FAT1 mutation-related signature predicts survival risk and tumor immunogenicity in lung adenocarcinoma by Lifeng Gao, Xueying Wang, Yixin Xu, Aimin Wang, Wenjing Zhang, Qinghua Wang, Yanfeng Ren

“…In this study, we aim to identify a FAT1 mutation-related transcriptomic risk signature to assess the survival risks and immune status of LUAD patients.MethodsA total of 2528 LUAD samples, which included both gene expression profiles and clinicopathologic data, were collected from 12 datasets. …”
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Interactive effects of hydrodynamics and microplastics on bioaccumulation, histopathological alterations, biomarker responses, and gene expression in grass carp brain by Majid Rasta, Mian Adnan Kakakhel, Mojtaba S. Taleshi, Niloofar S. Lashkaryan, Jia Manke, Liming Liu, Shan-e-hyder Soomro, Xiaotao Shi

“…Transcriptomic analysis showed a significant variation in gene expression with associated key pathways such as DNA repair, RNA transport, FoxO signaling, and MAPK signaling, indicating active cellular responses to genetic damage. …”
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Robust HPV‐16 Detection Workflow for Formalin‐Fixed Cancer Tissue and Its Application for Oral Squamous Cell Carcinoma by Shizuka Morodomi, Akiyuki Hirosue, Akhinur Rahman, Kyotaro Nohata, Misaki Matsuo, Omnia Reda, Samiul Alam Rajib, Haruki Saito, Hiroki Takeda, Ryoji Yoshida, Masafumi Nakamoto, Masatoshi Hirayama, Kenta Kawahara, Mitsuyoshi Takatori, Yorihisa Orita, Hideki Nakayama, Yorifumi Satou

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Targeting metzincins to mitigate jellyfish blooms: a novel approach for conservation by Xuecun Liu, Xuecun Liu, Xiao Peng, Jingqiang Wang, Shuhui Ju, Qing Sun, Wensai Ji, Xiaoyu Hua, Haiyan Zhang, Juan Höfer, Marina Pozzolini, Shuogui Xu, Xueqi Ma, Liang Xiao

“…Through conducting the detection of metzincin proteolytic activity, molecular docking and kinetic analysis, as well as transcriptomic analysis and RT-qPCR verification before and after EDTA treatment of the polyp, the key biological toxins and the mechanisms influencing polyp budding were clarified.ResultsFour types of the metzincin family of metalloproteinases constituted the main biotoxins in the A. coerulea polyp. …”
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Chalcone isomerase gene (OsCHI3) increases rice drought tolerance by scavenging ROS via flavonoid and ABA metabolic pathways by Ting Liu, Ling Liu, Tianshun Zhou, Yinke Chen, Huang Zhou, Jiahan Lyu, Di Zhang, Xiwen Shi, Dingyang Yuan, Nenghui Ye, Meijuan Duan

“…These findings demonstrate that OsCHI3 increases drought stress tolerance in rice by activating the antioxidant defense system and the ABA metabolic pathway, providing new clues for drought-resistant rice breeding research.…”
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Microbial and molecular signatures for enhanced gut health in pigs fed a diet with spray-dried plasma as functional protein source by Soumya K. Kar, Dirkjan Schokker, Carine van Vuure, Herman van Iersel, Amy C. Harms, Amy C. Harms, Leo Kruijt, Mari A. Smits, Mari A. Smits, Alfons J. M. Jansman

“…These metabolites act as distinctive “fingerprints,” contributing to gut health by providing cellular energy, restoring tight junctions, and orchestrating immune responses in the small intestine mucosa. …”
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Tie2-mediated CBL ubiquitination of EGFR underlies ultrasound-responsive silk fibroin/graphene oxide hydrogel-troxerutin therapy for intervertebral disc degeneration by Youfeng Guo, Xiao Liu, Chao Wang, Shuguang Wang, Yufeng Huang, Shuo Ding, Zeyu Wang, Feng Wang

“…The SF/GO-gel platform facilitated the localized, ultrasound-triggered release of Trox, providing a novel approach to IDD therapy that targets the Tie2/EGFR axis. …”
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Linking a polyketide synthase gene cluster to 6-pentyl-alpha-pyrone, a Trichoderma metabolite with diverse bioactivities by Daniel Flatschacher, Alexander Eschlböck, Siebe Pierson, Ulrike Schreiner, Valentina Stock, Arne Schiller, David Ruso, Maria Doppler, Veronika Ruzsanyi, Mario Gründlinger, Christoph Büschl, Rainer Schuhmacher, Susanne Zeilinger

“…Conclusion Our findings establish pks1 as an essential gene for 6-PP biosynthesis in T. atroviride, providing novel insights into the production of one of the most significant compounds of this mycoparasite. …”
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Gut microbiota regulates innate anxiety through neural activity of medial prefrontal cortex in male mice by Jing Ren, Xiao-Ying Lian, Wan-Qian Ye, You-Lu Wen, Cheng-Lin Lu, Xiong Cao

“…Fiber photometry confirmed heightened mPFC neuronal activity during innate anxiety states in HA-FMT mice.DiscussionOur findings establish that gut microbiota modulates innate anxiety through mPFC neural activity, providing novel insights into microbiome-based interventions for anxiety.…”
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Identification Exploring the Mechanism and Clinical Validation of Mitochondrial Dynamics-Related Genes in Membranous Nephropathy Based on Mendelian Randomization Study and Bioinfor... by Qiuyuan Shao, Nan Li, Huimin Qiu, Min Zhao, Chunming Jiang, Cheng Wan

“…The data underscore the interplay between mitochondrial dynamics and immune dysregulation in MN progression, providing a foundation for precision medicine strategies.…”
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SIRT1 prevents noise-induced hearing loss by enhancing cochlear mitochondrial function by Yuelian Luo, Haoyang Wu, Xin Min, Yi Chen, Wenting Deng, Minjun Chen, Chuxuan Yang, Hao Xiong

“…Collectively, these findings indicate that SIRT1 is essential for the maintenance of redox balance and mitochondrial function in the cochlea after traumatic noise exposure, thus providing a promising therapeutic target for NIHL treatment. …”
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Genome-wide characterization and their roles in abiotic stress responses of HD-Zip transcription factors in pepper by Xiaoqin Wang, Xiaoya Zhou, Kunhao Xie, Xiaojie Feng, LuLiu, Lihong Gao, Mintao Sun, Yansu Li, Chaoxing He

“…Subsequent stress simulation assays and qRT-PCR validation confirmed the reliability of transcriptomic findings. Conclusion This study delivers the first systematic exploration of HD-Zip transcription factors in Capsicum annuum under abiotic stress, providing foundational knowledge and candidate genes for improving stress resilience in pepper breeding programs.…”
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Comparative Analysis of Microbial–Short-Chain Fatty Acids–Epithelial Transport Axis in the Rumen Ecosystem Between Tarim Wapiti (<i>Cervus elaphus yarkandensis</i>) and Karakul She... by Jianzhi Huang, Yueyun Sheng, Xiaowei Jia, Wenxi Qian, Zhipeng Li

“…These evolutionary adaptations, particularly the Bacteroidetes–short-chain fatty acids–transporter axis, likely underpin the wapiti’s superior roughage utilization efficiency, providing molecular insights for improving ruminant feeding strategies in an arid environment.…”
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Identification of StCCoAOMT gene family and analysis of resistance of StCCoAOMT1 and StCCoAOMT7 genes to drought, alkaline salt and combined stresses in potato by Yunyun Du, Ruyan Zhang, Yuan Lu, Yong Wang, Xingxing Wang, Weina Zhang, Yichen Kang, Yuhui Liu, Shuhao Qin

“…The results indicate that both proteins may function in the nucleus, plasma membrane, and cytoplasm, providing new insights into the molecular mechanisms underlying plant defense and stress responses. …”
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Periplocin induces necroptosis in papillary thyroid carcinoma through DR4 mediated RIPK3 and MLKL signaling by Li Ding, Fengmei Wen, Liang Zeng, Ziwei Li, Gangyin Xie

“…RNA-seq was conducted to compare the transcriptomes of periplocin-treated and untreated TPC-1 cells. …”
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Single-cell RNA sequencing reveals a B cell-related immunosuppressive landscape and a potential suppressor in hepatocellular carcinoma by Wenhao Hou, Jiaqi Jiang, Liyuan Zhu, Hongwei Xu, Lihui Zhu

“…Methods To characterize the HCC microenvironment, the single-cell transcriptomes of HCC tissues and corresponding noncancerous tissues were analysed. …”
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Maternal exposure to difenoconazole-induced developmental defects and its mechanisms in F1 zebrafish (Danio rerio) by Ying Zhang, Fuqi Yang, Qian Zhang, Yunan Wu, Jiaolong Huang, Peng Duan, Xingjian Zhou

“…Comprehensive phenotypical and molecular assessments were subsequently performed on F1 progeny, incorporating physiological biomarkers evaluation, histological examination, transcriptomic profiling, and quantitative validation of dysregulated signaling pathways. …”
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Ectopic expression of GDF15 in cancer-associated fibroblasts enhances melanoma immunosuppression via the GFRAL/RET cascade by Zhiheng Lin, Li Lin, Yisheng Chen, Yantao Ding, Xiaojing Wang, Zhiwen Luo, Zhenzhen Zhao, Zhijie Zhao, Huabao Cai, Wenyang Nie, Fu Zhao

“…This cascade reaction ultimately induces macrophage polarization to the immunosuppressive M2 phenotype, assists in the establishment of an immunosuppressive microenvironment, and leads to accelerated melanoma lung metastasis.Conclusion By integrating single-cell RNA-seq, spatial transcriptomics, bulk RNA-seq, molecular dynamics simulation and complete experimental design, this study comprehensively characterized that ectopic expression of CAFs-derived GDF15 can act as an accomplice in melanoma progression by inducing increased tumor cell stemness and macrophage M2 polarization, reshaping the immune landscape of melanoma, and providing new ideas and new targets for precision immunotherapy of melanoma.…”
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Intratumoral Collagen Deposition Supports Angiogenesis Suggesting Anti‐angiogenic Therapy in Armored and Cold Tumors by Jie Mei, Kai Yang, Xinkang Zhang, Zhiwen Luo, Min Tian, Hanfang Fan, Jiahui Chu, Yan Zhang, Junli Ding, Junying Xu, Yun Cai, Yongmei Yin

“…Single‐cell and spatial transcriptomics reveal close interactions and spatial co‐localization of fibroblasts and endothelial cells. …”
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Degenerated vision, altered lipid metabolism, and expanded chemoreceptor repertoires enable Lindaspio polybranchiata to thrive in deep-sea cold seeps by Yujie Yan, Inge Seim, Yang Guo, Xupeng Chi, Zhaoshan Zhong, Dantong Wang, Mengna Li, Haining Wang, Huan Zhang, Minxiao Wang, Chaolun Li

“…Supporting this, transcriptomic and fatty acid data further corroborate our findings. …”
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