Identification of CENPM as a key gene driving adrenocortical carcinoma metastasis via physical interaction with immune checkpoint ligand FGL1 by Cunru Zou, Yu Zhang, Chengyue Liu, Yaxin Li, Congjie Lin, Hao Chen, Jiangping Hou, Guojun Gao, Zheng Liu, Qiupeng Yan, Wenxia Su

“…The impact of CENPM on metastasis behaviour was verified in ACC (H295R and SW‐13) cells and xenograft NPG mice. DIA quantitative proteomics analysis, western blot, immunofluorescence, and co‐immunoprecipitation assay were performed to identify the downstream target of CENPM. …”
Get full text

Application of fractal model in the Jurassic tight sandstone reservoirs of central Junggar Basin as constrained by mercury ejection curves by Hongkang Zhao, Xiangchun Chang, Tianchen Ge, Zhiping Zeng, Junjian Zhang, Daiqi Ming, Runye Han

“…However, the conventional analysis of the high-pressure mercury intrusion has always focused on the mercury injection curves themselves, neglecting the important geological information conveyed by the mercury ejection curves. This paper quantitatively describes the fractal characteristics of ejection curves by using four fractal models, i.e., Menger model, Thermodynamic model, Sierpinski model, and multi-fractal model. …”
Get full text

The study of miRNA-211 affects melanogenesis progress in Cashmere goats via suppressing <i>AP1S2</i> by B. Zhang, R. Liu, T. Ma, X. Li, Y. Zhao, H. Su, J. Li, Y. Li, H. Jiang, Q. Zhang

“…In B16-F10 cells, which overexpress miRNA-211 and goat skin, the opposite expression trends between <i>AP1S2</i> and miRNA-211 were analyzed by reverse transcription–quantitative PCR (RT-qPCR) and western blot. And their opposite expression trends were confirmed in B16-F10 cells by immunofluorescence. …”
Get full text

First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) by Paloma Kiwan, Eva Lopez, Morena Gasparine, Geraldine Piorkowski, Agathe Colmant, Achille Paguem, Stephanie Mvodo, Laurence Thirion, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi

“…(B.) microplus (472; 75.9%), Amblyomma variegatum (118; 19.0%), Hyalomma truncatum (13; 2.1%), Hyalomma rufipes (2; 0.3%), and other Rhipicephalus spp. (17; 2.7%). The quantitative reverse transcription polymerase chain reaction (qRT-PCR) screening of 251 tick pools yielded 61 JMTV-positive pools, of which 58 corresponded to R. …”
Get full text

Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…The inflammatory factor levels were performed by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). …”
Get full text

Prevalence of dengue and leptospirosis coinfection and associated mortality rates: a systematic review and meta-analysis by Suraj Tiwari, Mahalaqua Nazli Khatib, Rekha MM, Mandeep Kaur, Girish Chandra Sharma, Puneet Sudan, K. Satyam Naidu, Rajesh Singh, Teena Vishwakarma, Sonam Puri, Muhammed Shabil, Anuradha Yadav, Takveer Singh, Sahil Khurana, Rachana Mehta, Prakasini Satapathy, Renu Sah, Abhay M Gaidhane, Ganesh Bushi

“…Studies reporting quantitative data on the prevalence or mortality of dengue and leptospirosis co-infections were included. …”
Get full text

A Novel Convolutional Neural Network-Based Approach for Fault Classification in Photovoltaic Arrays by Farkhanda Aziz, Azhar Ul Haq, Shahzor Ahmad, Yousef Mahmoud, Marium Jalal, Usman Ali

“…This paper presents a novel approach that utilizes deep two-dimensional (2-D) Convolutional Neural Networks (CNN) to extract features from 2-D scalograms generated from PV system data in order to effectively detect and classify PV system faults. An in-depth quantitative evaluation of the proposed approach is presented and compared with previous classification methods for PV array faults &#x2013; both classical machine learning based and deep learning based. …”
Get full text

Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis by Bhagya Deepachandi, Sudath Weerasinghe, Himali Gunathilake, Thisira P. Andrahennadi, Mahendra N. Wickramanayake, Shantha Siri, Vishvanath Chandrasekharan, Preethi Soysa, Yamuna Siriwardana

“…Optimization included quantification of crude Ag, checkerboard titration method for determination of optimal concentrations for coating Ag, human sera and secondary antibody (Ab) with suitable coating buffer, blocking buffer, and incubating temperatures. …”
Get full text

Identification of highly expressed genes and efficient core promoters specific to buffalo skeletal muscles by J. Huang, J. Huang, D. Guo, R. Zhu, H. Wang, C. Yang, D. Shi, J. Shang

“…Among them, only two genes – namely, <i>VGLL2</i> and <i>CACNG1</i> – were identified to be specifically and efficiently expressed in the skeletal muscles of buffalo by quantitative reverse transcription polymerase chain reaction (RT-qPCR). …”
Get full text

Spatio-temporal Evolution of Land Surface Temperature and Its Response to LULC in the Urban Agglomeration of Central Yunnan by LIN Zhiqiang, PENG Shuangyun, SHI Shuangfu, HUANG Bangmei, MA Dongling, ZHU Ziyi, MA Xiaoliang, GONG Luping

“…[Methods] Based on Google Earth Engine (GEE) platform, LST was inverted using Landsat satellite image data of central Yunnan urban agglomeration region from 1990 to 2020, and was analyzed using Sen-MK trend analysis, mean-standard deviation method, urban thermal field variance index (UTFVI) and correlation analysis were used to quantitatively analyze the spatial and temporal variations of LST and its response to LULC types in central Yunnan urban agglomeration from 1990 to 2020. …”
Get full text

Development and predictors of bipolar disorder in children and adolescents with depressive disorders: a systematic review, meta-analysis, and meta-regression by Gonzalo Salazar de Pablo, Violeta Perez-Rodriguez, Javier de Otazu Olivares, Javier Camacho-Rubio, Aditya Sharma, Ana Catalán, Josefien Breedvelt, Claudia Aymerich, Mihai Pop, Carmen Moreno, Ian Kelleher, Jane Anderson, Paolo Fusar-Poli, Christoph U Correll, Allan H. Young

“…Abstract Background Estimating the risk of developing bipolar disorder (BD) in children and adolescents (C&A) with depressive disorders is important to optimize prevention and early intervention efforts. We aimed to quantitatively examine the risk of developing BD from depressive disorders and identify factors which moderate this development. …”
Get full text

Urinary exosomal miRNA-451a can be used as a potential noninvasive biomarker for diagnosis, reflecting tubulointerstitial damage and therapeutic response in IgA nephropathy by Qiong Zhang, Yan Zhao, Yankun Luo, Songjia Guo, Haizhu Hou, Xiaoli Han, Yun Zhou

“…The expression of miR-451a within exosomes was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). uE miR-451a was significantly upregulated in patients with IgAN compared to non-IgAN and HCs. …”
Get full text

Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell–Cell Fusion and Immune Activation by Yao Wang, Xunlei Pang, Ruirui Li, Jiuzhou Chen, Chen Wen, Huihuang Zhu, Tingyu Long, Jianjie Li, Lijun Zheng, Youcai Deng, Junnian Zheng, Bo Xu

“…Methods: We constructed a fusogenic protein, fusion-associated small transmembrane (FAST) p14 of reptilian reovirus, into cancer cells and mesenchymal stem cells (MSCs), which cocultured with various colon cancer cells and melenoma cells to validate its ability to induce cell fusion and syncytia formation. RNA sequencing, quantitative reverse transcription polymerase chain reaction, and Western blot were performed to elucidate the mechanism of syncytia death. …”
Get full text

The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine by Shukui Zhou, Ying Wang, Kaile Zhang, Nailong Cao, Ranxing Yang, Jianwen Huang, Weixin Zhao, Mahbubur Rahman, Hong Liao, Qiang Fu

“…Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. …”
Get full text

Evolution paths of urban forms influenced by landforms: Asynchrony but convergence by Chao Yang, Qiyan Huang, Qiong Xian, Meng Yuan, Xiaoming Cong, Lisha Xun, Jianxiong Cheng, Hongyi Pan

“…From 1990 to 2020, we quantitatively describe urban form characteristics using the two new indices alongside the landscape pattern index. …”
Get full text

Evaluation of nutrient loads conveyed to the deep subalpine lakes of Northern Italy through their main tributaries by Paolo Dezuanni, Paolo Dezuanni, Diego Copetti, Claudia Dresti, Nicolò Pella, Nicolò Pella, Fabio Buzzi, Andrea Fenocchi, Andrea Fenocchi

“…The LOAD ESTimator (LOADEST) software was adopted to support the load quantification process. Results show that a general decrease in external nutrient loads is still occurring, albeit at a slow pace, the fastest reduction occurring for the Oglio River flowing into Lake Iseo, which has suffered from severe nutrient pollution till recently. …”
Get full text

Meals, Education, and Gardens for In-School Adolescents (MEGA): study protocol for a cluster randomised trial of an integrated adolescent nutrition intervention in Dodoma, Tanzania by Wafaie W Fawzi, Dongqing Wang, Abbas Ismail, Leonard Kamanga Katalambula, Andrea R Modest, Tara Young, Mary Mwanyika-Sando, Amani Tinkasimile, Dominic Mosha, Augustine Malero, Said Vuai

“…Baseline and end-line quantitative data collection will include 750 adolescents and 750 parents. …”
Get full text

Biochar effects on durum wheat (Triticum durum) under ambient and elevated atmospheric CO2 by Lorenzo Brilli, Franz-Werner Badeck, Lorenzo Genesio, Silvia Baronti, Fulvia Rizza, Clara Fares, Luigi Cattivelli, Franco Miglietta, Anita Maienza, Alessandro Zaldei, Federica Camin, Rosella Motzo, Piero Toscano

“…To test this hypothesis, we present the results of an open field experiment investigating the combined effects of biochar application and e[CO2] on quantitative and qualitative performance of two commercial durum wheat (Triticum durum) cultivars (Aureo and Claudio). …”
Get full text

Identification and preliminary validation of biomarkers associated with mitochondrial and programmed cell death in pre-eclampsia by Rong Lin, Rong Lin, XiaoYing Weng, XiaoYing Weng, Liang Lin, Liang Lin, XuYang Hu, XuYang Hu, ZhiYan Liu, ZhiYan Liu, Jing Zheng, Jing Zheng, FenFang Shen, FenFang Shen, Rui Li, Rui Li

“…Clinical validation was conducted via real-time quantitative polymerase chain reaction (RT-qPCR), immunofluorescence, and Western blot.ResultsFour genes [solute carrier family 25 member 5 (SLC25A5), acyl-CoA synthetase family member 2 (ACSF2), mitochondrial fission factor (MFF), and phorbol-12-myristate-13-acetate–induced protein 1 (PMAIP1)] were identified as biomarkers distinguishing PE from normal controls. …”
Get full text

Associations of early life per- and polyfluoroalkyl substances (PFAS) exposure with body mass index and risk of overweight or obesity at age 2–18 years: Mixture analysis in the pro... by Zeyu Li, Guoying Wang, Joseph M. Braun, Xiumei Hong, Giehae Choi, Shawn P. O’Leary, Chang Ho Yu, Colleen Pearson, William G. Adams, Zhihua (Tina) Fan, Jessie P. Buckley, Xiaobin Wang

“…The exposure-outcome associations were evaluated with linear and modified Poisson mixed-effects regression for individual PFAS and Bayesian kernel machine regression and quantile-based g-computation models for PFAS mixture. …”
Get full text