Extraction of tree branch skeletons from terrestrial LiDAR point clouds by Jimiao Gao, Liyu Tang, Honglin Su, Jiwei Chen, Yuehui Yuan

“…The wood points and tree skeletons are further used to estimate tree attributes, demonstrating the potential of our method for the quantitative representation of trees and their ecological characteristics (e.g., carbon sequestration).…”
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miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression by Feng Tian, Ping Tang, Zhilian Sun, Ruifen Zhang, Danhua Zhu, Junying He, Jixing Liao, Qinghua Wan, Jie Shen

“…Expression of miR-210 was determined by quantitative RT-PCR. Glucose uptake was measured by a fluorometric method, and the mitochondrial respiratory chain activity was evaluated by ELISA. …”
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Investigating the potential immunomodulatory effects of commercial oral probiotic supplements on equine gastrointestinal tract barrier function by Agnieszka Żak-Bochenek, Paulina Żebrowska-Różańska, Joanna Bajzert, Łukasz Łaczmański, Bogumiła Szponar, Natalia Siwińska, Klaudia Gładysz, Katarzyna Sikorska, Anna Chełmońska-Soyta

“…The samples were subjected to microbiome analysis via next-generation sequencing of hypervariable regions V3-V4 and V7-V9 of the 16S rRNA gene for analysis of short-chain fatty acids via HPLC analysis and fecal secretory immunoglobulin A (SIgA) quantification via ELISA.ResultsMicrobiome analysis revealed no significant differences in either alpha or beta diversity parameters between the groups. …”
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Automatic GPR detection of grouting defects behind the tunnel shield segments based on wavelet coherence analysis combined with modified Res-RCNN by Dengyi Wang, Ming Peng, Liu Liu, Xiongyao Xie, Zhenming Shi, Yaoying Liang, Jian Shen, Qiyu Wu

“…The network demonstrates superior training convergence and prediction accuracy due to information sharing between different task heads, compared to a two-classification network with the same Res-Net backbone. Through quantitative experiments in both model and synthetic tests, we recommend a trace interval of 15 to avoid the high coherence amplitude caused by two reflections out of same individual rebar.…”
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A Morphological and Immunohistochemical Study of the Tumoral and Inflammatory Cells in Pancreatic Ductal Adenocarcinoma by Vlad Herlea, Elena Stoica Mustafa, Andreea Cristina Iorgescu, Nicolae Catalin Pechianu, Dragos Cretoiu, Maria Sajin, Simona Olimpia Dima, Catalin Vasilescu, Cezar Stroescu, Constantin Ungureanu, Traian Dumitrascu, Vladislav Brasoveanu, Minerva Ghinescu, Irinel Popescu

“…It is recommended to study peritumoral tissue from the quantitative and qualitative points of view to increase the number of prognostic factors. …”
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Impact of meropenem exposure on fluoroquinolone and carbapenem resistance in Pseudomonas aeruginosa infection in inpatients in a Japanese university hospital: Insights into oprD mu... by Tadanori Yamochi, Kazuhisa Ugajin, Rintaro On, Sho Inoue, Hiromi Ikeda, Toshiko Yamochi, Masafumi Takimoto, Issei Tokimatsu

“…The minimal inhibitory concentrations of clinically relevant antibiotics were determined. Quantitative PCR was performed to analyze oprD, mexB, mexC, mexE, and mexY expression. …”
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Insulin-Attenuated Inflammatory Response of Dendritic Cells in Diabetes by Regulating RAGE-PKCβ1-IRS1-NF-κB Signal Pathway: A Study on the Anti-Inflammatory Mechanism of Insulin in... by Liding Zhao, Ya Li, Qingbo Lv, Min Wang, Yi Luan, Jiale Song, Guosheng Fu, Junbo Ge, Yunzeng Zou, Wenbin Zhang

“…The fluctuations in the RNA levels of cytokines and chemokines were analyzed by quantitative RT-PCR. The concentrations of IFN-γ and TNF-α were calculated using ELISA kits, and the proteins were detected using western blot. …”
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Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction by Hongjian Zhou, Zhipeng Xu, Chun Jiang, Qiuyue Wu, Chuanyue Zhang, Zhenyu Liu, Xiaoxue Zhang, Weiwei Li, Yujia Pang, Jing Zhang, Wenju Pan, Min Chen, Xinyi Xia

“…Histological examination, sperm concentration and motility analysis, Western blotting (WB), and reverse transcription quantitative PCR (RT-qPCR) were used to evaluate testicular injury. …”
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Dissecting Causal Relationships Between Antihypertensive Drug, Gut Microbiota, and Type 2 Diabetes Mellitus and Its Complications: A Mendelian Randomization Study by He Zheng, Shiping Wu, Wenbin Wang, Weida Qiu, Yingqing Feng

“…Genetic instruments for the expression of antihypertensive drug target genes were identified with expression quantitative trait loci (eQTL) in blood, which should be associated with systolic blood pressure (SBP). …”
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Game mechanics in eHealth interventions promoting self-management in young people with chronic diseases: a protocol for a systematic review and meta-analyses from the eHealth Junio... by Sanne L Nijhof, Elise van de Putte, Fernando Estévez-López, Wichor M Bramer, Lisa Levelt, Job van ’t Veer, Micah M Hrehovcsik, Valentijn Visch, Manon H. J. Hillegers, Remco C Veltkamp

“…When possible, the effects of the gamified interventions will be compared with non-gamified interventions or care-as-usual. Primary quantitative, qualitative or mixed-method studies written in English will be included. …”
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Efforts to strengthen anatomic pathology diagnostic services for cancer in sub-Saharan Africa: a scoping review protocol by Katherine Van Loon, Elia John Mmbaga, Marcia Edelweiss, Dianna L. Ng, Asteria Herman Kimambo, Edda Vuhahula, Beatrice Paul Mushi, Omolade Adegoke, Johanna Goldberg, Pendo M. Ibrahim, Zainab Illonga, Julia Jackman, Luisa Jamisse

“…All study designs will be considered, including quantitative, qualitative and/or mixed-methods studies.Ethics and dissemination Ethical approval is not required for this study. …”
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Analysis of the spatial pattern and causes of ecological environmental quality in Myanmar based on the RSEI model and the Geodetector-GCCM method by Shuangfu Shi, Shuangfu Shi, Shuangyun Peng, Shuangyun Peng, Zhiqiang Lin, Zhiqiang Lin, Bangmei Huang, Dongling Ma, Dongling Ma, Ziyi Zhu, Ziyi Zhu, Yilin Zhu, Yilin Zhu, Rui Zhang, Ting Li

“…This study took Myanmar as the research area, employing a Remote Sensing Ecological Index (RSEI) model and spatial autocorrelation analysis to quantitatively evaluate the spatial distribution characteristics of Myanmar’s EEQ in 2020 and reveal its spatial dependence. …”
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Monitoring changes of forest height in California by Samuel Favrichon, Jake Lee, Yan Yang, Yan Yang, Ricardo Dalagnol, Ricardo Dalagnol, Fabien Wagner, Le Bienfaiteur Sagang, Le Bienfaiteur Sagang, Sassan Saatchi, Sassan Saatchi, Sassan Saatchi

“…Height showed distinct spatial variations across forest types offering quantitative and spatial information to evaluate forest conditions. …”
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Identification of a transcription factor AoMsn2 of the Hog1 signaling pathway contributes to fungal growth, development and pathogenicity in Arthrobotrys oligospora by Qianqian Liu, Kexin Jiang, Shipeng Duan, Na Zhao, Yanmei Shen, Lirong Zhu, Ke-Qin Zhang, Jinkui Yang

“…Methods: The function of AoMsn2 was characterized using targeted gene deletion, phenotypic experiments, real-time quantitative PCR, RNA sequencing, untargeted metabolomics, and yeast two-hybrid analysis. …”
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Periodontitis promotes tumor growth and immune evasion via PD-1/PD-L1 by Suli Wang, Fujiao Nie, Qiuyue Yin, Haoyang Tian, Pizhang Gong, Jinhong Ju, Jiayi Liu, Pishan Yang, Chengzhe Yang

“…THP-1 cells were stimulated using conditioned media of LPS-stimulated Cal-27 cells and PD-L1 expression was measured by quantitative real-time PCR, western blotting and immunofluorescence. …”
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The Prevalence of Metabolic Syndrome and Hypertriglyceridemic Waist Based on Sociodemographic Variables and Healthy Habits in Healthcare Workers: A Retrospective Study by Pedro Javier Tárraga Marcos, Ángel Arturo López-González, Emilio Martínez-Almoyna Rifá, Hernán Paublini Oliveira, Cristina Martorell Sánchez, Pedro Juan Tárraga López, José Ignacio Ramírez-Manent

“…Descriptive analysis included categorical and quantitative variables, which were assessed through frequencies, Student’s <i>t</i>-test, chi-square, and binary logistic regression models. …”
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Identification of therapeutic target genes for age-related hearing loss through systematic genome-wide mendelian randomization of druggable genes by Kun Zhang, Bo Hou, Tao Yan, Ruru Qiao, Peng Qu, Xinbo Xu, Hanbing Zhang

“…Methods: We obtained data on the expression quantitative trait locis (eQTLs) of druggable genes, which were then subjected to two-sample MR analyses and co-localisation analyses with data from the ARHL genome-wide association study to identify genes highly associated with ARHL. …”
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Determining the mechanism of Shuxuening injection against liver cirrhosis through network pharmacology and animal experiments by Qiyao Liu, Tingyu Zhang, Yongan Ye, Xin Sun, Huan Xia, Xu Cao, Xiaoke Li, Wenying Qi, Yue Chen, Xiaobin Zao

“…Serum and liver tissues of the mice were collected for the detection of indicators, pathological staining, and expression analysis of hub targets using quantitative real-time polymerase chain reaction (qRT-PCR). …”
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Essential newborn care practices in selected public health facilities using observation of 2603 normal deliveries in Uttar Pradesh, India by James Blanchard, Marissa L Becker, Ties Boerma, Ravi Prakash, Shajy Isac, Bidyadhar Dehury, Banadakoppa Manjappa Ramesh, Vasanthakumar Namasivayam, John Anthony, Nihal Hasan, Prakash P Javalkar

“…This paper aims to understand the facility-level variations and factors associated with the essential newborn care practices by providers in higher-level public health facilities in 25 high priority districts (HPDs) of Uttar Pradesh (UP).Methods We used observational cross-sectional quantitative data from 48 selected public health facilities (23 district hospitals (DH) and 25 community health centres (CHC)—first referral units (FRU)) implemented in 25 HPDs of UP from February 2020 to May 2021. …”
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Ecdysone receptor strongly influences larval–pupal–adult transition and melanization in Tuta absoluta by Xiaodi Wang, Jiajia Wu, Jianyang Guo, Nianwan Yang, Fanghao Wan, Zhichuang Lü, Wanxue Liu

“…Methods In this study, RNA extraction, cDNA synthesis, gene cloning, bioinformatics analysis, and quantitative real-time polymerase chain reaction were used to obtain the full length, conserved domain, and relative expression levels of the EcR. …”
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