Galactin-8 DNA methylation mediates macrophage autophagy through the MAPK/mTOR pathway to alleviate atherosclerosis by Bing Xia, Yan-lin Lu, Jin Peng, Jing-wei Liang, Fang-qin Li, Jiu-yang Ding, Chang-wu Wan, Cui-yun Le, Jia-lin Dai, Jie-Wang, Bing Guo, Jiang Huang

“…The level of DNMT1 was detected by Western blot; Oil red O staining was used to detect lipid deposition in foam cells in each group, and the localization and expression levels of GAL8, MAPK/mTOR pathway proteins, LC3, Beclin1, p62, and TNF-α were quantitatively determined by Western blot. Immunofluorescence (IF) was used to detect the fluorescence intensity of GAL8, MAPK/mTOR pathway protein, LC3, p62, TNF-α, and other proteins. …”
Get full text

Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect α-SMA and Col-I mRNA levels in lung tissue. …”
Get full text

Accurate space-based NO_<i>x</i> emission estimates with the flux divergence approach require fine-scale model information on local oxidation chemistry and profile shapes by F. Cifuentes, F. Cifuentes, H. Eskes, E. Dammers, E. Dammers, C. Bryan, F. Boersma, F. Boersma

“…We conclude that accurate quantitative <span class="inline-formula">NO_<i>x</i></span> emissions estimates are possible with the FDA, but they require sophisticated, fine-scale corrections for both the <span class="inline-formula">NO₂</span> observations driving the method and the estimates of the <span class="inline-formula">NO₂</span> chemical lifetime and <span class="inline-formula">NO_<i>x</i>:NO₂</span> ratio. …”
Get full text

Exploring the role of methylation-driven gene IFFO1 in pancreatic adenocarcinoma diagnosis, prognosis and cellular functions by XU Ziqi, HU Ruizhi, LI Junjian, WANG Hongxia, SANG Youzhou

“…Western blot and real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR) were employed to assess the expression variations of IFFO1 between the normal pancreatic ductal epithelial cell line H6C7 and the PAAD cell lines MIA PaCa2, BxPC-3, AsPC-1 and Capan-2. …”
Get full text

Student Engagement and Feedback on Online Learning and Facilitation: A Case Study of the National Open University of Nigeria by Adewale Adesina, Dorothy Ofoha, Olufemi A. Peters, Isaac S. Butswat

“…Des statistiques descriptives ont été utilisées pour analyser les données quantitatives, tandis qu'une approche d'analyse thématique a été appliquée pour examiner les réponses aux questions ouvertes de l'enquête. …”
Get full text

超高效液相色谱-三重四极杆串联质谱法同时 测定植物油中4种维生素E异构体Simultaneous determination of four vitamin E isomers in vegetable oil by ultra-high performance liquid chromatography- triple quadrupole... by 李彩玲1，张见1,2，刘元法1,3，王晓超1，梅东旭1 LI Cailing1, ZHANG Jian1,2, LIU Yuanfa1,3, WANG Xiaochao1, MEI Dongxu1

“…Under these conditions, there were a good linear relationships for four vitamin E isomers in the mass concentration range of 1-100 ng/mL, with correlation coefficients (R2) above 0.998, detection limits of 6 ng/g, and quantification limits of 20 ng/g. The recovery rate ranged from 92.0% to 108.0%, the relative standard deviation was 0.7%-7.1%, and the relative error of the determination results with national standard method was -4.3%-5.1%. …”
Get full text

Biosynthesis inhibition of miR-142-5p in a N 6-methyladenosine-dependent manner induces neuropathic pain through CDK5/TRPV1 signaling by Jinshi Li, Yang Guo, Chen Zhu, Dongxu Wang, Yuan Li, Xiaotong Hao, Linyan Cao, Yiting Fan, Bo Fang

“…Methods To assess the RNA levels of TRPV1, CDK5, miR-142-5p, pre-miR-142, and pri-miR-142, quantitative PCR with reverse transcription (RT–qPCR) was utilized. …”
Get full text

CHARACTERISTICS OF CIRCADIAN BLOOD PRESSURE PROFILE IN PATIENTS WITH ACUTE CORONARY SYNDROME AND THEIR ASSOCIATION WITH THYROID FUNCTION by Nataliia Kuz, Tetiana Solomenchuk

“…To study thyroid function in patients enrolled, TSH and FT4 levels were determined by chemiluminescent immunoassay method on the ARCHITECT iSystem analyzer using reagent kits for the quantitative determination of TSH (ARCHITECT TSH) and FT4 (ARCHITECT Free T4). …”
Get full text

Systematic review and meta-analysis to examine intrapartum interventions, and maternal and neonatal outcomes following immersion in water during labour and waterbirth by Priscilla J Hall, Claire Feeley, Ethel Burns, Jennifer Vanderlaan

“…A secondary objective was to synthesise data relating to clinical care practices and birth settings that women experience who immerse in water and women who do not.Design Systematic review and meta-analysis.Data sources A search was conducted using CINAHL, Medline, Embase, BioMed Central and PsycINFO during March 2020 and was replicated in May 2021.Eligibility criteria for selecting studies Primary quantitative studies published in 2000 or later, examining maternal or neonatal interventions and outcomes using the birthing pool for labour and/or birth.Data extraction and synthesis Full-text screening was undertaken independently against inclusion/exclusion criteria in two pairs. …”
Get full text

Effect and Mechanism of Action of Pushing Method on Rats with Skeletal Muscle Overuse Injury based on Wnt/β-catenin Signaling Pathway by DUAN Miaomiao, HUANG Bo, RUAN Lei, MU Pangpang, CHEN Yiming, PENG Liang

“…The balance beam test was used to assess the motor function of the rats after skeletal muscle injury at 24 h after the end of modeling and 24 h after the end of the intervention, respectively; hematoxylin-eosin staining (HE) method was used to observe the pathological changes of the gastrocnemius muscle; EM-1400 transmission electron microscope was used to observe the ultrastructure of the skeletal muscle fibers; and fluorescence PCR was used to detect the myogenic regulatory factor myogenic differentiation antigen (Myogenic differentiation antigen (MyoD), myogenin (MyoG) and paired box 7 (Pax7) mRNA transcript levels were detected by fluorescence quantitative PCR; MyoD, MyoG, Pax7, Wnt3a, Axin2 and β-catenin protein expression levels were detected by Western blot. …”
Get full text

Effect of Slit3 on kidney fibrosis in diabetic kidney disease rats by Huang Ming-fang, Dong Yang, Shadek-jiang Harinul, Gan Xin-yu, Shi Wen-li, Lu Chen

“…The reverse-transcription quantitative real-time polymerase chain reaction （RT-qPCR） was performed to detect mRNA levels of Slit3， α-smooth muscle actin （α-SMA）， and transforming growth factor-β1 （TGF-β1） in kidney tissues， and their protein levels were detected by Western blot. …”
Get full text

Call for Papers: Demography Prize for Young Researches 2016/2017 by IF and FRFG

“…The most recent in line is the “European Index of Intergenerational Fairness”, launched in early 2016 by the Intergenerational Foundation (IF). Designed as a quantitative measurement of how the position of young people has changed across the EU, its 13 indicators include housing costs, government debt, spending on pensions and education, participation in democracy, and access to tertiary education. …”
Get full text

Student Engagement and Feedback on Online Learning and Facilitation: A Case Study of the National Open University of Nigeria by Adewale Adesina, Dorothy Ofoha, Olufemi A. Peters, Isaac S. Butswat

“…Des statistiques descriptives ont été utilisées pour analyser les données quantitatives, tandis qu'une approche d'analyse thématique a été appliquée pour examiner les réponses aux questions ouvertes de l'enquête. …”
Get full text

Podophyllotoxin mediates hepatic toxicity via the C5a/C5aR/ROS/NLRP3 and cGMP/PKG/mTOR axis in rats based on toxicological evidence chain (TEC) concept by phosphoproteomic analysis by Chuanxin Liu, Xiaobin Huang, Jiao Kong, Xuejiao Li, Yuming Wang, Fangfang Zhang, Jiajia Duan

“…This study aims to elucidate the mechanisms of PPT-induced hepatotoxicity using tandem quality tag (TMT) based quantitative proteomics and phosphoproteomics, providing potential targets and directions for developing new therapeutic strategies to facilitate the safe and rational use of podophyllotoxin in clinical settings. …”
Get full text

Yerba mate (Ilex paraguariensis) genome provides new insights into convergent evolution of caffeine biosynthesis by Federico A Vignale, Andrea Hernandez Garcia, Carlos P Modenutti, Ezequiel J Sosa, Lucas A Defelipe, Renato Oliveira, Gisele L Nunes, Raúl M Acevedo, German F Burguener, Sebastian M Rossi, Pedro D Zapata, Dardo A Marti, Pedro Sansberro, Guilherme Oliveira, Emily M Catania, Madeline N Smith, Nicole M Dubs, Satish Nair, Todd J Barkman, Adrian G Turjanski

Get full text

Designing a causal model of factors affecting the Lars supply chain by mojtaba khalesi, mojdeh rabani, Hasan Dehghan Dahnavi, Abolfazl Sadeghian, mohammad taghi honari

“…Then the structural equation method was used to quantitatively evaluate this model. The results showed that sustainability is directly related to communication with the external environment and process and production management. …”
Get full text

Clinical effect analysis of Hufu Tongbian scraping combined with Traditional Chinese medicine iontophoresis for patients with Fontaine's stage II arteriosclerosis obliteration (虎符铜... by (武亚楠), YE lei (叶蕾), HOU Jianan (侯佳楠), SU Kun (苏坤), YU Bo (于波), BAI Jianying (白建英), LI Liang (李亮)

“…Group A was given specialist nursing, group B was given specialist nursing combined with Hufu Tongbian scraping intervention, group C was given specialist nursing combined with TCM iontophoresis intervention, group D was given specialist nursing combined with Hufu Tongbian scraping and TCM iontophoresis intervention. Symptom quantitative score and Visual Analogue Score (VAS)of pain were measured before and 15 days. after intervention. …”
Get full text

MSH-YOLOv8: Mushroom Small Object Detection Method with Scale Reconstruction and Fusion by YE Dapeng, JING Jun, ZHANG Zhide, LI Huihuang, WU Haoyu, XIE Limin

“…This indicated that the MSH-YOLOv8 method possesses strong cross-dataset generalization capability and effectively recognizes small-scale targets. In addition to quantitative improvements, qualitative assessments showed that the MSH-YOLOv8 model could handle complex scenarios involving occlusions, varying lighting conditions, and different growth stages of the crops. …”
Get full text

LGR4 is essential for maintaining β-cell homeostasis through suppression of RANK by Joanna Filipowska, Zelda Cisneros, Sneha S. Varghese, Nancy Leon-Rivera, Peng Wang, Randy Kang, Geming Lu, Yate-Ching Yuan, Hung-Ping Shih, Supriyo Bhattacharya, Sangeeta Dhawan, Adolfo Garcia-Ocaña, Nagesha Guthalu Kondegowda, Rupangi C. Vasavada

Get full text

Un outil éthique, automatisé et totalement autonome pour étudier la cognition chez les primates by Jonas Fizet, Adam Rimele, Thierry Pebayle, Jean Christophe Cassel, Christian Kelche, Helene Meunier

“…Il résulte une augmentation de la quantité de données ainsi que de leur qualité, avec notamment l’affranchissement du facteur humain. …”
Get full text