Time-restricted feeding protects against septic liver injury by reshaping gut microbiota and metabolite 3-hydroxybutyrate by Jing-Juan Hu, Fan Deng, Qi-Shun Sun, Qing-Ming Xiong, Yue Min, Si-Yuan Feng, Ze-Bin Lin, Peng-Han Chen, Zhen Hu, Ling Wu, Xiao-Feng Chen, Sun Xie, Wei-Feng Liu, Cai Li, Ke-Xuan Liu

“…Overall, this study elucidates the protective mechanism of TRF against septic liver injury and identifies 3-HB as a potential therapeutic target and predictive biomarker, thereby providing new insights into the clinical management and diagnosis of septic liver injury.…”
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IL-6 predicts CDK4/6 inhibitor resistance, identifying STAT3 as a target in HR + /HER2-negative metastatic breast cancer by Nicole M. Kettner, Tuyen N. Bui, Juliana Navarro-Yepes, Sanaz Ghotbaldini, Bethanie Quintela, Catherine K. Luo, Nghi Lam, Xiayu Rao, Akshara Singareeka Raghavendra, Yan Wang, Nancy Azizian, T. Kris Eckols, Moses Makokha Kasembeli, Kurt Evans, Min Yi, Hannah Wingate, Jing Wang, Aysegul A. Sahin, Funda Meric-Bernstam, Kelly K. Hunt, Senthil Damodaran, David J. Tweardy, Debu Tripathy, Khandan Keyomarsi

“…Treatment with TTI-101, a STAT3 inhibitor, significantly reduced tumor growth and improved survival in these models, providing preclinical validation for targeting the IL-6/STAT3 axis. …”
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An atlas of neuropathic pain-associated molecular pathological characteristics in the mouse spinal cord by Fu-Lu Dong, Lina Yu, Pei-Da Feng, Jin-Xuan Ren, Xue-Hui Bai, Jia-Qi Lin, De-Li Cao, Yu-Tao Deng, Yan Zhang, Hui-Hui Shen, Hao Gong, Wen-Xing Sun, Dong-Qiu Chi, Yixiao Mei, Longfei Ma, Ming-Zhe Yin, Meng-Na Li, Peng-Fei Zhang, Nan Hu, Bing-Lin Zhou, Ying Liu, Xuan-Jie Zheng, Yi-Fan Chen, Da Zhong, Yuan-Xiang Tao, Min Yan, Bao-Chun Jiang

“…We here integrate spatial transcriptomics (ST) with single-nucleus RNA-sequencing (snRNA-seq) and bulk RNA-sequencing (bulk RNA-seq) to characterize regional pathological heterogeneity of the SC under NP conditions. …”
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Screened of long non-coding RNA related to wool development and fineness in Gansu alpine fine-wool sheep by Zhaohua He, Fangfang Zhao, Hongxian Sun, Jiang Hu, Jiqing Wang, Xiu Liu, Mingna Li, Zhiyun Hao, Zhidong Zhao, Bingang Shi, Feiyan Liu, Shaobin Li

“…In conclusion, the research enlarges the current lncRNAs database, providing a new insight for the investigation of wool fineness development in fine-wool sheep.…”
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An anti-androgen resistance-related gene signature acts as a prognostic marker and increases enzalutamide efficacy via PLK1 inhibition in prostate cancer by Ruilin Zhuang, Ruihui Xie, Shirong Peng, Qianghua Zhou, Weilong Lin, Yuan Ou, Bingliang Chen, Tong Su, Zean Li, Hai Huang, Kaiwen Li, Yu Duan

“…The integration of this signature with immune profiling and drug sensitivity analysis provides a valuable tool for precision oncology in PCa. …”
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Human proximal tubular epithelial cell interleukin-1 receptor signalling triggers G2/M arrest and cellular senescence during hypoxic kidney injury by Kurt T. K. Giuliani, Purba Nag, Benjamin C. Adams, Xiangju Wang, Seokchan Hong, Anca Grivei, Rebecca L. Johnston, Nicola Waddell, Kenneth K. C. Ho, Yilin Tian, Muhammad Ali Khan, Chang Seong Kim, Monica S. Y. Ng, Glenda Gobe, Jacobus P. J. Ungerer, Josephine M. Forbes, Helen G. Healy, Andrew J. Kassianos

“…Hypoxic PTECs incubated with IL-1β displayed a discrete transcriptomic profile distinct from PTECs cultured under hypoxia alone, IL-1β alone or under normoxia. …”
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Inonotus obliquus (chaga) ameliorates folic acid-induced renal fibrosis in mice: the crosstalk analysis among PT cells, macrophages and T cells based on single-cell sequencing by Yueling Peng, Yaling Zhang, Yaling Zhang, Rui Wang, Xinyu Wang, Xingwei Liu, Hui Liao, Rongshan Li

“…Cell-to-cell communication analyses revealed potential interactions between proximal tubular cells, macrophages, and T Cells, providing insights into possible mechanisms by which Chaga may ameliorate renal fibrosis.ConclusionOur study provided new insights into the potential therapeutic effects of Chaga in renal fibrosis through single-cell sequencing analysis. …”
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Methyl 2-methylbutyrate produced by Streptomyces saccharicans strain ZBY1-20 inhibits Monilinia fructicola causing peach brown rot by Shan Chen, Meijun Chen, Yang Zheng, Rong Mu, Haorong Yang, Mengfen Liu, Jihong Jiang, Guanhua Ma, Xianchao Sun, Kuo Huang, Jiequn Ren, Guoju Dong, Guokang Chen

“…Overall, these findings highlight the efficacy of S. saccharicans strain ZBY1-20 in managing peach brown rot via the production of methyl 2-methylbutyrate, providing an environmentally friendly alternative for postharvest disease control and advancing strategies for fruit preservation.…”
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Attenuated immune surveillance during squamous cell transformation of pancreatic adenosquamous cancer defines new therapeutic opportunity for cancer interception by Jie Chen, Xianlong Chen, Xinyuan Chen, Shanyue Sun, Shuofeng Li, Shuangni Yu

“…These findings were validated in an independent cohort study.Conclusion Our study advances the understanding of PASC by providing updated insights into its immunoenvironmental features. …”
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Chaihuang Qingfu Pills Protect Against Acute Pancreatitis—Associated Acute Lung Injury Through MMP9-NLRP3-Pyroptosis Pathway by Xiao W, Shi H, Tian Y, Chen F, Xie Y, Han X, Zhang X, Cao Y, Liu W, Zhu Y, Liu Y, Jiang Y

“…UHPLC-MS/MS was used to identify the components of CHQF absorbed into the serum. Transcriptomics analysis, network pharmacology, and proteomics approaches were used to explore the underlying molecular mechanism. …”
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Comprehensive analysis of multi‐omics single‐cell data using the single‐cell analyst by Lu Pan, Bufu Tang, Xuan Zhang, Paolo Parini, Roman Tremmel, Joseph Loscalzo, Volker M. Lauschke, Bradley A. Maron, Paola Paci, Ingemar Ernberg, Nguan Soon Tan, Ákos Végvári, Zehuan Liao, Sundararaman Rengarajan, Roman Zubarev, Yuxuan Fan, Xu Zheng, Xinyue Jian, Ren Sheng, Zhenning Wang, Xuexin Li

“…The platform offers both online and offline modes, providing flexibility for various use cases. It automates critical analysis steps, such as quality control, data processing, and phenotype‐specific analyses, while also offering interactive, publication‐ready visualizations. …”
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Revealing the core suppression effects of various Di (2-ethylhexyl) phthalate exposure on early meiosis progression in postnatal male mice via single-cell RNA sequencing by Baoquan Han, Lei Hua, Shuai Yu, Wei Ge, Cong Huang, Yu Tian, Chunxiao Li, Jiamao Yan, Tian Qiao, Jiachen Guo, Dongliang Lu, Bin Wang, Diya Cai, Yunqi Zhang, Shaolin Liang, Jianjuan Zhao, Qi Hou, Wei Shen, Zhongyi Sun

“…This study offers the first comprehensive evidence that DEHP inhibits early meiotic progression in male germ cells through the Trp53-mediated p38-MAPK pathway, providing crucial insights into the molecular mechanisms underlying DEHP-induced male reproductive toxicity. …”
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Integrated lncRNA and mRNA analysis reveals the immune modulatory mechanisms of antimicrobial peptide BSN-37 in mouse peritoneal macrophages by Huihui Zhang, Yanhe Lv, Jingjing Li, Bingze Jiao, Jiahui Fu, Xujie Zhao, Likun Cheng, Yilin Bai, Lei Wang, Yanwei Li, Bolin Hang, Xiaobing Wei, Mingcheng Liu, Zhanwei Teng, Meinan Chang, Chengshui Liao, Yueyu Bai, Xiaojing Xia, Ke Ding, Jianhe Hu

“…This study aimed to elucidate the key targets and pathways through which the antimicrobial peptide BSN-37 modulates immune responses in macrophages, providing evidence for its potential clinical translation. …”
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Multi-omics landscape of alternative splicing in diffuse midline glioma reveals immune- and neural-driven subtypes with implications for spliceosome-targeted therapy by Chaxian Liu, Chaxian Liu, Chaxian Liu, Chaxian Liu, Chaxian Liu, Yue Liu, Yue Liu, Yue Liu, Yue Liu, Yue Liu, Hao Lin, Hao Lin, Hao Lin, Hao Lin, Hao Lin, Chufan Zhang, Chufan Zhang, Chufan Zhang, Bilong Zhang, Bilong Zhang, Bilong Zhang, Haikun Song, Haikun Song, Haikun Song, Xiaomin Fan, Yi Lyu, Hui Yang, Hui Yang, Hui Yang, Hui Yang, Hui Yang, Ying Mao, Ying Mao, Ying Mao, Ying Mao

“…Given its potential impact on gene regulation and tumor progression, a comprehensive analysis of alternative splicing could provide novel insights into targeted or immune therapeutic strategies, complementing existing transcriptomic studies of DMG.MethodsTo investigate the alternative splicing landscape of DMG, we performed transcriptome sequencing (RNA-seq) on patient-derived H3WT and H3K27-altered DMG cell lines, integrating these data with RNA-seq and single-cell transcriptomic (scRNA-seq) datasets from published sources. …”
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In-depth functional analysis of BRD9 in fetal hematopoiesis reveals context-dependent roles by Yifan Zhang, Masaki Nomura, Koutarou Nishimura, Weijia Zang, Yui Koike, Muran Xiao, Hiromi Ito, Miki Fukumoto, Atsushi Tanaka, Yumi Aoyama, Wataru Saika, Chihiro Hasegawa, Hiromi Yamazaki, Akifumi Takaori-Kondo, Daichi Inoue

“…Through comprehensive transcriptomic and epigenomic analysis, we elucidate the differential roles of BRD9 in a context- and lineage-dependent manner. …”
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The antimicrobial peptide Cec4 has therapeutic potential against clinical carbapenem-resistant Klebsiella pneumoniae by Lu Li, Yang Zeng, Minfang Tian, Huijun Cao, Zhilang Qiu, Guo Guo, Feng Shen, Yuping Wang, Jian Peng

“…Moreover, in vivo studies using a mouse skin wound model confirmed the efficacy of Cec4, and transcriptomic analysis shed light on the molecular mechanisms underlying its antibacterial activity. …”
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Exploring the biological basis for the identification of different syndromes in ischemic heart failure based on joint multi-omics analysis by Yilin Zhang, Yilin Zhang, Jingjing Wei, Lijie Qiao, Rui Yu, Hongjie Ren, Anshe Zhao, Yang Sun, Aolong Wang, Aolong Wang, Bin Li, Xinlu Wang, Mingjun Zhu

“…External validation demonstrated the above core targets.ConclusionThis study investigated the biological basis of QDBS, YDBS and YDBSFR in IHF from a modern biomedical perspective, providing references for the objective research of TCM syndrome differentiation.…”
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miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken by Qingqing Geng, Cai Hu, Ziduo Zhao, Zhe Wang, Fufu Cheng, Jing Chen, Qisheng Zuo, Yani Zhang

“…In this study, miR-1458, which was differentially expressed during SSCs formation, was selected by transcriptomic sequencing. We found that miR-1458, inhibited in an in vitro SSCs induction model,significantly upregulated the expression of germline marker genes (Cvh and integrin β1). …”
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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

“…We describe efficient methods for the isolation and growth of NCMs and report their phenotypic, proteomic, and transcriptomic characteristics, which will facilitate the investigation of UM development and progression. …”
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Multimodal fusion of radio-pathology and proteogenomics identify integrated glioma subtypes with prognostic and therapeutic opportunities by Zaoqu Liu, Yushuai Wu, Hui Xu, Minkai Wang, Siyuan Weng, Dongling Pei, Shuang Chen, WeiWei Wang, Jing Yan, Li Cui, Jingxian Duan, Yuanshen Zhao, Zilong Wang, Zeyu Ma, Ran Li, Wenchao Duan, Yuning Qiu, Dingyuan Su, Sen Li, Haoran Liu, Wenyuan Li, Caoyuan Ma, Miaomiao Yu, Yinhui Yu, Te Chen, Jing Fu, YingWei Zhen, Bin Yu, Yuchen Ji, Hairong Zheng, Dong Liang, Xianzhi Liu, Dongming Yan, Xinwei Han, Fubing Wang, Zhi-Cheng Li, Zhenyu Zhang

“…Additionally, we develop a deep neural network (DNN) classifier based on radiological features to further enhance the clinical translatability, providing a non-invasive tool for predicting MOFS subtypes. …”
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