The dual role of CXCL9/SPP1 polarized tumor-associated macrophages in modulating anti-tumor immunity in hepatocellular carcinoma by Yu Gu, Zhihui Zhang, Hao Huang, Wenyong Zhu, Hongjia Liu, Rongxin Zhang, Nan Weng, Xiao Sun

“…Reprogramming tumor-associated macrophages (TAMs) into an anti-tumor phenotype is a promising strategy.MethodsA comprehensive analysis by combing multi-regional single-cell, bulk and spatial transcriptome profiling with radiomics characterization was conducted to dissect the heterogeneity of TAMs and resolve the landscape of the CXCL9:SPP1 (CS) macrophage polarity in HCC.ResultsTAMs were particularly increased in HCC. …”
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Distinctive features of IncRNA and mRNA between severe and mild patients with influenza a (H1N1) virus pneumonia by Dangsheng Xiao, Jinyou Li, Xuehui Zhao, Yongtao Li, Haifeng Lu, Jiezuan Yang

“…Understanding the molecular differences between severe and mild cases can provide insights into the pathogenesis and potential therapeutic targets for H1N1 infections. …”
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The Spatial‐Temporal Alternative Splicing Profile Reveals the Functional Diversity of FXR1 Isoforms in Myogenesis by Wei Wang, Xinhao Fan, Weiwei Liu, Yuxin Huang, Shuhong Zhao, Yalan Yang, Zhonglin Tang

“…Collectively, these findings provide a comprehensive resource for AS studies in skeletal muscle development, underscoring the diverse functions and regulatory mechanisms governing distinct Fxr1 isoforms in myogenesis.…”
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Revitalizing the Epigenome of Adult Jaw Periosteal Cells: Enhancing Diversity in iPSC-Derived Mesenchymal Stem Cells (iMSCs) by Felix Umrath, Valerie Wendt, Gilles Gasparoni, Yasser Narknava, Jörn Walter, Bernd Lethaus, Josefin Weber, Victor Carriel, Meltem Avci-Adali, Dorothea Alexander

“…Additionally, this analysis provided an opportunity for us to investigate the potential rejuvenation effects associated with cellular reprogramming. …”
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Dynamic Alterations in DNA Methylation of CD4+ T Cells and Macrophages in a Murine Model of Tuberculous Pleural Infection Induced by BCG Vaccination by Ming‐Ming Shao, Qing‐Yu Chen, Xin Zhang, Shu‐Feng Dong, Rui‐Qi Wei, Huan‐Zhong Shi, Feng‐Shuang Yi

“…This study analyzed DNA methylation and transcriptome data from macrophages and CD4+ T cells from pleural lavage fluid of BCG‐induced tuberculous pleurisy mouse models at specific time points (Days 0, 1, 7, and 14). …”
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Matrix Metalloproteinase‐Responsive Controlled Release of Self‐Assembly Nanoparticles Accelerates Heart Valve Regeneration In Situ by Orchestrating Immunomodulation by Xing Chen, Yunlong Wu, Peng Song, Liandong Feng, Ying Zhou, Jiawei Shi, Nianguo Dong, Weihua Qiao

“…In vivo, this valve scaffold promises to mitigate early inflammatory damage and provide a pro‐endothelialization niche for scaffolds' constructive remodeling. …”
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Large-scale RNA-Seq mining reveals ciclopirox olamine induces TDP-43 cryptic exons by Irika R. Sinha, Parker S. Sandal, Holly Spence, Grace D. Burns, Aswathy Peethambaran Mallika, Fatemeh Abbasinejad, Katherine E. Irwin, Anna Lourdes F. Cruz, Vania Wang, Shaelyn R. Marx, Josué Llamas Rodríguez, Ben Langmead, Jenna M. Gregory, Philip C. Wong, Jonathan P. Ling

“…We established cryptic exon inclusion levels across a variety of human cells and tissues to provide ground truth references for future studies on TDP-43 dysregulation. …”
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The extraction of effective components and an antioxidant activity study of Tulipa edulis by Doudou Zhang, Dong Xiao, Tingting Yin, Shuangzhi Zhao, Olena Zhur, Xun Xiao, Hailun He, Leilei Chen

“…Meanwhile, the gene expression of heme oxygenase 1 (HO-1), γ-glutamyl cysteine ligase catalytic subunit (GCLC) and NAD(P)H quinone oxidoreductase-1 (NQO1) in HUVEC cells was up-regulated after treated with F2. This study provides reference value for the further development and application of T. edulis and the development of functional food.…”
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Aux/IAA gene family identification and analysis reveals roles in flower opening and abiotic stress response in Osmanthus fragrans by Shanshan Cao, Yong Ye, Zifei Zheng, Shiwei Zhong, Yiguang Wang, Zheng Xiao, Qiu Fang, Jinping Deng, Hongbo Zhao, Bin Dong

“…Analysis of cis-regulatory elements (CREs) in the promoters of the OfIAA genes revealed the presence of many CREs related to different hormones and abiotic stresses. Through transcriptome and expression pattern analysis, we found that the majority of OfIAA genes were expressed in the stem tissue. …”
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Insights into the special physiology of Mortierella alpina cultured by agar supported solid state fermentation in enhancing arachidonic acid enriched lipid production by Tingting Liu, Pandeng Li, Ziqi Ou, Yumei Feng, Bohan Wang, Tianyi Yu, Yuanmin Zhu, Longjiang Yu

“…This study demonstrated that agar-supported SSF (AgSF) could induce Mortierella alpina M0223 to yield higher ARA-rich lipids than submerged fermentation (SmF), and elucidated the underlying mechanisms by the comparative transcriptome. AgSF-M0223 formed a mycelial network consisting mainly of surface (SH) and aerial hyphae (AH). …”
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Transcriptional search to identify and assess reference genes for expression analysis in Solanumlycopersicum under stress and hormone treatment conditions by Yao-ke DUAN, Rong HAN, Yan SU, Ai-ying WANG, Shuang LI, Hao SUN, Hai-jun GONG

“…Our study suggests that excavating stably expressed genes from transcriptome sequencing data is a reliable approach to screening reference genes for qPCR analysis.…”
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Does manipulation of phyto-based nanoparticles is a promising solution against multi-drug resistant (MDR) pathogens? A critical opinion towards tackling MDR pathogens by Subhasmita Mallik, Samikshya Nayak, Arpita Panda, Smrutirekha Behera, Kamalakanta Swain, Archita Patra, Rukmini Mishra, Jatindra Nath Mohanty

“…It also examined the effectiveness of phytobased nanoparticles in drug delivery systems to treat MDR infections; both alone and in combination with antibiotics, and provided a critical assessment of their potentials as a research avenue. …”
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Biosafety Evaluation of a Chimeric Adenoviral Vector in Mini-Pigs: Insights into Immune Tolerance and Gene Therapy Potential by Andrei Izmailov, Irina Minyazeva, Vage Markosyan, Zufar Safiullov, Ilnaz Gazizov, Ilnur Salafutdinov, Maria Markelova, Ravil Garifulin, Maksim Shmarov, Denis Logunov, Rustem Islamov, Vadim Pospelov

“…Conclusions: Here, we show the safety of Ad5/35F in a mini-pig model and provide evidence that Ad5/35F is a promising vector for gene therapy. …”
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TGF-β3 Restrains Osteoclastic Resorption Through Autophagy by Hui Liao, Yiqin Pan, Yiming Liu, Yuxiao Li, Shiyi Huang, Shan Ding, Qi Xiang

“…Analysis of the full transcriptome of hPDLSCs (human periodontal ligament stem cells) revealed that the expression of RANKL was significantly up-regulated after TGF-β3 treatment during osteogenesis, which suggests its involvement in clock-controlled autophagy in bone metabolism. …”
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Gut microbiota modulate radiotherapy-associated antitumor immune responses against hepatocellular carcinoma Via STING signaling by Zongjuan Li, Yang Zhang, Weifeng Hong, Biao Wang, Yixing Chen, Ping Yang, Jian Zhou, Jia Fan, Zhaochong Zeng, Shisuo Du

“…We used fecal microbiota transplantation (FMT), flow cytometry, and transcriptome sequencing to explore the effects of dysbiosis on RT. …”
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Sele-targeted siRNA liposome nanoparticles inhibit pathological scars formation via blocking the cross-talk between monocyte and endothelial cells: a preclinical study based on a n... by Luyu Li, Yong Wang, Jing Meng, Xue Wang, Xiaojin Wu, Yan Wo, Ying Shang, Zhen Zhang

“…RNA sequencing identified significant transcriptome changes in RSM-induced scars. Elevated expression of E-Selectin (Sele) was observed in endothelial cells from both the RSM model and human PS (Keloid) samples. …”
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Leveraging diverse cell-death patterns in diagnosis of sepsis by integrating bioinformatics and machine learning by Mi Liu, Xingxing Gao, Hongfa Wang, Yiping Zhang, Xiaojun Li, Renlai Zhu, Yunru Sheng

“…Methods Fourteen PCD patterns were analyzed for model construction. Seven transcriptome datasets and a single cell sequencing dataset were collected from the Gene Expression Omnibus database. …”
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Molecular regulatory mechanisms of dietary supplementation with Regel powder to improve muscle development and meat quality in Angus calves by Wangjing Liu, Chenxu Sun, Huixia Gao, Jianjian He, Aihuan Yu, Yaodi Xie, Haibo Yao, Jiang Hu, Zhaomin Lei

“…It also significantly modulated meat quality, possibly altering signaling pathways by influencing key gene expression. Our results provide novel insights into the development of the meat industry and indicate the mechanism through which AMRP regulates muscle development and improves meat quality at the molecular level.…”
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Exogenous EBR enhanced heat tolerance in mini Chinese cabbage by regulating ABA accumulation by Jiaojiao Yang, Guangzheng Wang, Yongmei He, Wenxu Chen, Xuehua Wang, Jizhong Ma, Xueqing Gao, Jihua Yu, Linli Hu

“…In conclusion, exogenous EBR can improve the heat resistance of mini Chinese cabbage by regulating ABA accumulation. This study provides new insights into the potential mechanism of EBR alleviating heat stress in mini Chinese cabbage.…”
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Effects of the FHL2 gene on the development of subcutaneous and intramuscular adipocytes in goats by An Li, Youli Wang, Yong Wang, Yan Xiong, Yanyan Li, Wei Liu, Jiangjiang Zhu, Yaqiu Lin

“…Four and half-LIM protein (FHL2) is positively correlated with the hypertrophy of adipocytes and can cause symptoms such as obesity and diabetes. Result In the transcriptome sequencing analysis of intramuscular adipocytes after three days of differentiation, the differentially expressed gene FHL2 was found. …”
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