Rh-relaxin-2 attenuates oxidative stress and neuronal apoptosis via ERK-nNOS-NO pathway after germinal matrix hemorrhage in rats by Jun Liu, Yonghua Cai, Khalil ur Rahman, Qixiong Zhou, Guangjie Liu, Huibin Kang, Mingzhou Li, Shichao Zhang, Gang Wang, Wenfeng Feng, Xi’an Zhang, Guozhong Zhang, Ye Song, Peng Li

“…Co-immunoprecipitation, immunofluorescence, TUNEL, Fluoro-Jade C, DHE staining, western blot, Nitrix Oxide (NO) quantification and side effect experiments were performed to evaluate post-GMH. …”
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Low Expression of FFAR2 in Peripheral White Blood Cells May Be a Genetic Marker for Early Diagnosis of Acute Myocardial Infarction by Jianjun Ruan, Heyu Meng, Xue Wang, Weiwei Chen, Xiaomin Tian, Fanbo Meng

“…Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of the FFAR2 gene. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Biofilm viability was assessed using CAM/TMA-DPH or SYTO9/PIstaining, followed by analysis with confocal laser scanning microscopy (CLSM) and ImageJ-based biofilm surface coverage quantification. Viability findings were compared with colony-forming units (CFU/mL), a standard microbial viability measure.ResultsCAM/TMA-DPH staining demonstrated strong positive correlations with CFU counts across all bacterial species (r = 0.59 - 0.91), accurately reflecting biofilm vitality. …”
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Semen Analysis of Covid-19 Survivors and Uninfected Men: Examining the Motility, Concentration, Vitality, and Morphology Parameters of Spermatozoa by Sri Musta'ina, Eko Budi Siswidiyanto, Pety Narulita, Agustinus, Aucky Hinting, Atika, Zakiyatul Faizah

“…Semen quality assessment using the WHO Semen Analysis Guide 6th edition. Quantitative data were analyzed using SPSS. The data normality test was carried out using the Saphiro-Wilk test. …”
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NCAPD3-mediated ferroptosis of 2,3,5,4’-Tetrahydroxystilbene-2-O-β-D-glucoside inhibits proliferation in T47D cells by Jianfen Shen, Shuo Zhang, Shuo Zhang, Yan Song, Yan Song, Leiming Yang, Leiming Yang, Qi Huang, Qi Huang, Pengyu Wang, Pengyu Wang, Youzhi Zhang, Youzhi Zhang

“…And the hub genes (transcripts) were selected using the bioinformatics analysis, quantitative polymerase chain reaction (qPCR) and Western blotting (WB). …”
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Matrine Inhibits Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition Through Regulating the LINC01116/miR-9-5p/ITGB1 Axis by Lili Ren, Ziru Fang, Jiaojiao Xu, Xiaoxiao Wu, Yongjun Zhang, Hu Cai, Zhicun Han

“…The BC cells (MDA-MB-231 and MCF-7) were treated with various concentrations of MAT, and the optimal dose and timing of MAT action were determined using CCK-8 and quantitative real-time polymerase chain reaction assays. …”
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Community health system capacities and capabilities within an evolving community health policy framework: mixed methods study of stakeholders in central Uganda by Raymond Tweheyo, Diana Atwine, Richard Kabanda, Ronald Miria Ocaatre, Bounggui Kim, Simon Erisa Waiswa, Prichard Denzel Kavuma, Yeni Lee, Loretah Mutoni, Solyi Kim, Yein Park, Monica Okuga

“…While descriptive and stratified analyses were conducted for quantitative data in Stata I/C 15.0: proportions for the varied geographical entities were compared using the t-test with p values <0.05 considered significant, one-way ANOVA was used to compare means.Results Overall, all sites had relatively strong governance of community health only challenged by multiple implementing partners that were weakly coordinated. …”
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Deployment and uptake of COVID-19 vaccines for refugees and migrants in regular and irregular situations: a mixed-method multicountry study by Biraj Man Karmacharya, Karl Blanchet, Jai K Das, Laetitia Nyirazinyoye, Maria Midea M Kabamalan, Nguyen Toan Tran, Zahra A Padhani, Anjali Joshi, Seyed-Moeen Hosseinalipour, Enrique Teran, Pierina Benavente, Vinay N Kampalath, Moussa Lonkila Zan, Elżbieta Anna Czapka, Cheryl Martens, Vicente B Jurlano

“…Both the qualitative and quantitative phases identified several barriers and facilitators. …”
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Impacts of plant root traits and microbial functional attributes on soil respiration components in the desert-oasis ecotone by Jinlong Wang, Jinlong Wang, Jinlong Wang, Guanghui Lv, Guanghui Lv, Guanghui Lv, Jianjun Yang, Jianjun Yang, Jianjun Yang, Xuemin He, Xuemin He, Xuemin He, Hengfang Wang, Hengfang Wang, Hengfang Wang, Wenjing Li, Wenjing Li, Wenjing Li

“…Employing metagenomics, we quantitatively characterized microbial taxonomic attributes (i.e., taxonomic composition) and functional attributes (specifically, functional genes implicated in microbial carbon metabolism). …”
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Health checks for autistic adults: study protocol for a cluster randomised controlled trial by Jeremy R. Parr, Helen Taylor, Colin Wilson, Clare Scarlett, Sarah Al-Asmori, Carole Buckley, Sally-Ann Cooper, Cristina Fernandez-Garcia, Tracy Finch, Rhianna Lees, Nicholas Lennox, Hannah Merrick, Sebastian Moss, Christina Nicolaidis, Malcolm Osbourne, Dora M. Raymaker, Tomos Robinson, Anna Urbanowicz, James M. S. Wason, Barry Ingham

“…Data will be collected through quantitative and qualitative methods. The primary outcome will be the incidence of new health needs/conditions detected and met at 9 months (data gathered from participant’s GP records). …”
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Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury by Luying Zhang, Song Yang, Hao Cui, Chenchen Hang, Xingsheng Wang, Le An, Zhenyu Shan, Zhen Liang, Rui Shao, Ziren Tang

“…Expression of mitophagy-related proteins (Cleaved PINK1, Parkin, SQSTM1/p62, Beclin-1, LC3B II/LC3B I), apoptosis-related proteins (Bcl-2, Cytochrome C, caspase-3, cleaved caspase3) and VDAC3 were assessed using western blot analysis and quantitative real-time PCR. The interaction between Parkin and VDAC3 was confirmed by immunofluorescence colocalization. …”
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Dual disease co-expression analysis reveals potential roles of estrogen-related genes in postmenopausal osteoporosis and Parkinson’s disease by Dongdong Yu, Dongdong Yu, Jian Kang, Chengguo Ju, Qingyan Wang, Ye Qiao, Ye Qiao, Long Qiao, Long Qiao, Dongxiang Yang

“…The study also involved constructing TF-gene interactions, TF-microRNA coregulatory networks, interactions of hub genes with diseases, and validation through quantitative reverse transcription polymerase chain reaction (qRT-PCR).ResultsThe colocalization analysis uncovered shared genetic variants between PD and osteoporosis, with a posterior probability of colocalization (PPH4) measured at 0.967. …”
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Impaired hemodynamic forces assessed by routine CMR and its determinants in different duration T2DM patients with normal LV function and myocardial strain by Guozhu Shao, Guozhu Shao, Guozhu Shao, Yukun Cao, Yukun Cao, Yukun Cao, Yue Cui, Yue Cui, Yue Cui, Hongyan Li, Heshui Shi, Heshui Shi, Heshui Shi

“…The objective of this study is to quantitatively assess hemodynamic forces (HDFs) using a rigorous mathematical model based on conventional cine cardiac magnetic resonance (CMR) images in patients with T2DM, and investigate their correlation with late gadolinium enhancement (LGE) and duration of diabetes.MethodsWe recruited 63 T2DM patients and 50 healthy volunteers to undergo contrast-enhanced CMR examinations. …”
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Pharmacological interventions for the management of anesthesia and sedation in patients with Duchenne muscular dystrophy: a systematic review and meta-analysis by Xianghong Lian, Xianghong Lian, Xianghong Lian, Yang Jing, Yang Jing, Yang Jing, Ting Luo, Ting Luo, Ting Luo, Yixin Guo, Yixin Guo, Yixin Guo, Yunzhu Lin, Yunzhu Lin, Yunzhu Lin

“…Precautions for DMD patients should include quantitative neuromuscular monitoring, electrocardiographic monitoring and rapid airway protection throughout anesthesia. …”
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Metabolic profiling of Perilla leaves of different colors: insights into metabolite variation and bioactive compound distribution by Senouwa Segla Koffi Dossou, Qianchun Deng, Feng Li, Lei Wang, Nanjun Jiang, Habtamu Kefale, Rong Zhou, Donghua Li, Meilian Tan, Linhai Wang

“…Results We qualitatively and quantitatively identified a total of 1239 metabolites in Perilla leaves of different colors. …”
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A lightweight wheat ear counting model in UAV images based on improved YOLOv8 by Ruofan Li, Xiaohua Sun, Kun Yang, Zhenxue He, Zhenxue He, Xinxin Wang, Xinxin Wang, Chao Wang, Chao Wang, Bin Wang, Fushun Wang, Fushun Wang, Hongquan Liu, Hongquan Liu

“…Wheat (Triticum aestivum L.) is one of the significant food crops in the world, and the number of wheat ears serves as a critical indicator of wheat yield. Accurate quantification of wheat ear counts is crucial for effective scientific management of wheat fields. …”
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Multi-omics dissection of metabolic dysregulation associated with immune recovery in people living with HIV-1 by Lin-Yu Wan, Sin Man Lam, Hui-Huang Huang, Wen-Jing Cao, Xiang-Yi Cao, Xue-Meng Li, Li-Ping Zhang, Jia-Min Gao, Chao Zhang, Xing Fan, Yan-Mei Jiao, Guanghou Shui, Fu-Sheng Wang, Jin-Wen Song

“…Methods In this cross-sectional study, mass spectrometry was used for quantitative analysis of plasma metabolome and lipidome in 24 treatment-naïve PLWH (TNs), 33 immunological responders (IRs), 35 immunological non-responders (INRs), and 16 healthy controls (HCs). …”
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Improving the diagnostic accuracy of referrals for papilloedema (DIPP) study: protocol for a mixed-methods study by Luke Bennetto, Beth Stuart, Jonathan Benger, Matthew J Ridd, Helen Bolton, William Hollingworth, Michael Bowen, Amar Shah, Samuel William David Merriel, Alyson Huntley, Victoria Wilson, Maria Theresa Redaniel, Paul Roy, Denize Atan, Frank Moore, Olivia Skrobot, Mary-Ann Sherratt, Marcia Lucraft, Christina Stokes

“…This article describes the protocol for gathering evidence of current referral practices and pathways for people suspected to have papilloedema in England and the development of guidelines based on this evidence and extensive engagement with community- and hospital-based healthcare professionals, patients, and the public.Methods and analysis Both qualitative and quantitative data will be collected from Freedom of Information requests to Integrated Care Boards across England about how they organise their community and hospital services for people with suspected papilloedema, with and without headache. …”
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A novel compound heterozygous mutation in the DYNC2H1 gene in a Chinese family with Jeune syndrome by Sujie Xiong, Guangyao Hu, Yao Zhou, Fei Sun, Yanlin Ma

“…Methods Cytogenetic and molecular genetic analyses of amniotic fluid via whole-genome sequencing (WGS), routine G-banded karyotype analysis, fluorescent quantitative polymerase chain reaction, and whole-exome sequencing (WES) were performed at 19 weeks. …”
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Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation by Chao Wang, Xiao-xia Hou, Hong-liang Rui, Li-jing Li, Jing Zhao, Min Yang, Li-jun Sun, Hong-rui Dong, Hong Cheng, Yi-Pu Chen

“…The expression of mRNA and protein of P2X7R and NLRP3 inflammasome (NLRP3, ASC, and caspase-1) and downstream effectors (IL-1β and IL-18), as well as podocyte-associated molecules, was determined by real-time quantitative PCR and Western blot assay, respectively. …”
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