Evaluation of exo-long noncoding RNA MALAT1 in OSCC in comparison to dysplastic and normal: A cross-sectional study by Ramya Sekar, Selvaraj Jayaraman, VishnuPriya Veeraraghavan, Kalaiselvi Krishnamoorthy, Shubhra Chauhan Aramanai, Sathan Raj Natarajan

“…RNA concentration was estimated, real-time polymerase chain reaction (qPCR) was used for quantitative analysis. Gene expression levels of MALAT1, PI3K, AKT1, and PTEN were analysed and compared across groups using one way ANOVA and Post-hoc Tukey analysis was performed for pairwise comparisons to assess correlations between MALAT1 expression and PI3K/AKT pathway components. …”
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Unraveling the individual and interactive effects of climate and competition on branch growth dynamics in Pinus koraiensis in Northeast China by Xuehan Zhao, Zheng Miao, Zheng Miao, Fengri Li, Fengri Li, Yuanshuo Hao, Yuanshuo Hao, Yumeng Jiang, Lihu Dong, Lihu Dong

“…IntroductionThe quantitative modeling of dynamic branch growth in Korean pine (Pinus koraiensis) and the analysis of the factors influencing branch growth are essential prerequisites for making scientifically sound management decisions in Korean pine plantations. …”
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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“…Western blot analysis confirmed ATF4 suppression and increased expression of membrane proteins, including E-cadherin and CD63. Quantitative analysis via PAGE revealed a 77.2 ± 30.6% increase in purified membrane protein yields, consistent with the observed enhancements. …”
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Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation by Shuling Zhou, Yu Lei, Ping Wang, Jianying Chen, Liting Zeng, Ting Qu, Martin Maldonado, Jihua Huang, Tingting Han, Zina Wen, Erpo Tian, Xiangqian Meng, Ying Zhong, Jiang Gu

“…Angiogenesis capacity of interleukin-1 beta (IL-1β)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. …”
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Urban and rural residence: their influence on food group consumption in Iran by Susan Darroudi, Sara Saffar Soflaei, Farzam Kamrani, Zahra Khorasanchi, Zahra Abdollahi, Nasrin Talkhi, Maryam Allahyari, Seyyed Reza Sobhani, Maryam Mohammadi-Bajgiran, Amirmohammad Naderkhmseh, Maliheh Aghasizadeh, Habibollah Esmaily, Gordon Ferns, Majid Ghayour-Mobarhan

“…Their food intake was assessed using a validated dish-based semi-quantitative food frequency questionnaire (DB-FFQ). Results Rural males consumed significantly more grains (35.51 g/day, p = 0.03) than urban males, while rural females consumed significantly less dairy (-30.07 g/day, p = 0.03) than urban females. …”
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Response of plant life forms and soil physical properties to near-natural restoration measures in alpine grassland, Tibetan plateau by Guoxing He, Guoxing He, Xiaoni Liu, Xiaoni Liu, Yali Li, Yali Li, Tong Ji, Tong Ji

“…We utilized redundancy analysis (RDA), variation partitioning(VP), hierarchical partitioning (HP), and partial least squares pathway modeling (PLS-PM) to dissect the quantitative relationships between the distribution of plant life forms and soil physical properties under these restoration measures.Results and discussionThe results indicated the following: 1) Under each restoration measure, the distribution of life form plants were predominantly characterized by the highest number of hemicryptophytes, followed by geophytes, with the least number of therophytes. …”
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Developing a core outcome set for assessing interventions and care for parents after neonatal death in high-income countries (iCHOOSE Neonatal study): protocol for a mixed-methods... by James Webbe, Mehali Patel, Danya Bakhbakhi, Christy Burden, Mary Lynch, Laura Timlin, Lisa Hinton, Abigail Fraser, Anna Davies, Maggie Redshaw, David Odd, Charlotte Williams, Dimitris Siasakos, Abi Merriel, Sadie Mullin, Lucy Moy, Leanne Watts

“…A parent involvement group and stakeholder steering committee have been established and have informed each planned phase: (1) systematic review of quantitative studies evaluating care and interventions provided after neonatal death, to describe interventions, outcomes and outcome measurement tools used to assess intervention effectiveness; (2) qualitative interviews with parents who have experienced neonatal death to identify outcomes important and relevant to them; (3) think-aloud interviews with stakeholders (bereaved parents, healthcare professionals and other stakeholders) to develop and refine an online survey; (4) real-time online international Delphi survey with bereaved parents, healthcare professionals and other stakeholders to shortlist outcomes for consideration in the COS; (5) adapted nominal group online consensus meetings with parents, healthcare professionals and other stakeholders to agree final COS and (6) identification of a preliminary set of measurement tools.Ethics and dissemination Ethical approval has been granted for all activities to be undertaken by the University of Bristol Health Sciences Faculty Research Ethics Committee (reference: 15121). …”
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Sustainability of healthcare system improvements, programmes and interventions in acute care settings: protocol for a mixed methods systematic review by Franz E Babl, Sandy Middleton, Julie Cowie, Catherine Wilson, Peter Wilson, Pauline Campbell, Emma Tavender, Lisa Kuhn, Nicola Straiton, Victoria Ramsden, Elizabeth McInnes

“…Relevant reference lists of included studies will be manually searched. Empirical quantitative and qualitative studies that report the sustainability of an intervention or programme in acute care settings using a theoretical framework(s), model(s) or theory(ies) to explore facilitators and barriers, will be included. …”
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Determination of Puberulic Acid in Monascus-Fermented Red Yeast Rice by LC-MS/MS Combined with Precolumn Derivatization by Hui-Qin Pan, Rui Feng, Yan-Nan Tan, Xiao-Ya Qin, Yi-Min Cao, Xiu-Hong Mao, Qing Hu, Heng Zhou

“…The limit of detection and the limit of quantification could reach 2 μg/kg and 50 μg/kg, respectively. …”
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Impact of #PCOSweightloss: A global X hashtag analysis study of weight loss narratives in the PCOS community by Munaza Afaq, Kumar Vashisht, Areen Al-Dhoon, Divya Abraham, Atanas G. Atanasov, Maima Matin, Zara Arshad, Rahul Kashyap, Faisal A. Nawaz

“…Methods Fedica research analytics tool ( https://fedica.com ) was utilized to automatically evaluate quantitatively the posts on the platform X containing #PCOSweightloss from July 1st, 2017 to July 1st, 2023. …”
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Threshold responses of freshwater fish community size spectra to invasive species by Bradley Novak, Brent A. Murry, Jeff S. Wesner, Vojsava Gjoni, Caroline C. Arantes, Erin Shepta, Justin P. F. Pomeranz, James R. Junker, Kathrine Zipfel, Andrew Stump, Levi E. Solomon, Kristopher A. Maxson, Jason A. DeBoer

“…The introduction of Silver Carp (Hypophthalmichthys molitrix) throughout the mid‐western United States epitomizes both the impacts of AIS and the need for quantitative control targets. Silver Carp are large‐bodied planktivores that compete with native planktivores, which can cause cascading effects throughout the food web. …”
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Rhoifolin Suppresses Cell Proliferation and Induces Apoptosis in Hepatocellular Carcinoma Cells In Vitro and In Vivo by Ruolan Chen, Zufa Sabeel, Lu Ying, Youfeng Liang, Rui Guo, Mingxuan Hao, Xiaoyang Chen, Wenjing Zhang, Jian Dong, Yan Liu, Changyuan Yu, Zhao Yang

“…<b>Methods:</b> The suppressive effect of ROF on HCC cells were assessed via CCK8 assay, apoptosis assay, cell cycle analysis and xenograft tumor mouse model. Furthermore, quantitative real-time PCR and western blot were applied to analyze the underlying mechanisms of ROF on HCC cells. …”
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Analysis of Basketball Trainers Views about Coronavirus-19 Phobia by Dr. Yahya Gökhan Yalçın, Havva Demırel, Mehmet Altın

“…Quantitative research models were used in the study, which aimed to to assess the phobia related to the coronavirus-19 of basketball coaches working in Turkey Basketball Federation (TBF) and registered to Turkey's Basketball Coaches Association (TÜBAD). 21 women, and 216 men, 237 active basketball coaches in total, who are TÜBAD members at various levels by the Turkish Leagues, participated to the study. …”
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Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs by Takahiro Teshima, Akito Matsuoka, Maika Shiba, Kazuho Dairaku, Hirotaka Matsumoto, Ryohei Suzuki, Hidekazu Koyama

“…Secretome analysis was performed using iTRAQ and quantitative cytokine arrays. Over 1900 proteins were detected in conditioned medium (CM) of LDSCs and ADSCs, and 94.0% of detected proteins showed similar expression levels between CM of both cell types. …”
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Metabolic Syndrome and Its Components Predict the Risk of Type 2 Diabetes Mellitus in the Mainland Chinese: A 3-Year Cohort Study by Kun Wang, Qun-Fang Yang, Xing-Lin Chen, Yu-Wei Liu, Sheng-Shuai Shan, Hua-Bo Zheng, Xiao-Fang Zhao, Chang-Zhong Chen, Cheng-Yun Liu

“…MetS predicts the risk of T2DM. The quantitative MetS-based prediction model for T2DM risk may improve preventive strategies for T2DM and present considerable public health benefits for the people in mainland China.…”
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Associations between TGF-β1 Levels and Markers of Hemolysis, Inflammation, and Tissue Remodeling in Pediatric Sickle Cell Patients by Rayra P. Santiago, Magda O. S. Carvalho, Camylla V. B. Figueiredo, Luciana M. Fiuza, Rodrigo M. Oliveira, Sètondji C. M. A. Yahouédéhou, Valma M. L. Nascimento, Isa M. Lyra, Théo Araujo-Santos, Nívea F. Luz, Milena M. Aleluia, Caroline C. Guarda, Valéria M. Borges, Marilda S. Goncalves

“…TGF-β1, TIMP-1, and MMP-9 plasma quantifications were performed by ELISA. TGF-β1 plasma levels were higher in HbSS individuals than in HbSC and HC. …”
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The Length-Weight Relationship and Condition Factors of Bullet Tuna Landed at the Tanjung Luar Fishing Port, Indonesia by Evron Asrial, Yasir Arapat, Usma Kurniawan Hadi, Lalu A.T.T.W.S Kalih, Mita Ayu Liliyanti, Erwin Rosadi, Ruly Isfatul Khasanah, Ishani Nelunika Rathnayake

“…The primary and secondary data were processed quantitatively using several equations. The growth pattern of bullet tuna was minor allometric (b = 2.875), worth selling (K > 1.00), and the majority was adult fish or mature group. …”
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Cardiac Abnormalities in Acromegaly Patients: A Cardiac Magnetic Resonance Study by Xiaopeng Guo, Jian Cao, Peijun Liu, Yihan Cao, Xiao Li, Lu Gao, Zihao Wang, Ligang Fang, Zhengyu Jin, Yining Wang, Bing Xing

“…We aimed to evaluate the frequencies of left ventricular hypertrophy (LVH), interventricular septum hypertrophy (IVSH), LV systolic dysfunction (LVSD), right ventricular systolic dysfunction (RVSD), and myocardial fibrosis (MCF) and detailed quantitative parameters in acromegaly patients using CMR and analyze their correlations with clinical features. …”
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JAK/STAT3 signaling promotes pain and depression-like behaviors in rats with bone cancer pain by regulating Th17 cell differentiation by Shuyan Wu, Jundan Jiang, Danfeng Wang, Daoyi Lin, Mingxue Lin, Pinzhong Chen, Jianghu Chen, Honghong Zhang, Ying Wang, Xiaohui Chen, Xiaochun Zheng

“…Changes in inflammatory factors and related protein expression levels in the amygdala were detected using western blotting, immunofluorescence, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The effects of intra-amygdala injections of a lentivirus targeting retinoic acid-related orphan receptor γt (RORγt) (LV-shRORγt), nifuroxazide (a STAT3 antagonist), and colivelin (a STAT3 agonist) were evaluated. …”
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Celastrol boosts fluconazole efficacy against vaginal candidiasis: in vitro and in vivo evidence by Fatma Al-zahraa A. Yehia, Hisham A Abbas, Tarek M. Ibrahim, Basem Mansour, Zuhier A. Awan, Mohammed W. Al-Rabia, Wesam H. Abdulaal, Mustafa Adnan Zeyadi, Solomon Z. Okbazghi, Tarek S. Ibrahim, Wael A. H. Hegazy, Salwa E. Gomaa

“…Synergy was spotted between celastrol and FLZ via a checkerboard assay. Quantification of m-RNA levels of efflux-mediated azole resistance genes within azole-resistant C. albicans demonstrated CDR1 overexpression. …”
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