Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis by Gu Man, Haiwen Yang, Kai Shen, Donghong Zhang, Julin Zhang, Hao Wu, Hongyu Zhang, Jinhuan Wang

“…The function of circRHOT1 in the regulation of OA was assessed by CCK-8, colony formation, flow cytometry analysis, quantitative real-time PCR, Western blot analysis, and luciferase reporter gene assay in chondrocytes. …”
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Development and validation of a UV/visible spectrophotometric method for simultaneous assay of paracetamol and ibuprofen by Timothée Mavanga Mabaya, Jocelyn Mankulu Kakumba, Mannix Mayangi Makola, Adelard Mbenza Puati, Didi Mana Kialengila, Jean Mavar Tayey Mbay, Jérémie Mbinze Kindenge

“…After evaluating all the criteria, the developed and validated method was applied for the qualitative and quantitative analysis of dosage forms containing both paracetamol and ibuprofen. …”
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Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos by Yuxin Chen, Ziwen Yang, Zhiqiang Guo, Lemei Zhan, Kenneth B. Storey, Danna Yu, Jiayong Zhang

“…The present study quantified the transcript levels of mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction (<i>RT-qPCR</i>) in samples of larvae of three dragonfly species (<i>A. parthenope</i>, <i>E. elegans</i>, and <i>G. confluens</i>) under different levels of CPF stress. …”
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Abnormal Expression of Peripheral Blood Leukocyte Surface Markers in Retinopathy of Prematurity Patients by Guo R, Cheng F, Meng XJ, Fan JM, Lu YL, Ma XH, Qiao X, Li JH

“…This method allowed for the quantification of specific cell subset proportions.Results: A total of 2,110 preterm infants were screened for inclusion in the study. …”
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Extracellular vesicles (EVs) in seminal plasma of buffalo bulls possess spermatozoa specific fertility associated proteins and enzymes by Rakesh Kumar, Mir Ahmad, Bala Subramaniam, Ankit Pal, Aditya Patel, Shiva Shiva, Vitika Chauhan, Seema Karanwal, Mukesh Bhakat, Tirtha K. Datta

“…The NTA analysis provides a quantitative abundance of EVs, and the EVs population was 1.5x109 to 2.9x1011 EVs/ml in seminal plasma. …”
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Political Marketing: Measurement Parameters of the Concept of Social Value in Possible Presidential Pre-Candidates, Elections 2021, Chimborazo, Ecuador by L. Vallejo, M. Torreso, C. Buenaño, C. Buenaño

“… This research sought to determine what parameters of social value should be met by potential presidential candidates through an analysis of political marketing. Qualitative and quantitative approaches were used, at a descriptive, observational and prospective level, with a non-experimental cross-sectional design. …”
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The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…We detected the location of the lncRNA ENSG00000253385.1 in ESCC cells by fluorescence in situ hybridization (FISH). FISH and quantitative real-time polymerase chain reaction (qRT‒PCR) were used to detect gene expression in ESCC tissues and cells. …”
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Behavioural Risk for HIV, Hepatitis B, and Hepatitis C Infections among a Population of Drug Users and Injectors across Four Regions in Ghana by Chris Guure, Sharren Margaret Obiri-Yeboah Laryea, Samuel Dery, Carlota Baptista da Silva, Comfort Asamoah-Adu, Stephen Ayisi-Addo, Maria-Goretti Loglo, Adamu Mohammed, Kwasi Torpey

“…Efforts were made by the team to ensure adequate representation of women where feasible. A quantitative questionnaire was developed and used to obtain information on the respondents’ sociodemographics, sexual behavior, substance use, and biological characteristics. …”
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Stress and Coping of Parents with Children Seeking Treatment from a Tertiary Care Mental Health Institute by Stuti Sharma Lohar, Arunjyoti Baruah, Sandeep Kollipara

“…Materials and Methods: A quantitative approach and descriptive research design were adopted for this study. …”
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Equivalent experimental study on rock breaking of SC-CO2 and industrial emulsion explosive by Nan JIANG, Yingkang YAO, Bin ZHU, Xuedong LUO

“…Supercritical (SC)-CO2 rock breaking technology offers a safe alternative to industrial explosive blasting. Quantitatively calculating the rock breaking efficiency of SC-CO2 is crucial for comparing its engineering application and economic benefits against nonexplosive rock breaking methods. …”
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Targeting HVEM-GPT2 axis: a novel approach to T cell activation and metabolic reprogramming in non-small cell lung cancer therapy by Yuanshan Yao, Chunji Chen, Bin Li, Wen Gao

“…Co-cultured system and phenotype assays facilitated the examination of Jurkat T cells’ effect on A549 and H1299 lung cancer cells. Quantitative PCR and Western blotting determined gene and protein expression, respectively, cellular respiration was measured through oxygen consumption rate (OCR) assays. …”
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EFFECTS OF SMOKING ON CIRCADIAN ARTERIAL BLOOD PRESSURE PROFILE IN PATIENTS WITH NON-ST-SEGMENT ELEVATION ACUTE CORONARY SYNDROME AND CHRONIC KIDNEY DISEASE by Oksana Yadzhyn, Tetiana Solomenchuk

“…To study the renal functional status in the examined patients, the urine albumin-creatinine ratio (ACR) was determined using the semi-quantitative method. The glomerular filtration rate (GFR) was calculated using the CKD-EPI Creatinine Equation. …”
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Insights Into Causal Effects of Genetically Proxied Lipids and Lipid‐Modifying Drug Targets on Cardiometabolic Diseases by Liwan Fu, Qin Liu, Hong Cheng, Xiaoyuan Zhao, Jingfan Xiong, Jie Mi

“…Summary data for 10 cardiometabolic diseases were compiled from both discovery and replication data sets. Expression quantitative trait loci data from relevant tissues were employed to evaluate significant lipid‐modifying drug targets. …”
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Comparing Virtual Reality–Based and Traditional Physical Objective Structured Clinical Examination (OSCE) Stations for Clinical Competency Assessments: Randomized Controlled Trial... by Tobias Mühling, Verena Schreiner, Marc Appel, Tobias Leutritz, Sarah König

“…Student perceptions of the VRS were collected through a quantitative and qualitative postexamination online survey, which included a 5-point Likert scale ranging from 1 (minimum) to 5 (maximum), to evaluate the acceptance and usability of the VR system. …”
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A User-Centered Design Approach for a Screening App for People With Cognitive Impairment (digiDEM-SCREEN): Development and Usability Study by Michael Zeiler, Nikolas Dietzel, Fabian Haug, Julian Haug, Klaus Kammerer, Rüdiger Pryss, Peter Heuschmann, Elmar Graessel, Peter L Kolominsky-Rabas, Hans-Ulrich Prokosch

“…MethodsThis research used qualitative and quantitative methods and involved 3 key stakeholder groups: the digiDEM research team, the software development team, and the target user group (older adults ≥65 years with and without cognitive impairments). …”
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Simulation and analysis of marine hydrodynamics based on the El Niño scenario by M. Ikhwan, R. Wafdan, Y. Haditiar, M. Ramli, Z. Muchlisin, S. Rizal

“…To separate the signal produced and obtain the quantitative difference of each sea layer, the simulation results are analyzed using the fast Fourier transform. …”
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A Muscle Physiology-Based Framework for Quantifying Training Load in Resistance Exercises by Frank Imbach, Stéphane Perrey, Thomas Brioche, Robin Candau

“…Conclusions: A generic equation of muscle fatigue rise could add value to TL quantification in RE. Considering other training-related information and TL indexes stands essential, applicable to field situations and supports the multidimensional facet of physiological responses to RE.…”
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Human-genome single nucleotide polymorphisms affecting transcription factor binding and their role in pathogenesis by E. V. Antontseva, A. O. Degtyareva, E. E. Korbolina, I. S. Damarov, T. I. Merkulova

“…Thus, mapping expression quantitative trait loci (eQTLs) makes it possible to identify an SNP for which – among transcriptomes of homozygotes and heterozygotes for its various alleles – there are differences in the expression level of certain genes, which can be located at various distances from the SNP. …”
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Exploring the anticancer potential of extracts and compounds from the heartwood of Cotinus coggygria Scop. wild growing in Serbia by Ivana Pašić, Miroslav Novaković, Vele Tešević, Slobodan Milosavljević, Nina Petrović, Tatjana Stanojković, Ivana Z. Matić

“…The antimigration effects were examined by scratch assay, while expression of the MMP2, MMP9, and VEGFA were measured by quantitative real time PCR. The antioxidant effects were examined by flow cytometry. …”
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Seroprevalence characteristics of hepatitis E virus among blood donors infected with hepatitis B virus by YU Qin, XU Tingting, YANG Hao, ZHAO Lei

“…[Methods] From January to December 2022, 219 samples positive for hepatitis B surface antigen (HBsAg), 142 occult hepatitis B virus infection (OBI) samples (HBV group) and 873 samples tested negative (control group) were collected. 361 samples were further tested with viral load assay and serological testing for five serological markers (HBsAg, HBsAb, HBeAg, HBeAb and HBcAb), and the DNA load was measured using real time fluorescence quantitative PCR. Commercially available enzyme-linked immunosorbent assays (ELISA) were used for the detection of anti-HEV IgG, anti-HEV IgM and HEV antigen (Ag). …”
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