Inhibition of Colorectal Cancer by Perillaldehyde Through Targeting SRD5A1 to Induce Autophagy via the PI3K/AKT Pathway by Leng Z, Zhang Y, Guo M, Chen Y, Wang Z

“…In vivo, a subcutaneous xenograft mouse model was established to evaluate the anti-CRC efficacy of PAH. Transcriptomic analysis was performed to identify possible mechanisms, particularly related to autophagy, and validated through TEM, immunofluorescence, Western blotting, and inhibitor assays. …”
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Bacterial Mediation of Environmental Impacts: Unveiling novel pathways for mitigation and stewardship by Seilkhan Ainur, Raiymbekova Indira, Zhunissov Abaibek, Zhamanbayeva Manira Krykbaevna, Bukunova Almira Shoshanovna, Toktaganova Gulzhas

“…By climate change as a defining challenge of our era, our research pioneers the investigation of microbial communities' responses to environmental stressors. Through meta-transcriptomic analysis, we uncover unique microbial strategies for carbon sequestration and adaptation, providing a blueprint for enhancing ecosystem resilience in a changing climate. …”
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Establishment of a Novel in vitro Model of Sepsis-Induced Myocardial Injury Using Septic Serum: A Comprehensive Comparative Study by Yang H, Feng L, Jiang Z, Deng R, Wu X, Zeng K

“…In contrast, cardiomyocytes exposed to septic serum demonstrated more pronounced inflammatory responses, oxidative stress, apoptosis, and myocardial damage. Transcriptomic analysis revealed that the septic serum model induced 706 DEGs, significantly more than LPS (262 DEGs) or TNF-α (237 DEGs), and enriched in a broader array of biological processes and signaling pathways. …”
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Blood TCTP as a potential biomarker associated with immunosuppressive features and poor clinical outcomes in metastatic gastric cancer by Changhoon Yoo, Hyung-Don Kim, Min-Hee Ryu, Yeong Hak Bang, Jiae Kim, Won-Tae Kim, Hayoung Lee, Hideyuki Yanai, Gwanghee Lee, Hee Jeong Kim, Seyoung Jung, Hyung Eun Lee, Jaewon Hyung, Myeong-Jin Yoon, Jeong-Hyun Ryou, Hyungsu Jeon, Jeong Seok Lee

“…Spatial transcriptomics analyses revealed a tumor-cell-enriched cluster co-localized with the Macrophage-chemokine subset, which exhibited the highest TPT1 expression and a positive correlation between its abundance and average TPT1 levels. …”
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Diurnal nectar secretion dynamics in Roscoea cautleoides (Zingiberaceae) reveal the role of non-sugar chemicals in plant–pollinator interaction by Wen-Jing Wang, Ya-Ting Lin, Hong-Fan Chen, Mei-Yuan Huang, Zong-Xin Ren, Jian-Li Zhao

“…By integrating metabolomics and transcriptomics, we explored the daily variations in nectar chemical composition and the gene expression in nectaries, with a specific focus on nectar metabolites from bagged flowers. …”
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Berberine alters the gut microbiota metabolism and impairs spermatogenesis by Qu Wei, Xu Yumin, Yang Jing, Shi Hanqing, Wang Junli, Yu Xinnai, Chen Jiemin, Wang Binyi, Zhuoga Deqing, Luo Mengcheng, Liu Rong

“…Overall, this study reveals an association between a BBR-induced decrease in Muribaculaceae abundance and defective spermatogenesis, providing a prospective therapeutic approach for addressing infertility-related decreases in serum testosterone triggered by changes in the gut microbiota composition.…”
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Anti-inflammatory therapy for tendinopathy using Il1rn mRNA encapsulated in SM102 lipid nanoparticles by Yuan Zhang, Yuan Zhang, Xu Li, Hao Li, Ruiyang Zhang, Ti Zhang, Talante Juma, Yongfei Zhou, Quanyi Guo, Hui Zhao, Yongping Cao

“…This SM102-Il1rn mRNA therapy enables spatiotemporally controlled anti-inflammatory therapy, providing a promising non-surgical solution for refractory tendinopathies. …”
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In-vitro immunomodulatory efficacy of extracellular vesicles derived from TGF-β1/IFN-γ dual licensed human bone marrow mesenchymal stromal cells by Jiemin Wang, Seyedmohammad Moosavizadeh, Manon Jammes, Abbas Tabasi, Trung Bach, Aideen E. Ryan, Thomas Ritter

“…In addition, publicly available transcriptomic datasets (GSE122091 and GSE46019) were analyzed to identify differentially expressed genes (DEGs) in IFN-γ– and TGF-β1–licensed MSCs, providing insight into potential molecular drivers of EV-mediated immunoregulation. …”
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Molecular and pharmacological heterogeneity of ETV6::RUNX1 acute lymphoblastic leukemia by Zhenhua Li, Huanbin Zhao, Wenjian Yang, Maud Maillard, Satoshi Yoshimura, Yu-Chih Hsiao, Xin Huang, Yoshihiro Gocho, Lauren Rowland, Anthony Brown, Landon Choi, Kristine R. Crews, Charles G. Mullighan, Samuel W. Brady, Cheng Cheng, Ti-Cheng Chang, Gang Wu, Mignon L. Loh, Allen Eng Juh Yeoh, Federico Antillon-Klussmann, Sima Jeha, Hiroto Inaba, Jiyang Yu, Ching-Hon Pui, Seth E. Karol, William E. Evans, Jun J. Yang

“…Our results point to molecular heterogeneity within ETV6::RUNX1 ALL linked to divergent drug responses, providing insights into the pathogenesis and therapeutic vulnerability of this common pediatric ALL subtype.…”
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4D Biofabrication of Magnetically Augmented Callus Assembloid Implants Enables Rapid Endochondral Ossification via Activation of Mechanosensitive Pathways by Konstantinos Ioannidis, Andreas Dimopoulos, Isaak Decoene, Maya Guilliams, Hanna Svitina, Liudmyla Storozhuk, Rodrigo de Oliveira‐Silva, Sergey Basov, Nguyen Thi Kim Thanh, Stefanos Mourdikoudis, Margriet J. Van Bael, Bart Smeets, Dimitrios Sakellariou, Ioannis Papantoniou

“…The integration of magnetic nanoparticles (MNPs) with cartilaginous microtissues enables model‐driven 4D bottom‐up biofabrication of remotely actuated assembloids, providing unique properties to mechanoresponsive tissues, particularly skeletal constructs. …”
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Machine learning identifies genes linked to neurological disorders induced by equine encephalitis viruses, traumatic brain injuries, and organophosphorus nerve agents by Liduo Yin, Morgen VanderGiessen, Morgen VanderGiessen, Vinoth Kumar, Benjamin Conacher, Po-Chien Haku Chao, Michelle Theus, Erik Johnson, Kylene Kehn-Hall, Kylene Kehn-Hall, Xiaowei Wu, Hehuang Xie

“…By examining combined gene expression profiles, common and unique molecular characteristics may be discovered, providing new insights into medical countermeasures (MCMs) for TBI, EEV infection and OPNA neuropathologies and sequelae. …”
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Whole-Genome Resequencing Analysis of Copy Number Variations Associated with Athletic Performance in Grassland-Thoroughbred by Wenqi Ding, Wendian Gong, Tugeqin Bou, Lin Shi, Yanan Lin, Xiaoyuan Shi, Zheng Li, Huize Wu, Manglai Dugarjaviin, Dongyi Bai

“…This study aims to address the lack of research on the impact of copy number variation (CNV) on racing performance in horse populations, providing new insights for locally bred racing breeds. …”
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Sensitivity to immune checkpoint inhibitors in BRAF/MEK inhibitor refractory melanoma by Grant A McArthur, Reem Saleh, Lorey Smith, Karen E Sheppard, Riyaben P Patel, Lydia Rui Jia Lim, Darius Schenk, Michael K Lee, Emily Lelliott, Aparna D Rao, Shaghayegh Arabi, Anna S Trigos, Nicole Haynes

“…RNA sequencing was performed to profile transcriptomic changes in resistant tumors, providing insights into the signaling pathways associated with resistance. …”
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Genome-wide identification and expression analysis of growth-regulating factor (GRF) and GRF-interacting factor (GIF) gene families in Castanea mollissima by WANG Xiangyu, TIAN Yujuan, LI Yun, WANG Dongsheng, WANG Xuan, GUO Chunlei, LIU Jing, ZHANG Hai’e, ZHANG Jingzheng, YU Liyang

“…In this study, the Chinese chestnut GRF and GIF gene families were studied to provide a theoretical basis for analyzing the potential functions of GRF and GIF genes in Chinese chestnut development and response to environmental stresses. …”
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Genome streamlining of Pseudomonas putida B6-2 for bioremediation by Siqing Fan, Hao Ren, Xueni Fu, Xiangyu Kong, Hao Wu, Zhenmei Lu

“…Targeted genome streamlining is an effective way to provide powerful chassis cells. However, genome streamlining does not always lead to the improved phenotypes of genome-streamlined chassis cells. …”
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Genome‐wide prediction of synthetic rescue mediators of resistance to targeted and immunotherapy by Avinash Das Sahu, Joo S Lee, Zhiyong Wang, Gao Zhang, Ramiro Iglesias‐Bartolome, Tian Tian, Zhi Wei, Benchun Miao, Nishanth Ulhas Nair, Olga Ponomarova, Adam A Friedman, Arnaud Amzallag, Tabea Moll, Gyulnara Kasumova, Patricia Greninger, Regina K Egan, Leah J Damon, Dennie T Frederick, Livnat Jerby‐Arnon, Allon Wagner, Kuoyuan Cheng, Seung Gu Park, Welles Robinson, Kevin Gardner, Genevieve Boland, Sridhar Hannenhalli, Meenhard Herlyn, Cyril Benes, Keith Flaherty, Ji Luo, J Silvio Gutkind, Eytan Ruppin

“…Here, we perform a genome‐wide in silico prediction of SR rescuer genes by analyzing tumor transcriptomics and survival data of 10,000 TCGA cancer patients. …”
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Tumor microenvironment assessment-based signatures for predicting response to immunotherapy in non-small cell lung cancer by Jiani Wu, Yuanyuan Wang, Zhenhua Huang, Jingjing Wu, Huiying Sun, Rui Zhou, Wenjun Qiu, Zilan Ye, Yiran Fang, Xiatong Huang, Jianhua Wu, Jianping Bin, Yulin Liao, Min Shi, Jiguang Wang, Wangjun Liao, Dongqiang Zeng

“…The IKCscore exhibits promise as a bioindicator that can predict the efficacy of both immunotherapy and immunotherapy-based combination therapies, while providing guidance for personalized therapeutic strategies for advanced NSCLC patients.…”
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Host taxonomy and environment shapes insectivore viromes and viral spillover risks in Southwestern China by Ji-Hu Yang, Chun-Feng Luo, Rong Xiang, Jiu-Meng Min, Zong-Ti Shao, Yi-Lin Zhao, Lu Chen, Lin Huang, Yun Zhang, Shun-Shuai Liu, Yu-Qiong Li, En-Nian Pu, Wen-Qiang Shi, Hai-Feng Pan, Wei-Jun Chen, Chun-Hong Du, Jia-Fu Jiang

“…Results This study employed a meta-transcriptomic approach to profile the viromes of 214 individual insectivores, encompassing 13 species from the families Soricidae, Erinaceidae, and Talpidae, collected across 12 counties in Yunnan Province, a recognized zoonotic hotspot. …”
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Immune and vascular modulation by HERVs: the role of CXCR1 and IL18RAP in dengue severity progression by Cristina Santos Ferreira, Alan Tardin Da Silva, Otávio José Bernandes Brustolini, Beatriz Rodrigues Pellegrina Soares, Beatriz Rodrigues Pellegrina Soares, Erika Regina Manuli, Erika Regina Manuli, Erika Regina Manuli, Mariana Severo Ramundo, Glaucia Paranhos-Baccala, Ester Cerdeira Sabino, Ester Cerdeira Sabino, Ana Tereza Ribeiro Vasconcelos

“…Dengue fever ranges from mild symptoms to severe cases characterized by plasma leakage and immune dysregulation, providing a relevant context to investigate these interactions.MethodsThis study comes up with a comprehensive analysis of differentially expressed HERVs (DE-HERVs), protein-coding genes (DEGs), and regulatory elements such as microRNAs (DE-miRNA) and non-LTR retroviruses (DE-LINEs and DE-SINEs) derived from the transcriptomes of Brazilian dengue patients across different disease stages.ResultsThe results show that DE-HERVs are associated with key genes identified in severe dengue cases, including ARG1, SLC15A2, COL3A1, SVEP1, CH25H, CST7, CXCR1, IL18RAP, SORL1, and TACR1, suggesting their role in immune modulation and endothelial permeability. …”
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Retinoic acid modulates peritoneal macrophage function and distribution to enhance antibacterial defense during inflammation by Yujuan Qin, Yujuan Qin, Xi Wang, Xi Wang, Xiamin Zhang, Xiamin Zhang, Lianting Nong, Lianting Nong, Qiyan Hou, Yuhong Chen, Yuhong Chen, Yuting Li, Yuting Li, Wenxian Lin, Xiuli Mao, Kezhao Wu, Wenqian Nong, Tonghua Wang, Lingzhang Meng, Lingzhang Meng, Jian Song, Jian Song, Jian Song

“…The effects of RA on macrophage phagocytic capacity, population dynamics, and transcriptomic profiles were assessed using immunofluorescence, flow cytometry, RNA sequencing, and quantitative PCR. …”
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