Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection by Vicente P. Martins, Carina S. Pinheiro, Barbara C. P. Figueiredo, Natan R. G. Assis, Suellen B. Morais, Marcelo V. Caliari, Vasco Azevedo, William Castro-Borges, R. Alan Wilson, Sergio C. Oliveira

“…Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. …”
Get full text

Decreased Expression of CD14 in MSU-Mediated Inflammation May Be Associated with Spontaneous Remission of Acute Gout by Lihua Duan, Jiao Luo, Qiang Fu, Ke Shang, Yingying Wei, Youlian Wang, Yan Li, Jie Chen

“…The results showed that CD14 expression on macrophage and sCD14 levels in the culture supernatants were significantly decreased after MSU treatment. …”
Get full text

Protective and immunomodulatory effects of the novel probiotic yeast Pichia kudriavzevii isolated from a home-made kefir during infection in human colon epithelial cells: An explor... by Angela Maione, Marianna Imparato, Annalisa Buonanno, Marilena Galdiero, Elisabetta de Alteriis, Marco Guida, Emilia Galdiero

“…In this study we aimed to explore the protective effects of a probiotic yeast Pichia kudriavzevii (Y1) and its cell-free supernatant (CFS-Y1) during infection simulated in vitro using Caco-2 cells. …”
Get full text

Moisture Susceptibility of Warm Mix Asphalt (WMA) with an Organic Wax Additive Based on X-Ray Computed Tomography (CT) Technology by Jie Ji, Hui Yao, Zhikai Yuan, Zhi Suo, Ying Xu, Pengfei Li, Zhanping You

“…The warm mix asphalt was fabricated with different moisture contents (0%, 1%, 2%, and 3%) of limestone aggregates using the Superpave gyratory compactor. The moisture susceptibility of asphalt mixtures with an organic wax additive RH was studied. …”
Get full text

Effect of In Vitro Exposure of Corticosteroid Drugs, Conventionally Used in AMD Treatment, on Mesenchymal Stem Cells by Raffaele Nuzzi, Monica Gunetti, Deborah Rustichelli, Barbara Roagna, Francesca Fronticelli Bardelli, Franca Fagioli, Ivana Ferrero

“…Morphology, viability, growth kinetics, and immunophenotype were evaluated on healthy donors’ MSCs, treated with triamcinolone acetonide, alcohol-free triamcinolone acetonide, micronized intravitreal triamcinolone and dexamethasone at different concentrations, and in a human retinal pigment epithelial cell line supernatant (ARPE-19). The morphological analysis of MSCs in their standard medium showed a negative correlation with drug concentrations, due to the numerous crystals. …”
Get full text

Effect of Gamma-Irradiated Recycled Low-Density Polyethylene on the High- and Low-Temperature Properties of Bitumen by Perviz Ahmedzade, Alexander Fainleib, Taylan Günay, Olga Starostenko, Tetyana Kovalinska

“…Superior performing asphalt pavements (Superpave) specifications were used to analyze mechanical test results as well as to determine the performance grades (PG) of the binders. …”
Get full text

Berberine Protects against Palmitate-Induced Endothelial Dysfunction: Involvements of Upregulation of AMPK and eNOS and Downregulation of NOX4 by Ming Zhang, Chun-Mei Wang, Jing Li, Zhao-Jie Meng, Sheng-Nan Wei, Ji Li, Richard Bucala, Yu-Lin Li, Li Chen

“…Nitric oxide (NO) level and production of reactive oxygen species (ROS) were determined in supernatants or in the cultured HUVECs. The mRNA level of endothelial nitric oxide synthase (eNOS) was measured by RT-PCR, and the protein levels of eNOS, p-eNOS, Akt, p-Akt, AMPK, p-AMPK, and NADPH oxidase (NOX4) were analyzed. …”
Get full text

Exploring the antimicrobial potential of two haloarchaeal strains belonging to the genera Halopiger and Natrialba isolated from the Algerian Sahara by Inès Quadri, Imene Ikram Hassani, Hocine Hacène

“…Producing extracellular compounds associated with halocin, in the cell-free supernatant (CFS). These compounds remained stable at different temperatures (4°C, 60°C, 80°C, and 100°C) and different pH ranges (4-10 and 5-11), with antimicrobial profiles changed in response to UV light. …”
Get full text

Biocontrol of gray spot disease on Polygonatum cyrtonema Hua by Bacillus velezensis GX1 by L. Sun, G.L. Deng, H.L. Wu, Q. He, S.H. Hu, J.Y. Li, X.Y. Li, S. Meng, A.N. He, Y.Q. Tian, X. Chen, S.H. Li

“…Notably, a 10 % (v/v) concentration of cell-free supernatant (CFS) of GX1 fully inhibited mycelial growth. …”
Get full text

Multiwavelength Variability Analysis of the Blazar PKS 0727-11: An ~ 168 day Quasiperiodic Oscillation in the γ-Ray by Yuncai Shen, Tingfeng Yi, Vinit Dhiman, Lisheng Mao, Liang Dong

“…We demonstrate that QPO arising from the non-ballistic helical jet motion driven by the orbital motion in a supermassive binary black hole is a plausible physical explanation. …”
Get full text

IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways by Su-nan Li, Wei Wang, Shou-peng Fu, Jian-fa Wang, Hong-mei Liu, Shan-shan Xie, Bing-run Liu, Yang Li, Qing-kang Lv, Zhi-qiang Li, Wen-jing Xue, Bing-xu Huang, Wei Chen, Ju-xiong Liu

“…ELISA analysis showed that IL-21 also suppressed LPS-induced production of TNF-α and IL-6 in culture supernatants. Western blot analysis showed that IL-21 clearly inhibited ERK and IκBα phosphorylation and NF-κB translocation in LPS-stimulated macrophages, but it increased STAT3 phosphorylation. …”
Get full text

NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors by Jiaxin Qin, Ziqi Wang, Cheng Qian, Guohui Ji, Yizhuo Zhang, Zhanglei Cao, Bing Yan, Jun Cai

“…In addition, NupR regulates the expression of the PlcR regulon, including hemolysin, phospholipase C, <i>papR</i>, and oligopeptide permease, which could result in the culture supernatant of BMB171 being less virulent to sf9 cells compared to the <i>nupR</i> knockout strain. …”
Get full text

Parasitic Contamination of Commonly Consumed Fresh Leafy Vegetables in Benha, Egypt by Maysa Ahmad Eraky, Samia Mostafa Rashed, Mona El-Sayed Nasr, Azza Mohammed Salah El-Hamshary, Amera Salah El-Ghannam

“…Samples were washed in saline, and the resulting washing solution was filtered and centrifuged to concentrate the parasitic stages. Sediments and supernatants were examined by iodine and modified Ziehl-Neelsen stained smears. …”
Get full text

Activating PKC-ε induces HIV expression with improved tolerability. by Alivelu M Irrinki, Jasmine Kaur, Bally Randhawa, Ryan McFadden, Chelsea Snyder, Hoa Truong, Daniel Soohoo, Eric Hu, Helen Yu, Bernard P Murray, Bing Lu, Dmytro Kornyeyev, Ishak Darryl Irwan, Lan Nguyen, Yu-San Yang, Jean-Philippe Belzile, Uli Schmitz, Todd C Appleby, Brian Schultz, Jay Lalezari, Steven Deeks, Tomas Cihlar, Jeffrey P Murry

“…C-233 increased both supernatant HIV RNA and p24 expression ex vivo after treatment of CD4+ T cells from ART-suppressed people with HIV. …”
Get full text

Intrauterine Growth Restriction: Cytokine Profiles of Trophoblast Antigen-Stimulated Maternal Lymphocytes by Raj Raghupathy, Majedah Al-Azemi, Fawaz Azizieh

“…Peripheral blood mononuclear cells were stimulated with trophoblast antigens and levels of the proinflammatory cytokines IL-6, IL-8, IL-12, IL-23, IFNγ, and TNFα and the anti-inflammatory cytokines IL-4, IL-10, and IL-13 were measured in culture supernatants by ELISA. IL-8 was produced at higher levels by blood cells of the IUGR group than normal pregnant women, while IL-13 was produced at lower levels. …”
Get full text

Simultaneous Determination of Nine Neonicotinoid Insecticides and Five Metabolites in Meat by QuEChERS Coupled with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectro... by LIN Binbin, YANG Zhijin, HUANG Xuliang, LIN Xiaolian

“…After salting-out with 1 g of NaCl and 4 g of anhydrous MgSO4, the supernatant was cleaned-up using C18. The separation of the analytes was carried out on a XSelect HSS T3 (150 mm × 3.0 mm, 2.5 μm) column by gradient elution with a mobile phase composed of 0.1% formic acid and acetonitrile. …”
Get full text

Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01, a Strain Isolated from the Intestinal Tract of Infants by PENG Yunyan, ZHONG Shuying, LIU Dongmei, XU Xilin

“…At the highest concentration of 10% (V/V) in the fermentation supernatant of DMEM medium, strain B2-01 increased the survival rates of L02 cells against hydrogen peroxide damage and alcohol damage to 89.10% and 91.38%, respectively, while significantly reducing the transaminase activity in hepatocytes. …”
Get full text

Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids by Lu Zhu, Wenhao Yang, Jiaxin Luo, Danli Lu, Yanan Hu, Rui Zhang, Yan Li, Li Qiu, Zelian Chen, Lina Chen, Hanmin Liu

“…RSV infection revealed more productive replication in NO, with a downregulated immune pathway identified by RNA sequencing analysis and upregulated levels of pro-inflammatory cytokines in culture supernatants in NO compared to BO. Conclusions NO and BO serve as robust in vitro models, faithfully recapitulating the biological characteristics of upper respiratory epithelial cells. …”
Get full text

Effect of Antifungal-Treated Host Macrophages on Candida glabrata by Hong-Bin Li, Na Li, Shu-Ran Wen, Ming-Yue Qiang, Zheng-Hui Yang, Tian-Xiang Dong, Yu-Ye Li, Yi-Qun Kuang

“…Cytokines from infected macrophage supernatants and the levels of superoxide dismutase (SOD) in macrophages were measured at different time points after phagocytosis. …”
Get full text

Lactobacillus fermentum and Lactobacillus crispatus Do Not Have Cytotoxic Effects on HN5 Oral Squamous Cell Carcinoma Cell Line by Sepideh Mokhtari, Saede Atarbashi-Moghadam, Elahe Motevaseli, Soudeh Ghafouri-Fard, Ardeshir Hesampour

“…The effects of the supernatant of two lactobacilli, namely, fermentum and crispatus were studied on HN5-cancer cells. …”
Get full text