Analisis Dukungan Petugas Kesehatan Dalam Optimalisasi Competence Baby Care Pada Ibu muda Melalui Pendekatan Maternal Sensitivity Models Berbasis Keluarga (Analysis of Health Of... by diyan indriyani

“…The data were then analyzed both in quantitative and qualitative methods.Results: The findings confirmed that the most common diseases undergone by infants were respiratory infections, such as diarrhea and febrile. …”
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Predictors of recurrence of ventricular tachycardia and the effectiveness of endocardial ablation at the patients with sudden cardiac death and history of myocardial infarction by O. M. Grytsay, Ya. V. Skybchyk

“…The endpoint was the recurrence of VT at the time of the patient’s last visit. Quantitative indicators are presented as mean ± standard deviation.  …”
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Effects of flame retardant tributyl phosphate on early development of zebrafish by Yao LI, Jingying ZHU, Yao LI, Limei CHEN, Pengfei ZHU, Xinliang DING, Weijie ZHOU

“…The whole-body triiodothyronine (T3) and tetraiodothyronine (T4) levels of juvenile fish were measured by enzyme immunoassay at the end of the infection, and the expression levels of hypothalamic-pituitary-thyroid axis (HPT) and neurodevelopmental-related genes were detected by quantitative real-time PCR (q-PCR). ResultsThe heart rates of zebrafish embryos were significantly decreased in all TBP-treated groups (P<0.001), the survival rates of the 0.02 and 2 μg·L−1 TBP groups were significantly decreased (P<0.05), and the malformation rate of the 2 μg·L−1 treated group was significantly increased (P<0.05), which was mainly manifested by pericardial oedema. …”
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Health-related quality of life and health state utility value in idiopathic pulmonary fibrosis: a systematic review and meta-analysis by Guixiang Zhao, Siyuan Lei, Ya Li, Zhenzhen Feng, Jiansheng Li

“…Funnel-plot directed evaluation combined with Egger’s test quantitative evaluation was conducted to detect publication bias. …”
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Impactos ambientales en la ciudad de Manaus: una búsqueda de comprensión Ambient impacts in the city of Manaus: an understanding search by Eveline Maria Damasceno do Nascimento, Iraildes Caldas Torres

“…The technical procedures include operating a set of qualitative, quantitative, compatible and complementary instruments to obtuin, organize and systematize data in the study . …”
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EyeLiner by Yoga Advaith Veturi, MSc, Steve McNamara, OD, Scott Kinder, MS, Christopher William Clark, MS, Upasana Thakuria, MS, Benjamin Bearce, MS, Niranjan Manoharan, MD, Naresh Mandava, MD, Malik Y. Kahook, MD, Praveer Singh, PhD, Jayashree Kalpathy-Cramer, PhD

“…Results: EyeLiner effectively aligns longitudinal image pairs from FIRE, SIGF, and CORIS, as qualitatively evaluated through registration checkerboards and flicker animations. Quantitative results show that the MD decreased for this model after alignment from 321.32 to 3.74 pixels for FIRE, 9.86 to 2.03 pixels for CORIS, and 25.23 to 5.94 pixels for SIGF. …”
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MiR-4298 and lncKRTAP5-6-3 regulated Cathepsin D expression through ERK-MAPK signaling pathway in chronic UVB-damaged HaCaT cells by Xinling Chen, Feng Zhou, Yao Lin, Yue Xia, Jie Zhang, Wenyi Hou, Yu Sun, Wei Lai, Yue Zheng

“…The expression levels of miR4298 and lncKRTAP5-6-3 were quantitatively analyzed using RT-PCR, while the expression of Cathepsin D and MAPK/ERK signaling pathway proteins was detected using Western blot.ResultsAfter 7 consecutive days of UVB irradiation, the expression of miR-4298 decreased by 0.64 ± 0.06 (P &lt; 0.001) compared to the un-irradiated HaCaT cells, and the expression of the KRTAP5-6-3 decreased by 0.80 ± 0.13 (P &lt; 0.001) compared to the control group. …”
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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…Thus, we explored B. burgdorferi radiosensitivity through electron paramagnetic resonance (EPR) spectroscopy by quantitating the fraction of Mn2+ present as antioxidant Mn2+ metabolite complexes (H-Mn). …”
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Mechanism study of KCMF1 promoting proliferation and NF-κB signaling transduction in colorectal cancer cells by WU Zhibai, XU Guiqin, ZHANG Li, YANG Zhaojuan, LIU Yun, JIAO Kun, CHEN Zehong, XU Chen, ZUO You, ZHENG Ningqian, YE Zhiqian, LIU Yongzhong

“…Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR), Western blot, luciferase reporter assay and cell immunofluorescence assay were utilized to validate the alteration of the affected signaling pathway. …”
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USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer by Fang Yuan, Juan Xu, Lingmei Xuan, Chan Deng, Wei Wang, Rong Yang

“…Methods: To evaluate the expression of USP14 in ESCA tissues or cells, we used RNA-Seq, immunoblotting, co-immunoprecipitation (Co-IP), ubiquitination, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence assays in this investigation. …”
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The measurement of microplastics in surface water and their impact on histopathological structures in wading birds of district Lahore by Shaza Mehboob, Khalid Mahmood Anjum, Hamda Azmat, Muhammad Imran

“…Birds from Site I (Main Stream of River Ravi) had 100% of their organs containing plastic items compared to those from Site II (Shahdara Drain). Quantitative and qualitative histopathological analyses were performed to examine variations in prevalence percentage, frequency, and histological alteration indices (HAI) as a consequence of MPs exposure on the health of wild species. …”
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Genetic characteristics involving the PD-1/PD-L1/L2 and CD73/A2aR axes and the immunosuppressive microenvironment in DLBCL by Tingting Zhang, Zhenzhen Zhang, Xiubao Ren, Jin He, Zhengzi Qian, Bin Meng, Lei Jiao, Hengqi Liu, Lanfang Li, Lihua Qiu, Shiyong Zhou, Wenchen Gong, Huilai Zhang, Xianhuo Wang

“…In addition, multiplex immunofluorescence staining was used to quantitatively assess the expression of dysfunctional CD8+ T cells in DLBCL.Results SP140 was identified as a novel translocation partner for PD-L1, and a new inversion was detected between PD-L1 and PD-L2, both leading to the upregulation of PD-L1 expression. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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Evaluation of microRNAs as liquid biopsy markers in adrenocortical tumors by Chrysoula Mytareli, Vassiliki Kalotychou, Alexandros Lafioniatis, Gregory Kaltsas, George N. Zografos, Athina Markou, Labrini Papanastasiou, Athanasios Fountas, Dimitra Argyro Vasilliadi, Evanthia Kassi, Evanthia Kassi, Marina Mantzourani, Anna Angelousi

“…Among the 56 patients with ATs evaluated preoperatively (50 with ACAs and 6 with ACC), 26 had non-functioning tumors, 22 exhibited mild autonomous cortisol secretion, and 8 had Cushing syndrome. Quantitative real-time polymerase chain reaction was employed to analyze microRNA expression.ResultsCirculating levels of miR-483-5p and miR-210 were significantly elevated in patients with active ACC compared to both ACAs (p&lt;0.001 and p=0.004, respectively) and controls (p=0.02 and p = 0.03, respectively). …”
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Association between METTL14 gene polymorphisms and risk of ovarian endometriosis by Zijun Zhou, Zijun Zhou, Youkun Jie, Xianyue Hu, Xianyue Hu, Guange Chen, Yanjing Bao, Yanjing Bao, Zhenbo OuYang, Liangzhi Wu, Tianyang Gao, Qiushi Zhang, Qiushi Zhang, Wenfeng Hua, Wenfeng Hua

“…Additionally, Bayesian false discovery probability and false positive report probability analysis confirmed the robustness of the significant findings. Expression quantitative trait loci analysis revealed a significant association between the rs9884978 GA/AA genotypes and elevated METTL14 mRNA levels in fibroblasts and adrenal gland. …”
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Hepatitis D virus infection triggers CXCL9-11 upregulation in hepatocytes and liver infiltration of CXCR3+ CD4 T cells by Jan-Hendrik Bockmann, Lena Allweiss, Annika Volmari, David da Fonseca Araújo, Matin Kohsar, Anastasia Hyrina, Janine Kah, Zhijuan Song, Josolyn Chan, Katja Giersch, Tassilo Volz, Marc Lütgehetmann, Jeffrey J. Wallin, Dmitry Manuilov, Meghan M. Holdorf, Simon P. Fletcher, Ansgar W. Lohse, Antonio Bertoletti, Julian Schulze zur Wiesch, Maura Dandri

“…Methods: We performed quantitative PCR, RNA in situ hybridisation, activation-induced marker (AIM) assays, and FACS analysis to investigate the CXCR3/CXCL9–11 receptor/ligand axis of T cells in peripheral blood and livers from patients with chronic hepatitis B (n = 27 and 18, respectively) and CHD (n = 20 and 18, respectively). …”
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EZH2/miR-142-3p/HMGB1 axis mediates chondrocyte pyroptosis by regulating endoplasmic reticulum stress in knee osteoarthritis by Yang Chen, Shanshan Dong, Xin Zeng, Qing Xu, Mingwei Liang, Guangneng Liao, Lan Li, Bin Shen, Yanrong Lu, Haibo Si, Peifang Wei

“…The expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2), microRNA-142-3p (miR-142-3p), and high mobility group box 1 (HMGB1) and the levels of ER stress, pyroptosis, and metabolic markers in normal and OA chondrocytes were investigated by western blotting, quantitative polymerase chain reaction, immunohistochemistry, fluorescence in situ hybridization, fluorescein amidite-tyrosine-valine-alanine-aspartic acid-fluoromethyl ketone (FAM-YVAD-FMK)/Hoechst 33342/propidium iodide (PI) staining, lactate dehydrogenase (LDH) release assays, and cell viability assessments. …”
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Whole blood ratio of CDK1/CX3CR1 mRNA expression combined to lactate refines the prediction of ICU mortality in septic patients in the Sepsis-3 era: a proof-of-concept study by Marie-Angélique Cazalis, Marie-Angélique Cazalis, Louis Kreitmann, Louis Kreitmann, Guillaume Monneret, Guillaume Monneret, Alexandre Pachot, Alexandre Pachot, Karen Brengel-Pesce, Karen Brengel-Pesce, Jean-François Llitjos, Jean-François Llitjos, Jean-François Llitjos

“…We validated mRNA biomarkers using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) on an external validation cohort. …”
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Women's experiences and acceptability of self-administered, home delivered, intravaginal 5-Fluorouracil cream for cervical precancer treatment in Kenya by Konyin Adewumi, Aparna Ghosh Kachoria, Everlyn Adoyo, Mercy Rop, Mercy Rop, Antony Owaya, Jennifer H. Tang, Jennifer H. Tang, Lisa Rahangdale, Lisa Rahangdale, Lisa Rahangdale, Chemtai Mungo, Chemtai Mungo, Chemtai Mungo

“…To understand women's experiences with self-administered 5FU, we evaluated the acceptability of this intervention among trial participants.MethodsAll 12 participants from the Phase I trial completed a structured questionnaire and in-depth semi-structured interviews in their preferred language, focusing on their experiences with 5FU self-administration, challenges faced, and overall acceptability of the intervention, including whether they would use it again or recommend it to someone who needed it. Quantitative data were analyzed using descriptive statistics. …”
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PD-L1/TLR7 dual-targeting nanobody-drug conjugate mediates potent tumor regression via elevating tumor immunogenicity in a host-expressed PD-L1 bias-dependent way by Wei Huang, Ji Zhou, Jia Li, Yakun Wan, Chao Hu, Meng Liu, Pan Xu, Feng Tang, Jianhua Sun, Xiaolu Yu, Yiru Long, Binfan Chen, Yongliang Tong, Mengwen Shan, Xiaomin Jia, Henglei Lu, Runqiu Chen, Jin Ren, Guangyi Jin, Likun Gong

“…Here, we aimed to improve efficacy for anti-programmed death ligand 1 (PD-L1) therapy by developing a PD-L1/TLR7 dual-targeting nanobody-drug conjugate (NDC), based on the PD-L1 nanobodies and TLR7 agonist we developed.Methods PD-L1 nanobodies were obtained by phage display screening and identified through T-cell activation bioassay, in vivo imaging and quantitative biodistribution study. Immune activation and PD-L1-inducing of TLR7 agonists were evaluated in diverse innate cell models. …”
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