Physiological and molecular profiling unveils oat (Avena sativa L.) defense mechanisms against powdery mildew by Aijie Ma, Aijie Ma, Aijie Ma, Tao Liu, Tao Liu, Wenhui Tian, Wenhui Tian, Wenhui Tian, Hong Chen, Hong Chen, Hong Chen, Guoqing Wang, Guoqing Wang, Guoqing Wang, Bo Zhang, Bo Zhang, Bo Zhang

“…These findings suggest that BY642 employs a coordinated defense strategy integrating ROS dynamics, SA signaling, and transcriptional regulation, providing novel insights into the molecular basis of powdery mildew resistance and potential targets for oat breeding.…”
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Functional analysis of NtPDX2 in Nicotiana tabacum L. associated with stem development by Li Xu, Yuxin Cui, Jiaxin Xing, Qili Mi, Zhixing Wang, Xujing Wang, Wanli Zeng, Haiying Xiang, Jiarui Jiang, Lele Deng, Kunmiao Wang, Jiangtao Yang, Qian Gao

“…The overexpressing plants exhibited a significant increase in height (100.93 cm) and stem thickness (13.64 cm), whereas the knockout plants were shorter in height (73.10 cm) and had thinner stems (10.83 cm). By integrating transcriptome sequencing technology with molecular biology methods, we aim to elucidate the molecular mechanisms underlying the role of NtPDX2 in tobacco growth and development, thereby providing new genetic resources and a theoretical foundation for the cultivation of new tobacco varieties with superior quality for flue-cured tobacco.…”
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Development and Validation of Early Alert Model for Diabetes Mellitus–Tuberculosis Comorbidity by Zhaoyang Ye, Guangliang Bai, Ling Yang, Li Zhuang, Linsheng Li, Yufeng Li, Ruizi Ni, Yajing An, Liang Wang, Wenping Gong

“…This study identified three potential immune-related biomarkers for DM–TB, and the constructed risk assessment model demonstrated significant predictive efficiency, providing an early screening strategy for DM–TB.…”
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The mitochondrial protein ORF463a mediates NWB cytoplasmic male sterility in radish through its interaction with a subunit of mitochondrial complex V by Yanping Wang, Xiangyu Wu, Yuanting Pang, Ningjie Chen, Qingbiao Wang, Li Zhang

“…Ectopic expression of orf463a with a mitochondrial transit peptide in Arabidopsis resulted in non-viable pollen and short siliques, which barely contained seeds, providing further verification of its function in inducing male sterility. …”
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The Discovery of Phages in the Substantia Nigra and Its Implication for Parkinson’s Disease by Yun Zhao, Changxian Xiong, Bingwei Wang, Daotong Li, Jiarui Liu, Shizhang Wei, Yujia Hou, Yuan Zhou, Ruimao Zheng

“…Recent discovery of virobiota reveals that viruses coexist with humans as symbionts. Newly developed transcriptomic sequencing and novel bioinformatic approaches for mining the encrypted virome in human transcriptome make it possible to study the relationship between symbiotic viruses and PD. …”
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Stereo-seq of the prefrontal cortex in aging and Alzheimer’s disease by Yun Gong, Mohammad Haeri, Xiao Zhang, Yisu Li, Anqi Liu, Di Wu, Qilei Zhang, S. Michal Jazwinski, Xiang Zhou, Xiaoying Wang, Kai Zhang, Lindong Jiang, Yi-Ping Chen, Xiaoxin Yan, Russell H. Swerdlow, Hui Shen, Hong-Wen Deng

“…In summary, this spatial transcriptome atlas provides valuable insight into AD’s molecular mechanisms.…”
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eQTL and multi-omics integration reveal PPIH as a prognostic and immunotherapeutic biomarker by Fenglin Lv, Fenglin Lv, Xinlu Zhang, Yanmei Wu, Zhipeng Li, Zhipeng Li, Xiaomen Zheng, Xiaomen Zheng, Huaxin Zhou, Huaxin Zhou, Wei Wang

“…In addition, single-cell transcriptome data were analyzed to explore the gene’s functional roles in the TME. …”
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Comprehensive analysis of long non-coding RNA and mRNA expression patterns during seminiferous tubules maturation in Guanzhong dairy goats by Fa Ren, Que Zhang, Yu Jiang, Wenling Xie, Pengyun Qiao, Jianhong Hu

“…Conclusions These findings highlight the essential roles of specific genes in the maturation of seminiferous tubules in dairy goats. This study provides comprehensive transcriptomic profiles and lncRNA-mRNA interaction networks between the G45 and G240 stages, offering valuable insights into spermatogenesis and seminiferous tubules development in Guanzhong dairy goats.…”
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Targeting CXCL8 signaling sensitizes HNSCC to anlotinib by reducing tumor-associated macrophage-derived CLU by Xin Hu, Yikang Ji, Mi Zhang, Zhihui Li, Xinhua Pan, Zhen Zhang, Xu Wang

“…Conclusion Our results provide novel evidence of a feedback loop between cancer cells and TAMs in glucose-deficient regions. …”
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The development of a high-plex spatial proteomic methodology for the characterisation of the head and neck tumour microenvironment by Chin Wee Tan, Naomi Berrell, Meg L. Donovan, James Monkman, Clara Lawler, Habib Sadeghirad, Vahid Yaghoubi Naei, Jinjin Chen, Alyssa Rossenbloom, Shilah Bonnett, Mark Conner, Brian Filanoski, Christine Kang, Joseph M. Beechem, John F. Fraser, Catherine Barnett, Rahul Ladwa, Brett G. M. Hughes, Arutha Kulasinghe

“…Taken together, this study provides a systematic workflow for the discovery and validation of high-plex protein and transcriptomic profiling in mucosal HNSCC.…”
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Droplet-based high-throughput single microbe RNA sequencing by smRandom-seq by Ziye Xu, Yuting Wang, Kuanwei Sheng, Raoul Rosenthal, Nan Liu, Xiaoting Hua, Tianyu Zhang, Jiaye Chen, Mengdi Song, Yuexiao Lv, Shunji Zhang, Yingjuan Huang, Zhaolun Wang, Ting Cao, Yifei Shen, Yan Jiang, Yunsong Yu, Yu Chen, Guoji Guo, Peng Yin, David A. Weitz, Yongcheng Wang

“…Furthermore, smRandom-seq successfully captured transcriptome changes of thousands of individual E. coli and discovered a few antibiotic resistant subpopulations displaying distinct gene expression patterns of SOS response and metabolic pathways in E. coli population upon antibiotic stress. smRandom-seq provides a high-throughput single-microbe transcriptome profiling tool that will facilitate future discoveries in microbial resistance, persistence, microbe-host interaction, and microbiome research.…”
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Transcriptional signatures of human peripheral blood mononuclear cells can identify the risk of tuberculosis progression from latent infection among individuals with silicosis by Qiao-ling Ruan, Qing-luan Yang, Yi-xin Gao, Jing Wu, Si-ran Lin, Jing-Yu Zhou, Ling-yun Shao, Sen Wang, Qian-qian Liu, Yan Gao, Ning Jiang, Wen-hong Zhang

“…Therefore, the PBMC transcriptome profiles analyzed in this study may help identify LTBI individuals who are at risk of progressing to active TB among silicosis patients and may provide new insights for targeted intervention to prevent disease progression.Clinical trial registration www.clinicaltrials.gov (NCT02430259)…”
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Feeding specialization shapes the bottom‐up effect of arbuscular mycorrhizal fungi across a plant–aphid–parasitoid system by Pasquale Cascone, Luigi Iodice, Liberata Gualtieri, Assunta Russo, Patrizia Cesaro, Zekun Yang, Michelina Ruocco, Maurilia Maria Monti, Nadia Massa, Guido Lingua, Emilio Guerrieri

“…This research has been conducted also considering the insects' feeding strategy and their feeding specialization. This study provides novel perspectives on how these fungi shape interactions in the natural world, offering potential insights for the development of sustainable pest management strategies in agriculture. …”
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Mycobacterium abscessus persistence in the face of Pseudomonas aeruginosa antagonism by Rashmi Gupta, Martin Schuster, Kyle H. Rohde

“…A tailored Mab response to Pa was evident by enhanced transcript levels of genes predicted to counteract alkylquinolone QS signals, respiratory toxins, and hydrogen cyanide.DiscussionThe study showed Mab is capable of coexisting with Pa despite Pa’s antagonistic effects, eliciting an adaptive molecular response in Mab. This study provides the first transcriptome-level insight into genetic interactions between the two CF pathogens offering potential strategies for disrupting their communities in a CF lung to improve patient clinical performance. …”
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Systematic screening of metabolic pathways to identify two breast cancer subtypes with divergent immune characteristics by Xiangshu Cheng, Shuhao Zhang, Xin Meng, Rui Chen, Hao Tang, Jiacheng Wang, Yongshuai Jiang, Ruijie Zhang

“…Nine independent bulk transcriptome cohorts (total n > 4000), including METABRIC and GEO datasets, were used for validation via random forest classification. …”
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Identification of NPR2 gene mutations affecting chondrocyte differentiation in short stature through JAK2-STAT5 by Shuoshuo Wei, Mingming He, Chuanpeng Zhang, Yanying Li, Mei Zhang, Xinguo Hou, Bo Ban, Qianqian Zhao

“…Conclusion Our study provides new insights into the loss of function of NPR2. …”
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DEPDC1B, CDCA2, APOBEC3B, and TYMS are potential hub genes and therapeutic targets for diagnosing dialysis patients with heart failure by Wenwu Tang, Wenwu Tang, Zhixin Wang, Xinzhu Yuan, Liping Chen, Haiyang Guo, Zhirui Qi, Ying Zhang, Xisheng Xie

“…Finally, qRT-PCR experiments showed that the expression trend of biomarkers was consistent with the results of transcriptome sequencing.DiscussionOverall, our results reveal the molecular mechanism of HF in patients with MHD and provide insights into potential diagnostic markers and therapeutic targets.…”
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Mechanistic study of CTHRC1 in promoting Wilms’ tumor progression by regulating M2-type tumor-associated macrophages polarization by Yingquan Zhuo, Xiaoyun Feng, Wenqi Zhang, Jun Du, Xu Sun, Xi Luo, Wei Wang, Hua Jiang, Huajian Gu

“…M2-TAMs, in turn, enhance WT malignancy by activating the PI3K/Akt pathway in tumor cells. These findings provide new potential biomarkers and therapeutic targets for WT diagnosis and treatment.…”
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Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunction by Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Cao

“…The identified key regulatory lncRNAs, miRNAs, and drugs provide potential therapeutic targets for POCD prevention and treatment.…”
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Morphological and Physiological Responses of Tomatoes to Weak Light under Artificial Climate Chamber Conditions by Weijie XU, Yingxue HU, Chao GONG, Baojuan SUN, Zhou HENG, Tao LI

“…【Objective】The study was conducted to explore the effects of weak light on tomato plant growth and physiological indicators, screen weak light tolerant materials, with a view to providing a basis for cultivating weak light tolerant tomato varieties.…”
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