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581
Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples
Published 2024-10-01“…Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. …”
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582
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis
Published 2020-01-01“…Mononuclear cells were stimulated with soluble Leishmania antigen (SLA) on day 0 and day 15 of therapy, and cytokine levels were determined in supernatants by ELISA. The lymphocyte proliferation and oxidative burst were evaluated by flow cytometry, and the degree of infection and Leishmania killing by optical microscopy. …”
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583
Ethanol Disrupts the Protective Crosstalk Between Macrophages and HBV-Infected Hepatocytes
Published 2025-01-01“…We found that exposure of HepG2.2.15 cells to an acetaldehyde-generating system (AGS) increased HBV RNA, HBV DNA, and cccDNA expressions and suppressed the activation of ISGs, <i>APOBEC3G</i>, <i>ISG15</i>, and <i>OAS1</i>. Supernatants collected from IFNα-activated MDMs decreased HBV marker levels and induced ISG activation in AGS-treated and untreated HepG2.215 cells. …”
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584
Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments
Published 2014-01-01“…Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer. …”
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585
Interaction of an Outflow with Surrounding Gaseous Clouds as the Origin of Late-time Radio Flares in Tidal Disruption Events
Published 2025-01-01“…Close encounter between a star and a supermassive black hole (SMBH) results in the tidal disruption of the star, known as a tidal disruption event (TDE). …”
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586
15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells
Published 2016-01-01“…In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. …”
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587
Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes
Published 2015-01-01“…Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-α, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-α, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. …”
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588
Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells
Published 2000-01-01“…Interleukin-1g (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. …”
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589
Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold
Published 2016-01-01“…Scanning electron microscopy, a cell proliferation assay, and supernatant detection indicated that the nHAC/PLA provided a suitable environment for aBMSCs. …”
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590
Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations
Published 2011-01-01“…Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNFα levels were determined by bioassay using WEHI 164 cells. …”
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591
Pancreatic Acinar Cells Employ miRNAs as Mediators of Intercellular Communication to Participate in the Regulation of Pancreatitis-Associated Macrophage Activation
Published 2016-01-01“…Additionally, the precipitates obtained from ultracentrifugation of the culture media that were rich in exosomes were markedly more potent in activating macrophages compared with the supernatant fraction lacking exosomes. The results indicated that the mediators carried by the exosomes played important roles in macrophage activation. …”
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592
Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages
Published 2018-01-01“…Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were detected in the supernatant of microencapsulated cells cocultured with TAMs but not in microencapsulated cells. …”
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593
Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes
Published 2013-01-01“…CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. …”
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594
Culture and Characterization of Microglia from the Adult Murine Retina
Published 2014-01-01“…Higher yield was obtained when retinal single-cell suspension was cultured at the density of 0.75×106 cells per cm2 in Dulbecco’s modified Eagle medium (DMEM)/F12 + Glutamax supplement with 20% fetal calf serum (FCS) and 20% L929 supernatant. We identified day 10 to be the optimum day of microglial isolation. …”
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595
The Blockade of IL6 Counterparts the Osmolar Stress-Induced Apoptosis in Human Conjunctival Epithelial Cells
Published 2016-01-01“…The expression of IL6 level was measured in the supernatant of each conditioned medium. Cell viability/apoptosis assay was performed using Annexin V/Propidium Iodide (PI) and Cell Counting Kit-8 (CCK-8). …”
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596
Study of the Potential Endocrine-Disrupting Effects of Phenylurea Compounds on Neurohypophysis Cells In Vitro
Published 2019-01-01“…OT and AVP hormone contents in the supernatant media were measured by radioimmunoassay. …”
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597
Bound lipase: an important form of lipase in rice bran (Oryza sativa)
Published 2023-09-01“…The relative catalytic activities of extraction supernatant and residue for pectinase group were (437.63 ± 22.54)% and (159.26 ± 2.12)%, respectively, which were significantly higher (P < 0.05) than other groups. …”
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598
Low IL-6, IL-10, and TNF-α and High IL-13 Cytokine Levels Are Associated with Severe Hepatic Fibrosis in Schistosoma mansoni Chronically Exposed Individuals
Published 2018-01-01“…Cytokines were measured in S. mansoni egg stimulated whole blood culture supernatants using the BD Cytometric Bead Array kits. …”
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599
Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection
Published 2012-01-01“…Cytokine and chemokines were measured in serum and in supernatants of PBMC cultures stimulated with the soluble adult worm (SWAP) or egg (SEA) antigens, using a sandwich ELISA. …”
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600
Colonic growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in severe ulcerative colitis
Published 2018-08-01“…Growth factor and chemokine levels in supernatant fluid after centrifugal separation of homogenate of 10 mg biopsy specimen were investigated by the protein analyzer «Bio-Plex» («Bio-Rad», USA). …”
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