Cloning and Bioinformatics Analysis of Vitellogenin Genes and Its Receptor Genes in Thalassodes immissaria by Guolin ZHU, Qiong YAO, Wenjing LI, Shu XU, Kai LIU, Shuanggang DUAN

“…【Objective】Vitellogenin (Vg) and its receptor (VgR) play key roles in insect reproduction by mediating nutrient transport during oocyte vitellogenesis.This study aimed to clone Vg and VgR from the Thalassodes immissaria, a notorious defoliator in lichee, and analyzed their bioinformatics characteristics and expression patterns across developmental stages to infer their roles in reproductive regulation.【Method】Using transcriptome data of T.immissaria, the full-length cDNA sequences of TiVg and TiVgR were cloned via PCR.Bioinformatics tools, such as ORF Finder, SignalP 5.0, ProtParam, SMART, TMHMM 2.0, and MEGA v5.05, were used for sequence analysis.Expression profiles of TiVg and TiVgR across different developmental stages and different tissues were quantified using transcriptomic TPM values and qRT-PCR.…”
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Cellular senescence induced by cholesterol accumulation is mediated by lysosomal ABCA1 in APOE4 and AD by Shaowei Wang, Boyang Li, Jie Li, Zhiheng Cai, Cristelle Hugo, Yi Sun, Lu Qian, Julia TCW, Helena C. Chui, Dante Dikeman, Isaac Asante, Stan G. Louie, David A. Bennett, Zoe Arvanitakis, Alan T. Remaley, Bilal E. Kerman, Hussein N. Yassine

“…Methods We examined the association between cellular senescence and ABCA1 expression in human postmortem brain samples using transcriptomic, histological, and biochemical analyses. …”
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Genome-Wide Identification and Expression Analysis of the Mango (<i>Mangifera indica</i> L.) SWEET Gene Family by Lirong Zhou, Xinyu Liu, Xiangchi Leng, Meng Zhang, Zhuanying Yang, Wentian Xu, Songbiao Wang, Hongxia Wu, Qingzhi Liang

“…The results of this study provide a new way to further study the regulation of mango SWEET family genes on sugar accumulation, highlight their potential role in fruit quality improvement, and lay an important foundation for further study of mango SWEET function and enhance mango competitiveness in fruit market.…”
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Deep learning enabled integration of tumor microenvironment microbial profiles and host gene expressions for interpretable survival subtyping in diverse types of cancers by Haohong Zhang, Xinghao Xiong, Mingyue Cheng, Lei Ji, Kang Ning

“…In this study, we aimed to decode this complex relationship by developing ASD-cancer (autoencoder-based subtypes detector for cancer), a semi-supervised deep learning framework that could extract survival-related features from tumor microbiome and transcriptome data, and identify patients’ survival subtypes. …”
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Identification of a potential homeodomain-like gene governing leaf size and venation architecture in birch by Xiuyan Bian, Xiuyan Bian, Chen Chen, Chen Chen, Yang Wang, Yang Wang, Chang Qu, Chang Qu, Jing Jiang, Yao Sun, Guifeng Liu

“…Meanwhile, reduced expression levels of BpPHD4 could remarkably promote phloem tissue development. Transcriptome analysis of BpPHD4 overexpression transgenic plants showed two candidate genes (Bpev01.c0518.g0018 and Bpev01.c2797.g0002) probably regulated by BpPHD4. …”
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Screening of ent-copalyl diphosphate synthase and metabolic engineering to achieve de novo biosynthesis of ent-copalol in Saccharomyces cerevisiae by Shan Li, Shuangshuang Luo, Xinran Yin, Xingying Zhao, Xuyang Wang, Song Gao, Sha Xu, Jian Lu, Jingwen Zhou

“…In this study, de novo biosynthesis and identification of ent-copalol were achieved and the highest titer ever reported. It provides a platform strain for the further pathway analysis of andrographolide and derivatives and provides a reference for the synthesis of other pharmaceutical intermediates.…”
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OXidative Stress PREDictor: A Supervised Learning Approach for Annotating Cellular Oxidative Stress States in Inflammatory Cells by Po‐Yuan Chen, Tai‐Ming Ko

“…Overall, OxSpred enhances the utility of single‐cell transcriptomic datasets by providing a robust in silico method for determining intracellular oxidative stress states, thereby enriching the understanding of innate immune cell functions during inflammation.…”
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Deep learning and genome-wide association meta-analyses of bone marrow adiposity in the UK Biobank by Wei Xu, Ines Mesa-Eguiagaray, David M. Morris, Chengjia Wang, Calum D. Gray, Samuel Sjöström, Giorgos Papanastasiou, Sammy Badr, Julien Paccou, Xue Li, Paul R. H. J. Timmers, Maria Timofeeva, Susan M. Farrington, Malcolm G. Dunlop, Scott I. Semple, Tom MacGillivray, Evropi Theodoratou, William P. Cawthorn

“…Finally, we perform a multi-ancestry meta-GWAS to identify genes associated with bone marrow adiposity across the different bone regions and across ancestry groups. Our findings provide insights into BMAT formation and function and provide a basis to study the impact of BMAT on human health and disease.…”
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Bioinformatics in Age-Related Macular Degeneration: A Narrative Review by Mehrdad MOTAMED SHARIATI

“…Conclusion: Bioinformatics is revolutionizing AMD research by providing insights into its molecular basis, enabling early diagnosis, and aiding in the development of personalized therapies. …”
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Protein landscape of the brush border membrane of first instar larvae of Frankliniella occidentalis, the western flower thrips. by Marlonni Maurastoni, Dorith Rotenberg, Ruchir Mishra, Bryony C Bonning, Anna E Whitfield

“…Bioinformatic-enabled comparisons to thrips tissue-specific proteomes and transcriptomes enabled us to predict the secretome and the plasma membrane proteins of larval thrips' gut epithelial cells, providing new targets for thrips control.…”
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Potential candidate genes influencing meat production phenotypic traits in sheep: a review by Ying Han, Muhammad Faheem Akhtar, Wenting Chen, Xiaotong Liu, Mingyue Zhao, Limeng Shi, Muhammad Zahoor Khan, Changfa Wang

“…This comprehensive genetic marker catalog serves as a critical resource repository for implementing marker-assisted selection programs, providing breeders and researchers with validated genetic targets to accelerate breeding efficiency and enhance meat production in sheep worldwide.…”
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Tetramethyl bisphenol F exposure induces human ovarian granulosa cell senescence and mouse ovarian damage by regulating ESRRB signalling by Huiyu Fan, Chunming Li, Yifei Lv, Tongyun Qi, Yizhou Huang, Linjuan Ma, Yibing Lan, Peiqiong Chen, Zheng Lou, Jianhong Zhou

“…Recent research has highlighted possible health risks associated with TMBPF, yet its impact on ovarian function and the mechanisms involved remain inadequately understood. This study provides a thorough evaluation of TMBPF's effects on granulosa cells (GCs) and uncovers its transgenerational influence on female reproductive health. …”
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Dendritic cell vaccination and CD40-agonist combination therapy licenses T cell-dependent antitumor immunity in a pancreatic carcinoma murine model by Sjoerd H van der Burg, Thorbald van Hall, Heleen Vroman, Menno van Nimwegen, Christianne Groeneveldt, Priscilla Kinderman, Dana A M Mustafa, Nadine van Montfoort, Joachim Aerts, Melanie Lukkes, Sai Ping Lau, Casper H J van Eijck, Larissa Klaase, Mandy van Gulijk, Jasper Dumas, Sanne L A Lievense, Ralph Stadhouders, Yunlei Li, Andrew Stubbs, Koen A Marijt, Floris Dammeijer

“…Combination therapy induced a strong change in tumor transcriptome and mitigated the expression of inhibitory markers on CD8 +T cells.Conclusion These results demonstrate the potency of DC therapy in combination with CD40-stimulation for the treatment of pancreatic cancer and provide directions for near future clinical trials.…”
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Transcriptional dynamics and functions of WUSCHEL-related homeobox (WOX) genes from Ginkgo biloba in tissue culture by Wei Xu, Ang Xu, Pingjun Xu, Jiaqi Li, Chao Luo, Xiaoming Yang, Meiling Ming, Yuhua Liu, Guibin Wang, Liangjiao Xue, Fuliang Cao, Gaiping Wang, Fangfang Fu

“…Conclusions This study provides the first comprehensive analysis of the WOX gene family in Ginkgo biloba and highlights its significant role in tissue culture regeneration. …”
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Lnc MSTRG 4701.7 targets miR-1786/RORa to competitively regulate proliferation and apoptosis in chicken follicular granulosa cells by Chang Ma, Chang Ma, Hengsong Wu, Hengsong Wu, Chunchi Yan, Chunchi Yan, Simushi Liswaniso, Simushi Liswaniso, Xue Sun, Xue Sun, Ning Qin, Ning Qin, Rifu Xu, Rifu Xu

“…Here, we aimed to identify key differentially expressed LncRNAs and explore their regulatory roles in follicular development, particularly focusing on the MSTRG.4701.7-miR-1786-RORa axis, to provide insights for improving fertility in low-fertility chicken varieties.MethodsIntact follicular tissues were collected from Jilin Black chickens (low-fertility group) and Lohmann Brown Laying hens (high-fertility group) at each stage of ovarian development for transcriptome sequencing to identify key differentially expressed LncRNAs for follow-up analyses. …”
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Identification and functional analysis of AvLACS genes unveils their role in lipid homeostasis and waterlogging tolerance in kiwifruit (Actinidia valvata Dunn) by Meijuan Zhang, Kaiyu Ye, Jianyou Gao, Shibiao Liu, Ruonan Liu, Ruonan Liu, Yuexing Chen, Qian Zhao, Yushan Lei, Jiewei Li, Cuixia Liu, Faming Wang

“…These integrated molecular and biochemical data provide mechanistic insights highlighting distinct and coordinated roles of AvLACSs in lipid metabolic remodeling under waterlogging stress. …”
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A Novel ceRNA Axis LOC121818100/Novel‐miR‐400/SSRP1 Regulated Muscle Growth and Injury Repair in Sheep by Fan Yang, Runqing Chi, Runan Zhang, Kai Liu, Pingqing Wang, Ran Di, Xiaoyun He, Xiangyu Wang, Yufang Liu, Mingxing Chu

“…The construction of competitive endogenous RNA (ceRNA) networks provides an effective way to analyse the molecular mechanism of complex trait formation. …”
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Enhanced efficacy of immune checkpoint inhibitors by folate-targeted multifunctional drug through synergistic therapy inducing ferroptosis and immunogenic cell death in bladder can... by Yibo Shi, Guangrui Fan, Enguang Yang, Yuanfeng Zhang, Hui Ding, Junqiang Tian, Liang Cheng, Hanzhang Wang, Tianzhi Hao, Baodui Wang, Zhiping Wang

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A novel Tc17 population recruited by tumor cells promotes tumor progression in gastric cancer by Yichao Wang, Yichao Wang, Hao Yuan, Yayun Tan, Li Wu, Li Wu, Xinchen Zhao, Xinchen Zhao, Zeyuan Yang, Zeyuan Yang, Shaoxing Dai, Shaoxing Dai, Naixue Yang, Naixue Yang

“…We found that tumor cells can recruit Tc17 cells via the CXCL16-CXCR6 axis, this finding was validated on spatial transcriptome data. And in turn, Tc17 cells may promote tumor progression by secreting IL-17A and IL-26, as functionally corroborated by CCK-8 Assay, wound healing, and transwell assay in vitro.ConclusionsThese findings reveal the immunosuppressive role of Tc17 cells in gastric cancer, provide a new perspective for understanding the immunosuppressive mechanism of the gastric cancer tumor microenvironment, and provide a potential target for the development of targeted therapeutic strategies…”
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Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction by Jiewu Huang, Ping Meng, Ye Liang, Xiaolong Li, Shan Zhou, Jiemei Li, Xiaoxu Wang, Jinhua Miao, Weiwei Shen, Lili Zhou

“…CD44 also facilitated iron intake to assist cell ferroptosis. Hence, our study provided a new mechanism for AKI, and demonstrated that targeted inhibition on CD44 could be a promising therapeutic strategy to resist AKI.…”
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