Anthocyanin Degradation Drives Heat-Induced Petal Fading in <i>Chrysanthemum morifolium</i> at Full Bloom: A Multi-Omics Analysis by Ge Zhao, Yanan Li, Jialin Peng, Xiuge Li, Wenhao Xia, Yuhe Tian, Yukun Li, Lijie Zhou

“…In this study, ‘Nannong Fencui’ chrysanthemums at full bloom—when anthocyanin accumulation peaks—were exposed to 35 °C, while a control group was maintained at 22 °C, to assess heat stress effects on anthocyanin metabolism, including both biosynthesis and degradation. Transcriptomic analysis identified nine core structural genes and three key transcription factors involved in anthocyanin biosynthesis, along with twelve core genes linked to enzymatic anthocyanin degradation. …”
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Down-regulation of colon mucin production induced by Eimeria pragensis infection in mice by Yulia Dwi Setia, Yulia Dwi Setia, Mio Kokubo-Tanaka, Ryusei Tanaka, Akemi Yoshida, Eiji Nagayasu, Parnian Ahmadi, Parnian Ahmadi, Parnian Ahmadi, Ayako Yoshida, Ayako Yoshida, Haruhiko Maruyama

“…To elucidate mechanisms of goblet cell dysfunction, we performed RNA sequencing of large intestine tissue to examine host as well as parasite transcriptomes.ResultsFecal oocyst excretion peaked at 8–9 dpi. …”
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PRMT5 inhibition has a potent anti-tumor activity against adenoid cystic carcinoma of salivary glands by Vasudha Mishra, Alka Singh, Michael Korzinkin, Xiangying Cheng, Claudia Wing, Viktoria Sarkisova, Ashwin L. Koppayi, Alexandra Pogorelskaya, Oksana Glushchenko, Manu Sundaresan, Venkat Thodima, Jack Carter, Koichi Ito, Peggy Scherle, Anna Trzcinska, Ivan Ozerov, Everett E. Vokes, Grayson Cole, Frank W. Pun, Le Shen, Yuxuan Miao, Alexander T. Pearson, Mark W. Lingen, Bruce Ruggeri, Ari J. Rosenberg, Alex Zhavoronkov, Nishant Agrawal, Evgeny Izumchenko

“…Conclusion Taken together, our study underscores the role of PRMT5 in ACC oncogenesis and provides a strong rationale for the clinical development of PRMT5 inhibitors as a targeted monotherapy or combination therapy for treatment of patients with this rare disease, based on the analysis of their underlying molecular profile.…”
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Bacteroides uniformis-generated hexadecanedioic acid ameliorates metabolic-associated fatty liver disease by Da-Ya Zhang, Da Li, Shi-Ju Chen, Li-Jun Zhang, Xu-Li Zhu, Fa-Di Chen, Chen Chen, Qi Wang, Yiping Du, Jian-Xin Xiong, Shi-Mei Huang, Xiao-Dong Zhang, Yan-Ting Lv, Fan Zeng, Run-Xiang Chen, Xianfeng Huang, Fengjiao Mao, Shuo Zhou, Qicen Yao, Yuliang Huang, Runyu Chen, Ying Mo, Yunqian Xie, Yue-Hong Jiang, Zhai Chen, Cui-Yi Mo, Jia-Jia Chen, Fei-Hu Bai

“…B. uniformis and its potential putative metabolite HDA may contribute to MAFLD progression modulation, through regulation of the IRE1α-XBP1s axis. This study provides new insights into the gut-liver axis in MAFLD and offers promising therapeutic targets based on specific microbes and their metabolites.…”
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Genome-wide identification and functional characterization of LBD gene family in four Cymbidium species (Orchidaceae) and potential regulatory role of CsiLBD27 in floral developmen... by Yonglu Wei, Zengyu Lin, Jianpeng Jin, Wei Zhu, Jie Gao, Jie Li, Qi Xie, Chuqiao Lu, Genfa Zhu, Fengxi Yang

“…Conclusions Collectively, this study provides the first comprehensive insight into the evolutionary dynamics, regulatory mechanisms, and functional roles of LBD genes in Cymbidium. …”
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Distinct Patterns of Smooth Muscle Phenotypic Modulation in Thoracic and Abdominal Aortic Aneurysms by Chien-Jung Lin, Campbell Keating, Robyn Roth, Yasar Caliskan, Mustafa Nazzal, Vernat Exil, Richard DiPaolo, Divya Ratan Verma, Kishore Harjai, Mohamed Zayed, Chieh-Yu Lin, Robert P. Mecham, Ajay K. Jain

“…Single-cell RNA sequencing provides an opportunity to interrogate cell-type-specific transcriptomes. …”
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Chloroquine induces eryptosis in P. falciparum-infected red blood cells and the release of extracellular vesicles with a unique protein profile by Claudia Carrera-Bravo, Claudia Carrera-Bravo, Tianchi Zhou, Tianchi Zhou, Trang Chu, Jing Wen Hang, Harshvardhan Modh, Chenyuan Huang, Chenyuan Huang, Sitong Zhang, Sitong Zhang, Haining Hao, Haining Hao, Haining Hao, María José Cabada-García, Benoit Malleret, Benoit Malleret, Matthias G. Wacker, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Jiong-Wei Wang, Laurent Rénia, Kevin S. W. Tan

“…THP-1-derived macrophages were stimulated with EVs to determine parasite-host interactions, as indicated by cytokine levels and transcriptomic analysis.ResultsIncreased PS exposure, cell shrinkage, and membrane blebbing were observed, delineating an eryptotic phenotype in the host RBCs. …”
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Diabetes is causally associated with increased breast cancer mortality by inducing FIBCD1 to activate MCM5-mediated cell cycle arrest via modulating H3K27ac by Binbin Tan, Yang Liu, Qianqian Chen, Weijie Yang, Wenhan Yang, Kaiping Gao, Li Fu, Tiantian Zhang, Penglong Chen, Yongyi Huang, Yuting Wang, Guoqiang Zhang, Juan Xiong, Rihong Zhai

“…Mechanistically, hyperglycemia-activated FIBCD1 promoted MCM5 expression to induce S-phase cell cycle arrest by upregulating histone H3K27ac levels in MCM5 promoter via the PDH-acetyl-CoA axis. Our findings provide new evidence that co-existing DM has a causal effect on overall mortality in BC-DM patients. …”
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Addition of metformin to anti-PD-1/PD-L1 drugs activates anti-tumor immune response in peripheral immune cells of NSCLC patients by Luisa Amato, Caterina De Rosa, Gaetano Di Guida, Filippo Sepe, Annalisa Ariano, Sara Capaldo, Faiz Ul Haq, Alessandra Di Liello, Concetta Tuccillo, Stefano Lucà, Renato Franco, Viviana De Rosa, Francesca Iommelli, Alberto Servetto, Fortunato Ciardiello, Carminia Maria Della Corte, Floriana Morgillo

“…Patients with advanced-stage NSCLC provided peripheral blood mononuclear cells (PBMCs) for analysis. …”
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3D printing-biomimetic local stiff niche enhances glycolysis to boost PDAC cell stem-like phenotype via N6-methyladenosine-suppressed YAP1 mRNA decay by Di Wu, Xiaoqi Guan, Tao Yang, Jiashuai Yan, Biwen Zhu, Junchao Zhou, Yibing Guo, Yuhua Lu

“…Bioinformatics, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, of CSCs identified from spatial transcriptomic and single-cell datasets of PDAC lesions exhibited activated mechanical and glycolytic pathways. …”
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Anaerobic Faecalicatena spp. degrade sulfoquinovose via a bifurcated 6-deoxy-6-sulfofructose transketolase/transaldolase pathway to both C2- and C3-sulfonate intermediates by Sabrina Borusak, Sabrina Borusak, Sabrina Borusak, Karin Denger, Till Dorendorf, Till Dorendorf, Corentin Fournier, Corentin Fournier, Harry Lerner, Harry Lerner, Olga Mayans, Olga Mayans, Dieter Spiteller, Dieter Spiteller, David Schleheck, David Schleheck, David Schleheck

“…DSM22707 and Bilophila wadsworthia 3.1.6. Our findings provide the first description of an additional sulfoglycolytic, bifurcated SQ pathway. …”
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Genome-Wide Identification, Evolutionary Expansion, and Expression Analyses of Aux/IAA Gene Family in <i>Castanea mollissima</i> During Seed Kernel Development by Yujuan Tian, Jingmiao Huang, Jinxin Wang, Dongsheng Wang, Ruimin Huang, Xia Liu, Haie Zhang, Jingzheng Zhang, Xiangyu Wang, Liyang Yu

“…Furthermore, the reliability of the transcriptomic data was further confirmed by RT-qPCR experiments. …”
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Identification and validation of glycolysis-related diagnostic signatures in diabetic nephropathy: a study based on integrative machine learning and single-cell sequence by Xiaoyin Wu, Xiaoyin Wu, Buyu Guo, Buyu Guo, Xingyu Chang, Xingyu Chang, Yuxuan Yang, Yuxuan Yang, Qianqian Liu, Qianqian Liu, Jiahui Liu, Jiahui Liu, Yichen Yang, Yichen Yang, Kang Zhang, Yumei Ma, Songbo Fu, Songbo Fu, Songbo Fu

“…The current lack of reliable predictive biomarkers hinders early diagnosis and personalized therapy.MethodsTranscriptomic profiles of DN samples and controls were extracted from GEO databases. …”
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Genetic Dissection of the Powdery Mildew Resistance in a Cultivated Emmer Wheat Accession by Ruishan Liu, Yuli Jin, Ningning Yu, Hongxing Xu, Xusheng Sun, Jiangchun Wang, Xueqing Liu, Jiadong Zhang, Jiatong Li, Yaoxue Li, Pengtao Ma

“…The flanking markers could accurately trace the presence of <i>PmL709</i> from resistant accession L709 in a survey of 46 susceptible wheat accessions. These findings provide valuable insights into the genetic and molecular mechanisms of powdery mildew resistance in wheat and offer practical tools for marker-assisted breeding to develop resistant cultivars.…”
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SNP rs615552 and lncRNA CDKN2B-AS1 influence brain cancer pathogenesis through multi-omic mechanisms by Zheng Ye, Jiaqi Yuan, Qianbei Yi, Peng Xu, Wenbin Liu

“…Integration with GWAS-GWAS colocalization analysis provided insights into shared genetic mechanisms with other diseases. …”
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Differential Mitochondrial Genome Expression of Four Skink Species Under High-Temperature Stress and Selection Pressure Analyses in Scincidae by Xuxiang Wu, Lemei Zhan, Kenneth B. Storey, Jiayong Zhang, Danna Yu

“…To validate these findings, transcriptomic profiling was conducted to assess mitochondrial protein-coding gene (PCG) expression patterns under thermal stress. …”
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ALDOC promotes neuroblastoma progression and modulates sensitivity to chemotherapy drugs by enhancing aerobic glycolysis by Yunpeng Chen, Haixia Zhu, Yishu Luo, Tianyue Xie, Youyang Hu, Zhiwei Yan, Weichao Ji, YaXuan Wang, Qiyou Yin, Hua Xian

“…This study investigates the role of the glycolysis-associated gene ALDOC in NB pathogenesis and its impact on chemotherapy sensitivity.MethodsTranscriptomic data from NB patients were analyzed to identify ALDOC as an independent risk factor for high-risk NB. …”
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Genomic subtypes of non-muscle-invasive bladder cancer: guiding immunotherapy decision-making for patients exposed to aristolochic acid by Yun Peng, Yuxuan Song, Caipeng Qin, Mengting Ding, Zixiong Huang, Fei Wang, Yuchao HuangFu, Luping Yu, Yiqing Du, Tao Xu

“…Conclusions Our findings provide novel perspectives on the genomics of NMIBC, unveiling four prominent genomic subtypes, each showing different outcomes following immunotherapy. …”
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The role of endothelial cell-related gene COL1A1 in prostate cancer diagnosis and immunotherapy: insights from machine learning and single-cell analysis by Gujun Cong, Jingjing Shao, Feng Xiao, Haixia Zhu, Peipei Kang

“…Method By integrating clinical and transcriptomic data from TCGA-PRAD, we initially identified key endothelial cell-related genes in PRAD samples through single-cell analysis. …”
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Empagliflozin-Pretreated MSC-Derived Exosomes Enhance Angiogenesis and Wound Healing via PTEN/AKT/VEGF Pathway by Wang H, Bai Z, Qiu Y, Kou J, Zhu Y, Tan Q, Chen C, Mo R

“…This strategy offers a promising approach for improving diabetic wound repair and provides a potential new therapeutic avenue in regenerative medicine. …”
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