Showing 581 - 600 results of 742 for search 'Supermax~', query time: 3.23s Refine Results
  1. 581

    Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples by Nurul Azizah, Tatan Kostaman, Yuli Arif Tribudi, Diana Andrianita Kusumaningrum, Suyatno Suyatno, Zultinur Muttaqin, Eko Koswara, Muhammad Gunawan

    Published 2024-10-01
    “…Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca2+ and Mg2+ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. …”
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  2. 582

    Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis by Fábio Peixoto, Maurício T. Nascimento, Rúbia Costa, Juliana Silva, Gaby Renard, Luiz Henrique Guimarães, Gerson Penna, Manoel Barral-Netto, Lucas P. Carvalho, Paulo R. L. Machado, Edgar M. Carvalho

    Published 2020-01-01
    “…Mononuclear cells were stimulated with soluble Leishmania antigen (SLA) on day 0 and day 15 of therapy, and cytokine levels were determined in supernatants by ELISA. The lymphocyte proliferation and oxidative burst were evaluated by flow cytometry, and the degree of infection and Leishmania killing by optical microscopy. …”
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  3. 583

    Ethanol Disrupts the Protective Crosstalk Between Macrophages and HBV-Infected Hepatocytes by Murali Ganesan, Anup S. Pathania, Grace Bybee, Kusum K. Kharbanda, Larisa Y. Poluektova, Natalia A. Osna

    Published 2025-01-01
    “…We found that exposure of HepG2.2.15 cells to an acetaldehyde-generating system (AGS) increased HBV RNA, HBV DNA, and cccDNA expressions and suppressed the activation of ISGs, <i>APOBEC3G</i>, <i>ISG15</i>, and <i>OAS1</i>. Supernatants collected from IFNα-activated MDMs decreased HBV marker levels and induced ISG activation in AGS-treated and untreated HepG2.215 cells. …”
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  4. 584

    Differences in the Antigens of Helicobacter pylori Strains Influence on the Innate Immune Response in the In Vitro Experiments by Miha Skvarc, Andreja Natasa Kopitar, Janko Kos, Natasa Obermajer, Bojan Tepes

    Published 2014-01-01
    “…Cathepsin X expression on THP-1 cells and concentration of cytokines in the supernatant of THP-1 cells were measured with a flow cytometer. …”
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  5. 585

    Interaction of an Outflow with Surrounding Gaseous Clouds as the Origin of Late-time Radio Flares in Tidal Disruption Events by Jialun Zhuang, Rong-Feng Shen, Guobin Mou, Wenbin Lu

    Published 2025-01-01
    “…Close encounter between a star and a supermassive black hole (SMBH) results in the tidal disruption of the star, known as a tidal disruption event (TDE). …”
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  6. 586

    15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells by Carlos Antônio Trindade-da-Silva, Carolina Fernandes Reis, Lara Vecchi, Marcelo Henrique Napimoga, Marcelo Sperandio, Bruna França Matias Colombo, Patrícia Terra Alves, Laura Sterian Ward, Carlos Ueira-Vieira, Luiz Ricardo Goulart

    Published 2016-01-01
    “…In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. …”
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  7. 587

    Interleukin-18 Increases TLR4 and Mannose Receptor Expression and Modulates Cytokine Production in Human Monocytes by Luciane Alarcão Dias-Melicio, Reginaldo Keller Fernandes, Daniela Ramos Rodrigues, Marjorie Assis Golim, Angela Maria Victoriano Campos Soares

    Published 2015-01-01
    “…Monocytes from healthy donors were stimulated or not with IL-18 for 18 h, and then the TLR2, TLR4, and MR expression and intracellular TNF-α, IL-12, and IL-10 production were assessed by flow cytometry and the levels of TNF-α, IL-12, IL-15, and IL-10 in culture supernatants were measured by ELISA. IL-18 treatment was able to increase TLR4 and MR expression by monocytes. …”
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  8. 588

    Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells by Juan E. Losa Garcia, María Rosa Martín de Cabo, Fernando Mateos Rodríguez, Jesús Pérez Losada, Antonio Jiménez López, José Luis Pérez Arellano

    Published 2000-01-01
    “…Interleukin-1g (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. …”
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  9. 589

    Restoration of a Critical Mandibular Bone Defect Using Human Alveolar Bone-Derived Stem Cells and Porous Nano-HA/Collagen/PLA Scaffold by Xing Wang, Helin Xing, Guilan Zhang, Xia Wu, Xuan Zou, Lin Feng, Dongsheng Wang, Meng Li, Jing Zhao, Jianwei Du, Yan Lv, Lingling E, Hongchen Liu

    Published 2016-01-01
    “…Scanning electron microscopy, a cell proliferation assay, and supernatant detection indicated that the nHAC/PLA provided a suitable environment for aBMSCs. …”
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  10. 590

    Deviations in Circulating TNFα Levels and TNFα Production by Mononuclear Cells in Healthy Human Populations by T. Mózes, I. Baráth, K. Gornicsar, A. Grosz, Ta. Mózes, Cs. Göndöcs, P. Széphalmi, K. Gaál, E. Madarász

    Published 2011-01-01
    “…Blood samples were taken; lympho/monocytes were separated and cultured with or without endotoxin (LPS) stimulation. Serum and culture supernatant TNFα levels were determined by bioassay using WEHI 164 cells. …”
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  11. 591

    Pancreatic Acinar Cells Employ miRNAs as Mediators of Intercellular Communication to Participate in the Regulation of Pancreatitis-Associated Macrophage Activation by Yong Zhao, Hao Wang, Ming Lu, Xin Qiao, Bei Sun, Weihui Zhang, Dongbo Xue

    Published 2016-01-01
    “…Additionally, the precipitates obtained from ultracentrifugation of the culture media that were rich in exosomes were markedly more potent in activating macrophages compared with the supernatant fraction lacking exosomes. The results indicated that the mediators carried by the exosomes played important roles in macrophage activation. …”
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  12. 592

    Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages by Jin-Ming Zhu, Xiu-Lian Quan, Shi-Chao Han, Xue-Jun Fan, He-Ming Li, Shan-Shan Liang, Xi Chen, Ruo-Yu Wang, Xue-Ning Ji

    Published 2018-01-01
    “…Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions were detected in the supernatant of microencapsulated cells cocultured with TAMs but not in microencapsulated cells. …”
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  13. 593

    Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes by Julia Platas, Maria Isabel Guillén, María Dolores Pérez del Caz, Francisco Gomar, Vicente Mirabet, Maria José Alcaraz

    Published 2013-01-01
    “…CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. …”
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  14. 594

    Culture and Characterization of Microglia from the Adult Murine Retina by Gayathri Devarajan, Mei Chen, Elizabeth Muckersie, Heping Xu

    Published 2014-01-01
    “…Higher yield was obtained when retinal single-cell suspension was cultured at the density of 0.75×106 cells per cm2 in Dulbecco’s modified Eagle medium (DMEM)/F12 + Glutamax supplement with 20% fetal calf serum (FCS) and 20% L929 supernatant. We identified day 10 to be the optimum day of microglial isolation. …”
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  15. 595

    The Blockade of IL6 Counterparts the Osmolar Stress-Induced Apoptosis in Human Conjunctival Epithelial Cells by Hee-Jung Ju, Yong-Soo Byun, Jee-Won Mok, Choun-Ki Joo

    Published 2016-01-01
    “…The expression of IL6 level was measured in the supernatant of each conditioned medium. Cell viability/apoptosis assay was performed using Annexin V/Propidium Iodide (PI) and Cell Counting Kit-8 (CCK-8). …”
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  16. 596
  17. 597

    Bound lipase: an important form of lipase in rice bran (Oryza sativa) by Chengwei Yu, Bin Peng, Ting Luo, Zeyuan Deng

    Published 2023-09-01
    “…The relative catalytic activities of extraction supernatant and residue for pectinase group were (437.63 ± 22.54)% and (159.26 ± 2.12)%, respectively, which were significantly higher (P < 0.05) than other groups. …”
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  18. 598
  19. 599

    Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection by Robson Da Paixão De Souza, Luciana Santos Cardoso, Giuseppe Tittoni Varela Lopes, Maria Cecília F. Almeida, Ricardo Riccio Oliveira, Leda Maria Alcântara, Edgar M. Carvalho, Maria Ilma Araujo

    Published 2012-01-01
    “…Cytokine and chemokines were measured in serum and in supernatants of PBMC cultures stimulated with the soluble adult worm (SWAP) or egg (SEA) antigens, using a sandwich ELISA. …”
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  20. 600

    Colonic growth factors (G-CSF, GM-CSF) and chemokines (MCP-1, MIP-1β) in severe ulcerative colitis by Yevgeny A. Konovich, K. Ye. Shirokikh, M. V. Shapina, Igor L. Khalif

    Published 2018-08-01
    “…Growth factor and chemokine levels in supernatant fluid after centrifugal separation of homogenate of 10 mg biopsy specimen were investigated by the protein analyzer «Bio-Plex» («Bio-Rad», USA). …”
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