Cross-species single-cell RNA-seq analysis reveals disparate and conserved cardiac and extracardiac inflammatory responses upon heart injury by Eric Cortada, Jun Yao, Yu Xia, Friederike Dündar, Paul Zumbo, Boris Yang, Alfonso Rubio-Navarro, Björn Perder, Miaoyan Qiu, Anthony M. Pettinato, Edwin A. Homan, Lisa Stoll, Doron Betel, Jingli Cao, James C. Lo

“…Our study furnishes a direct cross-species comparison of immune responses between regenerative and profibrotic myocardial injury models, providing a useful resource to the fields of regenerative biology and cardiovascular research.…”
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Integrative single-cell RNA sequencing and bulk RNA sequencing reveals the characteristics of glutathione metabolism and protective role of GSTA4 gene in pancreatic cancer by Xinya Jia, Qiang Zhang, Zhe Wang, Jianliang Cao, Anran Song, Chao Lan, Yuepeng Hu

“…Utilizing single-cell transcriptomic data from the GSE205049 dataset, this study analyzed the variation in GSH metabolic signaling intensity across distinct cell types within the tumor microenvironment of PC. …”
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Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration by Zhangrong Cheng, Haiyang Gao, Pengzhi Shi, Anran Zhang, Xianglong Chen, Yuhang Chen, Weikang Gan, Kangcheng Zhao, Shuai Li, Cao Yang, Yukun Zhang

“…This study elucidates CMA’s regulatory role in SASP-mediated senescence amplification circuits, providing novel therapeutic insights for IDD and other age-related pathologies.…”
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NcROP2 deletion reduces Neospora caninum virulence by altering parasite stage differentiation and hijacking host immune response by Rafael Amieva, Montserrat Coronado, Jessica Powell, David Arranz-Solís, Musa A. Hassan, Esther Collantes-Fernández, Luis Miguel Ortega-Mora, Pilar Horcajo

“…Additionally, the NcΔROP2 mutants exhibited impaired proliferation and significantly induced the expression of interferon-stimulated genes in bovine monocyte-derived macrophages infected in vitro for 60 hours. Transcriptomic analysis further revealed a shift in parasite gene expression, with an upregulation of stress-related and bradyzoite markers. …”
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Metrnl/Meteorin-like/IL-41, a novel regulator of bone metabolism and disease activity in ankylosing spondylitis: based on multi-omics analysis by Zhuoqi Li, Zhuoqi Li, Tao Sun, Min Zhao, Liping Xia, Hui Shen

“…Besides, Metrnl plays a critical role in regulating cartilage and bone metabolism and maintaining bone homeostasis, providing new insights for the future diagnosis and treatment of bone-related diseases.…”
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Morpho-Physiological and Molecular Responses to Seedling-Stage Drought Stress in Different Cowpea Cultivars by Inocent Paulin Ritte, Marceline Egnin, Gregory Christopher Bernard, Desmond Mortley, Osagie Idehen, Michelle Pamelas Okoma, Conrad Bonsi

“…Future research using transcriptomic profiling could unravel the complex molecular mechanisms of drought responses in cowpeas, providing valuable insights for breeding genotypes with improved resiliency to drought.…”
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Development and validation of a hypoxia-immune-based microenvironment gene signature for predicting survival in non-small cell lung cancer by Xiangwu Zhang, Rongxian Zhou, Guangqiang Zhao, Wanling Chen, Ping Zhao, Yunchao Huang, Qiubo Huang, Lianhua Ye

“…Conclusions The hypoxia-immune microenvironment signature serves as a robust prognostic classifier for NSCLC, providing a quantitative framework for personalized risk stratification and clinical decision support.…”
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The Potential Role of C4 MYH11+ Fibroblasts and the MDK-SDC2 Ligand-Receptor Pair in Lung Adenocarcinoma: Implications for Prognosis and Therapeutic Strategies by Hongling Jia, Yanjie Bian, Jie Yuan, Yi Zhang, Shengyi Zhang

“…Additionally, we developed a gene-based prognostic model that effectively predicted patient survival, providing valuable clinical insights. Conclusion: This study provided a comprehensive atlas of the LUAD tumor microenvironment, highlighted the role of the C4 MYH11+ Fibroblasts in tumor progression. …”
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Migrasome Marker Epidermal Growth Factor Domain-Specific <i>O</i>-GlcNAc Transferase: Pan-Cancer Angiogenesis Biomarker and the Potential Role of circ_0058189/miR-130a-3p/EOGT Axis... by Zhe Yu, Jing Luo, Wen An, Herui Wei, Mengqi Li, Lingling He, Fan Xiao, Hongshan Wei

“…<b>Methods</b>: Multi-omics analysis integrated TCGA/GTEx data from 33 cancer types with spatial/single-cell transcriptomics and 10 HCC cohorts. Functional validation employed Huh7 cell models with <i>EOGT</i> modulation, RNA sequencing, and ceRNA network construction. …”
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High-altitude hypoxia exacerbates chemotherapy-induced myelosuppression by lowering serum G-CSF/GM-CSF and regulating apoptosis and proliferation by Jing Shi, Fuxing Zhao, Tianlei Qiu, Dengfeng Ren, Zitao Li, Junli Ma, Jiuda Zhao

“…This study aimed to investigate the effects and potential mechanisms of high-altitude hypoxia on myelosuppression following chemotherapy, with the goal of providing a theoretical basis for clinical treatment. …”
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An interpretable survival model for diffuse large B-cell lymphoma patients using a biologically informed visible neural network by Jie Tan, Jiancong Xie, Jiarong Huang, Weizhen Deng, Hua Chai, Yuedong Yang

“…However, existing DLBCL prognostic models are mainly based on transcriptomic profiles, while genetic variation detection is more commonly used in clinical practice. …”
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Dual Proteomics Strategies to Dissect and Quantify the Components of Nine Medically Important African Snake Venoms by Damien Redureau, Fernanda Gobbi Amorim, Thomas Crasset, Imre Berger, Christiane Schaffitzel, Stefanie Kate Menzies, Nicholas R. Casewell, Loïc Quinton

“…The culmination of these efforts resulted in the construction of a detailed toxin database, providing insights into the biological roles and potential therapeutic targets of venom proteins and peptides. …”
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Functional Verification of Differentially Expressed Genes Following DENV2 Infection in <i>Aedes aegypti</i> by Xiaoli Chen, Xinyu Zhou, Xiaoxue Xie, Bo Li, Teng Zhao, Haotian Yu, Dan Xing, Jiahong Wu, Chunxiao Li

“…Investigating genes associated with mosquito susceptibility to DENV2 offers a theoretical foundation for targeted interventions to regulate or block viral replication and transmission within mosquitoes. Based on the transcriptomic analyses of the midgut and salivary glands from <i>Aedes aegypti</i> infected with DENV2, alongside analyses of Aag2 cell infections, 24 genes potentially related to the regulation of <i>Aedes aegypti</i> infection with DENV2 were selected. …”
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Integrated omics profiles for exploring the potential mechanism underlying aroma formation in the terpenoid-rich aromatic plant Opisthopappus taihangensis and the bioactivity of it... by Maolin Liu, Yushu Li, Haixia Chen, Chunjie He, Lei Sun, Xiuhai Zhang, Zongda Xu, Hua Liu

“…In this study, we conducted comprehensive metabolomic and transcriptomic analyses to identify changes in terpenoid metabolites and associated gene expression across various O. taihangensis tissues (leaves, buds, and inflorescences at the exposure, initial-bloom, and full-bloom stages). …”
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MYC promotes group 3 medulloblastoma cell proliferation and alleviates ROS-induced cell death by upregulating transketolase by Shizun Wang, Dan Zhang, Chunlong Wang, Yuqin Liu

“…In this study, we utilized transcriptomic data to identify that high expression of transketolase (TKT) correlates with worse overall survival (OS) in Group 3 patients. …”
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Immune Characters and Plasticity of the Sentinel Lymph Node in Colorectal Cancer Patients by Xiaoyun Li, Jingling Tang, Hang Du, Xinjun Wang, Liyun Wu, Pingsheng Hu, Hua Zhang, Ruyi Zhang, Yuan Yang

“…The ex vivo culture enhanced antitumor immunological function of SN-T cells, providing a starting material for adoptive cell therapy for CRC.…”
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PI3Kδ/γ inhibitor BR101801 extrinsically potentiates effector CD8+ T cell-dependent antitumor immunity and abscopal effect after local irradiation by Tae-Jin Kim, Yi Na Yoon, Jae-Sung Kim, Eunju Lee, Young-Ju Kwon, Jeong-An Gim

“…Furthermore, the high ratio of CD8α+ T cells to Tregs was maintained for 14 days after irradiation, resulting in remote tumor regression in metastatic lesions, the so-called abscopal effect. Moreover, our transcriptomic analysis showed that BR101801 combined with irradiation promoted the immune-stimulatory tumor microenvironment, suggesting that the combined therapy converts immunologically cold tumors into hot one.Conclusions Our data suggest the first evidence that PI3Kδ/γ inhibition combined with irradiation promotes systemic antitumor immunity against solid tumors, providing the preclinical result of the potential use of PI3Kδ/γ inhibitor as an immune-regulatory radiosensitizer.…”
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Methylation patterns of the nasal epigenome of hospitalized SARS-CoV-2 positive patients reveal insights into molecular mechanisms of COVID-19 by Benjamin L Spector, Boryana Koseva, Rebecca McLennan, Dithi Banerjee, Kamani Lankachandra, Todd Bradley, Rangaraj Selvarangan, Elin Grundberg

“…Efforts to identify factors responsible for differential COVID-19 severity include but are not limited to genome wide association studies (GWAS) and transcriptomic analysis. More recently, variability in host epigenomic profiles have garnered attention, providing links to disease severity. …”
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Investigation regarding the effects of different monochromatic lights on lipid metabolism and immune function in chickens by Xiaodong Tan, Yuting Jin, Jiahua Li, Jie Dong, Minjie Huang, Deqian Wang

“…This study reveals potential pathways through which light affects lipid metabolism and immune function, providing new insights into how light influences animal development and production.…”
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Screening and regulatory mechanism exploration of M1 macrophage polarization and efferocytosis-related biomarkers in coronary heart disease by Hong Gao, Junhua Li, Jianxin Huang, Xiaojie Jiang

“…This study aimed to identify CHD-associated biomarkers using transcriptomic data.MethodsThis study examined 74 efferocytosis-related genes (ERGs) and 17 M1 macrophage polarization-related genes (MRGs) across two CHD-relevant datasets, GSE113079 and GSE42148. …”
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