Adolescentes autores de agressão sexual: Percepções sobre infância, adolescência e violência. by Viviam da Silva Silveira, Lília Iêda Chaves Cavalcante, Daniela Castro dos Reis

“…Constitui-se em uma pesquisa com delineamento de natureza empírico-descritiva e uma análise de caráter quanti-qualitativa dos dados, envolvendo adolescentes em cumprimento de medida socioeducativa por ato infracional análogo ao estupro de vulnerável em unidades de atendimento socioeducativo no norte do país. …”
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Concepções de fracasso escolar: um estudo com professores das cinco regiões brasileiras by Monica de Araújo Damasceno, Tatiane dos Santos Costa, Fauston Negreiros

“…Tratou-se de uma pesquisa quanti-qualitativa, do tipo ex post facto que utilizou dados transversais. …”
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UMA ANÁLISE CRIMINOLÓGICO-CRÍTICA SOBRE OS DISCURSOS DISCENTES ACERCA DA CRIMINALIZAÇÃO DA HOMOFOBIA by Túlio Vinícius Andrade Souza, Maria Cristina Lopes de Almeida Amazonas

“…Os dados coletados foram tabulados e analisados através de uma abordagem quanti-qualitativa, demonstrando, sobretudo, que a maioria dos pesquisados não conhecia o Projeto de Lei e, ainda, resultando em categorias argumentativas importantes para a análise dos posicionamentos dos discentes. …”
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Feedback of aggregate patient-reported outcomes (PROs) data to clinicians and hospital end users: findings from an Australian codesign workshop process by Monique F Kilkenny, Dominique A Cadilhac, Shaun L Hancock, Violet Marion, Paulette Kelly, Sibilah Breen, Benjamin Clissold, Lauren Sanders, Olivia Francis Ryan, Penina Gunzburg, Shae Cooke, Lauren Guy

“…For each outcome measure, quantitative and qualitative data were summarised.Results 31 Working Group members (19 stroke, 2 psychology, 1 pharmacy, 9 researchers) participated in the workshops, where 25/55 templates were shortlisted for wider assessment. …”
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SNORA37/CMTR1/ELAVL1 feedback loop drives gastric cancer progression via facilitating CD44 alternative splicing by Banghe Bao, Minxiu Tian, Xiaojing Wang, Chunhui Yang, Jiaying Qu, Shunchen Zhou, Yang Cheng, Qiangsong Tong, Liduan Zheng

“…Alternative splicing and gene expression was observed by real-time quantitative RT-PCR and western blot assays. In vitro and in vivo studies were performed to investigate biological effects of snoRNAs and their protein partners in gastric cancer. …”
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CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…Immunofluorescence staining was established and done for 10 organoid lines for cystic fibrosis transmembrane conductance regulator (CFTR) and KRT81. Quantitative real-time PCR (qPCR) was performed for CFTR and HNF1A. …”
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Oxidative Stress Induced Damage and Early Senescence in Preterm Placenta by Yudianto Budi Saroyo, Noroyono Wibowo, Rima Irwinda, Ani Retno Prijanti, Evy Yunihastuti, Saptawati Bardosono, Sofie Rifayani Krisnadi, Putri Indah Permata, Stephanie Wijaya, Victor Prana Andika Santawi

“…Real-time polymerase chain reaction was performed to measure T/S ratio and ELISA quantification to measure the amount of HMGB1 and 8-OHdG. …”
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Molecular Alterations of KIT Oncogene in Gliomas by Ana L. Gomes, Jorge S. Reis-Filho, José M. Lopes, Olga Martinho, Maryou B. K. Lambros, Albino Martins, Fernando Schmitt, Fernando Pardal, Rui M. Reis

“…KIT activating gene mutations (exons 9, 11, 13 and 17) and gene amplification analysis, as defined by chromogenic in situ hybridization (CISH) and quantitative real-time PCR (qRT-PCR) were performed in CD117 positive cases. …”
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Gas-phase fractionation DDA promotes in-depth DIA phosphoproteome analysis by Zhiwei Tu, Yabin Li, Shuhui Ji, Shanshan Wang, Rui Zhou, Gertjan Kramer, Yu Cui, Fei Xie

“…Data-independent acquisition (DIA) is a promising method for quantitative proteomics. Library-based DIA database searching against project-specific data-dependent acquisition (DDA) spectral libraries is the gold standard. …”
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Optimization of a micro-scale air–liquid-interface model of human proximal airway epithelium for moderate throughput drug screening for SARS-CoV-2 by Chandani Sen, Tammy M. Rickabaugh, Arjit Vijey Jeyachandran, Constance Yuen, Maisam Ghannam, Abdo Durra, Adam Aziz, Kristen Castillo, Gustavo Garcia, Arunima Purkayastha, Brandon Han, Felix W. Boulton, Eugene Chekler, Robert Garces, Karen C. Wolff, Laura Riva, Melanie G. Kirkpatrick, Amal Gebara-Lamb, Case W. McNamara, Ulrich A. K. Betz, Vaithilingaraja Arumugaswami, Robert Damoiseaux, Brigitte N. Gomperts

“…We applied this model to screen 2100 compounds from targeted drug libraries using a high throughput-high content image-based quantification method. Results The model recapitulated the heterogeneity of infection among patients with SARS-CoV-2 parental strain and variants. …”
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Spatiotemporal optimization of irrigation practices for winter wheat in China: Rationale, implications, and solutions by Luchen Zhang, Yuan Cao, Weihao Qian, Junning Tian, Shengshi Huang, Xiaolei Qiu, Bing Liu, Liang Tang, Liujun Xiao, Weixing Cao, Yan Zhu, Leilei Liu

“…The findings offer a method for quantitatively predicting and warning of the impacts of drought stress, and meanwhile support the formulation of irrigation strategies that maximize crop yield and water use efficiency and reducing water consumption in China.…”
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Detection of Circulating Tumor DNA in Patients with Thyroid Nodules by Krupal B. Patel, Nicholas Cormier, James Fowler, Allison Partridge, Julie Theurer, Morgan Black, Nicole Pinto, John Yoo, Kevin Fung, Danielle MacNeil, William Stecho, Christopher Howlett, Muriel Brackstone, John W. Barrett, Anthony Nichols

“…Plasma samples were obtained preoperatively and one month postoperatively. Quantitative PCR was used to determine the levels of the BRAF (V600E) mutation preoperatively and postoperatively. …”
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Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Comprehensive analysis of mRNA and miRNA differential expression profiles in the hypothalamus-pituitary-gonadal axis in laying and broodiness period of Wanxi white geese by Xinwei Tong, Xiaojin Li, Yuhua Wang, Fei Xie, Ruidong Li, Man Ren, Qianqian Hu, Shenghe Li

“…Six DEGs and four DEMs were randomly selected for real-time fluorescence quantitative reverse transcription PCR (RT-qPCR). The results showed that the transcriptome sequencing data were accurate and reliable. …”
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Protocol for a feasibility study and process evaluation of a psychosocially modelled diabetes education programme for young people with type 1 diabetes: the Youth Empowerment Skill... by Nick Sevdalis, Khalida Ismail, Tayana Soukup, Trevor Murrells, Dulmini Kariyawasam, Stephen Thomas, Judith Parsons, Angus Forbes, Rita Forde, Maria Baldellou Lopez, Marie Jones, Martha Ford-Adams, Nardos Yemane, Siobhan Pender, Alex Silverstien

“…A simultaneous process evaluation will use a mixed-methods approach collecting qualitative and quantitative data. Study findings will be used to optimise the intervention components, outcome measures and recruitment methods to inform a subsequent definitive trial.Ethics and dissemination The protocol has ethical approval from the UK Health Research Authority (approval IRAS project ID: 279877). …”
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Integrating Indigenous healing practices within collaborative care models in primary healthcare in Canada: a rapid scoping review by Ginny Brunton, Pierre Côté, Silvano Mior, Hainan Yu, Anne Taylor-Vaisey, Melissa Corso, Astrid DeSouza, Carolina Cancelliere, Kathy MacLeod-Beaver

“…This rapid scoping review aimed to identify and synthesise strategies used to integrate Indigenous healing practices within collaborative care models available in community-based primary healthcare, delivered by regulated health professionals in Canada.Eligibility criteria We included quantitative, qualitative and mixed-methods studies conducted in community-based primary healthcare practices that used strategies to integrate Indigenous healing practices within collaborative care models.Sources of evidence We searched MEDLINE, Embase, Indigenous Studies Portal, Informit Indigenous Collection and Native Health Database for studies published from 2015 to 2021.Charting methods Our data extraction used three frameworks to categorise the findings. …”
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Association between leukocyte telomere length and specific antibody levels after vaccination against tick-borne encephalitis by N. S. Yudin, V. A. Belyavskaya, V. N. Maksimov, D. E. Ivanoshchuk, P. S. Orlov, M. I. Voevoda

“…One month after vaccination, the level of specific IgG antibodies against tick-borne encephalitis virus was analyzed using the VektoVKE-IgG-strip test system and leukocyte telomere length was measured using real-time quantitative PCR. According to the intensity of vaccine-elicited immune responses, patients were divided into three groups: unresponsive (IgG level 0–100 IU/ml), slightly responsive (IgG level 101–200 IU/ml) and highly responsive (IgG level above 200 IU/ml). …”
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Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficits by Yiying Hu, Alexander Hruscha, Chenchen Pan, Martina Schifferer, Michael K. Schmidt, Brigitte Nuscher, Martin Giera, Sarantos Kostidis, Özge Burhan, Frauke van Bebber, Dieter Edbauer, Thomas Arzberger, Christian Haass, Bettina Schmid

“…Behavior was investigated by video tracking and quantitative analysis of swimming parameters. RNA sequencing was used to identify mis-regulated pathways with validation by molecular analysis. …”
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A prognostic signature of Glutathione metabolism-associated long non-coding RNAs for lung adenocarcinoma with immune microenvironment insights by Junxi Hu, Junxi Hu, Shuyu Tian, Shuyu Tian, Qingwen Liu, Qingwen Liu, Jiaqi Hou, Jiaqi Hou, Jun wu, Jun wu, Xiaolin Wang, Xiaolin Wang, Yusheng Shu, Yusheng Shu

“…Functional analysis revealed immune infiltration and drug sensitivity differences. Quantitative PCR and experimental studies confirmed the role of lnc-AL162632.3 in LUAD.ResultsOur model included a total of nine lncRNAs, namely AL162632.3, AL360270.1, LINC00707, DEPDC1-AS1, GSEC, LINC01711, AL078590.2, AC026355.2, and AL096701.4. …”
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Azithromycin Promotes the Osteogenic Differentiation of Human Periodontal Ligament Stem Cells after Stimulation with TNF-α by Tingting Meng, Ying Zhou, Jingkun Li, Meilin Hu, Xiaomeng Li, Pingting Wang, Zhi Jia, Liyu Li, Dayong Liu

“…After stimulating PDLSCs with TNF-α and AZM, we analyzed alkaline phosphatase staining, alkaline phosphatase activity, and alizarin red staining to detect osteogenic differentiation. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed to detect the mRNA expression of osteogenic-related genes, including RUNX2, OCN, and BSP. …”
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