Use of Go-Beyond as a Self-Directed Internet-Based Program Supporting Veterans’ Transition to Civilian Life: Preliminary Usability Study by Karolina Katarzyna Alichniewicz, Sarah Hampton, Madeline Romaniuk, Darcy Bennett, Camila Guindalini

“…Users were then automatically allocated to Go-Beyond modules that aligned with their M-CARM profile. Additionally, quantitative and qualitative data were collected from a survey on aesthetics, interactivity, user journey, and user experience, which was optional for users to complete at the end of each module. …”
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Phytochemical and Bioactivity Evaluation of Bee Pollen and Androecia of <i>Castanea</i>, <i>Salix</i>, and <i>Quercus</i> Species by Nisa Beril Sen, Irena Vovk, Hasan Kırmızıbekmez, Etil Guzelmeric

“…Qualitative and quantitative differences in the chemical composition between bee pollen originated from <i>Castanea sativa</i> (Türkiye and Slovenia), <i>Salix</i> spp. …”
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Integrating Bulk and Single-Cell Transcriptomic Data to Identify Ferroptosis-Associated Inflammatory Gene in Alzheimer&rsquo;s Disease by Zhou H, Peng Y, Huo X, Li B, Liu H, Wang J, Zhang G

“…Finally, candidate genes were validated in ferroptosis models using reverse transcription quantitative PCR (RT-qPCR) and immunofluorescence methods.Results: Bulk RNA-seq analysis identified SLC11A1, an inflammatory gene associated with AD. …”
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Development of an Integrated Model of Planning and Management in Free Zones with an Emphasis on Environmental Pressures by Mohammad Javad Amiri, Vahid Moghaddamzadeh, Gholamreza Nabi Bidhendi

“…Data collection was conducted through both qualitative (library research) and quantitative (a researcher-designed questionnaire) methods, while data analysis utilized SPSS version 23 and Topsis Fuzzy techniques. …”
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The Role of Macroeconomic Indicators and National Zakat Index in Advancing the Islamic Human Development Index (I-HDI): A Case Study of Districts and Cities in Banten Province by Elif Pardiansyah, Mohamad Ainun Najib

“…The primary purpose is to assess how economic growth, unemployment, poverty, and the National Zakat Index influence I-HDI. A quantitative approach using Ordinary Least Squares (OLS) and multiple regression analysis was applied. …”
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Probiotic potential and safety properties of Limosilactobacillus fermentum A51 with high exopolysaccharide production by Guangqiang Wei, Daodian Wang, Daodian Wang, Teng Wang, Gao Wang, Yunmei Chai, Yufang Li, Minhui Mei, Hao Wang, Aixiang Huang

“…These probiotic genes were verified by quantitative real-time PCR. In vitro assays confirmed that L. fermentum A51 showed good tolerance to simulated gastrointestinal tract (8.49 log CFU/mL), 0.3% bile salt (39.06%), and possessed adhesion (86.92%), antioxidant (70.60–89.71%), and antimicrobial activities, as well as EPS and signaling molecule AI-2 synthesis capacities.ConclusionCollectively, our findings have confirmed that L. fermentum A51 is safe and exhibits good probiotic properties, thus recommending its potential application in the production of value-added fermented dairy products.…”
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A case-control genome-wide association study of estimated breeding values for resistance to gastrointestinal nematodes in two local dairy sheep breeds by C. Persichilli, S. Biffani, G. Senczuk, M. Di Civita, M.K. Bitew, A. Bosco, L. Rinaldi, S. Grande, G. Cringoli, F. Pilla

“…A genome-wide case-control analysis was performed retaining the most significant single nucleotide polymorphisms (SNPs) with a threshold of 0.005% for the false discovery rate. Genes and Quantitative Trait Loci overlapping significant SNPs were annotated and enriched respectively while genes have been also enriched for functional pathways. …”
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Growth of microbes in competitive lifestyles promotes increased ARGs in soil microbiota: insights based on genetic traits by Zishu Liu, Xiangwu Yao, Chengyi Chen, Yuxiang Zhao, Chifei Dong, Lingtao Sun, Junxian Zhao, Baofeng Zhang, Zhendi Yu, Dongqing Cheng, Lizhong Zhu, Baolan Hu

“…Paired with shot-gun metagenomics analysis and quantification of bacterial growth, trait-based assessment of soil microbiota was applied to reveal the association between key ARG subtypes, representative bacterial taxa, and functional-gene features that drive the growth of ARGs. …”
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Hypoxic BMSC-derived exosomal miR-210-3p promotes progression of triple-negative breast cancer cells via NFIX-Wnt/β-catenin signaling axis by Meng Wang, Yi Zheng, Qian Hao, Guochao Mao, Zhijun Dai, Zhen Zhai, Shuai Lin, Baobao Liang, Huafeng Kang, Xiaobin Ma

“…Expression levels of genes and proteins were assessed by quantitative real-time PCR and western blot. Cell proliferation, apoptosis, migration and invasion were analyzed using CCK-8 assay, EDU assay, transwell migration, wound healing assay and invasion assay, respectively. …”
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Accurate phenotype-to-genotype mapping of high-diversity yeast libraries by heat-shock-electroporation (HEEL) by Marcus Wäneskog, Emma Elise Hoch-Schneider, Shilpa Garg, Christian Kronborg Cantalapiedra, Elena Schäfer, Michael Krogh Jensen, Emil Damgaard Jensen

“…This design allows for robust identification and quantification of unique genotypes within a heterogeneous population using standard Sanger sequencing. …”
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Whole-genome resequencing reveals collagen-related genes in Kele pigs. by Yu Dan Zhang, Wei Yuan, Huan Bi, Xiao Yang, Yi Yu Zhang, Wei Chen

“…In addition, through real-time fluorescence quantitative PCR results, we found that the expression levels of collagen-related genes COL9A1 and COL6A5 in skin tissues were higher than those in other tissues, and the expression levels of COL4A4 and COL4A3 in kidney tissues were higher than those in other tissues. …”
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Overexpression of multiple cytochrome P450 genes with and without knockdown resistance mutations confers high resistance to deltamethrin in Culex quinquefasciatus by Saowanee Chamnanya, Benyapa Kiddela, Jassada Saingamsook, Woottichai Nachaiwieng, Nongkran Lumjuan, Pradya Somboon, Jintana Yanola

“…Eight cytochrome P450 genes were analyzed for the expression level using quantitative real time-PCR. The cDNA of mosquitoes was amplified and sequenced for four fragments of vgsc gene. …”
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Mycochemistry, antioxidant, anticancer activity, and molecular docking of compounds of F12 of ethyl acetate extract of Astraeus asiaticus with BcL2 and Caspase 3 by Swapan Kumar Ghosh, Koushik Pandey, Madhuparna Ghosh, Pradip Kumar Sur

“…The fruit body of edible and tasty Astraeus asiaticus mushroom was extracted in ethyl acetate solvent (EA) and quantitative analysis revealed that it contained a significant amount of total phenols, flavonoids, and ascorbic acids. …”
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Analysis of Sharia Commercial Banks in the Review of Maqashid Sharia with an Islamicity Performance Index Approach by Prima Dwi Priyatno, Agus Maulana, Dewi Darmastuti

“…The method in this study uses a descriptive quantitative method with the Islamicity Performance Index model, the data used is secondary data from the financial statements of Islamic commercial banks in Indonesia. …”
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ANALISIS PERANCANGAN STRUKTUR BETON BERTULANG UNTUK FUNGSI RENTAL OFFICE 30 LANTAI DENGAN PENEKANAN SISTEM RIGID FRAME BERKANTILEVER by Reinhart Bunadi, Nasruddin Junus, Imriyanti Imriyanti

“…The analysis aims to determine the exact structure details for the building with the SAP2000 program v.14. Research is quantitative because it determines the dimensions of the structure. …”
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Biofloc technology significantly reshapes water microbiome and improves survival rates in Japanese eel (Anguilla japonica) by Jiho Yang, Hyunjun Choi, Jun Seong Park, Yehyeon Cha, Ju-Ae Hwang, Seung-Yoon Oh

“…In this study, we observed the survival and growth rates of eels treated with BFT over 8 weeks and monitored changes in the gut and water microbiome using 16S rRNA amplicon sequencing (metabarcoding) and quantitative PCR. Our research demonstrated that BFT treatment was more advantageous for eel growth compared with FTS in terms of eel survival rate, feed intake amount, and growth rate. …”
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Novel banana lectin CAR-T cells to target pancreatic tumors and tumor-associated stroma by Malcolm K Brenner, Challice Bonifant, Norihiro Watanabe, Mary K McKenna, Daniel Brenner, Ada Ozcan, Maureen Legendre, Dafydd G Thomas, Christopher Ashwood, Richard D Cummings, David M Markovitz

“…Here, we tested the efficacy of our novel H84T CAR in models of pancreatic ductal adenocarcinoma (PDAC), intractable tumors with aberrant glycosylation and characterized by desmoplastic stroma largely contributed by pancreatic stellate cells (PSCs).Methods We transduced human T cells with a second-generation retroviral construct expressing the H84T BanLec chimeric receptor, measured T-cell expansion, characterized T-cell phenotype, and tested their efficacy against PDAC tumor cells lines by flow cytometry quantification. In three-dimensional (3D) spheroid models, we measured H84T CAR T-cell disruption of PSC architecture, and T-cell infiltration by live imaging. …”
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Mycobiome analyses of critically ill COVID-19 patients by Danielle Weaver, Sara Gago, Matteo Bassetti, Daniele Roberto Giacobbe, Juergen Prattes, Martin Hoenigl, Florian Reizine, Hélène Guegan, Jean-Pierre Gangneux, Michael John Bromley, Paul Bowyer

“…Respiratory mycobiome was profiled using internal transcribed spacer 1 sequencing, and Aspergillus fumigatus burden was further validated using quantitative PCR. Fungal communities were investigated using alpha diversity, beta diversity, taxa predominance, and taxa abundances. …”
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Effects of intermittent theta burst stimulation on cognitive and swallowing function in patients with MCI and dysphagia risk: a randomized controlled trial by Jie Wang, Mengqing Zhang, Xiaomei Wei, Cheng Yang, Meng Dai, Zulin Dou, Yonghui Wang

“…Global cognitive function was assessed using the Montreal Cognitive Assessment (MoCA), and executive function was evaluated with the Trail Making Test (TMT), Digital span test (DST) and Stroop color word test (SCWT). Quantitative swallowing measurements were obtained using temporal and kinetic parameters based on the videofluoroscopic swallowing study (VFSS). …”
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Perfluorooctanoic acid induces abnormal heart development via aryl hydrocarbon receptor-mediated oxidative stress and apoptosis in zebrafish larvae by Xueyi CHEN, Tianchi MA, Kang WANG, Tao CHEN, Yan JIANG

“…Total RNA was extracted from dissected hearts, and mRNA expression levels of oxidative stress-related genes (sod2, cat) and apoptosis-related gene (p53) were analyzed using quantitative PCR. ResultsCompared to the DMSO control group, PFOA at even the lowest concentration (1 μg·L−1) increased heart malformation rate (P＜0.05) and reduced heart rate (P＜0.01) in the zebrafish larvae at 72 hpf, and the results showed a clear concentration-response relationship. …”
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