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Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes
Published 2012-01-01“…IVC establishes the link between genetic information (genotype) and the protein translated from the information (phenotype), which is essential for all directed evolution methods, by encapsulating both in a nonliving microcompartment. Herein, we introduce a new liposome-based IVC system consisting of a liposome, the protein synthesis using recombinant elements (PURE) system and a fluorescence-activated cell sorter (FACS) used as a microcompartment, in vitro protein synthesis system, and high-throughput screen, respectively. …”
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82
Investigation on mussel periostracum, a viscoelastic-to-poro-gel graded material, as an interface between soft tissue and rigid materials
Published 2024-04-01“…Abstract Mussel periostracum, a nonliving multifunctional gel that covers the rigid inorganic shells of mussels, provides protection against mechanical impacts, biofouling, and corrosion in harsh ocean environments. …”
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Primary Hepatic Lymphoma: A Challenging Diagnosis
Published 2014-01-01“…In order to define the condition as PHL, liver has to be the only site of lymphoma occurrence or to be involved in a major degree with minimal nonliver disease. Most PHLs are of B-cell origin with large cells as the main cell type.…”
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85
SUSTAINING LIFE WITH TREES: ECOCRITICISM PERSPECTIVE IN SELECTED PICTURE BOOKS
Published 2021-10-01“…Hence, taking care of the trees can be perceived as maintaining a sustainable life for both the living and nonliving things. The underlying theories to support the discussion are those of ecocriticism, narrative and illustrative elements, and the trees as metaphor of Mother Earth, all of which are interconnected in children’s picture books. …”
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86
Liver Cirrhosis: A Seven Year Follow-Up
Published 1991-01-01“…The principal cause of death was liver failure (40%), followed by nonliver causes (32.3%) and gastrointestinal bleeding (13.9%). …”
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87
Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress
Published 2020-01-01“…Abiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. …”
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88
Anti-Biofilm Effect of Ampicillin-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles Conjugated with Lysostaphin on Methicillin-Resistant Staphylococcus aureus
Published 2023-01-01“…Staphylococcus aureus exhibits the capacity to develop biofilms on various surfaces, encompassing both living and nonliving substrates, enabling it to develop resistance against the immune system and antibiotics. …”
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89
Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor
Published 2023-01-01“…In PHH from donor 2, DEX treatment inhibited protein expression and secretion of CXCL1 and CXCL8 induced by TNFα and/or LPS, whereas CXCL2 upregulation was largely unaffected by DEX treatment. In nonliver HEK293T cells GR activity inhibited CXCL2 promoter activity. …”
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