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PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System
Published 2012-01-01“…For the site-specific labeling and modification of RNA by genetic alphabet expansion, we developed a PCR and transcription system using two hydrophobic unnatural base pairs: 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) and 2-nitro-4-propynylpyrrole (Px) as a third pair for PCR amplification and Ds and pyrrole-2-carbaldehyde (Pa) for the incorporation of functional components as modified Pa bases into RNA by T7 transcription. …”
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GENETIC AND MOLECULAR ANALYSIS OF COMPLEMENTATION OF THE FLAMENCO AND PIWI LOCI IN DROSOPHILA MELANOGASTER
Published 2015-01-01“…Genetic (hybridological) and molecular analysis of the interaction of the flamenco and piwi loci has been conducted. …”
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Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion
Published 2025-01-01“…Smads and their transcription factor partners mediate the transcriptional responses of target cells to secreted ligands of the transforming growth factor-β (TGF-β) family, including those of the conserved bone morphogenetic protein (BMP) family, yet only a small number of direct target genes have been well characterized. …”
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Genetic Variations and mRNA Expression of NRF2 in Parkinson’s Disease
Published 2017-01-01“…Nuclear factor erythroid 2-like 2 (NRF2) encodes a transcription factor regulating mechanisms of cellular protection and is activated by oxidative stress. …”
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Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation
Published 2020-01-01“…However, mechanistic studies employing hMSC have been hampered by the difficulty of deriving genetically modified cell lines due to the low and unstable transfection efficiency. …”
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Possible role of ARF and AP2 transcription factors in marine and terrestrial evolutionary adaptation of Rhizophoraceae plants unveiled by transcriptomic analysis
Published 2024-12-01“…The findings suggested that hormone transduction may contribute to viviparous initiation. Transcriptional factors were quite crucial for mangrove adaptation to wetlands.…”
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Pan-cancer analysis of the transcriptional expression of histone acetylation enzymes in solid tumors defines a new classification scheme for gliomas
Published 2025-01-01“…Additional glioma-specific datasets (Chinese Glioma Genome Atlas, GlioVis, GSE43378, and GSE182109) were also collected to validate the transcriptional characteristics of HATs and HDACs in gliomas. …”
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The ultradian rhythm of glucocorticoid secretion and the time course of target gene regulation
Published 2015-07-01Get full text
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Functional Genetic Variant in ATG5 Gene Promoter in Acute Myocardial Infarction
Published 2020-01-01“…In contrast, the genetic variants either identified only in controls or found in both AMI patients and controls did not affect the transcriptional activity of the ATG5 gene promoter (P>0.05). …”
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