composition and dynamics of tumors. …”
Get full text

Transcriptome and Physiological Analysis Reveals the Mechanism of Abscisic Acid in Regulating Cadmium Uptake and Accumulation in the Hyperaccumulator <i>Phytolacca acinosa</i> Roxb... by Qin Xie, Wenting Xu, Qing Wang, Feihong Yao, Yachao Jiang, Haijia Cao, Wanhuang Lin

“…These results may help to elucidate the mechanisms by which ABA regulates Cd uptake and accumulation in plants, and provide a reference for developing a phytohormone-based strengthening strategy to improve the phytoremediation ability of other hyperaccumulators or accumulator species. …”
Get full text

Transcriptome Analysis Reveals Hyperglycemic Hormone and Excitatory Amino Acid Transporter 3 Are Involved in the Thermal Adaptation of <i>Eriocheir sinensis</i> by Xi Li, Runlin Zhou, Ruiqi Zhang, Zhen He, Mingzhi Zhang, Ran Li, Tong Hao, Jinsheng Sun

“…The results of this study not only provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in <i>E. sinensis</i>, but also establish a theoretical foundation for further research into thermal adaptation strategies in crustaceans.…”
Get full text

Comprehensive Stress-Based De Novo Transcriptome Assembly and Annotation of Guar (Cyamopsis tetragonoloba (L.) Taub.): An Important Industrial and Forage Crop by Fahad Al-Qurainy, Aref Alshameri, Abdel-Rhman Gaafar, Salim Khan, Mohammad Nadeem, Abdulhafed Abdullah Alameri, Mohamed Tarroum, Muhammad Ashraf

“…Therefore, whole mRNA sequence approach on the Guar plant was conducted to provide a snapshot of the mRNA information in the cell under salinity, heat, and drought stresses to be integrated with previous transcriptomic studies. …”
Get full text

A Cross-Tissue Transcriptome-Wide Association Study Reveals Novel Susceptibility Genes for Diabetic Kidney Disease in the FinnGen Cohort by Menghan Liu, Zehua Li, Yao Lu, Pingping Sun, Ying Chen, Li Yang

“…<b>Methods</b>: We performed a cross-tissue transcriptome-wide association study (TWAS) of DKD using expression quantitative trait loci (eQTL) data from 49 tissues in the Genotype—Tissue Expression (GTEx) version 8 (v8) resource. …”
Get full text

Transcriptomic analysis of rat prefrontal cortex following chronic stress induced by social isolation – Relevance to psychiatric and neurodevelopmental illness, and implications fo... by Jen-Yin Goh, Patricia Rueda, Joy Taylor, Alex Rathbone, Daniel Scott, Christopher J. Langmead, Kevin C.F. Fone, Gregory D. Stewart, Madeleine V. King

“…We used RNA sequencing (RNA-Seq) to enable unbiased assessment of transcriptomic changes within the prefrontal cortex (PFC) of isolation-reared rats. …”
Get full text

Transcriptomic and miRNA Signatures of ChAdOx1 nCoV-19 Vaccine Response Using Machine Learning by Jinting Lin, Qinglan Ma, Lei Chen, Wei Guo, Kaiyan Feng, Tao Huang, Yu-Dong Cai

“…These observations contribute to an understanding of the immune protection and inflammatory responses induced by the ChAdOx1 nCoV-19 vaccine during symptomatic episodes and provide a rationale for improving vaccine efficacy.…”
Get full text